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      • KCI등재
      • KCI등재

        마이코박테리움을 신속하게 배양할 수 있는 성장 인자에 관한 연구

        하성일,박강균,석현수,신정섭,신동필,권민오,박연준,Ha, Sung-Il,Park, Kang-Gyun,Suk, Hyun-Soo,Shin, Jeong-Seob,Shin, Dong-Pil,Kwon, Min-O,Park, Yeon-Joon 대한임상검사과학회 2019 대한임상검사과학회지(KJCLS) Vol.51 No.2

        Mycobacterium은 느리게 성장한다. 따라서 고체배지는 8주, 액체배지는 6주 동안 사용하여야 한다. 이 연구의 목적은 Mycobacterium을 빠르게 성장시킬 수 있는 성장 인자를 찾고, 신속한 동정을 위한 고체배지를 개발하는 데 도움을 주는 것이다. $Difco^{TM}$ Mycobacteria 7H11 agar (Becton, Dickinson and Company)에 activated charcoal, defibrinated sheep blood, L-ascorbic acid를 첨가하여 10종의 Mycobacteria 가지고 Mycobacterium 성장 인자 3가지를 평가하였다. 집락의 검출 시간 및 판독 용이성을 현재의 방법과 비교하였다. 빠르게 성장하는 Mycobacterium 있어 새로운 배지와 기존의 배지에서 검출 시간의 차이는 새로운 배지가 더 빠르다는 것을 확인시켜 주었다. M. kansasii와 M. intracelluare는 7H11 배지보다 7H11 C 배지에서 더 빠르게 자라는 것으로 확인되었다. MTB는 7H11 C 배지에서 다른 배지보다 빠르게 성장하였다. 이 연구는 2 두 가지 성장 인자가 빠르게 성장하는 Mycobacteria과 느리게 성장하는 Mycobacteria에 영향을 주는 것으로 확인되었다. 7H11 C 배지는 색상 대비로 인하여 10종의 모든 Mycobacterium에서 기존배지보다 더 뛰어난 판독 용이성을 보여 주었다. 특히, MTB가 성장했을 경우 집락의 크기가 다른 배지에서 보다 커서 시각화가 용이하였다. Mycobacteria grow slowly. Therefore, a solid medium should be used for eight weeks and a liquid medium for six weeks. The purpose of this study was to find the growth factors that can grow Mycobacterium rapidly and to help develop a solid medium for rapid identification. Three types of Mycobacterium growth factors were evaluated with 10 Mycobacteria by adding activated charcoal, defibrinated sheep blood, and L-ascorbic acid to $Difco^{TM}$ Mycobacteria 7H11 agar (Becton, Dickinson and Company, Sparks, MD, USA). The time to detection and the distinguishability of a colony were compared with that of the current method. In the rapidly growing Mycobacterium, the difference in detection time between the new media and conventional media confirmed that the new media was faster. M. kansasii and M. intracelluare grew faster in 7H11 C than in 7H11 medium. MTB grew faster than the other media in 7H11 C. This study confirmed that the two growth factors affect fast-growing Mycobacteria and slow-growing Mycobacteria. 7H11 C showed better distinguishability than the conventional media in all 10 Mycobacterium due to the color contrast. In particular, when the MTB was grown, the size of the colonies was larger than with other media, so visualization was easy.

      • KCI등재

        CLSI M40-A2 기준에 따른 수송배지의 흡수 및 배출, 생존 효율, 회수율 평가

        하성일,석현수,신정섭,허웅,박강균,박연준,Ha, Sung-Il,Suk, Hyun-Soo,Shin, Jeong-Seob,Heo, Woong,Park, Kang-Gyun,Park, Yeon-Joon 대한임상검사과학회 2019 대한임상검사과학회지(KJCLS) Vol.51 No.2

        5종류의 수송배지를 흡수 및 배출 능력, 생존 효율, 회수율을 CLSI (Clinical and Laboratory Standards Institute) M40-A2의 Swab elution method (Quantitative) 기준으로 평가하였다. 액체배지가 반유동 배지보다 3가지 평가에서 대부분 우수한 결과값을 보여주었다. Flocked swab이 standard swab 형태보다 균의 흡수 및 배출의 능력 또한 우수하였다. 생존 효율에 대한 평가 결과는 액체배지(S4)가 가장 우수한 결과값을 보였다. 성장이 좋지 않은 S. pneumoniae는 액체배지(S4, S5)에서 생존효율과 회수율이 높았다. 균 회수율 평가 결과는 S. pyogenes는 모든 배지에서 CLSI 기준에 적합하였다. S. pneumoniae는 반유동 배지(S2, S3)에서 부적합하였고, 나머지 배지는 모두 기준에 적합하였다. H. influenzae는 반유동 배지(S1, S3)에서 부적합하였고, 반유동 배지(S2), 액체배지(S4, S5)에서 기준에 적합하였다. 호흡기 질환을 유발하는 S. pneumonia, H. influenzae의 생존 능력은 대부분의 배지에서 좋지 않았다. P. aeruginosa는 실온에서 과성장이 관찰되었다. 액체배지와 flocked swab의 조합이 3가지 평가 방법에서 가장 뛰어난 결과를 평가를 통해 확인하였다. The absorption and release capacities, survival efficiency, and recovery rates of five kinds of transport media were evaluated based on the swab elution method (Quantitative) of CLSI (Clinical and Laboratory Standards Institute) M40-A2. Liquid media showed mostly better results than semi-solid media in the three evaluations. The flocked swabs had better ability to absorption and discharge bacteria than the standard swabs. The liquid medium (S4) had the best survival efficiency. Pneumococcal strains with poor growth had a higher survival efficiency and recovery rate in liquid media (S4, S5). The results of microbial recovery showed that S. pyogenes met all the CLSI standards in all media. S. pneumoniae was inadequate in the semi- solid media (S2, S3) and all the remaining media met the criteria. H. influenzae was unsuitable in semi-solid media (S1, S3) and met the criteria in semi-solid medium (S2) and liquid medium (S4, S5). The viability of the H. influenzae, pneumococcal strain causing respiratory disease, was poor in most media. Overgrowth of P. aeruginosa was observed at room temperature. The combination of liquid medium and flocked swab confirmed the best results in the three evaluation methods.

      • SCOPUSSCIEKCI등재

        천골부에 발생한 거대 신경초종 - 증 례 보 고 -

        오경섭,하성일,이현성,이종수,곽승수,윤석훈,Oh, Kyung Seup,Ha, Sung Il,Lee, Hyun Sung,Lee, Jong Soo,Kwak, Seung Su,Yun, Suk Hoon 대한신경외과학회 2001 Journal of Korean neurosurgical society Vol.30 No.4

        Sacral schwannoma is a rare lesion with a tendency to reach large proportions. The benign schwannoma rarely involves the vertebral bodies extensively. The authors report a case of giant intrasacral schwannoma in 30-year-old woman who had intermittent lower back pain during 3 years period. CT and MRI showed a destructive mass lesion within the upper part of sacrum with a large mass extending into the presacral space. The patient underwent combined surgery consisted of anterior transabdominal approach and posterior sacral laminectomy and total removal of tumor. The characteristics of the lesion were discussed with a review of literatures.

      • SCOPUSSCIEKCI등재

        미만성 뇌축삭손상 환자의 자기공명영상 소견과 예후와의 상관관계

        오경섭,하성일,서범석,이현성,이종수,Oh, Kyung Seup,Ha, Sung Il,Suh, Bumn Suk,Lee, Hyun Sung,Lee, Jong Soo 대한신경외과학회 2001 Journal of Korean neurosurgical society Vol.30 No.1

        Object : We intended to investigate the relationship between the degree of injury on MRI and the outcome of the patients with diffuse axonal inury. Method : From january, 1995 to march, 1999, 22 patients were supposed to have diffuse axonal injuries by means of their neurologic signs and MRI. We investigated their prognosis according to CT, MRI and initial neurologic findings. Result : 1) The lesions were mainly located at white matter of cerebrum, corpus callosum, brainstem, and basal ganglia. 2) The lesions of white matter were most commonly in the frontal lobe and temporal lobe. 3) The majority of corpus callosal lesions were located in the posterior body and splenium, but anterior corpus callosal lesions combined with posterior lesions were not found. 4) Brainstem lesions, all non-hemorrhagic, were mostly located in the dorsolateral aspect, not be found on CT. 5) The brainstem lesions were found in 10 cases among total 22 cases, and corpus callosal lesions were accompanied with 8 cases of brainstem lesions. 6) The patients with brainstem lesions had worse prognosis. Conclusion : It is important and reasonable to take brain MRI to identify the brainstem lesions in any cases of suspicious diffuse axonal injury, and we should remind that the diffuse axonal injury with stem lesion has worse prognosis.

      • SCOPUSSCIEKCI등재

        터키안 주위부 연골종을 동반한 Maffucci 증후군 - 증례보고 -

        오경섭,서범석,하성일,이현성,이종수,윤석훈,Oh, Kyung Seop,Suh, Bumn Suk,Ha, Sung Il,Lee, Hyun Sung,Lee, Jong Soo,Yun, Suk Hoon 대한신경외과학회 2001 Journal of Korean neurosurgical society Vol.30 No.1

        Maffucci's syndrome is generally characterized by chondromatosis with vascular lesions of mesodermal origin, however, intracranial chondroma is rare. We present a case of Maffucci syndrome with paracellar chondroma and the review of literatures.

      • KCI등재

        방과 후 축구교실 참여 학생의 과제목표성향과 우리성 및 친사회적 행동의 관계

        이성철(Seong-Cheol Lee),하성일(Sung-Il Ha) 한국엔터테인먼트산업학회 2013 한국엔터테인먼트산업학회논문지 Vol.7 No.3

        이 연구는 방과 후 축구교실 참여 학생의 과제목표성향과 우리성 및 친사회적 행동의 관계를 규명하기 위하여 수도권에 소재한 학교 및 축구클럽에서 방과 후 축구교실에 참여하는 초등학생 및 중학생을 모집단으로 설정하였다. 유층집락 표집방법을 이용하여 4개 지역으로 나누어 각각 100명씩 총 400명의 표본을 수집하였으며 분석에 이용된 최종 표본 수는 269명이다. SPSS를 이용한 중다회귀분석 및 경로분석을 통하여 얻은 결론은 다음과 같다. 첫째, 과제목표성향은 우리성에 영향을 미친다. 둘째, 과제목표성향은 친사회적 행동에 영향을 미친다. 셋째, 우리성은 친사회적 행동에 영향을 미친다. 넷째, 과제목표성향은 우리성을 매개로 하여 친사회적 행동과 인과적 관계를 형성한다. The purpose of this study was to examine relationship between the task goal orientation and We-ness, pro-social behaviors among Korean students to participate in After-School Soccer Program. In order to accomplish this study"s purpose, this study surveyed 400 subjects who are elementary school and middle school students to participate in After-School Soccer Program. in metropolitan area. Questionnaire was filled out by self-administered method and data, valid 269 subjects, was analyzed by SPSS program. Statistics used in this study were descriptive analysis, analysis of variance, correlation, regressions, and path analysis. The followings are the results of this study: First, task goal orientation of students to participate in After-School Soccer Program affects We-ness. Second, task goal orientation of students to participate in After-School Soccer Program affects and pro-social behaviors. Third, We-ness affects pro-social behaviors. Fourth, the relationship between the task goal orientation of students to participate in After-School Soccer and We-ness, We-ness and pro-social behaviors were revealed.

      • KCI등재후보

        골프운동참가자의 골프운동중독성향과 Big-5 성격특성과의 관계

        김형석(Hyung Suk Kim),하성일(Sung Il Ha),김승준(Seung Joon Kim),유인영(In Young You) 한국체육교육학회 2010 한국체육교육학회지 Vol.15 No.3

        본 연구는 골프운동참여자를 대상으로 골프운동중독과 Big-5 성격요인과의 관계를 규명하는데 목적이 있다. 이를 위해 사용된 통계기법은 SPSS 12.0을 이용한 빈도분석, 신뢰도분석, 요인분석, 상관관계분석, 일원변량분석, 다중회귀분석 등이 사용되었으며, 이와 같은 절차를 통해 다음과 같은 결론을 도출하였다. 첫째, 골프운동참여자의 골프운동중독성향의 차이를 검증한 결과 골프운동중독성향은 남자가 여자에 비해 높게 나타났고, 연령에 따라서는 40대가, 경력에 따라서는 5년 이상 된 집단이 다른 집단에 비해 골프운동중독성향이 높은 것으로 나타났다. 둘째, 골프운동참여자의 Big-5성격특성 요인 중 성실성은 골프운동중독 요인 중 금단현상, 운동욕구에 정(+)적으로 유의한 영향을 미치는 것으로 나타났다. 셋째, 골프운동참여자의 Big-5성격특성 요인 중 정서적 불안정성은 골프운동중독 요인 중 운동욕구, 사회적 적응, 감정적 애착에 정(+)적으로 유의한 영향을 미치는 것으로 나타났다. 넷째, 골프운동참여자의 Big-5성격특성 요인 중 호감성은 골프운동중독 요인 중 운동욕구, 사회적 적응에 정(+)적으로 유의한 영향을 미치는 것으로 나타났다. 다섯째, 골프운동참여자의 Big-5성격특성 요인 중 지적 개방성은 골프운동중독 요인 중 운동욕구, 감정적 애착에 정(+)적으로 유의한 영향을 미치는 것으로 나타났다. This study aims to find out the relationship between the addiction to golf exercise and the Big-5 personality factors for golf exercise participants. The statistical techniques used for this were reliability analysis, factor analysis, correlation analysis, one-way ANOVA, multiple regression analysis, and frequency analysis using SPSS 12.0, and the following results were drawn through such procedures: First, according to the verification of the difference of golf exercise addition between golf exercise participants, golf exercise of male is relatively higher than one of female. And people in their 40s by age, and the group with more than 5 years by their career show higher addiction to golf exercise compared to other groups. Second, sincerity among Big-5 personality factors of golf exercise participants gives significant positive effect on withdrawal, and exercise needs out of golf exercise addiction factors. Third, emotional lability among golf exercise participants` Big-5 personality factors gives significant positive effect on exercise needs, social adjustment, and emotional attachment out of golf exercise addiction factors. Next, agreeable impression among Big-5 personality factors of golf exercise participants gives significant positive effect on exercise needs, and social adjustment out of golf exercise addiction factors. Fifth, intellectual openness among Big-5 personality factors of golf exercise participants has significant positive effect on exercise needs and emotional attachment among golf exercise addiction factors. Based on the above results future researches need to find out social phychological factors related to golf exercise addiction in a various way, and more specific and empirical researches including longitudinal qualitative research according to quantitative study results are required. And acquisition of more sound golf exercise habits will be necessary by learning and understanding the knowledge on golf exercise addiction.

      • KCI등재

        골프클럽소비자 성격의 Big 5요인과 브랜드 애착 및 구매의도의 관계

        김형석(Kim Hyung-Suk),하성일(Ha Sung-Il) 한국체육과학회 2010 한국체육과학회지 Vol.19 No.2

        This study examined gathered data from 400 golf club users in 15 golf courses and practice ranges located in Seoul, Gyeonggi-Do and Daejeon area to examine the impact of 5 personality factors on golf club user's brand attachment and purchase intention. Among them, incomplete data from 36 users were excluded and total 363 were analyzed in this study. The data was submitted to SPSS 12.0 and AMOS 6.0 for explorative factor analysis, confirmative factor analysis and structural equation modeling. The results are as follows. First, the emotional unstability among the 5 personality factors of golf club users has a positive impact on the brand attachment. Second, the extraversion factor has a positive impact on the brand attachment. Third, the sincerity factor has a positive impact on the brand attachment. Fourth, the brand attachment of golf club users has a positive impact on purchase intention. These results suggest that the manager or seller of the golf club brand needs to understand that the emotional unstability, extraversion and sincerity factor may be the direct cause for the brand attachment and the brand attachment for the purchase intention and needs to establish reliable product sales and responsible after-sales management as well as various marketing strategies.

      • KCI등재

        Strength Band Training 프로그램이 대학농구선수들의 민첩성과 순발력에 미치는 영향

        하성(Ha, Sung),서대성(Seo, Dae-Seong),강두찬(Kang, Doo-Chan),서동일(Seo, Dong-Il) 한국체육과학회 2012 한국체육과학회지 Vol.21 No.6

        The purpose of this study was to investigate that the effects of a strength band training(SBT) program on agility and power in college basketball players. 14 college basketball players were participated in this study. They did the SBT program (Start and 3 steps, Hurdle running, Side hurdle jump, "∞" Drill, Shuttle with hurdle, Back pedal 3 steps, Turn and go 3 steps, Box jump, Box lunge jump) 15 repetition/15 sets of each and 3 times per week for 3 weeks. They were tested Sargent jump, 10m Sprint test, Agility T test 3 Cone shuttle drill test, Illinois agility test, Zig Zag run test, and Side step test. There was a significant improvement on all tests for agility and power(p<.05). In conclusion, this study indicate that the SBT program is an effective training method to improve their agility and power for college basketball players.

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