In Vitro 자계(磁界) 측정에 의한 비소화합물의 폐포 Macrophage 독성 평가

조영채,Cho, Young-Chae 대한예방의학회 1999 Journal of Preventive Medicine and Public Health Vol.32 No.4

Objectives: This study was conducted to evaluate the cytotoxicity of gallium arsenide(GaAs), indium phosphide(InP) and indium arsenide(InAs) all of which are used a$ the semiconductor eletments in semiconductor industry. Methods: Cytotoxicity id the alveolar macrophage was evaluated by the measurement of in vitro magnetometry, LDH release assay and histological examination. Results: The relaxation curves by the in vitro magnetometry showed that GaAs has the cytotoxicity for the alveolar macrophage which is more significant in the higher dosages, while this cytotoxicity is not appeared in the groups added with InP or InAs or PBS. In the decay constant for two minutes after magnetization, GaAs-added groups showed a significant decrease with increasing doses, but both InP- and InAs-added groups did not show any significance. The LDH release assay showed a dose-dependent increasing tendency in the GaAs-, InP- and InAs-added groups. In terms of cellular morphological changes, GaAs-added groups revealed such severe cellular damages as prominent destructions in cell membranes and their morphological changes of nucleus, while InP- and InAs-added groups remained intact in intracellular structures, except for cytoplasmic degenerations. Conclusions: It is suggested that GaAs is more influential to cytotoxicity of alveolar macrophages than InP and InAs. 본 연구는 반도체 산업에서 반도체소자로서 주목받고 있는 GaAs, InP및 InAs의 세포독성을 평가하기 위해 햄스터의 폐포 대식세포를 사용하여 in vitro 자계 측정, LDH 활성치측정 및 세포의 형태학적 관찰 등을 검토하였다. 세포자계측정 결과 GaAs, InP 및 InAs첨가군 모두 대조군(PBS첨가군)에 비해 완화곡선이 유의하게 지연되었으며, 특히 GaAs 첨가군은 농도증가에 따라 용량의존적으로 완화곡선이 지연되는 경향이었다. 자화 후 2분간의 완화계수는 대조군에 비해 GaAs 첨가군은 농도증가에 따라 유의하게 낮아지는 용량의존성이 높은 경향이었으나, InP 및 InAs 첨가군에서는 모두 유의성이 인정되지 않았다. LDH활성치는 GaAs, InP 및 InAs첨가군 모두 용량 의존적으로 점차 높아지는 경향이었다. 세포의 형태학적 관찰소견은 GaAs첨가군에서는 용량의존적으로 세포막의 현저한 파괴, 핵의 형태적 변화 등 심한 세포장해가 유발된 반면, InP첨가군과 InAs첨가군에서는 세포내의 구조는 유지되었으나 세포질의 변성이 관찰되었다. 결과적으로 GaAs는 InP나 InAs보다 폐포 대식세포의 세포독성이 강한 것으로 보인다.

청목노상(Morus alba cv. Cheongmoknosang) callus의 배양조건에 따른 Helicobacter pylori 억제물질의 생산

조영제(Young-Je Cho),차원섭(Won-Seup Cha),강선애(Sun-Ae Kang),안봉전(Bong-Jeun An),안동현(Dong-Hyun Ahn),김명욱(Myung-Uk Kim),채정우(Jung-Woo Chae) 한국생명과학회 2013 생명과학회지 Vol.23 No.3

Helicobacter pylori 억제효과가 우수한 청목노상의 캘러스 배양을 위한 최적조건은 MS 고체배지에서 27℃에서 20일간 배양하였을 때 6.4 mm의 크기로 가장 크게 자랐으며, 청목노상의 callus 형성에 미치는 생장조절제로는 NAA, 2,4-D, BA 및 kinetin 등을 2 mg/l의 농도로 첨가하여 27℃에서 20일간 배양했을 때 높은 캘러스 성장률을 확인 할 수 있었고, 생장조절제의 혼합처리구가 단독처리구 보다 캘러스 형성율이 높은 것을 확인 할 수 있다. 따라서 본 연구에 사용된 청목노상 품종의 평판기내 배양을 위한 direct callogenesis의 최적 조건은 생장호르몬으로 2,4-D/NAA를 2 mg/l의 농도로 혼합 처리하여 27℃에서 20일간 배양이 최적조건이었다. 청목노상 callus로부터 Helicobacter pylori 억제물질의 대량생산을 위한 방법인 biomass를 위한 bioreactor배양은 MS 액체배지에 호르몬으로 2,4-D와 BA를 각각 1 mg/l의 농도로 처리하여 20일간 배양하였을 때가 최적조건이었다. 최적조건에서 배양한 callus 추출물의 Helicobacter pylori 에 대한 억제효과는 16 mm의 clear zone으로 가장 높은 저해율을 확인할 수 있었다. The optimal condition for Morus alba cv was an MS culture medium at 27℃ for 20 days. Cheongmoknosang callus showed inhibitory activity against Helicobacter pylori at 1.05 g of wet weight of the cultured callus. The callus formation of Morus alba cv. Cheongmoknosang was influenced by naphthalene acetic acid (NAA), 2,4-dichlorophenoxy acetic acid (2,4-D), 6-benzylaminopurine (BA) and kinetin at concentrations of 2 mg/l. The growth rate of callus was higher than it was when these hormones were mixed with a single hormone. Thus, the optimal condition for direct callogenesis was to incubate with mixture (2,4-D/NAA) of 2 mg/l concentration at 27 ℃ for 20 days. Moreover, the optimal culture condition of the biomass in the mass production of inhibitory compounds against Helicobacter pylori from Morus alba cv. Cheongmoknosang callus was to incubate in an MS broth (each concentration 1 mg/l of 2,4-D and BA). When Morus alba cv. Cheongmoknosang callus were incubated for 20 days in a bioreactor, Helicobacter pylori inhibition of callus extracts was the highest at a clear zone of 16 mm.

일부 농촌지역 노인들의 낙상발생과 관련된 요인

조영채(Young-Chae Cho),윤현숙(Hyun-Suk Yoon) 한국농촌의학 지역보건학회 2004 농촌의학·지역보건 Vol.29 No.2

종합건강검진 수검자들의 대사증후군 유병률 및 관련요인

조영채(Cho, Young-Chae),권인선(Kwon, In-Sun),박재영(Park, Jae-Young),신민우(Shin, Min-Woo) 한국산학기술학회 2012 한국산학기술학회논문지 Vol.13 No.11

본 연구는 대사증후군 및 대사증후군 진단기준 인자의 유병률을 파악하고, 인구사회학적 및 건강관련행위 요인과의 관련성을 검토하며, 진단기준 인자의 군집화를 통해 대사증후군 위험의 분포를 분석하고자 하였다. 조사는 한 대학병원 건강검진센터에서 종합건강검진을 받았던 30세 이상의 지역주민 1,388명을 대상으로 허리둘레, 중성지 방, 고밀도지단백콜레스테롤, 수축기혈압, 확장기혈압, 및 공복 시 혈당 등 대사증후군 진단기준 인자를 측정하였다. 분석은 이들 대사증후군 위험인자에 대한 유병률을 파악하고 위험인자의 군집화를 통해 대사증후군 위험의 분포를 파악하였으며, 관련요인에 따른 대사증후군의 위험비를 구하였다. 연구결과 조사대상자의 대사증후군의 유병율은 21.7%로 나타났으며, 대사증후군 위험인자별 유병률은 HDL-C, 혈압, TG, 허리둘레, FBS의 순으로 나타났다. 또한 관련변수에 따른 위험비에서 BMI, 흡연습관, 식품섭취 및 당뇨에 대한 가족력 등이 대사증후군의 위험비를 높이는데 관련된 것으로 나타났다. 따라서 대사증후군의 위험 관리를 위해서는 모든 생활습관 요인에 대한 평가와 중재가 필 요함을 시사하고 있다. The purpose of this study was to estimate the prevalence of metabolic syndrome and determine the distribution of the clustering of the metabolic risk factors, and we wanted to evaluated the related factors in urban areas. 1,388 adults of 30 years and over, not recognized as taking medicines for or having cardiovascular diseases, who underwent health package check-up at the health promotion center of a university hospital. All subjects were measured by height, weight, waist circumference, blood pressure and blood chemistry(lipid profile). As a results, the prevalence rates of metabolic syndrome of study subjects were 21.7%, and the rates of metabolic risk factors were HDL-C, blood pressure, TG, abdominal obesity and FBS in that order. And the factors such influencing on metabolic syndrome as age, BMI, smoking habits, vegetable consumption and family history of the diabetes. Consequently, it is suggested that the evaluation and intervention for lifestyle factors may be needed in order to the risk management of metabolic syndrome.

정금나무(Vaccinium oldhami) 열매의 생리활성 및 항균활성

채정우(Jung-Woo Chae),조분성(Bun-Sung Jo),주성현(Sung-Hyun Joo),안동현(Dong-Hyun Ahn),천성숙(Sung-Sook Chun),조영제(Young-Je Cho) 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.1

정금나무 열매의 생리활성 및 항균활성을 측정한 결과, ABTS radical decolorlization은 200 μg/mL에 92.7±4.1%로 positive control인 BHA의 200 μg/mL에서 52.4±1.9%보다 높은 활성을 보였다. 지용성 물질의 항산화력을 나타내는 TBARS값은 200 μg/mL에서 74.4±2.9%, antioxidant protection factor는 200 μg/mL에서 3.6±1.6%로 항산화효과가 우수한 것으로 나타났다. 정금나무 열매 추출물의 angiotensin converting enzyme 활성억제 효과는 200 μg/mL에서 28.6±0.6%의 저해율을 나타내었으며, xanthine oxidase 저해활성을 측정한 결과 200 μg/mL에서 43.3±0.8% 이상의 활성을 나타내었다. 정금나무 열매 추출물의 항균활성을 측정한 결과 Helicobacter pylori는 200 μg/0.1 mL 농도에서 24 mm의 저해환을 보였고, Staphylococcus epidermidis는 28 mm의 저해환을, Staphylococcus aureus, Eschericia coil, Propionebacterium acnes는 각각 13 mm, 26 mm, 16mm의 저해환을 나타내어 200 μg/0.1 mL의 농도 이상에서는 모두 항균활성이 나타나는 것을 확인할 수 있었다. 주름억제 효과를 나타내는 elastase 억제효과를 측정한 결과 200μg/mL에서 52.7±0.9%로 비교적 높은 활성을 나타내었다. This study was carried out to investigate the biological activity and antimicrobial activity of Vaccinium oldhami fruit extracts. ABTS radical cation decolorization and the antioxidant protection factor (PF) of extracts as 92.7±4.1% and 3.6±1.6 PF were higher than a BHT of 200 μg/mL as 52.4±1.9% and 2.0±0.8 PF, and the TBARS of extracts was 74.4±2.9% with 200 μg/mL. The hypertension inhibitory activity of extracts from Vaccinium oldhami fruit indicated the activities of 28.6±0.6% with 200 μg/mL, and anti-gout activity was 43.3 ±0.8% with 200 μg/mL. Antimicrobial activity was found in Vaccinium oldhami fruit extracts on Helicobacter pylori, Staphylococcus epidermidis, Staphylococcus aureus, Eschericia coli and Propionebacterium acne. This activity was illustrated as 24 mm, 28 mm, 13 mm, 26 mm and 16 mm clear zones with 200 μg/mL respectively, and the elastase inhibitory activity which is related to the wrinkle cause was observed in extracts as 52.7±0.9% with 200 μg/mL.

Lactobacillus acidophilus 발효유가 cadmium투여된 흰쥐의 혈액상과 신장조직에 미치는 영향

조영채,전무형,장경수,Cho, Young-chae,Jun, Moo-hyung,Chang, Kyung-soo 대한수의학회 1994 大韓獸醫學會誌 Vol.34 No.4

To elucidate the protective effects of Lactobacillus acidophilus-fermented milk against cadmium toxicity, the effects of administration of L acidophilus-fermented milk on hematological values and histopathological changes in cadmium-treated rats were investigated. The experimental rats were divided into 2 groups that were consisted of the one group administered with cadmium alone, and the other group administered with cadmium mixed with the fermented milk. Each group was orally administered with different doses of cadmium such as $1.7{\mu}g/g$ bw/day, $3.4{\mu}g/g$ bw/day, $6.8{\mu}g/g$ bw/day, and $13.6{\mu}g/g$ bw/day, respectively, for 1 to 8 weeks. Hematological values and enzyme activities, histopathological changes of kidney tissues were examined for the experimental groups. The values of RBC, WBC, and Hb in the groups administered with cadmium mixed with the fermented milk showed no significant differences to those of the groups administered with cadmium alone, but Hct showed significant reducing values. The activities of glutamic oxaloacetic transaminase(GOT) and glutamic pyruvic transaminase (GPT) in serum were significantly reduced than those of the groups administered with cadmium alone, at the low dose of cadmium treated groups. But alkaline phophatase(ALP) and lactate dehydorgenase(LDH) were significantly reduced at the high dose of cadmium treated groups. In histopathological study, a severe acute tubular necrosis of the convoluted tubules and distalation of tubules were showed in the groups administered with cadmium alone, but the kidney tissues of the groups administered with cadmium mixed with the fermented milk were similar to those of the normal group. In conclusion, the above results would suggest that L acidophilus-fermented milk has reducing effects on cadmium toxicity, at the low dose of cadmium administration.

약물치료에 반응을 하지 않았던 비폐쇄성 연하곤란 1 예

조영석(Young Seok Cho),최명규(Myung Gyu Choi),정우철(Woo Chul Chung),정정조(Jeong Jo Jeong),이인석(In Seok Lee),김상우(Sang Woo Kim),김성수(Sung Soo Kim),채현석(Hiun Suk Chae),한석원(Sok Won Han),정인식(In Sik Chung),박두호(Doo Ho Pa 대한소화기기능성질환·운동학회 2002 Journal of Neurogastroenterology and Motility (JNM Vol.8 No.1

Dysphagia is a frequent symptom which is observed in about 6% of all population. The most common causes of nonobstructive dysphagia are reported to be esophageal motility disorders, systemic disease and GERD. About 30% of GERD patients complain of dysphagia. Dysphagia in GERD patients is mostly due to peptic stricture but occasionally related to transient segmental esophageal motor disorder. A 42-year old male patient was admitted because of dysphagia and weight loss. He had renal transplantation 5 years ago. The results of esophagogastroduodenoscopic examination, esophagogram and esophageal manometry were normal. Treatment with proton pump inhibitor, prokinetic and anti-depressant was begun but the patient continued to complain of dysphagia. The result of 24 hour ambulatory pH monitoring revealed pathologic reflux. The results of 24 hour ambulatory esophageal manometry and video fluoroscopy were normal. After reassured, he didn`t complain of dysphagia. We report this case of nonobstructive dysphagia accompanied with GERD refractory to medical treatment in patient who had renal transplantation.(Korean Journal of Gastrointestinal Motility 2002;8:47-52)

29 위점막에서 H. pylori 감염에 대한 human β-defensin-2의 역할

조영석 ( Young Seok Cho ),전은정 ( Eun Jung Jun ),김성수 ( Sung Soo Kim ),채현석 ( Hiun Suk Chae ),한석원 ( Suk Won Han ),이창돈 ( Chang Don Lee ),정인식 ( In Sik Chung ),박두호 ( Doo Ho Park ) 대한소화기학회 2002 대한소화기학회 추계학술대회 Vol.2002 No.-

산업장 근로자들의 연령과 건강습관에 따른 비만 , 혈압 및 혈청지질의 특성

조영채(Young Chae Cho),송인순(In Soon Song) 대한보건협회 2000 대한보건연구 Vol.26 No.2

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일부 대학생의 자가 판단에 의해 사용하는 일반의약품에 대한 지식, 인식태도 및 사용실태와 관련요인

조영채(Young-Chae Cho),양혜경(Hye-Kyeong Yang) 한국보건교육건강증진학회 2007 보건교육건강증진학회지 Vol.24 No.1