UPLC-PDA를 이용한 창포류의 분류 및 함량 분석

조지은(Ji Eun Jo),이아영(A Yeong Lee),김효선(Hyo Seon Kim),문병철(Byeong Cheol Moon),지윤의(Yunui Ji),천진미(Jin Mi Chun),김호경(Ho Kyoung Kim) 한국식품과학회 2013 한국식품과학회지 Vol.45 No.3

A quantitative method using ultra performance liquid chromatography with a photodiode array detector (UPLCPDA) was established for the analysis of 2 major plant metabolites: β-asarone and α-asarone from Acorus gramineus, A. tatarinowii, A. calamus and Anemone altaica, and their contents are compared with other herbs of Acorus species. The method was validated according to the International Conference on harmonization (ICH) guideline for validation of analytical procedures with respect to precision, accuracy, and linearity. The average content of β-asarone in Acorus gramineus was significantly higher than that in others, with the second highest concentration observed in A. tatarinowii, and only a trace amounts found in A. calamus and Anemone altaica. In contrast, the average content of α-asarone in A. calamus was the highest, followed by that in Acorus gramineus and A. tatarinowii. principle component analysis (PCA) confirmed that β-asarone and α-asarone content differed among the species. These results suggest that this UPLC-PDA method can be considered as good quality control criteria for Acorus gramineus.

사이토카이닌과 에틸렌, 프로피코나졸 복합처리에 따른 고년근 인삼 생육 및 진세노사이드 함량 변화

권나영(Na Yeong Kwon),이정우(Jung Woo Lee),정성민(Sung Min Jung),조익현(Ick Hyun Jo),장인복(In Bok Jang),방경환(Kyong Hwan Bang),김영창(Young Chang Kim),김동휘(Dong Hwi Kim),황현아(Hyeon A Hwang),임유경(Yoo Kyung Lim),조현우(Hyun Woo 한국약용작물학회 2023 한국약용작물학회지 Vol.31 No.1

Background: Korean ginseng (Panax ginseng C. A. Meyer) is recognized for its medicinal properties. Because ginseng is cultivated for 4 – 6 years in one place, the growing environment can affect ginseng growth. Specifically, root growth can be altered by plant hormones, which in turn, affects crop yields. Among plant hormones, cytokinin is known to increase secondary growth by promoting cambium cell division. In addition, ethylene and propiconazole can regulate root development. In this study, the effects and interactions of cytokinin, ethylene, and propiconazole on four-year-old ginseng plants were analyzed. Methods and Results: Ginseng plants were treated with each hormone every two weeks. Cytokinin (6-benzylaminopurine, 500 μM) was applied to the soil, and 100 nM of propiconazole or 100 μM of ethylene was applied in combination with cytokinin. Root width and weight, and ginsenoside content of each plant were the highest when cytokinin and propiconazole were applied together. The highest number of buds was observed under cytokinin treatment. However, no significant differences were observed in shoot growth, except for stem width. Conclusions: The greatest effect on root development was observed when cytokinin and propiconazole were applied together, and ginsenoside content increased following hormone treatment.

TiO₂ 나노입자 광촉매 반응에 의한 비스페놀 A의 분해 제거 및 독성 저감

조아영,정진호,Jo, A-Yeong,Jung, Jinho 응용생태공학회 2015 Ecology and resilient infrastructure Vol.2 No.4

본 논문은 수용액에서 $TiO_2$ 나노입자 (Degussa P25) 광촉매 반응에 의한 비스페놀 A (BPA)의 분해 제거를 연구하였다. 3시간의 광촉매 반응 (자외선 파장 = 365 nm, 자외선 강도 = $3mW\;cm^{-2}$, $TiO_2$ 농도 = $2.0g\;L^{-1}$)에 의하여 98%의 BPA ($1.0{\times}10^{-5}M$)와 89%의 총유기탄소가 제거되었다. 그리고 광분해, 가수분해와 흡착반응에 의한 BPA의 분해 제거는 각각 2%, 5%와 13%로 나타났다. 광촉매 반응에 의한 BPA의 분해 제거는 수산화 라디칼의 소광제인 메탄올의 농도가 증가 할수록 감소하였다. 이것은 BPA와 수산화 라디칼의 반응이 BPA 분해 제거의 주요한 기작이라는 것을 나타낸다. 이 반응의 초기 유사 1차 속도 상수는 $7.94{\times}10^{-4}min^{-1}$로 계산되었으며, BPA 90%를 분해 제거하는 시간은 25분으로 나타났다. 그리고 광촉매 반응에 의한 BPA의 독성 저감을 평가하기 위하여 물벼룩 (Daphnia magna, 생후 24시간 미만)을 이용한 급성독성 시험을 실시하였다. 물벼룩에 대한 BPA의 급성독성 (48시간)은 초기 2.93 TU (독성 단위)였으며, 3시간의 광촉매 반응 후에는 무독성으로 나타났다. 이것은 BPA의 광촉매 반응에서 독성 분해산물이 생성되지 않는 다는 것을 제시한다. Photocatalytic degradation of bisphenol A (BPA) in aqueous solution was investigated using $TiO_2$ nanoparticles (Degussa P25) in this study. After a 3 hr photocatalytic reaction (${\lambda}=365nm$ and $I=3mW\;cm^{-2}$, $[TiO_2]=2.0g\;L^{-1}$), 98% of BPA ($1.0{\times}10^{-5}M$) was degraded and 89% of the total organic carbon was removed. In addition, BPA degradation by photolytic, hydrolytic and adsorption reactions was found to be 2%, 5% and 13%, respectively. The reaction rate of BPA degradation by photocatalysis decreased with increasing concentration of methanol that is used as a hydroxyl radical scavenger. This indicates that the reaction between BPA and hydroxyl radical was the key mechanism of BPA degradation. The pseudo-first-order reaction rate constant for this reaction was determined to be $7.94{\times}10^{-4}min^{-1}$, and the time for 90% BPA removal was found to be 25 min. In addition, acute toxicity testing using Daphnia magna neonates (< 24 h old) was carried out to evaluate the reduction of BPA toxicity. Acute toxicity (48 hr) to D. magna was decreased from 2.93 TU (toxic unit) to non-toxic after photocatalytic degradation of BPA for 3 hr. This suggests that there was no formation of toxic degradation products from BPA photocatalysis.

부스트 컨버터의 모델링과 단일 루프 & 이중 루프 제어기의 성능 비교

조영민(Jo Yeong-Min),이주아(Lee Ju-A),김용래(Kim Yong-Rae),김수빈(Kim Soo-Bin),송승호(Song Seung-Ho),최주엽(Choi Ju-Yeop) 한국태양에너지학회 2021 한국태양에너지학회 논문집 Vol.41 No.4

To maximize the amount of power generated in a photovoltaic inverter, MPPT (Maximum Power Point Tracking) is an essential function of a photovoltaic (PV) inverter. A PV inverter generally consists of a DC/DC converter (boost converter) and a DC/AC inverter. For stable MPPT control, the controller of the boost converter must be properly designed. In this study, the nonlinear characteristics of solar cells were linearized and modeled for a proper controller design, and an analog controller was designed using MATLAB and converted into a digital controller. The controller was simulated using a designed controller based on PSIM, and the performances of a single-loop controller and dual-loop controller were compared. In addition, the performance of the controller was verified and compared experimentally by applying the designed controller to a commercial PV inverter.

Simultaneous Determination of Seven Compounds in Houttuynia cordata using UPLC-PDA

Hyo Seon Kim,A Yeong Lee,Jun Yeon Park,Ji Eun Jo,문병철,Jin Mi Chun,ki Jung Kil,Ho Kyoung Kim 한국생약학회 2012 Natural Product Sciences Vol.18 No.3

A UPLC method for the simultaneous determination of seven compounds was established for the quality control in H. cordata. The UPLC was performed on a C18 HSS T3 2.1 ? 100 mm, 1.8 mm column during a 13 minute gradient elution of 0.2% aqueous acetic acid and acetonitrile with the flow rate of 0.2 mL/min at 30 oC. The UPLC method was validated according to the ICH guideline of analytical procedures with respect to precision, accuracy, and linearity. The limit of determination and quantitation for the seven compounds were 0.01-0.09 and 0.03-0.28 mg/mL, respectively. The calibration curves of all seven compounds showed good linearity (r2 > 0.999). The intra-day and inter-day the RSD values used to evaluate the precision of analysis were less than 0.9%. The recoveries of quantified compounds ranged from 98.63 to 103.85%. The developed UPLC method was found to be effective, convenient and sensitivity for quantitative analysis of seven compounds in H. cordata. This work could be provided a baseline source for quality control of H. cordata.

A Flexible Translation-Based Knowledge Graph Embedding Adapting Unobserved Entities

A-Yeong Kim,Seong-Bae Park,Sang-Jo Lee 보안공학연구지원센터 2016 International Journal of Software Engineering and Vol.10 No.11

This paper proposes a flexible translation-based knowledge graph embedding that learns unobserved entities by moving positions of embedding vectors from existed embedding space. To reflect unobserved entities, previous methods tend to learn knowledge graphs all over again. This process causes high cost of calculation. Thus, this paper introduces an adjusting method which moves positions of learned embedding vectors according to unobserved entity. This idea is based on TransE model that is a one of translation-based methods. According to experiments, the proposed method shows the plausibility at link prediction task and triple classification task. These experimental results prove that reducing learning cost is a crucial issue for embedding knowledge graphs.

HPLC-PDA를 이용한 반하, 호장남성, 수반하의 분류 및 함량분석

조지은 ( Ji Eun Jo ),이아영 ( A Yeong Lee ),김효선 ( Hyo Seon Kim ),문병철 ( Byeong Cheol Moon ),최고야 ( Go Ya Choi ),지윤의 ( Yun Ui Ji ),김호경 ( Ho Kyoung Kim ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.5

Objectives: A quantitative method using high performance liquid chromatography with a photodiode array detector(HPLC-PDA) was established for the quantitative analysis of the four main compound and pattern analysis to classification Piiellia ternate, P. pedatisecta and Typhonium flagelliforme. Methods: The analytical procedure for the determination of P. ternata, together with the known main compounds uracil, uridine, guanosine and adenosine was established. Optimum HPLC-PDA separation of these P. ternata was possible on Luna C18(2) column material, using water and acetonitrile as mobile phase. The method was validated according to regulatory guidelines. In addition, this assay method were analyzed for the content of four main compound in P. ternata, P. pedatisecta and T. flagelliforme and by data obtained from the HPLC-PDA analysis was performed principal component analysis(PCA). Results: Validation results indicated that the HPLC method is well suited for the determination of the roots of P. ternata with a good linearity (r2> 0.999), precision and recovery rates. Analysis of HPLC-PDA, the average content of uracil, uridine, guanosine and adenosine was significantly higher in P. ternate>P. pedatisecta> T. flagelliforme order. The application of PCA to main compound data by HPLC-PDA permitted the effective discrimination among the three species. Conclusions: Analysis of both HPLC-PDA and PCA confirmed the fact that four main compound and pattern profiles of P. ternata, P. pedatisecta and T. flagelliforme were different from each other.

난소암에서 IGF-2 와 H19 gene 의 promoter 이용과 발현정도의 변화

김윤아 ( Kim Yun A ),이찬 ( Lee Chan ),김승조 ( Kim Seung Jo ),이선영 ( Lee Seon Yeong ),김인호 ( Kim In Ho ),나영정 ( Na Yeong Jeong ),정상근 ( Jeong Sang Geun ) 대한산부인과학회 2004 Obstetrics & Gynecology Science Vol.47 No.3

목적 : 난소암의 발암기전과 Insulin-like growth factor-2 (IGF-2), 그리고 H19 gene의 imprinting 상태, promoter 이용과 발현정도와의 연관성을 알아보기 위해, 본 연구는 8예의 정상난소조직, 11예의 양성난소종양, 9예의 경계성 종양과 20예의 악성종양 조직을 대상으로 실험하였다. 연구 방법 : 등록된 조직의 IGF-2와 H19을 PCR-RFLP 방법을 사용하여 이형접합성 분석을 하였으며, IGF-2의 promoter 특이발현을 살펴보고자 RT-PCR을 사용하였다. 결과 : 각 조직에서 IGF-2의 LOI (loss of imprinting)은 각각 정상 난소조직 (50%)> 양성종양 (60%) > 난소암조직 (71%) > 경계성 종양 (77%) 순으로 나타났다. H19 gene의 LOI는 정상과 양성종양의 난소조직에서는 관찰되지 않았고, 경계성 종양에서 100% (3/3), 악성종양에서 40% (2/5)로 나타났다. 각각의 난소조직에서 promoter P1, P2, P3와 P4의 이용도는 서로 다른 양상을 보였다. 암조직에서는 상대적으로 promoter P1, P2 이용이 높았으나 promoter P3 이용은 낮았다. 난소암조직에서 IGF-2의 발현은 높게 나타났으나 H19의 발현은 정상조직에 비해 낮음을 보였다. 결론 : 이상의 결과들을 보면 IGF-2의 LOI, promoter 사용의 deregulation IGF-2와 H19 발현정도의 변화가 난소암의 발생과 진행에 연관이 있으리라 생각된다. Objective : To establish the possible role of imprinting in ovarian cancer, we determined the imprinting status of both IGF-2 and H-19 genes in ovarian cancer, borderline tumors of ovary, benign ovarian tumor and normal ovarian tissues. Methods : An allelictyping assay was performed using a PCR-RFLP-based method for identification of heterozygous informative cases. The usage of Insulin-like growth factor-Ⅱ (IGF-2) promoters was examined by RT-PCR using promoter-specific primers. The mRNA expression of IGF-2 and H19 was quantified using a densitometer. Results : Loss of imprinting (LOI) of IGF-2 was observed in the order of borderline tumor (77%)>cancer (71%)>benign tumor (60%)>normal ovarian tissues (50%) respectively. And the LOI of H19 gene was not detected in the normal and benign tissues but observed in the borderline tumor and cancer tissues, respectively. The usage of promoter P1, P2, P3 and P4 were observed different pattern in normal, benign tumor, borderline tumor and cancer tissues. The activity of mRNA expression of promoter P4 was higher than other promoters. The cancer tissues predominantly used promoter P1, P2 with relative silencing of the promoter P3. The ovarian cancer tissues showed the higher expression levels of the IGF-2 but a down-regulation of the H19 relative to normal tissues. Conclusion : These results suggest that LOI, deregulation of the IGF-2 promoters, and the altered expression levels of the IGF-2 and h19 gene might be associated with progression of ovarian cancer.

Cannabidiol-induced apoptosis is mediated by activation of Noxa in human colorectal cancer cells

Jeong, Soyeon,Yun, Hye Kyeong,Jeong, Yoon A,Jo, Min Jee,Kang, Sang Hee,Kim, Jung Lim,Kim, Dae Yeong,Park, Seong Hye,Kim, Bo Ram,Na, Yoo Jin,Lee, Sun Il,Kim, Han Do,Kim, Dae Hyun,Oh, Sang Cheul,Lee, Da Elsevier 2019 Cancer letters Vol.447 No.-

<P><B>Abstract</B></P> <P>Cannabidiol (CBD), one of the compounds present in the marijuana plant, has anti-tumor properties, but its mechanism is not well known. This study aimed to evaluate the apoptotic action of CBD in colorectal cancer (CRC) cells, and focused on its effects on the novel pro-apoptotic Noxa-reactive oxygen species (ROS) signaling pathway. CBD experiments were performed using the CRC cell lines HCT116 and DLD-1. CBD induced apoptosis by regulating many pro- and anti-apoptotic proteins, of which Noxa showed significantly higher expression. To understand the relationship between Noxa and CBD-induced apoptosis, Noxa levels were downregulated using siRNA, and the expression of apoptosis markers decreased. After ROS production was blocked, the level of Noxa also decreased, suggesting that ROS is involved in the regulation of Noxa, which along with ROS is a well-known pro-apoptotic signaling agents. As a result, CBD induced apoptosis in a Noxa-and-ROS-dependent manner. Taken together, the results obtained in this study re-demonstrated the effects of CBD treatment <I>in vivo</I>, thus confirming its role as a novel, reliable anticancer drug.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Our results strongly suggest, for the first time, that CBD can cause Noxa-induced cell death. </LI> <LI> CBD induced apoptotic cell death via ROS/Endoplasmic Reticulum stress-regulated Noxa activation in colorectal cancer cells. </LI> <LI> These results suggest that CBD has important implications for the potential treatment of human CRC. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

hcp-Mg 입자분산형 Mg-Zn-Ce계 비정질합금의 제조와 기계적 성질

Kim, Seong-Gyu,Park, Heung-Il,Kim, U-Yeol,Jo, Seong-Myeong,Kim, Yeong-Hwan,Inoue, A.,Masumoto, T. 한국재료학회 1994 한국재료학회지 Vol.4 No.8

Mg-Zn-Ce계 합금에서 비정질 단상 및 hcp-Mg입자분산형 비정질합금이 20-40%, Zn, 0-10%Ce과 5-20%Zn, 0-5%Ce 의 조성범위에서 각각 생성되었다. 초미세 hcp-Mg입자분산형 $Mg_{85}Zn_{12}Ce_{3}$비정질합금은 급속응고 또는 급속응고리본의 열처리에 의해 Mg입자의 입경을 4-20nm의 범위로 조절할 수 있었으며, 이 범위에서는 밀착굽힘이 가능할 만큼 충분한 인성을 가지고 있었다. 이 합금의 최대인장강도($\sigma_{B}$)와 파단 연신율($\varepsilon_{f}$)은 hcp-Mg입자의 체적분율에 따라서 670-930MPa, 5.2-2.0%의 범위였으며, 최대 비강도($\sigma_{B}$밀도 =$\sigma_{s}$)는 $3.6 \times 10^5N \cdot m/kg$에 달하였다. 이와 같이 Mg입자분산형 비정질 합금의($\sigma_{B}$), ($\sigma_{s}$)그리고 $\varepsilon_{f}$의 최대치가 Mg-Zn-Ce계 비정질합금(690MPa, $2.5 \times 10^5N \cdot m/kg$, 2.5%)보다 월등하게 높다는 것은 주목할 만 하다. 복합상 조직이 형성됨으로서 기계적 강도가 증가하는 것은 동일 조성의 비정질상보다 강한 hcp과포화 고용체의 분산강화에 기인하는 것이라고 고찰되었다. An amorphous single phase and coexistent amorphous and hcp-Mg phases in Mg-Zn-Ce system were found to form in the composition ranges of 20 to 40% Zn, 0 to 10% Ce and 5 to 20% Zn, 0 to 5% Ce, respectively. A $Mg_{85}Zn_{12}Ce_{3}$ amorphous alloy containing nanoscale hcp-Mg particles was found to form either by melt spinning or by heat treatment of melt -spun ribbon. The particle size of the hcp-Mg phase can be controlled in the range of 4 to 20 nm. The mixed phase alloy prepared thus has a good bending ductility and exhibits high ultimate tensile strength($\sigma_{B}$) ranging from 670 to 930 MPa and fracture elongation($\varepsilon_{f}$) of 5.2 to 2.0%. The highest specific strength($\sigma_{B}$/density =$\sigma_{s}$)$3.6 \times 10^5N \cdot m/kg$. It should be noted that the highest values of flB, US and ?1 are considerably higher than those (690MPa,$2.5 \times 10^5N \cdot m/kg$and 2.5%) for amorphous Mg-Zn-Ce alloys. The increase of the mechanical strengths by the formation of the mixed phase structure is presumably due to a dispersion hardening of the hcp supersaturated solution which has the hardness higher than that of the amorphous phase with the same composition.