산사(Crataegi Fructus) 추출물로부터 ${\alpha}-amylase$와 ${\alpha}-glucosidase$ 저해 물질 분리 및 동정

김명욱,조영제,김정환,Kim, Myung-Uk,Cho, Young-Je,Kim, Jeung-Hoan 한국응용생명화학회 2007 Applied Biological Chemistry (Appl Biol Chem) Vol.50 No.3

산사 추출물로부터 ${\alpha}-amylase$, ${\alpha}-glucosidase$ 저해활성을 탐색하고 Sephadex LH-20($5{\times}45$ cm)와 MCI-gel CHP-20를 이용하여 저해활성 물질을 정제하고 구조분석을 하였다. 산사 추출물의 페놀화합물의 함량은 물 추출물에서 22.5 mg/g, 60% ethanol 추출물에서 19.2 mg/g으로 나타났으며, 산사 물 추출물 (200 ${\mu}g/ml$)에서 ${\alpha}-amylase$, ${\alpha}-glucosidase$ 저해활성이 각각 100%와 82.6%로 나타났으나, 60% ethanol 추출물(200 ${\mu}g/ml$)에서는 ${\alpha}-glucosidase$ 저해활성이 12%로 낮은 저해활성을 나타내어 산사 물 추출물을 Sephadex LH-20를 이용하여 분획한 결과 I, II, III, IV의 4가지 분획물을 획득하고 다시 Sephadex LH-20과 MCI-gel을 이용하여 nomal phase type인 $EtOH{\rightarrow}H_2O$와 reverse phase type인 $H_2O{\rightarrow}MeOH$로 유기용매의 농도를 증가 시키며 용출하여 A, B, C, D의 compounds를 얻었다. 4가지 compound 중 compound C(200 ${\mu}g/ml$)가 ${\alpha}$-amylase, ${\alpha}$-glucosidase 저해활성이 각각 97.6%, 97.7%로 가장 높게 나타났으며, FAB-MS, NMR과 IR spectrum을 분석한 결과 chlorogenic acid로 동정되었다. The objective of this research was to evaluate the inhibitory activities of phenolic compounds isolated from Crataegi Fructus on ${\alpha}-amylase$ and ${\alpha}-glucosidase$. The content of total phenolic compounds of water extract from Crataegi Fructus was 22.5 mg/g. The inhibitory activity of the water extract (200 ${\mu}g/ml$) from Crataegi Fructus on ${\alpha}-amylase$ and ${\alpha}-glucosidase$ was determined to be 100% and 82.6%, respectively. Isolation of inhibitory compounds was carried out on Sephadex LH-20 and MCI-gel CHP-20 column chromatography using a gradient elution procedure of increasing MeOH in $H_2O$. The chemical structure of the inhibitory compound against ${\alpha}-amylase$ and ${\alpha}-glucosidase$ was confirmed as chlorogenic acid by spectroscopic analysis of FAB-MS, NMR and IR spectrum.

Aloe vera L. Callus의 현탁배양에 의한 세포외 다당생산 및 최적화

김명욱 ( Myung Uk Kim ),조영제 ( Young Je Cho ),이신영 ( Shin Young Lee ) 한국산업식품공학회 2012 산업 식품공학 Vol.16 No.3

The extracellular polysaccharide production from suspension culture of Aloe vera L. was analyzed, and the optimization for suspension cultivation was investigated. From specific polysaccharide assay of glucomannan, FT-IR and 1H NMR spectrum, the extracellular polysacchride from suspension culture was found to be similar to β-1,4-glucomannan originated from Aloe vera fresh leaf. The polysaccharide existed in an acetylated form and its molecular weight by gel permeation chromatography was estimated to be 490 kD (Mn = 440 kD; polydispersity = 1.115). From medium optimization by simplex-cemtroid design using MINITAB®, the callus growth on the 2,4-D of 11.82 μM, kinetin of 13.84 μM and α-naphthalene acetic acid of 4.34 μM was turned out to be significantly superior than the growth on the other culture media combinations. Under this optimal culture media, other conditions such as the suspension culture``s temperature, pH and the growth terms were determined. These results showed that the callus growth was optimum at 25oC, pH 5.5 and in 2 weeks. The maximum levels of growth and extracellular polysaccharide production of callus by optimal conditions were about 20.4 and 2.5 g/L, respectively, showing the profile of extracellular polysaccharide production was closely related to the callus growth.

Aloe vera Callus 현탁배양 생성물의 항염증 활성

김명욱 ( Myung Uk Kim ),조영제 ( Young Je Cho ),이신영 ( Shin Young Lee ) 한국응용생명화학회(구 한국농화학회) 2013 Journal of Applied Biological Chemistry (J. Appl. Vol.56 No.3

알로에의 유효성분인 다당의 효율적 생산연구일환으로 수행되었던 aloe callus의 현탁배양과 관련하여, 본 연구에서는 이의 배양생성물(세포외 다당)과 callus의 용매추출물에 대한 항염증활성을 조사하였다. 세포외 다당(ACP)과 callus (AC)의 물 및에탄올 추출물의 대식세포(Raw 264.7) 세포생존율을 MTT assay로 조사한 결과, AC-DMSO의 400 μg/mL 첨가구에서 97%로 미미한 증식저해를 보였으나 나머지 시료처리구는 모두증식 촉진효과를 나타내었다. 또, LPS에 의해 활성화된 Raw 264.7 세포 배양액의 알로에 추출물 처리에 따른 NO 생성 저해효과를 조사한 결과, AC-DW와 AC-DMSO는 오히려 양성대조구인 LPS 단독보다 더 많은 NO를 생성하였다. 특히, 세포외다당 분획인 ACP-DW는 NO 생성을 강력히 억제하여 80-100μg/mL의 비교적 낮은 농도의 첨가로 대조구 수준까지 억제하였다. 또, Raw 264.7 세포에 LPS로 자극을 주고 알로에 현탁배양 생성물의 처리에 따른 COX-2단백질의 발현정도를 조사한 결과, ACP-DW 처리구는 단독으로 COX-2 단백질을 발현시키지 않았으며, LPS 자극에 따른 COX-2의 발현감소는 미미하였다. 아울러, 활성화된 대식세포로부터 생성된 염증 사이토카인인 IL-1β와 TNF-α의 발현량을 RT-PCR로 조사한 결과, ACPDW처리구에서 TNF-α의 발현이 강력하게 억제되었다. 이상의 결과들은 알로에 현탁배양 유래의 생성물들이 비교적우수한 항염증활성을 나타냄을 보여주는 결과로, 그동안 삽목번식의 재배방법에 의존하였던 알로에 유효성분의 생산을 대체할 수 있는 효율적이고, 새로운 방법으로서의 알로에 callus의 현탁배양 가능성을 보여주었다. Cultured products (callus and exopolysaccharide) were obtained from suspension culture of Aloe vera callus, and the extracts of callus were further prepared with cold water or 60% ethanol solution. The ethanol extract of callus (AC) and exopolysaccharide (ACP) of 10 mg/mL exhibited the relatively higher suppression activity of 43.2-52.1% against hyaluronidase activity. Thus, their anti-inflammatory effects were further investigated using animal cell (Raw 264.7) in vitro. Though AC shows a slight suppression effect of cell survival rate (97%) using MTT assay in the presence of 400 μg/mL AC- dimethyl sulfoxide (DMSO), cell growth promotion was observed in the other samples of lower levels. It indicates that the ethanol extract of Aloe callus rarely affect cell survival rate in the ranges (200-400 μg/mL) used in the study. Using Griess reagent, the suppression of NO production by the aloe callus extract was analyzed by measuring the amount of the nitrite produced in Raw 264.7 culture activated by lipopolysaccharide (LPS). As a result, supplementation of AC-distilled water (DW) and AC-DMSO produced higher levels of NO than the positive control LPS. However, the NO suppression effect by ACP-DW was so intense that lower amount (80-100 μg/mL) suppressed NO production to the level of the control. The effect was attributed to the expression of the iNOS. Then, Raw 264.7 cells were stimulated with the LPS and expression of COX-2 protein level was analyzed depending on the Aloe suspension culture product treatment. The results showed that the ACP-DW supplemented medium did not express COX-2 by itself, and LPS stimulated COX-2 expression was slightly decreased. On the other hand, realtime-PCR analysis of the expression of inflammatory cytokine showed that IL-1β and TNF-α expression was highly suppressed in the ACP- distilled water supplemented medium.

미생물,발효,생물공학 : 새송이버섯 biomass를 위한 최적배양 조건

김명욱 ( Myung Uk Kim ),권오준 ( Oh Jun Kwon ),우희섭 ( Hi Seob Woo ),조영제 ( Young Je Cho ) 한국응용생명화학회 2007 Applied Biological Chemistry (Appl Biol Chem) Vol.50 No.1

활오징어(Todarodes pacificus)의 개별 포장 유통시스템 확립

김명욱(Myung Uk Kim),조영제(Young Je Cho) 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.7

A packaging and distribution system for transferring individual live squids at low temperature was developed and compared to a conventional bulk container system. Ten live squids in individual packages were stored in a large container at low temperature (0~10°C). Live squids in individual packages at 6°C showed a survival rate of 84% up to 72 hours, after which the survival rate decreased. However, the survival rate remained at 60% up to 120 hours. Further, the squids survived up to a maximum of 7 days. Optimum temperature was 5°C, and the survival rate of the packages was 70% when stored at 5°C for 96 hours. A distribution test was carried out using a refrigerator truck at 5°C, and the results showed a 100% survival rate up to 16 hours and over 90% survival rate after 20 hours. A rectangular container was the most favorable when loading the container into the refrigerator truck. In testing the required volume of supplied seawater, 100% survival rate was observed over 15 hours with 20 L of sea water or more. Therefore, a single squid needed 2 L of seawater. After refrigerator truck transportation, optimum temperature for fish tank storage was 5°C, at which the survival rate was over 90% up to 72 hours. Using a refrigerator truck at 5°C, live squids survived up to 7 days, maintaining marketability.

미생물,발효,생물공학 : 송이버섯 biomass를 위한 균사체 배양 조건

김명욱 ( Myung Uk Kim ),조영제 ( Young Je Cho ) 한국응용생명화학회 2006 Applied Biological Chemistry (Appl Biol Chem) Vol.49 No.4

단일 림프절 전이 위암의 분석

김홍(Hong Kim),홍우성(Woo Sung Hong),정인호(In Ho Jeong),윤태일(Tae Il Yoon),김봉완(Bong Wan Kim),김영배(Young Bae Kim),조용관(Yong Kwan Cho),김명욱(Myung Wook Kim),한상욱(Sang Uk Han) 대한외과학회 2005 Annals of Surgical Treatment and Research(ASRT) Vol.68 No.4

선천성 수신증을 위한 Onen 등급 분류법의 임상적인 타당성

우다은,임명희,김명욱,김세윤,박용훈,Woo, Da Eun,Lim, Myung Hee,Kim, Myung Uk,Kim, Sae Yoon,Park, Yong Hoon 대한소아신장학회 2014 Childhood kidney diseases Vol.18 No.2

목적: 선천성 수신증은 신장 초음파 검사를 통해 조기 진단이 가능하며 초음파 소견과 질환의 예후가 연관성을 가진다. 기존 수신증의 분류법이 예후나 치료 방법 결정의 기준이 될 수 있는지에 대한 논란이 있어 Onen 분류법이 새로 제시되었다. 이에 선천성 수신증을 APDRP, SFU 및 Onen 분류법으로 비교하였을 때 Onen 분류법이 단일 수신증뿐만 아니라 복합 수신증에서도 타당한지 알아보고자 하였다. 방법: 2002년 1월부터 2011년 12월까지 선천성 수신증으로 진단받은 203명의 환자 중 추적 관찰이 가능했던 153명(204신단위)의 의무 기록을 후향적으로 검토하였다. 이들의 신장 초음파 영상을 분석하여 APDRP를 측정하고 SFU 및 Onen 분류법으로 수신증의 등급을 나누었다. 단일 또는 복합 수신증인지 조사하고, 만 2세경의 수신증의 임상 경과를 조사하여 자연 소실, 신기능 저하, 요로 폐쇄, 요로 감염 및 수술적 치료 여부를 조사하였다. 결과: 선천성 수신증에서 SFU-1등급과 Onen-1등급의 경우 단일 수신증이, SFU-4등급과 Onen-3등급의 경우는 복합 수신증이 많았다. 단일 수신증 군에서 만 2세까지 경과를 보면, 세 분류법 모두 수신증이 경미한 경우 자연 소실 빈도가 높았고 복합 수신증에서도 같은 결과를 보였다. 신기능 저하의 빈도는 단일 수신증은 Onen 분류법에 의한 등급 사이에 유의한 차이가 없었으나, 복합 수신증에서는 유의한 차이가 있었다. 요로 폐쇄와 수술적 치료의 빈도는 단일 수신증일 때 세 분류법 모두 고등급 수신증에서 유의하게 높았다. Onen 분류법의 고등급 수신증은 다른 분류법에 의한 것들과 비교하여 임상적 경과에 유의한 차이가 없었다. 결론: Onen 분류법은 APDRP 또는 SFU 분류법과 마찬가지로 단일 선천성 수신증의 임상 경과를 반영하며 복합 수신증에서 다른 분류법과 달리 신기능의 예후와 연관성이 있었으나, 고등급 수신증에서는 각 분류법 사이에 유의한 차이가 없어서 Onen 분류법의 장점을 확인할 수 없었다. Purpose: The purpose of this study was to compare the validity of Onen's alternative grading system (AGS) with that of the APDRP and SFU grading systems in patients with isolated and complicated congenital hydronephrosis. Methods: We retrospectively reviewed the medical records of 153 patients (204 renal units) diagnosed with congenital hydronephrosis between January 2002 and December 2011. We classified patients into 2 groups; isolated or complicated hydronephrosis. All renal units were graded according to anterior-posterior diameter of renal pelvis (APDRP), Society for Fetus Urology (SFU) and Onen's grading systems. We analyzed the prognosis of hydronephrosis, according to each grading system, at 2 years of age. Results: There were 152 renal units with isolated hydronephrosis and 52 renal units with complicated hydronephrosis. The isolated hydronephrosis group had a lower grade according to Onen's AGS, and showed more frequent spontaneous remission by 2 years of age. There was more frequent obstruction (P =0.000) and surgical treatment (P =0.000) of units with high-grade hydronephrosis according to Onen's AGS. In the complicated hydronephrosis group the frequencies of spontaneous remission (P =0.015) and renal dysfunction (P =0.013) were significantly higher than those in high-grade hydronephrosis, as indicated by Onen's AGS. There were no significant differences in clinical outcomes among the highest grade groups, according to the 3 systems, in either isolated or complicated hydronephrosis. Conclusion: Onen's AGS reflects the prognosis of hydronephrosis as well as other grading systems in those with isolated hydronephrosis. It was better predictor of renal dysfunction in those with complicated hydronephrosis. However, Onen's AGS was not superior to the other grading systems in terms of predicting prognosis, especially in high-grade hydronephrosis.

앨리스 죽이기

김상규,성상민 한국독립영화협회 2019 한국독립영화협회 기타간행물 Vol.2018 No.-

위암의 진행과 간세포성장인자(HGF), Met 그리고 E-cadherin 발현간의 상호관계

한상욱(Sang-Uk Han),주희재(Hee-Jae Joo),이재호(Jae-Ho Lee),김욱환(Wook-Hwan Kim),조용관(Yong-Kwan Cho),김명욱(Myung-Wook Kim) 대한외과학회 1998 Annals of Surgical Treatment and Research Vol.55 No.1