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홍충만(Choong Man Hong),윤창용(Chang Yong Yoon),홍이진(I Jin Hong),안병우(Byung Woo Ahn),김대중(Dae Joong Kim),민홍기(Honggi Min),김철규(Chuel Kyu Kim) 한국예방수의학회 2000 예방수의학회지 Vol.24 No.4
관상동맥 확장제로 임상에서 응용하고 있는 Dipyridamole(DIP)은 in vitro에서 혈소판응집 억제와 항산화작용을 가지고 있다. 그리고 멜라토닌은 송과선에서 분비되는 호르몬으로 항산화작용이 있기 때문에, 특히 실험적으로 유발한 간독성에 대하여 예방 및 치료효과가 있는 것으로 알려져 있다. 본 실험은 사염화탄소로 급성 간독성을 유발한 랫드에서 항산화효과가 있는 DIP와 멜라토닌을 단독 및 병행으로 전처리하여 간독성 예방효과를 비교하여 보았다. 실험군은 무처치 대조군과 사염화탄소 투여군으로 나누었다. 그리고 사염화탄소 투여군은 용매대조군, DIP 전처리군(100mg/kg, 근육내), 멜라토닌 전처리군(100mg/체중 kg, 복강내) 및 DIP와 멜라토닌 병행 전처리군(단독투여 전처리군과 동일농도)으로 나누어 매일 1회씩 3일간 반복투여하였다. 4일째에 사염화탄소(0.7ml/체중 kg, 경구)를 단회 투여하여, 24시간 후에 부검하여 혈액과 간조직을 얻었다. 그런 후, 혈중 간독성 지표효소인 ALT, AST, ALP와 GGT, 혈중 Triglyceride와 Cholesterol과 간조직 중 지방과산화 산물인 malondialdehyde(MDA)를 측정하였다. 실험결과 사염화탄소 투여군은 무처치 대조군과 비교하여 간독성의 지표효소와 지방과산화의 지표인자인 MDA가 통계학적으로 유의성 있게(p<0.05, p<0.01) 증가하였다. 사염화탄소 투여군에서 DIP와 멜라토닌을 단독으로 전처리한 실험군이 용매대조군에 비하여 간독성 지표효소와 MDA를 유의성 있게(p<0.05) 감소시켰다. 또한 DIP와 멜라토닌을 병행하여 전처리한 실험군이 용매대조군에 비하여 지표효소와 MDA를 유의성있게(p<0.01) 감소시켰으며, DIP와 멜라토닌만을 전처리한 실험군과 비교해서도 통계학적 유의성(p<0.05)을 관찰할 수 있었다. 따라서 사염화탄소로 유발한 실험적 급성 간독성 모델에서 DIP와 멜라토닌이 협력적으로 작용하여 간독성을 예방하였음을 알 수 있었으며, 사염화탄소의 지방과산화 작용으로 유발한 간 독성의 예방기전은 DIP와 멜라토닌의 항산화작용과 밀접하게 관련이 있는 것으로 사료되었다. 이 실험결과는 DIP가 멜라토닌의 간독 성 예방 및 치료효과를 증진시킬 수 있는 좋은 방법으로 응용할 수 있음을 보여주며, 이를 위해서 향후 DIP와 멜라토닌에 의한 협력 간독성억제에 관한 구체적인 기전 연구를 해야 할 것이다. This study was performed to evaluate the cooperative protective effect of DIP plus melatonin pretreatment on target enzymes activities and lipid peroxidation in CCl₄-induced male SD rats. Simultaneous pretreatment of DIP and melatonin markedly restored ALT, AST and GGT activities as well as triglyceride in serum compared with CCl₄-treated control group. Those pretreatments decreased in lipid peroxidation in terms of MDA level. DIP and melatonin themselves have hepatoprotective effect but co-administration of those chemicals shows more better results for the effect. The results presented in this study suggest that co-administration of DIP and melatonin may protect the liver against CCl₄-induced hepatotoxicity. Finally, this additional information may be important for therapeutic usefulness of melatonin. However, interactions between DIP and melatonin remains to be elucidated.
Cyclopiazonic acid 및 aflatoxin B<sub>1</sub>이 토끼의 혈소판 응집 및 ATP 방출에 미치는 영향
홍충만,조명행,Hong, Choong-man,Cho, Myung-haing 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.4
Cyclopiazonic acid(CPA) known as stimulating the release of intracellular calcium, aflatoxin $B_1(AFB_1)$ causing gastrointestinal hemorrhage frequently were used as model toxic mycotoxins in these studies. First of all, the effects of various mycotoxins on the platelet aggregation response were determined. The effects of mycotoxins on the ATP release from platelet by aggregating factors were investigated. The results and conclusions obtained from these studies are : 1) CPA promoted ADP, collagen, thrombin, A.A. and PAF-induced rabbit platelet aggregation. $AFB_1$ inhibited collagen, A.A. and PAF-induced rabbit platelet aggregation only. 2) CPA increased both aggregation and disaggregation time, whereas $AFB_1$ decreased in a dose dependent manner. 3) CPA increased ADP, thrombin, A.A. and PAF-induced ATP release. $AFB_1$ increased A.A.-induced ATP release and decreased PAF-induced release in a dose dependent manner. In conclusion, CPA promoted platelet aggregation by the increase of ATP. Antiaggregating effects of AFB1 may be due to decreases of ATP. These data provide the basis for the future study of roles of ATP release in platelet aggregation.
Sambutoxin이 토끼의 혈소판 응집에 미치는 영향
홍충만,조명행,Hong, Choong-Man,Cho, Myung-Haeng 한국독성학회 1998 Toxicological Research Vol.14 No.3
Sambutoxin, a newly purified mycotoxin in Koea, caused hemorrhage in the stomach and intestine of rats. To elucidate the mechanism of hemorrhage, effects of sambutoxin on rabbit platelet aggregation were investigated. First of all, the effects of sambutoxin on the platelet aggregation response and ATP release from platelet by various appregating factors were investigated. And then the role of $Ca^{2+}$ on the platelet aggregation was investigated by flow cytometer. Finally, morphological effect of sambutoxin on platelet ultrastructure was examined by transmission electron microscope. Sambutoxin inhibited aggregation induced by ADP, collagen, thrombin, and arachidonic acid and decreased platelet activating factor-induced disaggregation time in a dose dependent manner. Sambutoxin also decreased thrombin and arachidonic acid-induced ATP release, but increased all factors induced $Ca^{2+}$ release. Sambutoxin showed severe ultrastructural changes of platelet such as appearance of disorganization debri of cellular organelle in intercellular space. Our results indicate that sambutoxin inhibitis rabbit platelet aggregation, and it may be party due to the decrease of ATP release. However, it is not clear whether the antiaggregating effect of sambutoxin is related to $Ca^{2+}$ increase.
Cyclopiazonic acid 및 aflatoxin B<sub>1</sub>이 토끼의 혈소판에서 arachidonic acid 대사, 칼슘 동원 및 초미세구조에 미치는 영향
홍충만,장동덕,조명행,Hong, Choong-man,Jang, Dong-deuk,Cho, Myung-haing 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.4
For better understanding the interrelationship of hemorrhage and aggregation mechanism, cyclopiazonic acid(CPA) known as promoting the aggregation of platelet, aflatoxin $B_1(AFB_1)$ inhibiting platelet aggregation were used as toxic mycotoxins in these studies. In order to investigate the potential role of prostaglandin metabolism on the platelet aggregation, a variety of prostaglandin metabolites such as $PGF_{2{\alpha}}$, $PGE_2$ and $TXB_2$ were measured in homogenized rabbit platelets by TLC and LSC. And the role of $Ca^{{+}{+}}$ on the platelet aggregation was investigated by flow cytometer. Finally, the morphological effects of mycotoxins on platelet were determined by transmission electron microscope. The results and conclusions obtained from these studies are: 1) CPA induced no changes but $AFB_1$ increased $PGE_2$ and $TXB_2$. 2) CPA promoted ADP, collagen, thrombin, A.A., and PAF-induced $Ca^{{+}{+}}$ release. $AFB_1$, however, decreased $Ca^{{+}{+}}$ level except collagen-induced $Ca^{{+}{+}}$ release. When the calcium blocker, verapamil, was used, CPA decreased thrombin-induced $Ca^{{+}{+}}$ release and increased collagen, ADP, PAF and A.A.-induced $Ca^{{+}{+}}$ release. $AFB_1$ in contrast decreased the all factors induced $Ca^{{+}{+}}$ release. 3) $AFB_1$ did not induce any ultrastructural changes except large vacuole formation in a few platelets. And CPA also did not induce any changes except moderate shape change, indicator of platelet activation. In conclusion, CPA promoted platelet aggregation by the increases of $Ca^{{+}{+}}$ release but had no changes in A.A. metabolites. Antiaggregating effects of $AFB_1$ may be due to decreases of $Ca^{{+}{+}}$ release and increases of $PGE_2$ and $PGF_{2{\alpha}}$ formation. These data provide the basis for the future study of mobilization and function of $Ca^{{+}{+}}$ in platelet aggregation.
Establishment of the National Standard for Prekallikrein Activator with a Collaborative Study
In Soo Shin(신인수),Jae Yeon Cho(조재연),Soon Nam Kim(김순남),Choong Man Hong(홍충만),Ki Hong Lee(이기홍),Ho Jung Oh(오호정),Si Hyung Yoo(유시형),Jae Hyun Lim(임재현),Seung Eun Choi(최승은),Colin Longstaff,Hong Ki Min(민홍기),Sue 한국생물공학회 2004 한국생물공학회 학술대회 Vol.2004 No.4
김선희 ( Sun Hee Kim ),김종환 ( Jong Hwan Kim ),박기대 ( Ki Dae Park ),최민정 ( Min Jung Choi ),권오석 ( Oh Seok Kwon ),오일웅 ( Il Ung Oh ),정승태 ( Seung Tae Chung ),홍성화 ( Seong Hwa Hong ),홍충만 ( Choong Man Hong ) 한국조직공학과 재생의학회 2010 조직공학과 재생의학 Vol.7 No.3
Adipose tissue-derived Stem Cells(ADSCs) are utilized as a useful resource in many cell-based therapies. The ADSCs are often cryopreserved for future use but some cryoprotectants may have harmful influence on the stem cell quality such as stemness or viability. In this study, we investigated the influence of cryoprotectants such as DMSO, FBS and disaccharides on cell quality. ADSCs were frozen with variable combinations of cryoprotectants. The cells were stored in liquid nitrogen for a given period. The cells were thawed and tested for viability with trypan blue, the proportion of ADSCs were determined by flow cytometry analysis and the multipotency of the cells was assessed by differentiation potential of the cells into osteocytes or adipocytes. In the preliminary study, FBS had no additional benefit on the cells and, thus, the use of cryopreservation solution with serum free and low concentration of DMSO(5%) is suitable for cryopreservation of ADSCs used for cell-based therapy. From these data, we studied cell viability, MSC phenotype and differentiation potential as parameter of stability assessment. In result, there was no significant changes of viability, phenotype and representative CD marker according to our freezing condition/duration and concentration or volume of final products.
유통 축·수산물 중 잔류벤질페니실린의 검출 및 모니터링
이효정(Hyo Jeong Lee),강영원(Young Won Kang),이수민(Soo Min Lee),안경아(Kyung A An),이륜경(Ryun Kyung Lee),서상철(Sang Cheol Seo),이주희(Ju Hee Lee),임무혁(Moo Hyeog Im),이정림(Jeong Rim Lee),홍충만(Choong Man Hong),장문익(Moon Ik Ch 한국식품과학회 2014 한국식품과학회지 Vol.46 No.3
국내 유통 중인 돼지고기, 소고기, 닭고기, 넙치 232건의 시료에 대해 벤질페니실린의 잔류량을 조사하기 위해 현행 식품공전 상 축·수산물 중 벤질페니실린 분석법에 대한 검증을 실시하고 모니터링을 수행하였다. 그 결과 벤질페니실린에 대한 분석법은 CODEX에서 요구하는 기준에 적합한 정밀성과 정확성을 보였다. 벤질페니실린의 잔류량 모니터링 결과 축산물 193건 중 11건, 수산물 39건 중 2건 총 13건이 검출되었다. 검출된 수준은 축·수산물 12건에서 0.001-0.004 mg/kg으로 잔류허용기준 이하로 검출되었으나, 축산물 중 국내산 돼지고기 1건에서 잔류허용기준을 초과한 0.134 mg/kg 수준으로 검출되어 해당 유관기관에 부적합 통보 및 사후관리를 요청하였다. 따라서 벤질페니실린 등 항생제는 다른 동물용 의약품에 비해 사용빈도가 많은 만큼 지속적으로 잔류모니터링과 관리가 필요할 것으로 판단된다. Penicillin and its salts, including the benzatine, procaine, and sodium salts, have been widely used in human and veterinary medicine. Owing to their low toxicity, they currently form the most important group of antibiotics. However, overdose and abuse of these antibiotics may lead to potential risk in human health. Therefore, this study was conducted to validate the analysis method established by the Korea Food Code in 2012 and to monitor the levels of benzylpenicillin residues in products with reference to the maximum residue level (MRL). Of the 232 product samples tested, benzylpenicillin was detected in 11 livestock products and 2 marine products. Benzylpenicillin concentrations were found to be lower than the MRL in 12 products; however, the concentration of benzylpenicillin was found to be greater than the MRL in 1 pork product. The limit of quantification (LOQ) for benzylpenicillin was found to be 0.001-0.002 mg/kg, with an average recovery of 90.4-115.3%. Calibrations showed good linearity of 0.995 over a range of 0.002-0.05 mg/kg.