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최고야 ( Go Ya Choi ),고병섭 ( Byoung Seob Ko ),이미영 ( Mi Young Lee ),채성욱 ( Sung Wook Chae ),김영화 ( Young Hwa Kim ),육진아 ( Jin Ah Ryuk ),백지성 ( Ji Seong Baek ),이혜원 ( Hye-won Lee ) 대한본초학회 2011 大韓本草學會誌 Vol.26 No.1
Objective: This study was to compare antioxidant activity and HPLC pattern analysis from 4 species of changpo(changpu). Methods: To compare the antioxidant activity and HPLC pattern analysis from the 4 species of changpo, we performed the in vitro anti-oxidative activity assays and HPLC analysis from 70% ethanol extracts of Acorus gramineus Sol. (=AG), A. tatarinowii Schott (=AT), A. calamus L. (=AC) and Anemone altaica Fisch. ex C.A.Mey (=AA) taken in the herbal medicine market of Korea. Results: AG has the most effective anti-oxidative activity among 4 species of changpo. As the HPLC pattern analysis, AT was detected the unknown peak at retention time 14.9 min whereas AG was not showed any peak at the same retention time. These results suggest that AG could be used rather than AT when it need to be prescribed as anti-oxidative medicine. Conclusions: This result can be used as the basic data contributing to the stability of AG according to an appropriate clinical application.
미각센서를 이용한 중국산 감초와 우즈베키스탄산 광과감초의 감별
최고야 ( Go Ya Choi ),김영화 ( Young Hwa Kim ),채성욱 ( Sung Wook Chae ),이혜원 ( Hye Won Lee ),고병섭 ( Byoung Seob Ko ),이미영 ( Mi Young Lee ) 대한본초학회 2011 大韓本草學會誌 Vol.26 No.1
Objectives: Genetic analysis and taste pattern were performed to identify species between Glycyrrhiza uralensis and G. glabra which are officially listed in Korean Pharmacopoeia Ⅸ as origin of Gamcho(gancao, licorice root, Glycyrrhizae Radix et Rhizoma). Methods: Genetic analysis showed that identification between two species was done by comparing base sequence of ITS(intergenic transcribed spacer) and trnH-psbA regions from eleven Gamchoes sold in market. There was different taste pattern using by taste sensor in Glycyrrhiza uralensis and G. glabra. Results: Genetic analysis showed that six Gamchoes from China were identified as Glycyrrhiza uralensis and five Gamchoes from Uzbekistan were G. glabra. From the results of taste pattern, sourness and astringency of Glycyrrhiza uralensis from China were significantly higher than G. glabra from Uzbekistan, and aftertaste of astringency, aftertaste of umami, and saltiness of Glycyrrhiza uralensis were signicantly low as compared to G. glabra. There is no significant difference between two species in terms of bitterness, aftertaste of bitterness, and umami. Conclusions: Taken together, Glycyrrhiza uralensis from China and G. glabra from Uzbekistan were identified by taste sensor, and this technic could be applied to establishment of taste pattern marker for identification of different species located in various regions.
최고야 ( Go Ya Choi ),고병섭 ( Byoung Seob Ko ),장설 ( Seol Jang ),채성욱 ( Sung Uk Chae ),이혜원 ( Hye Won Lee ),이미영 ( Mi Young Lee ) 대한본초학회 2009 大韓本草學會誌 Vol.24 No.4
Objectives: Quality control for imported medicinal herbs is duty for regulation, however to the best of our knowledge there has been no report regarding quality control of imported ones. Therefore, this study investigated the data of imported ones for monitoring quality issue. Methods: These data are categorized according to item, the amount of import, country, inspection item, suitability, and incongruity reason based on the 248 results performed by KIOM. Results: China is the number one country for import with the value of 73%. Inspected medicinal herbs were 139 species, among them Poria is ranked as top for requested number and the amount of import. The amount of import is similar between high ranking 20 species and low ranking 119 ones, showing high ranking ones are major. The incongruity ratio was 7.7% from the total number of inspected items, the reason for incongruity was excess of tolerable amount of Cd residue. Conclusions: From the data, it is effective way to focus on high ranking herbs for evaluating imported herbs for quality control. The study of mechanism on Cd accumulation and its decrease is essential for environmental matter. And identification and storage for mixed used and easily decomposable herbs are remained for further study. Our study might be small step for showing guideline for proper quality control.
최고야 ( Go Ya Choi ),최은정 ( Eun Joung Choi ) 한국간호과학회 정신간호학회(구 대한간호학회정신간호학회) 2015 정신간호학회지 Vol.24 No.2
Purpose: This study is done to investigate the attitudes of teachers toward mental illness and to provide appropriate information about mental illness. Methods: The participants were 228 teachers recruited from 15 elementary schools in the National Capital region and B metropolitan city. Data were collected from October to December, 2013 and analyzed using t-test, ANOVA and Scheffe test with SPSS 18.0. Results: Neutral attitudes (77.4±7.79) toward mental illness were shown among the teachers. A negative attitude for stereotyping (23.2±6.08), a positive attitude for optimism (19.3±3.01) and neutral attitudes for coping (19.3±1.92) and understanding (15.6 ±3.05) were revealed. Conclusion: The results indicate that elementary school teachers have neutral attitudes towards mental illness which is closely related to the experience of learning about mental illness. Thus, when teachers learn about mental illness they can understand and guide children who have unusual behaviors.
동북아 5개국 공정서의 식물성 한약재 기원종 비교: 同名異屬種(동명이속종)을 중심으로
최고야 ( Go Ya Choi ),강영민 ( Young Min Kang ),문병철 ( Byeong Cheol Moon ),김호경 ( Ho Kyoung Kim ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.5
Objectives: Herbal medicines were used a lot in the Northeast Asia, traditionally. However, the pharmacopoeia standards in South Korea, China, Japan, Taiwan, and North Korea including many other Asia are different and cause confusion. If the origins are not belonging to same genus, it should be careful to distinguish. In this study, herbal medicines in the pharmacopoeia were analyzed for different genus of origins in order to identify the disruptive items for each country. Methods: The scientific names of herbal medicines (plant based) was analyzed origins from Pharmacopoeias of Republic of Korea, People`s Republic of China, Japan, Taiwan, and Democratic People`s Republic of Korea. The origins specified differently were examined. Especially, the items which have different genus were analyzed in detail and confirmed for correct scientific name. Results: The analyzed herbal medicines in Pharmacopoeia were all 753 items. 320 items were in only one country`s Pharmacopoeia. 237 items were in more than two countries` Pharmacopoeia, but their origins were same on each other. The items which have different genus were 35 items. Conclusions: In general, species belonging to the same genus have similar ecological, morphological, and pharmacological activity. However, species with different genus may have different medicinal ingredients and pharmacological activity. Thus, the items which have same name but different genus are required to analyze for comparison of pharmacological activity. Also, other species belonging to the different genus should be used for different items.
2종(種) 대황(大黃)의 외부(外部) 및 내외부형태(內外部形態)
김자영,최고야,김홍준,주영승,Kim, Ja-Young,Choi, Go-Ya,Kim, Hong-Jun,Ju, Young-Sung 한국한의학연구원 2006 한국한의학연구원논문집 Vol.12 No.1
The Following is a list of specific external and internal morphology of Rheum palmatum L. and R. undulatum L. in Rhei Rhizoma. 1. For the original plants of Rhei Rhizoma in the literatures. 18 plants are reported. 2. The external characteristics of original plant : R. palmatum has a large palmate leaf, its petiole of radical leaf has a simple bristle and its flower is purple and pink color. Whereas R. undulatum has a medium ovate leaf, its petiole of radical leaf is purphish, its stem leaf surrounds a half of stem and its flower is yellowish white color. 3. The character of herbal states : a. diameter and size-- R. palmaum is bigger and heavier than R. undulatum. b. in section--R. palmatum is dark brown color, R. undulatum is bright brown color Fibrovascular bundle is arranged in circle shape in R. patmatum. R. undulatum shows a distingushed wheel shape. 4. The internal characteristics : R. palmatum has a large intercellular space of cork layer, its cambium is clear and a xylem medullary ray is comparatively tight, Whereas R. undulatum has a tight intercettular space of cork layer., its cambium is a few clear and xylem medullary ray is verty tight. As these results, additional study is required to distinguish herbs and their effect among many kinds of same genus-degree of relatedness.
부자(附子)와 초오(草烏)의 내외부형태(內外部形態)와 패턴분석연구
강준혁,최고야,김홍준,주영승,Kang, Gyun-Heok,Choi, Go-Ya,Kim, Hong-Jun,Ju, Young-Sung 한국한의학연구원 2006 한국한의학연구원논문집 Vol.12 No.1
The taxonomic list of specific features in external and internal shape and the pattern analysis of Aconitum carmichalei $D_{EBX}$ as the original plant of Aconiti Lateralis Radix Preparata and Aconitum cliiare Dc as the original plant of Aconiti Ciliare Tuber are as follows. 1. Aconitum carmichalei $D_{EBX}$ has tri-palmately parted leaves, petiole in lower leaves, and its ovary has short hair. Whereas Aconitum cliare Dc has $3{\sim}4$ parted leaves, long petiole, and its ovary has not hair. 2. Aconitum carmichalei $D_{EBX}$ has cylinder shape is relatively small in length and diameter, is greyish brown blacky brown in outer surface, greyish $white{\sim}dark$ gray in section. 3. According to the collection place, there is a remarkable difference in the physical shape of herbal states. Aconiti Lateralis Radix Preparate(medicated in Korea) is more transparent blacky brown color than Aconiti Lateralis Fadix Preparata(medicated in Chian). Also Black Aconi Radix(墨附片) has exodermis and White Aconi Radix(白附片) has not. 4. The internal characteristics entirely correspond to in internal shape described in the literatures, Only it is possible to discriminate between black Aconi Radix(墨附片) and White Aconi Radix(白附片) by the existence of cork layer. The classification between Aconiti Lateralis Radix Preparata and Aconiti Ciliare Tuber makes entirely Tuber makes entirely remarkable difference in the physical shape of cambium layer Namely, in shape of cambium layer the kinds of Aconiti lateralis Radix Prepala has horn-like shape and the kinds of Aconiti Ciliare Tuber has circle-like shape. 5. In the peak of examination substance in comparison to Rt of the index material diterpene alkaloid mesaconitine, aconitine, hypaconitine chromatogram Aconiti Ciliare Tuber is higher than in Aconiti Lateralis Radix Preparata This explain that the component changes after the process of medicine. 6. In the Content of mesaconitine, aconitine and hypaconitime Aconiti Ciliare Tuber is higher than Aconiti Lateralis Radix Preparata. 7. In Aconiti Lateralis Radix Preparata, aconitine, hypaconitine and mesaconitine each appears in Rf 0.46, 0.54, 0.32. But except Aconiti Ciliare Tuber the band does not appear. For the future, such results will be used as the basic source of additional research, and a far-reaching comparative study is needed to distinguish between many kinds of same genus-degree of relatedness.