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Abbas Saadat,AliAsghar Behnamghader,Saeed Karbasi,Daryoush Abedi,Masoud Soleimani,Abbas Shafiee 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.3
Nanocomposites have recently been identified as a useful scaffolding material in tissue engineering applications. Poly (3-hydroxybutyrate)/hydroxyapatite nanoparticles (P3HB)/(nHA) porous scaffolds were successfully fabricated through a solvent casting and particulate leaching technique. P3HB/nHA and P3HB scaffolds were prepared by the same technique for comparison. The structure of the nanocomposite and P3HB scaffolds was observed by SEM. The Energy Disperssive X-ray Analysis (EDXA, map of Ca)results indicated that HA nanoparticles were homogeneously dispersed in the P3HB matrix. X-ray diffraction (XRD)analysis showed that P3HB and HA coexist in the nanocomposite. Transmission electron microscopy (TEM)images also showed that the particle size of HA was 30 ~40 nm. The porosity of the scaffolds was 84%, and macropores and micropores coexisted and interconnected throughout the scaffolds. Acellular bioactivity experiments showed that more HA crystals formed on the surface of the nanocomposite scaffold than on the P3HB scaffold after 4weeks immersion in Simulated Body Fluid (SBF). Cell culture experiments demonstrated that the P3HB/nHA nanocomposite scaffold had a better tendency of proliferation and Alkaline Phosphatase (ALP) activity to MG 63 cells than the pure P3HB scaffold. It was found that nHA addition can improve acellular and cellular bioactivity of the P3HB scaffold.
Gadobutrol-dendrimer effects on metastatic and apoptotic gene expression
Kebriaezadeh, Abbas,Ashrafi, Sepehr,Rasouli, Rahimeh,Ebrahimi, Seyed Esmaeil Sadat,Hamedani, Morteza Pirali,Assadi, Artin,Saffari, Mostafa,Ardestani, Mehdi Shafiee Techno-Press 2016 Advances in nano research Vol.4 No.2
Dendrimers are one of the most appropriate nanocaries for imaging moieties in imaging applications.The purpose of this study was the evalution of cytotoxicity and inducing apoptosis of dendrimers. This study was conducted in order to investigate the metastasis suppression effect of dendrimer in human breast MCF-7 cell line and finding the nanoparticle protein corona in biological enviromental. Dendrimer cytotoxicity effect was assessed by MTT assay. The mRNA experession level of KAI1 as a metastasis suppressor gene, Bax as Pro- apoptotic gene, Bcl-2 as an anti-apoptotic gene and GAPDH as a housekepping gene were determined by real-time PCR assays.concentration-dependent nanoparticle cytotoxicity effect was proofed at range of 1-2 mg/mL in 24 hours, significant upregulation of mRNA expression of Bax, was observed whereas expression of anti-apoptotic Bcl-2 was down-regulated, also expression of metastasis suppressor gene KAI1 was up-regulated. So far a few studies confirmed apoptosis enhancement effect of dendrimers in MCF-7 cell line via bax/bcl-2 pathways. dendrimer nanoparticles was able to act as metastase inhibitor via upregulation of KAI1 gene.
Asghar Davood,Maryam Iman,Abbas Shafiee,Ali reza Nematollahi 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.4
1,4-Dihydropyridines have been recognized as calcium channel agonist. Three new analogues of Bay K8644 in which the ortho trifluromethyl phenyl group at position 4 is replaced by the 4-(5)-Chloro-2-ethyl-5-(4)-imidazolyl substituent, were designed and synthesized as calcium channel agonist. For this propose, the structures of designed compounds were drawn by HYPERCHEM program. Conformations of the compounds were optimized through semiempirical method followed by PM3 calculation. Then the crystalin stucture of L-type calcium channel was obtained from the Protein Data Bank (PDB) server. Docking calculations were carried out using Auto-Dock.4 program. The good interaction of our 1,4-DHP derivatives showed that they can be as possible calcium channel agonist agents. Finally compounds were synthesized according to a modified Hantzsch condensation procedure.
Roghayeh Tarasi,Ali Ramazani,Massomeh Ghorbanloo,Mehdi Khoobi,Hamideh Aghahosseini,주상우,Abbas Shafiee 대한화학회 2016 Bulletin of the Korean Chemical Society Vol.37 No.4
The manganese-containing mesoporous nanomaterial (MCM-41@PEIMn) was synthesized and used as a catalyst for the oxidation of cyclohexene, ethyl benzene, and toluene. The mesoporous MCM-41 was prepared via hydrothermal method and then polyethylenimine as a reactive surface modifier was grafted on its surface in the presence of ([3-(2,3-epoxypropoxy)propyl] trimethoxysilane) as a grafting agent. Manganese(ii) acetate was loaded onto the prepared MCM-41@PEI. The MCM-41, MCM-41@PEI, and MCM-41@PEI.Mn were characterized and elucidated by Fourier transform infrared, powder X-ray diffraction, thermogravimetric, N2 adsorption–desorption, energy-dispersive X-ray spectroscopy, scanning electron microscopy, and transmission electron microscopy techniques. The catalytic activity and selectivity of the MCM-41@PEI.Mn in the oxidation of cyclohexene, ethyl benzene, and toluene with 30% aqueous H2O2 as an oxidant were studied. Furthermore, the influences of various parameters such as temperature, oxidant amount, catalyst reusability, and kinds of solvents have been studied. The results showed that the manganese-containing mesoporous nanomaterial was easily separated from the reaction mixture and reused at least five times without noticeable loss of activity.
Maryam Iman,Ali Reza Nematollahi,Ahmad Rerza Dehpoor,Abbas Shafiee,Asghar Davood 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.9
New analogues of nifedipine, in which the ortho-nitro phenyl group at position 4 has been replaced by 4(5)-chloro-5(4)-imidazolyl substituent and which are able to interact with the receptor by hydrogen binding were designed, synthesized, and evaluated as calcium channel antagonists. The designed dihydropyridines were synthesized using the Hantzsch condensation and evaluated as calcium channel antagonists using the high K+ contraction of guineapig ileal longitudinal smooth muscle. A docking study was performed using the AutoDock4 program, and QSAR equations were obtained using multilinear regression. Our computational studies indicated that the oxygen of the ester (O10) and the N3' of the imidazole ring form a hydrogen bonding interaction with the NH of HIS 363 and NH of LYS354, respectively, and that the sum of the BEHp5 and RDF075p are the most significant descriptors. The results of calcium channel antagonist evaluation demonstrated that increasing the chain length in C3 and C5 ester substituents increased activity. The most potent compound was the bis-phenylpropyl ester (5l) derivative, in that it was more active than the reference drug nifedipine and that the bis-phenylethyl ester (5k) derivative had comparable activity with nifedipine. The present research revealed that the 4(5)-chloro-5(4)-imidazolyl moiety is a bioisoster of o-nitrophenyl in nifedipine and provided novel dihydropyridines with more activity as calcium channel antagonists.
Alireza Foroumadi,Loghman Firoozpour,Saeed Emami,Shahla Mansouri,Abdolrasoul H. Ebrahimabadi,Ali Asadipour,Mohsen Amini,Nosratollah Saeid-Adeli,Abbas Shafiee 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.2
A series of N-[5-(chlorobenzylthio)-1,3,4-thiadiazol-2-yl] piperazinyl quinolone derivatives (4a-l) have been synthesized by reaction of piperazinyl quinolones with 5-chloro-2-(chlorobenzylthio)- 1,3,4-thiadiazoles. Their structures were confirmed by elemental analysis, IR and NMR spectra. The antibacterial activities of 4a-l against a variety of Gram-positive and Gram-negative bacteria were determined. Several compounds showed a good antibacterial activity against Gram-positive bacteria among which, compound 4e with a 2-chlorobenzylthio moiety in ciprofloxacin derivative, exhibited high activities against Staphylococcus aureus and Staphylococcus epidermidis (MIC=0.06 µg/mL). The structure-activity relationship (SAR) study revealed that the position of chlorine atom on benzyl moiety would dramatically affect the antibacterial activities of the synthesized compounds.
Roghayeh Tarasi,Masoumeh Alipour,Lena Gorgannezhad,Somaye Imanparast,Aliakbar Yousefi-Ahmadipour,Ali Ramezani,Mohammad Reza Ganjali,Abbas Shafiee,Mohammad Ali Faramarzi,Mehdi Khoobi 한국고분자학회 2018 Macromolecular Research Vol.26 No.8
Three types of improved Fe3O4 magnetic nanoparticles (MNPs), including poly(amidoisophthalicacid) coated magnetite nanoparticles (Fe@PA), cyclodextrin (CD) anchored Fe@PA (Fe@PA-CD), and chitosan (Cs) coated Fe@PA-CD (Fe@PACD- Cs) were successfully developed and characterized. Laccase immobilization onto MNPs was carried out via physical adsorption. The maximal and minimal loading capacity were obtained for Fe@PA and Fe@PA-CD-Cs, respectively. Fe@PA-CDCs- laccase exhibited around 100% of the maximum activity at pH 4 and maintained 70% of its initial activity within the temperature range of 15-55 °C; and Cs coated nanoparticles were more efficient than non-coated. Fe@PA-CD-Cs-laccase maintained 70% of its initial activity up to 12 d from the first day of storage at 25 °C whereas the free laccase, Fe@PA-laccase, and Fe@PA-CD-laccase kept 10%, 28%, and 33% of initial activity, respectively. Furthermore, bio-removal of phenolic compounds was performed by the free and immobilized enzyme. Fe@PA-CD-Cs-laccase showed maximal removal with 96.4% and 85.5% for phenol and bisphenol A, respectively. It seems that Fe@PA-CD-Cs could be an appropriate support for immobilization of other enzymes in various industrial application especially bioremoval of phenolic compounds.
Cytotoxic Activity Evaluation and QSAR Study of Chromene-based Chalcones
Loghman Firoozpour,Alireza Foroumadi,Najmeh Edraki,Maryam Nakhjiri,Saeed Emami,Maliheh Safavi,Sussan Kabudanian Ardestani,Mehdi Khoshneviszadeh,Abbas Shafiee 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.12
Chalcone and chromene motifs are synthetic or naturally occurring scaffolds with significant cytotoxic profile. Two types of novel regioisomeric chromene-chalcone hybrids, namely 1-(6-chloro or 6-methoxy-2H-chromen-3-yl)-3-phenylprop-2-en-1-one (Type A) and 3-(6-chloro or 6-methoxy-2H-chromen-3-yl)-1-phenylprop-2-en-1-one (Type B), both with different substituents on the phenyl ring attached to propenone linkage, have been evaluated for their cytotoxic activity against breast cancer cell lines (MCF-7 and MDA-MB-231). The results indicate that type A of chromene-chalcones demonstrated better cytotoxic profile than type B especially in MDA-MB-231 cell line. In addition, the growth inhibitory activity of most of the target compounds is higher than Etoposide as a reference drug. QSAR analysis of these novel compounds demonstrated that topological and geometrical parameters are among the important descriptors that influence the cytotoxic activity profile of compounds.
New PLA/PEI-functionalized Fe3O4 nanocomposite: Preparation and characterization
Meisam Shabanian,Mehdi Khoobi,Farideh Hemati,Hossein Ali Khonakdar,Seyed esmaeil Sadat ebrahimi,Udo Wagenknecht,Abbas Shafiee 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.24 No.-
In this work, Fe3O4 nanoparticles were functionalized with hyperbranched polyethylenimine (PEI). The neat Fe3O4 nanoparticles (MNP) and functionalized Fe3O4 (MNP@PEI) were separately incorporated into a polylactic acid (PLA). Compared to the neat MNP, the functionalized nanoparticles (MNP@PEI) had better dispersion in the PLA matrix. The properties of the nanocomposites were strongly related to the dispersion and interaction between the nanoparticles and PLA matrix. The heat barrier effect and high nitrogen content of the MNP@PEI nanoparticles were found to be responsible for the improvement of the thermal and flame resistance properties.
Firoozpour, Loghman,Emami, Saeed,Mansouri, Shahla,Ebrahimabadi, Abdolrasoul H.,Asadipour, Ali,Amini, Mohsen,Saeid-Adeli, Nosratollah,Shafiee, Abbas,Foroumadi, Alireza 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.2
A series of N-[5-(chlorobenzylthio)-1,3,4-thiadiazol-2-yl] piperazinyl quinolones derivatives (4a-I) have been synthesized by reaction of piperazinyl quinolones with 5-chloro-2-(chlorobenzylthio)-1,3,4-thiadiazoles. Their structures were confirmed by elemental analysis, IR and NMR spectra. The antibacterial activities of 4a-I against a variety of Gram-positive and Gram-negative bacteria were determined. Several compounds showed a good antibacterial activity against Gram-positive bacteria among which, compound 4e with a 2-chlorobenzylthio moiety in ciprofloxacin derivative, exhibited high activities against Staphylococcus aureus and Staphylococcus epidermidis (MIC=0.06 ${\mu}$g/mL). The structure-activity relationship (SAR) study revealed that the position of chlorine atom on benzyl moiety would dramatically affect the antibacterial activities of the synthesized compounds.