홍국균의 최적배양조건에 관한 연구

정순택,임종환,박양균,황금택,박현진,안찬영,강성국,문길만 木浦大學校 工業技術硏究所 1994 工業技術硏究誌 Vol.4 No.-

Cell growth and pigment productivity of Monascus anka KCCM 11832 and Monascus sp. has isolated from the Korean Maeju(soybean koji) were determined for effect of culture medium composition and incubation conditions. Increase of NH₄NO₃, and KH₂PO₄ concentration as mineral source promoted mycelium growth, and the highest cell growth was showed in the medium with 0.05% KH₂PO₄ when C/N ratio is 20:1, but pigment production was significantly inhibited at higher concentration than this level. Cell growth and pigment production in porato dextrose medium were higher than those in czapeck-dox broth and malt extract medium. In terms of temperature, the highest cell growth was showed at 34℃, but pigment productivity was the highest at 26℃.

Ontogeny of the locomotive behavior in a subsocial spider

Kil Won Kim,Soon Kil Ahn,Kyoung Hye Kim 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10

Post-maternal social organisation of Amaurobius ferox (Araneae, Amaurobiidae) provides an opportunity to investigate the mechanisms of social behaviour in spiderlings while controlling for potential effects of maternal presence. Development and characteristics of locomotory behaviours in young individuals of the subsocial Amaurobius ferox were examined. Young collectively show a very rapid and linear locomotion in a short distance in response to an intruder in the natal nest. Such a locomotory behaviour of the spiderlings resembles the successive synchronised stepping towards prey of the permanently social spider, Anelosimus eximius. We observed the development of different types of locomotion relative to the age of young, collective tendency (i.e., proportion of individuals within the clutch engaging in the behaviour), and directional properties of the locomotion. Locomotory behaviours of spiderlings fell into two categories: ‘abrupt locomotion’ (AL), involving fast and linear movement over a short distance; and ‘ordinary locomotion’ (OL), involving slower movement over longer distances and times. The frequency of AL (abrupt locomotion) did not vary much throughout the days post-matriphagy, occurring for a limited period of time. On the other hand, the frequency of OL (ordinary locomotion) varied over the days post-matriphagy and increased during the maternal social period.

Antiangiogenic Anticancer Agent, CKD-732

( Soon Kil Ahn ),( Chong Kun Dang ),( Chong Kun Dang ) 전남대학교 약품개발연구소 2004 약품개발연구지 Vol.13 No.-

항암제의 발달과 연구개발 현황

안순길 ( Soon Kil Ahn ) 대한임상종양학회 2007 Korean Journal of Clinical Oncology Vol.3 No.2

Priming 처리에 의한 토마토 종자의 발아력과 Membrane integrity 에 미치는 영향

안종길,조동,강점순,손병구,최영환,조정래 密陽産業大學校 農業技術開發硏究所 1997 農業技術開發硏究所報 Vol.1 No.1

Priming은 신속하고 균일한 발아 유도 및 초기 생육 촉진으로 육묘에 투입되는 노력과 비용을 절감할 수 있다. 본 연구에서는 토마토 종자의 priming 및 발아과정 중 세포막의 기능이 종자활력에 미치는 영향을 구명하기 위하여 일련의 실험을 수행하였다. 토마토 종자의 적정 priming 처리제는 150mM의 KNO₃였고 priming은 발아소요일수를 단축시켰으며, 그 효과는 저온에서 더욱 현저하였다. KNO₃로 priming은 처리과정중 처리 용액에서 해리되어 있던 K+ 이온이 종자내로 이동하였다. Priming 처리기간중 전기전도도는 발아속도 단축에 가장 효과적이었던 KNO₃에서 처리개시 직후 약간 낮았다가 그후 최종일까지 일정한 수준을 유지하였다. 발아기간중 용액의 전지전도도는 KNO₃priming 종자는 낮았으나 K₃PO₄priming 종자는 높았다. 발아촉진에 가장 효과적이었던 150mM KNO₃은 priming 기간처리중 단백질, 아미노산, 당의 유출량은 K₃PO₄ 및 浸漬종자에 비해 낮았으며, 그 효과는 발아시에도 유지되었다 Seed priming is a useful technique for early establishment of uniform seedlings. Priming is suited to overcome or lessen the impact of a number of soil and climatic constraints, including cold and wet conditions, drought, and incidence of diseases. The objective of this study was to find membrane integrity change during priming and germination of tomato seeds. Among the five different chemicals, KNO₃at 150mM gave the shortest T50 (days to 50% of the final germination percentage). Compared to unprimed seeds, those seeds primed with 150mM KNO₃at 20℃ for 4 days reduced T50 values when germinated at 15℃ and 20℃. It was concluded that seed priming was an effective way for rapid and synchronized germination especially at low temperature. Changes in conductivity of priming solutions during the 4-day period of priming were highly dependent upon the priming agents. Conductivity of the KNO₃and K₃PO₄solution slowly declined during the first 3 hours, and then increased. Amount of amino acids, sugars and proteins exuded from seeds into KNO₃solution was less than that into distilled water and K₃PO₄. This result suggested that the KNO₃priming played a positive role in regulating the permeability of cell membranes.

인위적 노화에 의한 고추종자의 단백질, 아미노산, 당 및 무기성분의 유출과 종자활력 회복을 위한 priming 효과

안종길,손병구,조동,최영환,강점순 密陽産業大學校 農業技術開發硏究所 1999 農業技術開發硏究所報 Vol.3 No.1

노화처리 일수가 경과하여 활력이 저하된 종자일수록 종자에서 유출되는 단백질, 아미노산, 당의 유출량이 많았다. 20일간 노화처리된 종자는 침지 4일째 종자당 35.8 mg의 단백질이 유출되어 건전종자에 비해 6.9배 많았다. 아미노산 유출량도 노화처리된 저활력 종자에서 증가하였다. 당의 유출량도 20일 노화처리된 저활력 종자에서 침지 4일째 종자당 36.5g의 당이 유출되어 건전종자보다 2.8배 높았다. 무기성분의 유출량은 저활력 종자에서 많았고 전체적인 무기성분의 유출량은 단백질, 아미노산, 및 당보다 상대적으로 낮았다. 유출량에 근거하여 비파괴적으로 종자활력을 추정할수 있는 가용성 물질은 단백질과 K였다. 노화처리된 종자는 발아율과 발아세가 저하되었으며, 종자활력이 저하된 노화종자를 priming 처리함으로써 종자활력을 복원시킬 수 있었다. 이러한 현상은 저활력 종자를 저온에서 발아시킨 경우에 더욱 뚜렷하였다. Quantity of protein, amino acid and sugar leaked from seeds was greater as the viability of seeds was dropped by the time elapsed of aging seed treatment. In the seeds with the artificial aging treatment for 20 days, 35.8mg of protein was leaked on the 4th day after soaking and was 6.9 tinges higher than that of the control. Leakage of amino acid was also higher from the low viable seed treated with the aging treatment. For the leakage of sugar from the seeds with the aging treatment for 20 days, 36,5g was leaked on the 4th day after soaking, and was 2.8 times higher than that of the control. For the leakage of inorganic compound, it was higher from the low viable seeds, and leakage of total inorganic compound was relatively lower than protein, amino acid and sugar. According to the quantity of leakage, water soluble compound, which can be used for the assessment of seed viability without any destruction, were protein and potassium. Germination rate and percentage of seeds were respectively dropped with the aging seed treatment, and the seed viability could recovered by priming treatment. This phenomenon was certainly clear when the low viable seeds were genminated at law temperature.

Diary! ether: Synthesis and biological evaluation as antimitotic agents

AHN soon Kil,LEE Mi-sung,IN Jin-Kyung,YANG Jun geun,KWAK Jae-Hwan,LEE Heesoon,JUNG Jae-Kyung,BOOVANAHALLI Shanthaveerappa K .,LEE Kyeong,CHOI Nam Song,LEE sungsook,MOON Seung Kee,KIM Soo Jin 대한약학회 2006 大韓藥學會 總會 및 學術大會 Vol.2006 No.2

고추와 토마토 종자의 발아력 증진에 미치는 Osmotic Priming 및 Solid Matrix Priming의 효과

안종길,강점순,조동 密陽産業大學校 農業技術開發硏究所 1999 農業技術開發硏究所報 Vol.3 No.3

Osmotic priming과 SMP 처리는 종자 발아력을 증진시키는 종자처리이다. Osmotic priming과 SMP 처리과정중 작물별 수분흡수율은 처리한 3시간 이내에 대부분의 수분흡수가 이루어졌다. 최종처리일의 두 처리간 종자함수율은 고추종자에서는 큰 차이는 없었으나, 토마토에서는 osmotic priming이 SMP보다 2.4% 높았다. Osmotic priming과 SMP 처리가 고추와 토마토에서 발아율을 향상시키지는 못했다. 그러나 osmotic priming과 SMP 처리는 평균발아소요일수(MDG) 및 50% 발아에 소요되는 일수(T50)은 단축되어 조기발아를 유도하였다. 고추종자에서는 osmotic priming과 SMP 처리는 무처리 종자에 비하여 T50 이 15℃에서 약 6일, 20℃에서는 약 5일, 25℃에서는 약 4.6일, 30℃에서는 약 4일 정도 단축되었고, 토마토 종자에서는 15℃에서 3.3일, 20℃에서는 1.7일, 25℃에서는 약 4.6일, 30℃에서는 0.5일 정도 단축되었다. 이러한 발아촉진 효과는 불량발아 조건인 저온에서 더욱 뚜렷하였고, 발아적온에 근접할수록 미약해지는 경향이었다. Osmotic priming과 SMP 상호처리간 발아촉진 효과는 고추에서는 SMP 처리가 좋았고, 토마토에서는 큰 차이가 없었다. 종자처리 후 건조방법은 고추는 완전건조가 토마토에서는 표면건조가 조기발아 하는 경향이었다. Osmotic and solid matrix priming treatments enhance germination performance. We compared osmotic with solid matrix priming to determine which was the more effective treatment for improving seed germination in pepper and tomato. Pepper seeds were osmotic primed with 200 mM K₃PO₄and solid matrix were primed with a ratio of 4.0g seed: 1.2 g Micro Cel E: 7.8 ml water for 7 days at 20℃. Tomato seeds were osmotic primed with 150 mM KNO₃and solid matrix primed with a ratio of 4.0 g seed: 1.2 g Micro Cel E: 7.0 ml water for 4 days at 20℃. Seed hydration was immediately observed after osmotic priming and solid matrix priming treatment. The moisture content of solid matrix primed seeds was lower than that of osmotic primed seeds in two vegetable crops. Osmotic priming and solid matrix priming did not increased percent germination, but showed shorter number of days to 50% of the final germination percentage(T50 ) than those untreated seeds regardless of germination temperature. T50 value was reduced in osmotic or solid matrix primed pepper seeds about 6.0, 5.0, 4.6 and 4.0 days than those untreated seeds at 15, 20, 25, and 30℃, respectively. While, that in tomato seeds was reduced about 3.3, 5, 4.6 and 4.0 days than those untreated seeds at 15, 20, 25, and 30℃, respectively. The effectiveness of osmotic priming or solid matrix priming in reducing the T50 was greater when the seeds were germinated at 15℃ than at temperature of higher than 20℃. Solid matrix primed seeds germinated faster than osmotic primed seeds at all temperature in pepper. However, there was no significant difference in seed germination rate between solid matrix and osmotic primed seeds in tomato. After priming, dried-back seeds showed faster germination than surface-dried seeds in pepper. On the other hand, ther was no significant difference in tomato.

자발성 두개강내 추골동맥 박리의 진단 및 치료

안경순,김성민,김용한,심영보,송준호,오세문,최선길 대한신경외과학회 1996 Journal of Korean neurosurgical society Vol.25 No.6

The authors have dealt with six cases of spontaneous intracranial vertebral artery dissection during the past 3 years. Most of the patients were in their fourth or fifth decade of life, and men predominated. All except one patient had severe antecedent headache and half of the 6 cases were hypertensive. The presenting clinical features were subarachnoid hemorrhage. Wallenberg's syndrome, brain stem RIND (reversible ischemic neurologic deficit) and cerebellar infarct. The locations of the dissection were right vertebral artery in four patients, right vertebral and basilar artery in one and left vertebral artery in another one. Most of the patients showed typical intramural hematoma or double lumen on the MRI. On the MR angiography, irregular segmental narrowing of vertebral artery or invisible pathologic vertebral artery were demonstrated. The angiographic findings included alternating irregular stenotic and dilated segment(pearl and string sign), aneurysmal dilatation occulusion etc. Four patients were treated medically while the other two were treated surgically by proximal vertebral artery clipping of the affected artery. Upon follow-up, the outcome were excellent in two good in two and fair in two.

광질이 토마토 plug tray 묘의 생육에 미치는 영향

안종길,강점순,손병구,최영환,이용재 密陽大學校 生命資源開發硏究所 2003 生命資源開發硏究 Vol.7 No.-

(시험1)착색 셀로판필름과 (시험2)적색광(R)/원적색 광(FR)비 필름을 이용한 토마토묘의 광질반응을 구명 하기 위하여 토마토(挑太郞 8)를 공시재료로 하여 3월 23일에 파종하고 4월 4일~4월 30일간에 (시험1)적색, 청색, 녹색, 황색, 무색투명의 5종류의 착색 셀로판필름과 (시험2)적색광/원적색광 필름을 플러그 트레이 (128공) 육묘상에 터널식 피복처리하여 수행한 결과 (시험1) 셀로판필름의 광투과율이 무색투명, 황색의 순으로 가장 높았고 다음은 적색, 청색, 녹색의 순이었다. 토마토의 초장 및 엽면적등의 생육은 적색이 약간 작았고 녹색, 청색은 초세가 약하여 도장성을 나타내었으나 황색, 무색투명구에서는 묘가 크고 튼튼하였다. 생체중 및 건물중은 지상부, 지하부 모두 황색, 무색 투명군는 컸으나 적색, 청색, 녹색은 작았고 엽록소함량도 적었으며 초세도 연약하였다. 개화기는 황색, 무색투명, 적색의 순으로 빨랐으나 청색, 녹색은 매우 지연되었다. (시험2)R/FR필름의 광투과율이 관행보다 낮았고 토마토묘의 초장이 약간 억제되었으며 엽면적, 생체중 및 건물중도 낮았다. This study was conducted to clarify effect of five cellophane film such as red, blue, green, yellow and transparent color and R/FR film on growth in tomato seedlings(lycopersicum esculentum Mill. cv. Momotaro 8). The tomato was sown in plug tray with 128 hole on March 23, and (Exp.1) five cellophane film and (Exp.2) R/FR film were covered over tomato seedlings from April 4 to April 30, respectively. (Exp.1) The rate of light transmission of each film was the highest in transparent cellophane, and followed in order of yellow, red, blue and green cellophane. The plant height and leaf area were small in red cellophane, green and blue cellophane was weak or rapidly growth, however the plant height in yellow and transparent cellophane was vigor. The fresh and dry weight of seedlings were higher in yellow and transparent cellophane than in red, blue and green cellophane, and also the chlorophyll content was same tendency. The flowering date was earlier in order of yellow, transparent and red cellophane, but was very retarded in blue and green cellophane. (Exp.2) The rate of light transmission was lower in R/FR film than control. The plant height, leaf area, fresh weight and dry weight were small in R/FR film.