자궁경부암에서 혈관 내피 성장인자 ( VEGF ) 및 VEGF Mrna 의 발현에 대한 연구

이선경(Seon Kyung Lee),김승보(Seung Bo Kim),지성길(Sung Gil Chi),염윤석(Yoon Seok Yum),이주희(Ju Hee Lee) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.1

N/A Objective : Angiogenesis, the formation of blood vessels by sprouting from pre-existing ones, is essential for the growth of solid tumors beyond 2~3mm in diameter and for tumor metastasis. Vascular endothelial growth factor (VEGF), is known as vascular permeability factor(VPF) and mediates vascularization and tumor-induced angiogenesis. This study examined the potential of growth, invasion, and metastasis of uterine cervical carcinomas associated with neovascularization. Methods : From January 1996 to December 1999, at the Department of Obstetrics and Gynecology, Kyung-Hee University Hospital, 37 uterine cervical carcinomas and 7 normal cervical tissues were obtained and the samples were immediately frozen and stored at -70℃. Immunohistochemical staining for VEGF was carried out to study VEGF localization, and the levels of VEGF subtype mRNAs were determined by quantitative RT-PCR in specimens. The relation between VEGF subtypes expression of cervical cancers was analysed. Results : The positive staining for VEGF is seen dominantly in the cytoplasm of the cancer cells, and faintly in interstitial cells. The intensity of staining was stronger in squamous carcinomas than in adenocrcinomas, but there was no significant difference (p>0.05). Quantitative RT-PCR analysis demonstrated significantly increased VEGF121/VEGF165 mRNA expression levels (>0.56/>0.72) in 21 (56.8%) and 15 (40.5%) of 37 cervical carcinomas comparing to control groups (mean: 0.28/0.36). There was no obvious relationship between VEGF121/VEGF165 mRNA expression levels and the clinical parameters examined including age, pathology, differentiation, tumor size, lymphovascular space invasion, LN involvement and invasion depth except clinical stage (p<0.05). Conclusions : The overexpression of VEGF mRNA may be an important contributing factor in cervical carcinomas. There is no significant differenece of VEGF mRNAs levels according to clinical parameters, so it seems that the expression of VEGF is involved in the promotion of angiogenesis on cervical cancer and plays an important role in early invasion.

Immunoblot 법을 이용한 간흡충항원(肝吸蟲抗原)의 발육단계별(發育段階別) 항원성분석(抗原性分析)에 관한 연구(硏究)

이선경,주경환,정명숙,임한종,Lee, Seon-Kyung,Joo, Kyoung-Hwan,Chung, Myung-Sook,Rim, Han-Jong 한국농촌의학지역보건학회 1991 농촌의학·지역보건 Vol.16 No.1

Serodiagnosis of Clonorchis sinensis infections will probably be a first choice tool for screening of clonorchiasis in a future because of increasing difficulties in collection and examination of stools. The sensitive test such as ELISA can he used effectively. However there are some limitations in serological diagnosis for the detection of serum antibody. One of the major problems is the non-specificity of the antigens which produce cross reaction with other helminthic infection sera. To solve this problem. many investigators have tried to purify the antigens used. In this study, we determined the antigenic profile of the crude saline extract antigen of C. sinensis at early developmental stage based on SDS-PAGE and immunoblotting techniques for the purpose of understanding the nature of C. sinensis worm antigen The following results were obtained : 1) The SDS-PAGE showed many protein hands ranging from 10Kd to 91Kd relative molecular weight. Among them, 66, 46, 40, 33, 27, 24, 16, 14 and 10Kd bands were observed as a principle bands. The protein components of C. sinensis changed chronologically during their early developmental period. 44Kd band was stained unclearly in antigen of 2 weeks worm, but changed to concentrated state in antigen of 5 weeks worm. 35Kd band was found in antigen of 2 weeks worm, however this band was disappeared in antigen of 5 weeks worm. 22Kd band also lost its staining property gradually. 2) In spite of differences in antigenic profile, there was no differences in the data obtained by microplate ELISA using each antigen preparation. Absorbance value began to rise in between 2 to 3 weeks after infection. 3) By EITB. serum antibody recognized major protein bands with molecular weight of 91, 85, 63, 46, 40, 33, 24, 14 and 10Kd hand respectively. Among them 66, 33, 17 and 14Kd bands were observed as non-specific band because they reacted even in normal control sera. Generally, gradual increase of positive reactions were observed as the infection period of C. sinensis was prolonged. In other hand, the reaction of 10Kd hand did not occurred when 26th week sera was tested. 4) The positive reactions using antigens of 2 weeks worm, especially on 40 and 24Kd bands, were most strong and sharply demarcated compared to those of 3~5 weeks worm antigen.

부인암에서의 Telomerase 활성도와 자궁경부암 조직에서의 HPV E6 의 발현과 Telomerase 활성도의 상관관계

유용신(Yong Sin You),이선경(Seon Kyung Lee),김승보(Seung Bo Kim),지성길(Sung Gil Chi) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.8

N/A Object : In this study, to evaluate the putative role of telomerase in gynecologic malignancies (cervical ca, ovarian ca, endometrial ca), we measured telomerase activity in malignant gynecologic tumor tissues and normal tussues, and compared it with prognostic factors in cervical cancer. To evaluate the correlation of telomerase activity and human papillomavirus (HPV) infection in cervical cancer, the analysis of HPV E6 gene was performed. Method : Specimens were obtained from 51 women who underwent gynecologic radical operation and 13 normal tissues (from December 1995 to December 1996) in the Department of Obstetrics and Gynecology, Kyung-Hee Univ. Medical Center. With Telomerase PCR ELISA (Boehring Mannheim), modified TRAP (Telomere Repeat Amplication Protocol), we examined telomerase activity of 32 cervical carcinomas, 11 ovarian carcinomas, 8 endometrial carcinomas, 5 normal cervical tissues, 4 normal ovarian tissues and 4 normal endometrial tissues. The analysis of HPV E6 gene was performed by PCR amplication. We compared the abnormally high telomerase activity with prognostic factors, also compared the telomerase activity with the expression of HPV E6 gene in cervical cancer tissues. Result : We detected the abnormally high telomerase activity in all cervical carcinomas, 10 of 11 (90.9%) ovarian carcinomas, 6 of 8 (75.0%) endometrial carcinomas, but couldn't detect in each normal tissues. There was statistically no significant difference of telomerase activity levels according to age, clinical stage, pathology, differentiation, LN involvement, depth of invasion and tumor size except lymphovascular space invasion in cervical carcinomas (p<0.05). According to the analysis of HPV E6 gene, 29 of 32 (90.6%) in 32 cervical cancer tissues showed HPV E6 positivity. So it was considered that telomerase activation was closely related with the expression of HPV E6 gene. Conclusion : Telomerase activation is associated with immortalization or malignant transformation of gynecologic cancers. The expression of HPV E6 gene is considered to activate telomerase in cervical cancer.

자궁경부암 조직에서의 Telomerase 활성도와 hTERT mRNA 발현 HPV E6 검출의 상관관계에 관한 연구

홍종우(Jong Woo Hong),이선경(Seon Kyung Lee),반태학(Tae Hak Ban),지성길(Sung Gil Ji) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.3

N/A Objective: Telomerase is a ribonucleoprotein enzyme which stabilizes chromosomal structure, thereby inducing cellular immortality. We investigated telomerase activity and human telomerase reverse transcriptase (hTERT) mRNA expression in relation to high-risk human papillomavirus (HPV) DNA presence in cervical carcinomas. Met hods: From December 1995 to December 1999, at the department of obstetrics and Gynecology of Kyung-Hee University Hospital,32 cervical carcinomas and 5 corresponding nontumor cervical tissues were obtained and the samples were immediately frozen and stored at -70℃. Telomerase activity was measured by using telomerase PCR ELISA, a modified version of the TRAP. Analysis of the expression of hTERT mRNA was performed by quantitative RT-PCR and the analysis of the HPV E6 gene was performed by DNA-PCR. Result: All of the carcinomas examined exhibited strongly positive for telomerase activity (OD>0.24), whereas telomerase activity was week or not found in the 5 corresponding nontumor cervical tissues. Quantitative RT-PCR analysis demonstrated significantly increased hTERT mRNA expression levels (>0.024) in most carcinomas comparing to control groups. There was no obvious relationship between telomerase activity levels and the clinical parameters examined including age, clinical stage, pathology, differentiation, tumour size, LN involvement and invasion depth except lymphovascular space invasion (p=0.03). In the correlation between the levels of hTERT mRNA expression and telomerase activity, correlation index which was 0.916,shows high correlation (p=0.01). According to the analysis of HPV E6 gene,29 of 32 (90.6%)carcinomas showed HPV E6 positivity. Conclusion: There is a strong association between telomerase activity and hTERT mRNA expression, and up-regulation of hTERT probably plays a role in the progression of cervical carcinomas. Telomerase is at least partially activated by viral oncogenes of high-risk types. There is no obvious relationship between telomerase activity levels and the clinical parameters except LSVI (p=0.03). These findings provides that telomerase may play an important role in the early stage of carcinogenesis.

선행 제왕절개술 후 질식 분만: 성공의 예측인자들

동서연 ( Seo Yun Tong ),김여홍 ( Yeo Hong Kim ),최영준 ( Young Jun Choi ),김소라 ( So Ra Kim ),이보연 ( Bo Yon Lee ),이선경 ( Seon Kyung Lee ),허주엽 ( Chu Yeop Huh ),김승보 ( Seong Bo Kim ) 대한주산의학회 2003 Perinatology Vol.14 No.4

목적: 선행제왕절개 후 질식분만을 시도한 산모들 중 성공군과 실패군을 비교하여 성공을 예측할 수 있는 인자를 알아보고자 하였다. 방법: 1997년 3월부터 2003년 2월까지 경희대학교 의과대학 산부인과에서 선행제왕절개 후 질식분만을 위하여 내원한 환자중 질식분만의 금기가 아닌 경우 위험요소 등에 대하여 충분히 상의한 후 분만을 시도한 382명의 환자를 대상으로 하였다. 대상군의 의무기록을 통하여 분만당시 산모의 나이, 분만력, 제태연령, 신생아 몸무게, 입원시 자궁경부 상태 및 Bishop score, 분만의 진행과정, 결과 및 합병증을 조사하여 성공군과 실패군의 차이를 비교분석 하였다. 결과: 292(76.5%)명이 질식분만에 성공하였다. 여러 인자 중 태아의 몸무게(odds ratio, 0.2 ; 95% confidence interval (CI), 0.1-0.4), 입원시 Bishop score(odds ratio, 1.8 ; 95% CI, 1.5-2.1), 질식분만의 과거력(odds ratio, 3.6 ; 95% CI, 1.1-11.6)이 VBAC의 성공과 관련이 있었다. 또한 선행제왕절개시 적응증이 태위이상인 경우에 성공률이 높은 것으로 나타났다(odds ratio, 3.2 ; 95% CI, 1.2~8.6). 입원시 Bishop score로 VBAC의 성공을 예측할 수 있는 지 알아보기 위하여 receiver-operating characteristic curve 분석을 시행하였고, 5점을 cut-off로 하였을 때 47.4%의 민감도와 87.6%의 특이도를 보였다(AUC, 0.765 ; p<0.05). 결론: 출생체중, 질식분만의 과거력, 입원시 Bishop score가 선행제왕절개 후 질식분만을 고려하는 경우 성공의 예측인자로서 유용하게 사용되어 질 수 있을 것이다. Objective: The purpose of this report is to determine the predictable factors for successful vaginal birth after cesarean (VBAC). Methods: In this study, 382 women who attempted VBAC at Kyung-Hee university hospital were included. The medical records of them were reviewed retrospectively. Informations was collected about demographics, medical history, obstetric history, neonatal birth weight, complications, treatment, and outcome of the index pregnancy. Results: The overall success rate was 76.5% (292 cases). Among variables, neonatal birth weight (odds ratio, 0.2; 95% confidence interval, 0.1-0.4), Bishop score at admission (odds ratio, 1.8; 95% confidence interval, 1.5-2.1 ; p<0.05), and history of vaginal delivery (odds ratio, 3.6 ; 95% confidence interval, 1.1-11.6 ; p<0.05) were significant. The Bishop score showed most significant relationship with successful VBAC. The Bishop score 5 at admission had a sensitivity of 47.4% and a specificity of 87.6% in the prediction of successful VBAC. Malpresentation as indications of previous cesarean delivery showed more tendency to succeed than others. Conclusion: Several factors such as birth weight, Bishop score at admission, and history of vaginal delivery may be used to predict the success of VBAC.

아동의 문제행동에 영향을 미치는 위기요인과 보호요인의 이론적 과정모델

이선경(Seon Kyung Lee),이재연(Jae Yeon Lee) 한국아동학회 1998 아동학회지 Vol.19 No.2

The theoretical process model was used to study problem behavior of children growing up at-risk. A sample of 339 children living in at-risk families were selected from among 2, 134 children in grades 4, 5, and 6 of five elementary schools in Seoul. The instruments were five risk factor scales and four protective factor scales. The Child Behavior Checklist was also used. The results of the hypothetical model of children at-risk showed that the factor of socioeconomic status led to positive child rearing attitudes in mothers, which is turn influenced children`s problem behavior indirectly. In contrast, stressful life events and mother`s depression influenced children`s problem behavior directly. However, negative family functioning, marital discord and father`s depression did not influence mother`s child rearing attitudes or children`s problem behavior. Mother`s child rearing attitudes directly influenced children`s social competence and academic achievement and indirectly influenced their problem behavior. Academic achievement did not influence problem behavior directly, but social competence influenced it directly. Mother`s child rearing attitude was the most significant factor affecting children`s adaptation.

3-5세 누리과정 교사용 지도서에 수록된 동화?동시 분석

이선경 ( Seon Kyung Lee ),김경의 ( Kyung Eui Kim ) 한국어린이문학교육학회 2013 어린이문학교육연구 Vol.14 No.3

본 연구의 목적은 3-5세 누리과정 교사용 지도서에 수록된 동화와 동시를 분석함으로써 그 경향성을 이해하고, 이를 통해 현장 교사들에게 동화, 동시 지도의 방향 설정을 위한 기초 자료를 제공하는데 있다. 3-5세 누리과정 교사용 지도서에 수록된 동화 274편은 장르별, 구조별, 생활주제별, 전달 매체별로 구분하여 분석하였고, 동시 85편은 장르별, 유형별, 생활주제별, 전달매체별로 구분하여 분석하였다. 분석결과는 다음과 같다. 첫째 동화의 경우 장르별로는 환상동화, 구조별로는 문제해결, 생활주제별로는 나와 가족, 전달매체별로는 플래쉬 동화가 가장 높은 비율로 나타났다. 둘째 동시의 경우 장르별로 형식적 분류에서는 자유 동시가 가장 많은 분포를 보였고, 내용상 분류에서는 모두 서정 동시로 나타났으며, 유형별로는 열거와 순서, 생활주제별로는 생활도구, 전달매체별로는 플래쉬가 높은 비율로 나타났다. 이러한 결과는 현장에서 지도서를 활용하여 동화, 동시 수업을 계획하고 실행할 때 균형을 고려할 필요가 있음을 제시하였다. The purpose of this study is to understand the trend in the fairy tales and children`s poetry used in the Teachers` Guidebooks for 3 to 5-year-olds` NURI Curriculum by analysis thereof, and to provide the basic materials helpful for teachers to set the teaching direction of fairy tales and children`s poetry. The subjects of this study are 274 fairy tales and 85 pieces of children`s poetry listed in the Teacher`s Guidebooks for 3 to 5-year-olds` NURI Curriculum. The statistical data of the fairy tales was analyzed by categories such as genre, story structure, theme and media, whereas that of the children`s poetry was analyzed by categories such as genre, type, theme and media. The results of this study are as follows: In the case of the fairy tales, the highest percentage is taken by i) fantastic fiction in terms of genre, ii) problem solution in terms of story structure, iii) ``me and family`` in terms of theme, and iv) flash in terms of media, respectively. With regard to the genre of children`s poetry, free-form children`s poetry takes the largest proportion in terms of formal classification, whereas all of the children`s poetry is lyrical in terms of contents classification. Also, in terms of the type, the theme and the media of children`s poetry, the largest percentage is taken by lists and sequence, life tools and flash, respectively. These conclusions suggest that, when planning and implementing the lessons of fairy tales and children`s poetry in the field by using the Teachers` Guidebooks, it is necessary to consider the balance.

아로마테라피의 연구 동향과 한의학적 활용에 대한 고찰

이선경(Seon Kyung Lee),윤미영(Mi Young Yoon),손경우(Kyung Woo Sohn),채한(Han Chae) 한의병리학회 2011 동의생리병리학회지 Vol.25 No.3

보육교사들의 누리과정 실행경험과 지원요구

이선경(Lee, Seon Kyung),고경미(Koh, Kyung Mee) 한국유아교육·보육복지학회 2013 유아교육·보육복지연구 Vol.17 No.4

본 연구는 보육교사를 대상으로 심층면담을 통해 3-5세 연령별 누리과정에 대한 실행경험을 탐색하고 지원요구를 고찰하는데 목적이 있다. 연구결과 보육교사들은 누리과정의 시행이 유보통합에 기여했지만, 관련 교사교육은 크게 도움이 되지 않았고, 누리과정 교사용 지도서와 상업적 교수자료에 의지한 실행이 이루어졌고 그 과정에서도 갈등과 혼란이 있는 것으로 나타났다. 보육교사들은 부모들의 특별활동에 대한 요구와 누리과정의 현실적 실행 사이에서 어려움을 겪었고, 표준보육과정과 누리과정을 모두 숙지해야 하는 이중부담감을 느끼고 있었다. 보육교사들은 자긍심을 고취하는 심리적 지원, 전문성 향상을 위한 교육적 지원, 정부차원의 홍보를 위한 사회적 지원, 평가인증지표 정립을 위한 도구적 지원을 요구하였다. The purpose of this study was to investigate childcare teachers' practice experiences of the Nuri curriculum for 3-5 year old children, and to identify the supports they desire. Although teachers agreed that the Nuri program provided a unified standard curriculum for both kindergartens and childcare centers, they felt that teacher training was not effective, and found conflicts and confusions during the education practice based on official Nuri and commercial teaching materials. They suffered difficulties from the discrepancies between practical exercise of the Nuri curriculum and parents's demands on extra-curricular activities. In addition, it was a double burden for teachers to master the Nuri curriculum as well as the existing standard curriculum. The supports requested by teachers include psychological support for teachers' self-esteem, educational support enhancing their expertise, social support for advertising the Nuri curriculum, and instrumental support for the evaluation and certification of the Nuri practice.

어머니의 애정적, 거부적 양육태도 및 유아의 정서지능과 사회적 능력 간의 구조모형 분석

이선경(Lee, Seon Kyung),최영숙(Choi, Yeoung Sook),강상(Kang, Sang) 한국유아교육·보육행정학회 2014 유아교육·보육복지연구 Vol.18 No.1

본 연구는 유아의 사회적 능력에 영향을 미치는 변인에 대한 이론적 근거를 바탕으로 어머니의 애정적, 거부적 양육태도, 유아 정서지능, 사회적 능력 간의 구조적 관계를 밝히고, 어머니의 애정적, 거부적 양육태도가 유아의 사회적 능력에 영향을 미치는 과정에서 정서지능의 매개효과는 어떠한지 살펴보았다. 전라북도 유아교육기관에 재원중인 만 5세 유아와 그들의 어머니 257쌍을 대상으로 어머니 애정적, 거부적 양육태도, 유아 정서지능, 사회적 능력을 조사하여 구조모형 분석을 하였다. 연구결과, 어머니의 애정적, 거부적 양육태도, 정서지능은 사회적 능력에 직적접인 영향을 미치며 또 애정적 양육태도는 정서지능을 매개로 하여 간접적인 영향도 미치는 것으로 나타났다. 이러한 결과를 토대로 유아의 사회적 능력 발달을 위한 부모-자녀 관계에 대한 시사점을 제시하고 후속연구를 위한 제언을 하였다. This study clarified the structural relationship between mothers' affectionate-rejective child-rearing attitudes and children's emotional intelligence and social competence on the basis of the theoretical grounds regarding the variables affecting children's social competence, and researched into how the mediation effect of emotional intelligence operated while the mothers' affectionate-rejective child-rearing attitudes affected the children's social competence. A structural model analysis has been done by examining the mothers' affectionate-rejective child-rearing attitudes and children's emotional intelligence and social competence, with the subjects being 257 pairs of children under 5 attending early childhood educational institutions in Cheollabuk province and their mothers. The results of this study are as follows: 1) Mothers' affectionate-rejective child-rearing attitudes and children's emotional intelligence have direct influence on the social competence. 2) Affectionate child-rearing attitudes have also indirect influence, while emotional intelligence serving as the mediation factor. Based on these results, this study presented suggestions about the parents-children relationship for improving the children's social competence, and suggested some proposals for following studies.