자궁내막암 환자의 재발여부 확인에 있어 FDG-PET scan 의 유용성에 대한 고찰

홍성진 ( Hong Seong Jin ),이삼미 ( Lee Sam Mi ),박천숙 ( Park Cheon Sug ),김호아 ( Kim Ho A ),김법종 ( Kim Beob Jong ),김문홍 ( Kim Mun Hong ),최석철 ( Choe Seog Cheol ),유상영 ( Yu Sang Yeong ),이경희 ( Lee Gyeong Hui ) 대한산부인과학회 2004 Obstetrics & Gynecology Science Vol.47 No.2

목적 : Inhibin은 α-subunit와 β-subunit로 구성된 이질 이량체의 당단백으로 β-subunit의 차이에 의해 inhibin A (α-βA)와 inhibin B (α-βA)로 구분된다. 여성에서 inhibin은 주로 난소의 과립막세포 및 황체에서 생산되어 뇌하수체의 FSH 분비를 억제한다고 알려져 왔으나, 임신중 태반과 남성의 고환에서도 생산된다는 사실이 밝혀져 다양한 생리적 작용이 추정되고 있으며 최근에 inhibin A와 B를 분리 측정할 수 있는 방법이 개발되어 inhibin에 대한 연구가 활발히 진행되고 있다. 이에 저자들은 정상 월경주기를 가진 가임기 여성, 폐경이행기 여성, 폐경후기 여성에서 inhibin A와 inhibin B의 농도를 측정하여 월경주기에 따른 정상치의 변화와 노화에 따른 농도 변화를 관찰하고, 난소에서 면역조직화학적 염색법으로 inhibin A와 inhibin B의 발현 양상과 변화를 비교 관찰하여 inhibin의 정확한 생리작용, 폐경이행기에서 inhibin의 역할 및 폐경이행기와 폐경의 예측 지표의 사용 가능성을 알아보고자 하였다. 연구 방법 : 월경주기와 난소기능이 정상이면서 난소종양이 없는 정상 가임기 여성 160명, 폐경이행기 여성 60명, 폐경후기 여성 20명에서 정맥혈을 채혈하여 혈중 inhibin A와 inhibin B 농도를 ELISA로 측정하였고, 그중 정상 가임기 여성 35명, 폐경이행기 여성 20명, 폐경기 여성 5명의 난소 조직을 inhibin α, βA 및 βB subunit에 대한 단클론 항체를 이용하여 면역조직화학적 염식을 시행하였다. 결과 : 1. 정상 가임기 여성에서 혈중 inhibin A 농도는 중증식기까지 낮은 농도를 유지하다 후증식기 (16.53±1.57 pg/ml)부터 증가하기 시작하여, 중분비기 (45.85±2.08 pg/ml)에 최고치에 이른 후 서서히 감소하는 경향을 보였다. Inhibin B 농도는 전증식기 (65.40±4.08 pg/ml)부터 증가하기 시작하여 증식기 동안 계속 높은 농도를 유지하다가 배란기 (110.74±9.83 pg/ml)에 최고치에 이른 다음 분비기에 급격히 감소하였다. 2. 폐경이행기 여성에서 혈중 inhibin A 농도는 증식기에 6.68±0.53 pg/ml, 분비기에 21.78±3.61 pg/ml로 모두 정상 가임기 여성과 비교하여 유의하게 낮았다 (P<0.01). Inhibin B의 농도는 증식기에 52.16±7.46 pg/ml, 분비기에 22.41±6.73 pg/ml로 모두 정상 가임기 여성과 비교하여 유의하게 낮았다 (각각 P<0.01, P=0.025). 3. 폐경후기 여성에서 inhibin A와 inhibin B 농도는 모두 검출되어 않았다. 4. 정상 가임기 여성에서 inhibin α subunit의 면역염색반응은 과립막세포, 난포막세포 및 황체에서 강하게 발현되었다. Inhibin βA의 면역염색반응은 난포에서는 거의 발현되지 않았으나 황체에서는 관찰되었다. Inhibin βB의 면역염색반응은 일차난포에서 처음 발현되어 성장난포와 성숙난포의 과립막세포와 난포막세포에서 관찰되었으나 Inhibin α보다는 약했고 황체에서는 거의 관찰되지 않았다. 5. 폐경이행기 여성에서 inhibin subunit들의 면역염색반응은, 정상 가임기 여성과 같은 양상으로 발현되었으나, 발현정도는 약했다. 모든 면역염색반응은 과립막세포보다 난포막세포에서 더 강하게 나타났다. 6. 폐경후기 여성에서 inhibin subunit들의 면역염색반응은 전혀 관찰되지 않았다. 결론 : Inhibin A는 후증식기 성숙난포에서 처음 생산되기 시작하여 배란 후 황체에서 주로 생산되며, Inhibin B는 일차난포에서 생성되기 시작하여 배란 시까지 분비되므로 inhibin A는 황체기능을, inhibin B는 난포의 기능을 반영한다. 폐경이행기가 되면 inhibin A와 B의 분비는 급속히 감소하게 되어 폐경 후에는 난소에서 생성되지 않는다. 따라서 inhibin A와 B는 여성의 월경주기에 따라 생산과 분비가 서로 다른 호르몬으로 난자 성숙과 난포발달에 관여하며, 난소의 노화 정도를 측정하는 폐경이행기의 지표로 이용될 수 있을 것이다. Objective : To understand the physiologic effects and secretion pattern of inhibin A and inhibin B during menstrual cycle and menopausal transition, inhibin A and inhibin B levels were measured. And to detect any changes in expression of inhibins in human ovary with age, we examined immunohistochemical staining of α, βA, and βB subunits of inhibin in ovarian tissues. This study was also designed to investigate whether or not inhibin is an early marker for menopausal transition. Methods : Inhibin A and inhibin B levels were measured in 320 samples from normal reproductive women, in 60 from perimenopausal women, and in 20 from menopausal women by ELISA. And we examined the immunohistochemical staining of α, βA, and βB subunits of inhibin in ovarian tissues of 35 normal reproductive, 20 perimenopausal, and 5 menopausal women, respectively. Results : In the normal reproductive women, inhibin A begins to increase in the late proliferative phase (16.53±1.57 pg/ml), reaches the peak in the mid-secretory phase (45.85±2.08 pg/ml), reaches the peak in the ovulatory phase (110.74±9.83 pg/ml), reaches the peak in the ovulatory phase (110.74±9.83 pg/ml), and thereafter declines rapidly. In the perimenopausal women, mean inhibin A serum concentration was 6.68±0.53 pg/ml during proliferative phase and 21.78±3.61 pg/ml during secretory phase, which were significantly lower than that of the same phase in the normal reproductive women (P<0.01). Mean inhibin B serum concentration was 52.16±7.46 pg/ml during proliferative phase and 22.41±6.73 pg/ml during secretory phase, which were significantly lower than that of the same phase in the normal reproductive women (P<0.01, P=0.025). In the menopausal women, both inhibin A and inhibin B were not detected. In the normal reproductive women, we observed strong immunostaining for α subunit in granulosa cells, theca cells, and corpus luteum. Immunostaining for βA subunit was observed in corpus luteum, but not in growing follicles. Immunostaining for βB subunit was observed in primary follicle, granulosa and theca cells of growing follicle, and mature follicle, but less strong than immunostaining for α subunit. No staining for βB subunit was observed in the corpus luteum. In the perimenopausal women, immunostaining for inhibin subunits were observed in the same pattern as that of the normal reproductive women, but weaker. Stronger immunostaining was observed in theca cells than granulosa cells. In the menopausal women, none of the immunostaining of inhibin subunits were observed.

UPLC-PDA를 이용한 창포류의 분류 및 함량 분석

조지은(Ji Eun Jo),이아영(A Yeong Lee),김효선(Hyo Seon Kim),문병철(Byeong Cheol Moon),지윤의(Yunui Ji),천진미(Jin Mi Chun),김호경(Ho Kyoung Kim) 한국식품과학회 2013 한국식품과학회지 Vol.45 No.3

A quantitative method using ultra performance liquid chromatography with a photodiode array detector (UPLCPDA) was established for the analysis of 2 major plant metabolites: β-asarone and α-asarone from Acorus gramineus, A. tatarinowii, A. calamus and Anemone altaica, and their contents are compared with other herbs of Acorus species. The method was validated according to the International Conference on harmonization (ICH) guideline for validation of analytical procedures with respect to precision, accuracy, and linearity. The average content of β-asarone in Acorus gramineus was significantly higher than that in others, with the second highest concentration observed in A. tatarinowii, and only a trace amounts found in A. calamus and Anemone altaica. In contrast, the average content of α-asarone in A. calamus was the highest, followed by that in Acorus gramineus and A. tatarinowii. principle component analysis (PCA) confirmed that β-asarone and α-asarone content differed among the species. These results suggest that this UPLC-PDA method can be considered as good quality control criteria for Acorus gramineus.

부산지역 유흥업소 종사여성으로부터 분리된 HPV16형의 발암유전자(E6/E7) 돌연변이 유형 분석

민상기(Sang-Kee Min),김성순(Sung Soon Kim),최병선(Byeong-Sun Choi),장대호(Dai-Ho Jang),이미옥(Mee-Ok Lee),최성화(Seung-Hwa Choi),김남호(Nam-Ho Kim),박연경(Yon-Koung Park),정영아(Yeong-A Jeong),김성준(Seong-Joon Kim),빈재훈(Jae-Hun Bi 한국생명과학회 2009 생명과학회지 Vol.19 No.6

HPV-16형의 염기배열 변이는 지역적, 인종적으로 특징적인 차이가 있으며 특히 HPV-16형 E6/E7 유전자의 특정 염기서열변이는 자궁경부암 및 자궁상피내 신생종양물의 발생을 일으키는 고위험 요인으로 알려져 있다. 본 연구는 2007년 부산지역 유흥업소 종사여성으로 분리된 HPV-16형 19건을 대상으로 E6/E7 유전자 영역(nt 34-880)을 표적으로 지역적 염기서열 변이를 조사하였다. nucleotide 수준에서 HPV16형 E6 유전자는 T178G (n=11), T178A (n=1), T350G (n=4), A442C (n=2), A104T, A111G, C116T, G145T, T183G, C335T, G522C 등 11종의 변이주가 발견되었고, E7 유전자는 A647G (n=12), A645C, A777C, G663A, T732C, T760C, A775T, T789C, T795G 등 9종의 변이주가 발견되었다. 아미노산 수준에서는 HPV-16형 E6 단백질의 경우 D25E (n=12), L83V (n=4), E113D (n=2), M1L, Q3R, P5S, Q14H, D25N, I27R, H78Y, C140S 등 11종의 변이주를, HPV16형 E7 단백질의 경우 N29S (n=12), L28F, T72S 등 3종의 변이주를 관찰할 수 있었다. 본 연구 결과, 부산지역의 HPV-16형 E6/E7 우점 돌연변이주는 E6 D25E (75%), E7 N29S (78%)로 각각 나타났다. 앞으로 자궁경부암 환자 및 일반여성을 포함한 더 많은 모집단을 대상으로 HPV-16형 E6/E7의 intratypic variants를 비교 조사하여 실제 HPV-16형 E6/E7 어떤 변이주가 자궁경부암 유발 위험성과의 관련성은 더 많이 연구되어져야 할 것으로 사료된다. Recent studies have reported that the distribution of HPV-16 sequence variation differs geographically, and more specifically that HPV-16 E6/E7 intratypic variants might carry a high risk for development of ICC (invasive cervical cancer) and CIN (cervical intraepithelial neoplasia) in a given population. To investigate the genetic diversities of HPV-16 E6/E7 oncogene by region, we collected nineteen HPV-16 isolates from sexually high-risk women in Busan, and analyzed the HPV-16 E6/E7 coding regions (nt 34 to 880) with HPV-16 E6/E7 specific PCR amplification. At the nucleotide level, eleven variants of the E6 genes and nine variants of the E7 genes were identified as follows: E6 T178G (n=11), E6 T178A (n=1), E6 T350G (n=3), E6 A442C (n=2), E6 A104T, E6 A111G, E6 C116T, E6 G145T, E6 T183G, E6 C335T, E6 G522C and E7 A647G (n=12), E7 A645C, E7 A777C, E7 G663A, E7 T732C, E7 T760C, E7 A775T, E7 T789C and E7 T795G, respectively. At the amino acid level, the isolated HPV-16 E6 and E7 genes showed eleven E6 variants: E6 D25E (n=12), E6 L83V (n=4), E6 E113D (n=2), E6 M1L, E6 Q3R, E6 P5S, E6 Q14H, E6 D25N, E6 I27R, E6 H78Y, E6 C140S and three E7 variants: N29S (n=12), L28F, T72S. HPV16 E6 L83V, the dominant variant in the Caucasian population, showed relatively low frequencies in our study population. We elucidated that the dominant HPV-16 E6/E7 variants were HPV-16 E6 D25E (63.2%) and HPV-16 E7 N29S (63.2%), which were phylogenetically included in Asian lineage. Further study is needed to evaluate the risk of cervical cancer related HPV-16 E6/E7 intratypic variants in the Korean population.

예비유아교사를 위한 디지털 역량 증진 교육 프로그램 개발 및 효과

박소영,권승아,김민지,김한빛,이혜린,한나경,윤소망,정문경 이화여자대학교 교육과학연구소 2023 교육과학연구 Vol.54 No.4

본 연구는 예비유아교사 대상의 디지털 역량 증진 교육 프로그램을 ADDIE 모형에 근거해 개발하고 효과를 분석함으로써, 예비유아교사가 향후 교육현장에서 디지털 매체와 테크놀로지를 수업설계, 교수ㆍ학습과 놀이지원 등에 원활하게 적용하고 발현적으로 활용할 수 있도록 하는 데 목적을 두고 있다. 이를 위해 선행연구 및 문헌고찰을 통해 예비유아교사의 디지털 역량을 디지털 기기의 교수적 활용, 디지털 정보 활용, 디지털 콘텐츠 제작 및 플랫폼 활용, 디지털 페다고지로 설정하고, 각 역량별로 하위역량을 설정하였다. 또한 학습자 분석을 위해 A대학 유아교육학과 2, 3학년에 재학 중인 106명의 학생을 조사하였고, 교육 프로그램의 설계 및 개발 단계에서는 전문가 7인의 두 차례 검토를 거쳐 교육목표와 교육내용, 수업 주제와 방법, 수업의 구체적인 내용을 도출하였다. 최종 교육 프로그램은 총 5주간 10시간에 걸쳐 총 45명의 예비유아교사를 대상으로 시행되었으며, 교육의 효과성 및 학습자의 내면 변화를 파악하기 위해 37개 문항으로 구성된 디지털 역량 측정도구로 교육 전과 후로 2회 평가를 시행하였으며, 교육 종료 후 학습자의 성찰일지를 분석하였다. 교육 후 모든 디지털 역량 및 하위역량에서 교육의 효과가 있는 것으로 나타났으며, 성찰일지 분석 결과 교육 프로그램 이수 후 디지털 역량과 관련해 학습자 내면의 긍정적 변화가 일어난 것을 확인할 수 있었다. 향후 예비유아교사가 유아교육기관에서 디지털 매체와 최신 테크놀로지를 활용하여 교육 활동을 수행하기 위해서는 실제와 경험을 기반으로 한 디지털 역량 교육이 필요하며, 이에 대한 교육적 대안을 제시했다는 점에서 연구의 의의가 있다. This study aimed to develop an educational program to promote the digital competence of pre-service early childhood teachers based on the ADDIE model and help them apply digital media and technologies smoothly to their lesson designs, teaching and learning, and play supports in the field of education and make use of them in an emergent manner. For these purposes, the study reviewed previous studies and pieces of literature and set the digital competence of pre-service early childhood teachers as the instructional utilization of digital devices, the utilization of digital information, digital content creation and platform utilization, and digital pedagogy before defining the subcompetences of each competence. For the analysis of learners, the researchers surveyed 106 sophomores and juniors in the Department of Early Childhood Education at A University in Korea. In the educational program design and development stage, researchers identified educational goals and content, lesson topics and methods, and the specific content of a lesson based on two reviews by seven experts. The final educational program was implemented with 45 pre-service early childhood teachers for a total of ten hours over five weeks. They took an evaluation test before and after educational program with a digital competence inventory of 37 items to determine its effectiveness and the internal changes of the learners, whose self-reflection journals were analyzed after the program ended. The findings show that the students experienced the effects of education in all the types of digital competence and their subcompetences after receiving the training. The analysis results of the learners’ self-reflection journals indicate that the educational program positively affected their digital. Pre-service early childhood teachers need to receive digital competence training based on current realities and experience so that they can provide educational activities with digital media and the latest technologies in early childhood education institutions. The present study is significant in that it proposed such an educational alternative.

The association between prion proteins and Aβ1-42 oligomers in cytotoxicity and apoptosis

Hyeon, Jae Wook,Kim, Su Yeon,Park, Jun Sun,Choi, Bo Yeong,Lee, Sol Moe,Ju, Young Ran,An, Seong Soo A.,Kim, Chi-Kyeong Elsevier 2012 Biochemical and biophysical research communication Vol.424 No.2

<P>Misfolding of prion protein (PrP to PrPSc) can cause neurodegenerative prion diseases. As a glycosylphosphatidylinositol (GPI)-anchored membrane protein, the normal form of PrP (PrPC) can function as a receptor for ligands in the extracellular space. PrPC was suggested to be involved in memory, synaptic neuronal communication, and anti-oxidation as a neuroprotective agent. The recently identified interaction between PrPC and Aβ(1-42) oligomers suggested another role for PrP as a receptor for Aβ(1-42) oligomers, thereby influencing cytotoxicity and apoptosis. Here, the association between PrPC and Aβ(1-42) oligomers was investigated by visualizing protein localization in neuronal cells by immunocytochemistry. Aβ(1-42) oligomer-induced cytotoxicity was tested in respective expressions of PrPC by using mouse neuroblastoma-2a (N2a) cells, the prion protein overexpressed cells (L2-2B1), and a Prnp-null mouse hippocampal cell line (HpL 3-4). Moreover, apoptotic proteins such as caspase-8 were used to assess the effect of PrPC on Aβ(1-42) oligomer-mediated apoptosis. In L2-2B1 and HpL 3-4 cells, the difference in the cytotoxicity of Aβ(1-42) oligomers could be clearly distinguished. In addition, Aβ(1-42) oligomers induced mitochondria dysfunction, reactive oxygen species (ROS) generation, and calcium influx PrPC-dependently. Apoptosis, related to mitochondria dysfunction, was further investigated to determine the cytotoxic pathway; the results suggest that PrPC could be involved in both the intrinsic and extrinsic apoptotic pathways. Finally, cells with abundant PrPC expression seemed to be more susceptible to Aβ(1-42) oligomer toxicity, suggesting the importance of the level of PrPC expression in the induction of apoptosis.</P>

Anti-tumor Activity of the Ethyl Acetate Fraction from Asparagus cochinchinensis in HepG2-xenografted Nude Mice

Chun, Jin-Mi,Cheon, Myeong-Sook,Moon, Byeong-Cheol,Lee, A-Yeong,Choo, Byung-Kil,Kim, Ho-Kyoung The Korean Society for Applied Biological Chemistr 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.4

Although Asparagus cochinchinensis Merrill (Liliaceae) has traditionally been used for cancer treatment, its in vivo antitumor efficacy has not been established. Anti-tumor effect of the ethyl acetate fraction of A. cochinchinensis extract (EAF-ACE) was evaluated in nude mice xenografted with human hepatocellular carcinoma (HepG2) cells. Serum biomarkers were also examined to monitor the hepatotoxicity and nephrotoxicity of EAF-ACE. HepG2-xenografted nude mice were randomly divided into untreated group, EAF-ACE-treated groups (50, 100, or 200 mg/kg), and positive control group (3 mg/kg cisplatin). All groups were assayed for tumor mass formation, apoptosis induction, and serum biomarkers. EAF-ACE treatment significantly reduced tumor growth. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay revealed EAF-ACE treatment significantly increased the number of apoptotic cells in tumors in a dose-dependent manner, whereas cisplatin treatment significantly decreased tumor volumes and increased the number of apoptotic cells, and exhibited hepatotoxicity and nephrotoxicity, in contrast to EAF-ACE treatment. EAF-ACE was found to possess antitumor activities without associated hepatotoxicity and nephrotoxicity. Therefore, EAF-ACE may be effectively used as a chemopreventive agent.

Anticancer Activity of Asparagus cochinchinensis Extract and Fractions in HepG2 Cells

Park, Mi-Kyung,Cheon, Myeong-Sook,Kim, Seong-Hwan,Chun, Jin-Mi,Lee, A-Yeong,Moon, Byeong-Cheol,Yoon, Tae-Sook,Choo, Byung-Kil,Kim, Ho-Kyoung The Korean Society for Applied Biological Chemistr 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.2

Asparagus cochinchinensis Merrill (Liliaceae) has been traditionally used for the treatment of cancer in Korea and China, but its anticancer activity and underlying mechanism remain to be defined. Anticancer activities were investigated on fractions obtained from A. cochinchinensis 70% ethanol extract (ACE-EtOH) in human hepatocellular carcinoma HepG2 cells. Ethylacetate fraction from A. cochinchinensis extract (ACE-EA), more effective than other fractions, induced apoptosis of HepG2 ($IC_{50}=72.33{\pm}0.34{\mu}g/mL$), as revealed by apoptotic feature observation, increased capase-3 activity and Poly ADP ribose polymerase cleavage, and decreased expression of X-linked inhibitor of apoptosis protein in a dose-dependent manner. Protein levels of autophagy-related molecules, microtubule-associated protein 1 light chain 3 ${\alpha}$ and beclin 1, appeared to be induced by ACE-EA, suggesting ACE-EA exhibits anti-cancer activity with induction via both apoptosis and autophagy signaling pathways in HepG2 cells.

Anti-tumor Activity of the Ethyl Acetate Fraction from Asparagus cochinchinensis in HepG2-xenografted Nude Mice

( Jin Mi Chun ),( Myeong Sook Cheon ),( Byeong Cheol Moon ),( A Yeong Lee ),( Byung Kil Choo ),( Ho Kyoung Kim ) 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.4

Although Asparagus cochinchinensis Merrill (Liliaceae) has traditionally been used for cancer treatment, its in vivo antitumor efficacy has not been established. Anti-tumor effect of the ethyl acetate fraction of A. cochinchinensis extract (EAF-ACE) was evaluated in nude mice xenografted with human hepatocellular carcinoma (HepG2) cells. Serum biomarkers were also examined to monitor the hepatotoxicity and nephrotoxicity of EAF-ACE. HepG2-xenografted nude mice were randomly divided into untreated group, EAF-ACE-treated groups (50, 100, or 200 mg/kg), and positive control group (3 mg/kg cisplatin). All groups were assayed for tumor mass formation, apoptosis induction, and serum biomarkers. EAF-ACE treatment significantly reduced tumor growth. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay revealed EAF-ACE treatment significantly increased the number of apoptotic cells in tumors in a dosedependent manner, whereas cisplatin treatment significantly decreased tumor volumes and increased the number of apoptotic cells, and exhibited hepatotoxicity and nephrotoxicity, in contrast to EAF-ACE treatment. EAF-ACE was found to possess antitumor activities without associated hepatotoxicity and nephrotoxicity. Therefore, EAF-ACE may be effectively used as a chemopreventive agent.

Anticancer Activity of Asparagus cochinchinensis Extract and Fractions in HepG2 Cells

( Mi Kyung Park ),( Myeong Sook Cheon ),( Seong Hwan Kim ),( Jin Mi Chun ),( A Yeong Lee ),( Byeong Cheol Moon ),( Tae Sook Yoon ),( Byung Kil Choo ),( Ho Kyoung Kim ) 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.2

Asparagus cochinchinensis Merrill (Liliaceae) has been traditionally used for the treatment of cancer in Korea and China, but its anticancer activity and underlying mechanism remain to be defined. Anticancer activities were investigated on fractions obtained from A. cochinchinensis 70% ethanol extract (ACE-EtOH) in human hepatocellular carcinoma HepG2 cells. Ethylacetate fraction from A. cochinchinensis extract (ACE-EA), more effective than other fractions, induced apoptosis of HepG2 (IC50=72.33±0.34 μg/mL), as revealed by apoptotic feature observation, increased capase-3 activity and Poly ADP ribose polymerase cleavage, and decreased expression of X-linked inhibitor of apoptosis protein in a dose-dependent manner. Protein levels of autophagy-related molecules, microtubule-associated protein 1 light chain 3 α and beclin 1, appeared to be induced by ACE-EA, suggesting ACE-EA exhibits anti-cancer activity with induction via both apoptosis and autophagy signaling pathways in HepG2 cells.

Effects of Aralia continentalis and Angelica biserrata on Inflammatory Response in Lipopolysaccharide-Induced RAW 264.7 Macrophages and Phorbol Ester-induced Ear Edema

Myeong Sook Cheon,윤태숙,Seung Ju Kim,Goya Choi,문병철,A-Yeong Lee,Byung Kil Choo,Ho Kyoung Kim,So Yeon Yu,Ken Yasukawa 한국응용생명화학회 2009 Journal of Applied Biological Chemistry (J. Appl. Vol.52 No.2

The roots of both Aralia continentalis and Angelica biserrata, known as ‘Dokwhal’ in Korea, have been used widely as a traditional oriental medicine to treat inflammation and thrombosis. However, the pharmacological differences between A. continentalis and A. biserrata have not been fully established. In the present study, we investigated and compared the inhibitory effects of 70% ethanolic extracts of A. continentalis (ACE) and A. biserrata (ABE) on the production of inflammatory mediators and secondary swelling from chemically induced ear edema. In RAW264.7 macrophages, both ACE and ABE significantly inhibited the release of nitric oxide, prostaglandin E2, interlukin-1beta, and tumor necrosis factor-alpha in a dose dependent manner. In addition, the swelling from TPA-induced edema in mouse ears was reduced by ACE and ABE. Overall, ACE showed stronger activities than ABE in vitro and in vivo. Our results indicate that A. continentalis roots possess stronger anti-inflammatory activity than A. biserrata roots.