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RISS 인기검색어
- 검색키워드
- 저자 : Masoud Soleimani (검색결과 3 건)
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Ahmadvand, Mohammad,Noruzinia, Mehrdad,Soleimani, Masoud,Abroun, Saeid Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.7
Background: Histone acetylation in chromatin structures plays a key role in regulation of gene transcription and is strictly controlled by histone acetyltransferase (HAT) and deacetylase (HDAC) activities. HDAC deregulation has been reported in several cancers. Materials and Methods: The expression of 10 HDACs (including HDAC class I and II) was studied by quantitative reverse transcription-PCR (qRT-PCR) in a cohort of mesenchymal stem cells (MM-MSCs) from 10 multiple myeloma patients with a median age 60y. The results were compared with those obtained for normal donors. Then, a coculture system was performed between MM-MSCs and u266 cell line, in the presence or absence of sodium butyrate (NaBT), to understand the effects of HDAC inhibitors (HDACi) in MM-MSCs on multiple myeloma cases. Also, the interleukin-6 (IL-6) and vascular endothelial growth factor (VEGFA) gene expression level and apoptotic effects were investigated in MM-MSCs patients and control group following NaBT treatment. Results: The results indicated that upregulated (HDACs) and downregulated (IL6 and VEGFA) genes were differentially expressed in the MM-MSCs derived from patients with multiple myeloma and ND-MSCs from normal donors. Comparison of the MM-MSCs and ND-MSCs also showed distinct HDACs expression patterns. For the first time to our knowledge, a significant increase of apoptosis was observed in coculture with MM-MSCs treated with NaBT. Conclusions: The obtained findings elucidate a complex set of actions in MSCs in response to HDAC inhibitors, which may be responsible for anticancer effects. Also, the data support the idea that MSCs are new therapeutic targets as a potential effective strategy for MM.
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Abbas Saadat,AliAsghar Behnamghader,Saeed Karbasi,Daryoush Abedi,Masoud Soleimani,Abbas Shafiee 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.3
Nanocomposites have recently been identified as a useful scaffolding material in tissue engineering applications. Poly (3-hydroxybutyrate)/hydroxyapatite nanoparticles (P3HB)/(nHA) porous scaffolds were successfully fabricated through a solvent casting and particulate leaching technique. P3HB/nHA and P3HB scaffolds were prepared by the same technique for comparison. The structure of the nanocomposite and P3HB scaffolds was observed by SEM. The Energy Disperssive X-ray Analysis (EDXA, map of Ca)results indicated that HA nanoparticles were homogeneously dispersed in the P3HB matrix. X-ray diffraction (XRD)analysis showed that P3HB and HA coexist in the nanocomposite. Transmission electron microscopy (TEM)images also showed that the particle size of HA was 30 ~40 nm. The porosity of the scaffolds was 84%, and macropores and micropores coexisted and interconnected throughout the scaffolds. Acellular bioactivity experiments showed that more HA crystals formed on the surface of the nanocomposite scaffold than on the P3HB scaffold after 4weeks immersion in Simulated Body Fluid (SBF). Cell culture experiments demonstrated that the P3HB/nHA nanocomposite scaffold had a better tendency of proliferation and Alkaline Phosphatase (ALP) activity to MG 63 cells than the pure P3HB scaffold. It was found that nHA addition can improve acellular and cellular bioactivity of the P3HB scaffold.
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Azadeh Izadyari Aghmiuni,Saeed Heidari Keshel,Mostafa Rezaei-Tavirani,Farshid Sefat,Arash Khojasteh,Masoud Soleimani,Farzad Pakdel 한국섬유공학회 2022 Fibers and polymers Vol.23 No.12
Tissue engineering provides new approaches to improve skin lesions. However, cell differentiation onto theengineered substrate with the skin-like pattern is the main challenge. Here we have tried to fabricate such the substrate viastudying the change in polymers ratios and molecular weight, and grafting scaffold with silk fibroin (SF) biomaterial. To thisend, chitosan and PEG were mixed at the volume ratios of 25:75, 50:50, and 65:35, and samples were lyophilized by thefreeze-drying method. Based on the result, the ratio of 65:35 indicated better physicomechanical properties than two otherscaffolds. Afterward, Chi/PEG scaffolds were prepared via mixing chitosan/PEG with (65:35) and PEG molecular weights of2000, 4000, 6000, 10000 Da. It was found that the increase of PEG molecular weight (>4000) was led to the reduction intensile strength and elongation of the scaffold network. Hence, PEG4000 was selected as the optimum molecular weight todesign SF-grafted Chi/PEG scaffold. Therefore, Chi/PEG4000-SF scaffold was designed to evaluate the volume ratio of SF(1 %, 3 %, 5 %) and compare data with the decellularized dermis. The results showed Chi/PEG4000-SF(3%) scaffold not onlywas led to the same elongation as Chi/PEG-SF(5%) scaffold but also created the dermis-like modulus. Moreover, Chi/PEGSF(3%) provided higher expression level of keratinocytes (bio-mimetic pattern) than decellularized dermis due to betterphysicomechanical properties. Hence, it seems that engineered scaffolds can be a more suitable option than native tissue (dueto removal of limitations such as donor sites and immunogenicity, and their mechanical properties). This study can providenovel insight into the better design of skin-engineered scaffolds.
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