5종 방제의 항비만 효과 비교

천진미,최고야,김동선,성윤영,노경진,김승형,김호경,Chun, Jin Mi,Choi, Goya,Kim, Dong-Seon,Sung, Yoon-Young,Nho, Kyoung Jin,Kim, Seung-Hyung,Kim, Ho Kyoung 대한한의학방제학회 2013 大韓韓醫學方劑學會誌 Vol.21 No.2

Objectives : Samhwangsasim-tang (SST), Hwangryeonhaedok-tang (HHT), Ukgan-san (UGS), Onjunghwadam-hwan (OHH) and Samul-tang (SMT) have been used for the treatment of various diseases. This study was performed to compare the anti-obesity effects of 5 herbal formulas in high fat diet-(HFD) induced obese mice. Methods : The mice were randomly divided into seven groups that were fed a normal diet (ND), a HFD, a HFD plus SST (HFD + SST), a HFD plus HHT (HFD + HHT), a HFD plus UGS (HFD + UGS), a HFD plus OHH (HFD + OHH), or HFD plus SMT (HFD + SMT) at 300 mg/kg/day for 7 weeks. All groups were assayed for body weights, food efficiency ratio (FER), final liver and fat weight and blood biochemical parameters. Results : The increased body weights, food efficiency ratio (FER), and serum total triglyceride were decreased in HFD + OHH group relative to the same measurements in HFD group. Furthermore, the HFD + SST group significantly reduced FER, liver and abdominal subcutaneous fat weight gains, and serum total triglyceride, whereas HDL-cholesterol level was increased compared to HFD group. Conclusions : These results suggested that HFD + OHH and HFD + SST exert anti-obesity effects in HFD-induced obese mice.

한약재 품질관리에 관한 인식도 조사연구

천진미(Jin-Mi Chun),이아영(A-Young Lee),전원경(Won-Kyung Jeon),추병길(Byung-Kil Choo),이혜원(Hye-Won Lee),김홍준(Hong-Jun Kim),김호경(Ho-Kyoung Kim) 대한한의학회 2006 대한한의학회지 Vol.27 No.2

고혈압에 사용되는 한약처방 5종의 항비만 효과

천진미(Jin Mi Chun),김동선(Dong Seon Kim),최고야(Goya Choi),성윤영(Yoon Young Sung),박선행(Sun Haeng Park),김승형(Seung Hyung Kim),김호경(Ho Kyoung Kim) 한의병리학회 2013 동의생리병리학회지 Vol.27 No.5

유통한약 품질규격 모니터링 연구

김호경,천진미,이아영,이혜원,최지현,장설,고병섭,Kim, Ho-Kyoung,Chun, Jin-Mi,Lee, A-Young,Lee, Hye-Won,Choi, Ji-Hyun,Jang, Seol,Ko, Byoung-Seob 한국한의학연구원 2005 한국한의학연구원논문집 Vol.11 No.2

This study was investigated to determine the quality control of Oriental medicine from stores dealing in Oriental medicine around Seoul and Daegu. We tested total 120 samples that widely used 15 species in herbal medicine (Lycii Fructus, Platycodi Radix, Angelicae Gigantis Radix and 12 others) being collected from Oriental medicine clinic, pharmacy, Oriental pharmacy, Oriental medical hospital and Oriental drug store. We have estimated Oriental medicine by K.P. (Korean Pharmacopoeia), K.H.P(Korean Herbal Pharmacopoeia) and announcement of KFDA. The items of examination were identification, purity, loss on drying, ash, acid insoluble ash, extract content, essential oil content, assay, heavy metal limit, and pesticides residue(BHC, DDT, Aldrin, Endrin, Dieldrin). As a result, 20 samples in total 120 samples were not satisfied with the standard and 7 species in total 15 species were not satisfied with the standard. Identification test, extract content test and pesticides residue(BHC, DDT, Aldrin, Endrin, Dieldrin) were satisfied with the standard. The result will be the basic data for the quality control of Oriental medicine.

한약재의 미생물허용한도 설정을 위한 모니터링(I)

이주현,전원경,고병섭,천진미,이아영,김호경,Lee, Ju-Hyun,Jeon, Won-Kyung,Go, Byoung-Seob,Chun, Jin-Mi,Lee, A-Yeong,Kim, Ho-Gyoung 한국한의학연구원 2006 한국한의학연구원논문집 Vol.12 No.1

한약재는 생산, 가공, 유통과정에서 적절한 보관. 관리가 이루어지지 않으면 미생물 증식 가능성이 크고 이로 인한 부패나 변질이 우려되며 한약 품질 저하를 가져올 수 있다. 한약재 미생물허용한도에 대한 각 나라별 규격기준을 보면 유렵약국, 도일약국에서는 호기성균의 미생물오염한도(microbial contamination limit)를 10 CFU/g 이하로 규격화하고 있다. 또한 미국약국 NF(US phamacopeia & National Formulary)에서는 생약의 미생물 한도치를 유럽약국과 같은 정도의 규격설정을 하고 있다. 특히 일본의 경우 일본약국방(日本藥局方) 및 국방외생약규격집(局方外生藥規格集)에 의해서 한약의 품질을 유지하고 있고, 미생물학적 품질관리 실태조사가 체계적으로 이루어지고 있다. 본 연구에서는 3개 지역에서 각각 1곳의 약업사를 정하여 대상품목 총 30종을 선정하였다. 실험의 재료는 문헌과 자료조사에 의해 미생물에 의한 오염이 용이한 품목 18종, 육진약(六陳藥)에 속하는 6종, 그리고 육미지황환을 구성하는 6종을 구입하여 대한약전 8개정의 미생물한도 시험법에 따라 시험하였다. 한약재에 대한 미생물 오염 실태조사를 통하여 미생물 허용한도를 설정하는데 기초 자료로 제시하고자 한다. 한약재 미생물 오염 실태조사 결과 WHO 미생물한도 기준을 적용하였을 때, 전체적으로 진균의 오염이 높은 경향을 보였고 향후 한약재에 대한 미생물오염도 모니터링을 지속적으로 하여야 할 것으로 사료된다. Objectives : This study has attempted to establish an organized system for the microbiological quality of raw materials which are important factors in preparing the standard for microbial limits. Methods : This study has first set up microbial contamination limit test. total aerobic microbial count and total fungi count, int accordance with testing method of the Korea Pharmacopeia 8th edition in order to establish an inspection standard for microbial contamination. Results : The microbial contamination of 18 items that are highly prone to contamination by three regions(Seoul, Daejeon, Gyeongsangbuk-do), As a result, Morus alba Linne and Rehmannia glutimosa Liboschitz var. purpurea Makino showed as high contaminated by WHO's Microbial Contamination Limit standard. In case of Yukjin medicine in the Theory of Herb Medicinal Properties, total bacterial contamination rate showed as 17.7%, total fungal contamination rate showed as 41.2% and total aerobic mircobial count and total fungi count on Ephedra sinica Stapf. Pinellia ternate Breitenbach, Evodia officinalis Dode showed as high measured. The microbial conatmination rate materials which make up Yukmijihwanghwan were mostly high therefore the total aerobic microbial count was measured as high in case of Yukmijihwanghwan, the characteristics about microbial contamination strain is to be researched. Conclusions : By combining the basic data and experimental results related to microbial contamination of herb medicine, the most ideal storage standards for herb medicine has been attempted to be presented.

한방처방의 전탕 전과 후의 위해물질 농도변화 -보양.보음 처방을 중심으로 -

서창섭,황대선,이준경,하혜경,천진미,엄영란,장설,신현규,Seo, Chang-Seob,Huang, Dae-Sun,Lee, Jun-Kyoung,Ha, Hye-Kyoung,Chun, Jin-Mi,Um, Young-Ran,Jang, Seol,Shin, Hyeun-Kyoo 대한한의학방제학회 2009 大韓韓醫學方劑學會誌 Vol.17 No.2

Objective : To compare the contents of heavy metals, residual pesticides and sulfur dioxide before/after a decoction. Methods : The heavy metal contents before/after a decoction were measured by Inductively Coupled Plasma Atomic Emission Spectrometer (ICP-AES) and mercury analyzer. In order to analyze pesticides in 4 samples we used simultaneous multi-residue analysis of pesticides by GC/ECD, which was followed by GC/MSD analysis to confirm the identity of the detected pesticide in each sample. In addition, the contents of sulfur dioxide ($SO_2$) were performed by Monier-Williams distillation method. Results: 1. The mean values of heavy metal contents (mg/kg) for the samples were as follows: Jaeumganghwa-tang (before decoction - Pb; 1.190, Cd; 0.184, As; 0.099 and Hg; 0.028, after decoction - Pb; .033, Cd; 0.003, As; 0.005 and Hg; 0.001), Yukmijiwhang-tang (before decoction - Pb; 0.484, Cd; 0.133, As; 0.053 and Hg; 0.009, after decoction - Pb; 0.065, Cd; 0.008, As; 0.007 and Hg; not detected), Bojungikgi-tang (before decoction - Pb; 0.863, Cd; 0.197, As; below 0.016 and Hg; 0.011, after decoction - Pb; 0.071, Cd; 0.009, As; 0.004 and Hg; 0.001) and Ssangwha-tang (before decoction - Pb; 1.511, Cd; 0.212, As; 0.094 and Hg; 0.016, after decoction - Pb; 0.029, Cd; 0.006, As; 0.005 and Hg; 0.0004). 2. Contents (mg/kg) of sulfur dioxide ($SO_2$) before a decoction in Jaeumganghwa-tang, Yukmijiwhang-tang and Ssangwha-tang exhibited 22.7, 107.3 and 5.5, respectively. However, contents of sulfur dioxide after a decoction in all samples were not detected. 3. Contents (mg/kg) of residual pesticides before/after a decoction in all samples were not detected. Conclusion : These results will be used to establish a criterion of heavy metals, residual pesticides and sulfur dioxide.

간암 세포주 HepG2에 대한 맥문동탕(麥門冬湯)추출물의 항암 및 항전이 효능

전명숙 ( Myeong Sook Cheon ),천진미 ( Jin Mi Chun ),윤태숙 ( Tae Sook Yoon ),이아영 ( A Yeong Lee ),문병철 ( Byeong Cheol Moon ),추병길 ( Byung Kil Choo ),김성환 ( Seong Hwan Kim ),김호경 ( Ho Kyoung Kim ) 대한본초학회 2009 大韓本草學會誌 Vol.24 No.3

Objectives: Maekmoondong-tang (MMDT), a Korean herbal medicine, has been used to treat severe dry cough in patients with bronchitis and pharyngitis. MMDT has been reported to have anti-inflammatory, anti-allergic, immunomodulatory, secretory-modulating, and metabolic regulatory actions. However, there are no evidence in regard to the effects of MMDT on carcinogenesis and metastasis. Here, we investigated the effects of 70% ethanol extract of MMDT on cell viability, apoptosis, and motility in human hepatocarcinoma HepG2 cells. Methods: Cell viability was measured using the CCK-8 assay, and the apoptosis induction was evaluated by caspase-3 activity. To detect apoptotic features, the cells treated with MMDT were stained with 4`-6-diamidino-2-phenylindole (DAPI). Cell motility was examined by Boyden chamber assay and Real-time Cell Index of Migration assay. Gelatin zymography also performed to measure matrix metalloproteinase (MMP)-2/9 activity. Results: We found that MMDT significantly inhibited cell proliferation and increased caspase-3 activity in a dose-dependent manner in HepG2 cells. Apoptotic features such as chromatin condensation and apoptotic bodies were observed in MMDT-treated cells by DAPI staining. MMDT also suppressed PMA-induced cell motility and activities of MMP-2/9. Conclusions: Our results exhibited that MMDT possess the anti-carcinogenetic and anti-metastatic activities via caspase-3 activation and down-regulation of cell motility and invasion in HepG2 cells. Therefore, these findings suggest that MMDT could be potentially applied to the prevention and treatment of cancer.

중금속, 잔류농약 및 잔류이산화황의 전탕 전, 후의 농도 변화 - 다빈도 태음인 사상처방을 중심으로 -

서창섭,황대선,이준경,하혜경,천진미,엄영란,장설,김종열,이시우,신현규,Seo, Chang-Seob,Huang, Dae-Sun,Lee, Jun-Kyoung,Ha, Hye-Kyoung,Chun, Jin-Mi,Um, Young-Ran,Jang, Seol,Kim, Jong-Yeol,Lee, Si-Woo,Shin, Hyun-Kyoo 사상체질의학회 2009 사상체질의학회지 Vol.21 No.1

1. Objectives To compare the contents of heavy metals, residual pesticides and sulfur dioxide before/after decoction. 2. Methods The heavy metal contents before/after decoction were measured by Inductively Coupled Plasma Atomic Emission Spectrometry (ICP-AES) and mercury analyzer. In order to analyze pesticides in 3 samples we used simultaneous multi-residue analysis of pesticides by GC/ECD, followed by GC/MSD analysis to confirm the identity of the detected pesticide in each sample. In addition, the contents of sulfur dioxide (SO2) were performed by Monier-Williams distillation method. 3. Results 1) The mean values of heavy metal contents (mg/kg) for the samples were as follows: Yuldahanso-tang (before decoction - Pb; 1.85, Cd; 0.148, As; 0.042 and Hg; 0.003, after decoction - Pb; 0.096, Cd; 0.006, As; 0.006 and Hg; 0.002), Chongsimyonja-tang (before decoction - Pb; 1.193, Cd; 0.094, As; 0.084 and Hg; 0.008, after decoction - Pb; 0.053, Cd; 0.007, As; 0.011 and Hg; not detected) and Taeyeumjowee-tang (before decoction - Pb; 0.878, Cd; 0.078, As; 0.302 and Hg; 0.004, after decoction - Pb; 0.079, Cd; 0.005, As; 0.006 and Hg; not dectcted). 2) Contents (mg/kg) of residual pesticides before/after decoction in all samples were not detected. 3) Contents (mg/kg) of sulfur dioxide (SO2) before decoction in Yuldahanso-tang, Chongsimyonja-tang and Taeyeumjowee-tang exhibited 6.1, 37.8, 31.5 and 19.7, respectively. However, contents of sulfur dioxide after decoction in all samples were not detected. 4. Conclusion These results will be used to establish a criterion of heavy metals, residual pesticides and sulfur dioxide.

한의학 기반 반려동물용 치료기술 개발 관련 특허 분석

이지혜,김성호,이수은,남현화,박준홍,천진미,서윤수,김보혜,이정민,문창종,김중선,문병철,Lee, Ji Hye,Kim, Sung Ho,Lee, Sueun,Nam, Hyeon-Hwa,Park, Jun Hong,Chun, Jin Mi,Seo, Yun-Soo,Kim, Bohye,Lee, Jeongmin,Moon, Changjong,Kim, Joong Sun,Moon 대한본초학회 2022 大韓本草學會誌 Vol.37 No.6

Objective : In this study, we conducted to analysis the patents about Korean medicine based treatment technology for pet. Methods : We collected the Korean, Japan, USA, China, EU and PCT patent for the Korean medicine-based treatment technology for pet using WIPSON data base registered by October 4, 2020 were selected. Results : Patents for the development of pet Korean medicine-based treatment technology have been constantly applied since the mid-2000s, starting in 1990, with a total of 84 applications. The number of patent applications filed in Korea was the largest, after Japan and China. The detailed composition of the applications reported until now is pharmaceutical compositions for the treatment of pet were the most applied with 39%(33 cases), followed by feed used as stocks for pet at 36%(30 cases). According to the patent application rate by disease, most of applications rate composed to be associated with immunity in 2009 to 2013 with 40% of applications. In 2014 to 2018 43% of patent applications are associated with gastrointestinal/digestive system. In this regard, multiple applications were filed in the order of immunity, digestive system, obesity and urinary system. Conclusion : This study analyzed the trend of 84 patents about Korean medicine-based treatment technology for pet to date to establish the basis for future research and development. There were many applications related to immunity from 2009 to 2013, and since then, related to digestive disease from 2014 to 2018.

3T3 - L1 지방세포에서 녹나무 잎 추출분획물이 인슐린작용에 미치는 효과

고병섭(Byoung-Seob Ko),이미영(Mi Young Lee),김호경(Ho Kyoung Kim),천진미(Jin Mi Chun),최수봉(Soo Bong Choi),전동화(Dong Wha Jun),장진선(Jin Sun Jang),박선민(Sunmin Park) 한국식품영양과학회 2005 한국식품영양과학회지 Vol.34 No.9

일본에서는 녹나무 줄기에서 분리한 정유의 하나인 camphor는 강심제 등 약용으로 사용하고 있지만, 녹나무 잎 추출물에 대한 연구도 적고 특히 항당뇨 효과에 대한 연구는 거의 없었다. 본 연구에서는 녹나무 잎 추출물이 in vitro에서 인슐린 작용, 인슐린 분비 또는 탄수화물의 소화에 효과적인지를 조사함으로 항당뇨에 효과적인지 여부를 조사하였다. 녹나무 잎을 70% 에탄올로 추출한 후 메탄올과 물을 섞은 용액으로 단계별로 XAD-4 column으로 분획하였다. 녹나무 잎 추출물은 고농도(1 ㎎/mL)에서도 MTT로 측정하였을 때 세포 독성을 나타내지 않았다. 녹나무 잎 추출분획물 중 40과 60% 메탄올층은 3T3-L1 지방세포에 처리하였을 때 인슐린의 작용을 향상시켜 포도당의 흡수를 증가시키는 효과가 매우 컸다. 이 추출분획물들은 3T3-L1 지방세포에 존재하는 인슐린의 작용을 향상시켜 인슐린을 10 nM을 처리한 것보다도 효과적으로 포도당 흡수를 증가시켰다. 또한 40% 메탄올충은 3T3-L1 섬유아세포에 분화 유도물질를 처리하였을 때 PPAR-γ인 pioglitazone과 마찬가지로 지방 세포로의 분화를 촉진시키고 지방의 축적도 증가시켰다. 그러므로 40% 메탄올층에는 PPAR-γ agonist로 작용하는 물질이 함유되어 있을 가능성이 높다. 베타세포라인인 Min6 세포에 녹나무 잎 추출분획물을 처리한 후 저농도와 고농도 포도당 자극시 인슐린 분비를 측정하였을 때 두 농도에서 모두 인슐린 분비에 영향을 미치지 않았다. 또한 녹나무 잎 추출분획물은 탄수화물의 소화에 작용하는 효소인 α-glucoamylase의 활성에 영향을 미치지 않았다. 결론적으로 녹나무 잎에는 인슐린 분비나 탄수화물의 소화에 관여하는 성분의 없지만, 인슐린 작용을 향상시키는 인슐린 민감성 물질이 함유되어 있을 가능성이 높다. 특히 40% 메탄올층에는 PPAR-γ agnist로 작용하는 인슐린 민감성 물질이 존재할 가능성이 높다. In the present study, we screened candidates for enhancing insulin action and secretion from Cinnamomum camphora (CC) fractions, in 3T3-L1 adipocytes and Min6 cells by investigating insulin-stimulated glucose uptake and glucose-stimulated insulin secretion, respectively. CC were extracted by 70% ethanol followed by XAD-4 column chromatography with serial mixture solvents of methanol and water, and the fractional extractions were utilized for determining insulin action and secretion, and α-glucoamylase suppressing activity. A significant insulin-stimulated glucose uptake was observed in 3T3-L1 adipocytes, giving 0.5 or 5 ㎍/mL of 40% and 60% methanol fractions plus 0.2 nM insulin, compared to the treatment of DMSO plus 0.2 nM insulin. The treatments of 40% and 60% methanol fractions plus 0.2 nM insulin reached the glucose uptake of 10 nM insulin treatment. The 40% methanol fraction increased triglyceride accumulation by stimulating differentiation and triglyceride synthesis similar to pioglitazone, PPAR-γ agonist. No inhibition of α-glucoamylase activity of CC fractions was observed. They did not modulate the insulin secretion capacity in either low or high glucose media. These results suggest that 40% methanol fraction contains a potential insulin sensitizer to have a similar function of PPAR-γ agonist. Crude CC extract may improve glucose utilization by enhancing insulin-stimulated glucose uptake without elevating glucose stimulated insulin secretion.