Superoxide에 의존하여 내피세포에서 유리되는 이완성 물질의 특성에 대한 실험적 연구

이기남,이원석,임병용,홍기환,Lee, Gi-Nam,Lee, Won-Suk,Rhim, Byung-Yong,Hong, Ki-Whan The Korean Society of Pharmacology 1990 대한약리학잡지 Vol.26 No.2

최근 본 교실에서는 two-bath system을 이용하여 혈관 내피세포에서 superoxide에 의존한 혈관 이완성 물질을 동정하여 발표하였다. 본 실험에서는 상기 system을 이용하여 돼지의 관상동맥 내피세포에서 유리되는 superoxide에 의존한 이완성 물질이 고양이의 흥부 대동맥 내피 및 소의 대동맥 배양내피세포에서 얻어진 이완성 물질에 의한 이완과 매우 유사함을 관찰하여 다음과 같은 결과를 얻었다. 1. 고양이 흥부 대동맥, 돼지 관상동맥의 내피세포 및 소 대동맥 배양 내피세포 등에서 유리되는 superoxide에 의존한 이완 물질은 모두 유사한 이완 작용을 나타내었다. 2. 돼지 관상 동맥 내피세포에서 유리되는 superoxide 의존성 이완 물질이 고양이의 흥부 대동맥 내피세포나 소의 대동맥 배양 내피세포에서 유리되는 이완 물질과는 다소 다른 점도 있었다. 즉, 돼지 관상동맥 내피세포에서 유리되는 이완 물질의 작용은 catalase나 superoxide dismutase(SOD)에 의하여 억제되었으나, 후자의 두 동맥 내피세포에서 유리되는 이완 물질은 SOD에 의해서만 억제되었다. 3. 이러한 이완성 물질들의 생성은 여러 lipoxygenase억제제인 gossypol, nordihydroguaiaretic acid, AA 861 및 eicosatetraynoic acid 등의 전처치에 의하여 봉쇄되었다. 4. Cyclooxygenase 억제제인 indomethacin이 나 cytochrome P-450 monooxygenase 억제제인 proadifen과 cimetidine에 의하여는 봉쇄되지 아니하였다. 이상의 결과로부터 이러한 이완성 물질들은 비록 각기 다른 종의 동물 모델에서 얻었다고 하더라도 장기에 따라 다소 반응의 차이는 있으나 동질성 이완 물질이며, 나아가 이러한 이완성 물질은 여러 조직의 허혈-재관류 손상에 있어서 병리생리학적으로 관련될 것으로 사료된다. We recently reported a development of an experimental system which can identify the release of a superoxide-dependent vasorelaxant factor from endothelial cells using a two-bath system. In the present work, we further exploited the above system and observed whether the superoxide-dependent relaxing factor(s), released from the porcine coronary artery (PCA) endothelium, was similar in relaxation to those obtained from cat thoracic aortic endothelium and cultured endothelial cells of bovine aorta. However, there was observed a novel difference among the former one and the latter two relaxing factors; the release of relaxing factor from PCA endothelium can be inhibited either by catalase or by superoxide dismutase (SOD), whereas the latter two can be inhibited only by SOD. It was further attempted to characterize the synthetic mechanisms of the relaxing factors: (1) They were readily inhibited by various lipoxygenase inhibitors (gossypol, nordihydroguaiaretic acid, AA 861, and eicosatetraynoic acid). (2) They were not inhibited by cyclooxygenase inhibitor (indomethacin) and by cytochrome P-450 monooxygenease inhibitors (proadifen and cimetidine). Thus, it is likely that these relaxing factors, although obtained from different species, show common functional roles of arteriolar relaxation. It is suggested that they are related to pathophysiological involvement of various tissue ischemia-reperfusion injuries.

Superoxide에 의존하여 내피세포에서 유리되는 이완성 물질의 특성에 대한 실험적 연구

이기남(Gi-Nam Lee),이원석(Won-Suk Lee),임병용(Byung-Yong Rhim),홍기환(Ki-Whan Hong) 대한약리학회 1990 대한약리학잡지 Vol.26 No.2

최근 본 교실에서는 two-bath system을 이용하여 혈관 내피세포에서 superoxide에 의존한 혈관 이완성 물질을 동정하여 발표하였다. 본 실험에서는 상기 system을 이용하여 돼지의 관상동맥 내피세포에서 유리되는 superoxide에 의존한 이완성 물질이 고양이의 흥부 대동맥 내피 및 소의 대동맥 배양내피세포에서 얻어진 이완성 물질에 의한 이완과 매우 유사함을 관찰하여 다음과 같은 결과를 얻었다. 1. 고양이 흥부 대동맥, 돼지 관상동맥의 내피세포 및 소 대동맥 배양 내피세포 등에서 유리되는 superoxide에 의존한 이완 물질은 모두 유사한 이완 작용을 나타내었다. 2. 돼지 관상 동맥 내피세포에서 유리되는 superoxide 의존성 이완 물질이 고양이의 흥부 대동맥 내피세포나 소의 대동맥 배양 내피세포에서 유리되는 이완 물질과는 다소 다른 점도 있었다. 즉, 돼지 관상동맥 내피세포에서 유리되는 이완 물질의 작용은 catalase나 superoxide dismutase(SOD)에 의하여 억제되었으나, 후자의 두 동맥 내피세포에서 유리되는 이완 물질은 SOD에 의해서만 억제되었다. 3. 이러한 이완성 물질들의 생성은 여러 lipoxygenase억제제인 gossypol, nordihydroguaiaretic acid, AA 861 및 eicosatetraynoic acid 등의 전처치에 의하여 봉쇄되었다. 4. Cyclooxygenase 억제제인 indomethacin이 나 cytochrome P-450 monooxygenase 억제제인 proadifen과 cimetidine에 의하여는 봉쇄되지 아니하였다. 이상의 결과로부터 이러한 이완성 물질들은 비록 각기 다른 종의 동물 모델에서 얻었다고 하더라도 장기에 따라 다소 반응의 차이는 있으나 동질성 이완 물질이며, 나아가 이러한 이완성 물질은 여러 조직의 허혈-재관류 손상에 있어서 병리생리학적으로 관련될 것으로 사료된다. We recently reported a development of an experimental system which can identify the release of a superoxide-dependent vasorelaxant factor from endothelial cells using a two-bath system. In the present work, we further exploited the above system and observed whether the superoxide-dependent relaxing factor(s), released from the porcine coronary artery (PCA) endothelium, was similar in relaxation to those obtained from cat thoracic aortic endothelium and cultured endothelial cells of bovine aorta. However, there was observed a novel difference among the former one and the latter two relaxing factors; the release of relaxing factor from PCA endothelium can be inhibited either by catalase or by superoxide dismutase (SOD), whereas the latter two can be inhibited only by SOD. It was further attempted to characterize the synthetic mechanisms of the relaxing factors: (1) They were readily inhibited by various lipoxygenase inhibitors (gossypol, nordihydroguaiaretic acid, AA 861, and eicosatetraynoic acid). (2) They were not inhibited by cyclooxygenase inhibitor (indomethacin) and by cytochrome P-450 monooxygenease inhibitors (proadifen and cimetidine). Thus, it is likely that these relaxing factors, although obtained from different species, show common functional roles of arteriolar relaxation. It is suggested that they are related to pathophysiological involvement of various tissue ischemia-reperfusion injuries.

동과가 streptozotocin에 의해 손상된 대뇌신경세포에 미치는 영향

이환봉(Whan Bong Lee),이강창(Kang Chang Lee),이기남(Ki Nam Lee),홍기연(Gi Youn Hong),석승한(Seung Han Suk),조정구(Chung Cu Cho),정선관(Seon Kwan Jung),허정무(Jung Mu Hur),이상복(Sang Bork Lee),서은아(Eun A Seo),송호준(Ho Jun Song),이영 한의병리학회 2002 동의생리병리학회지 Vol.16 No.3

경요도적 절제술 후 방광 이행세포암의 재발에 관계하는 요소들

남기동,구봉식,윤성국,박병호,남경진,최종철,이기남,이영일,정덕환,Nam, Gi-Dong,Koo, Bong-Sik,Yoon, Sung-Guk,Park, Byeong-Ho,Nam, Gyeong-Jin,Choi, Jong-Cheol,Lee, Gi-Nam,Lee, Yeong-Il,Jeong, Deok-Hwan 대한영상의학회 1998 대한영상의학회지 Vol.38 No.4

Purpose: To evaluate factors related to the recurrence of TCC(transitional cell carcinoma) in the urinary bladder after transurethral resection of bladder tumor(TUR-BT). Materials and Methods : We retrospectively reviewed 54 patients in whom TCC (transitional cell carcinoma) after TUR-BT had been confirmed. Recurrence was evaluated by US, CT, cystoscopy and urine smear during the follow-up period of 6 months. The multiplicity, shape, size, and calcification of TCC, as revealed by radiologic studies, were evaluated retrospectively before TUR-BT. After TUR-BT, the histologic grade and pathologic stage of TCC were evaluated. Results : According to the multiplicity of TCC, the recurrence rate was 66.7% in the multiple type and 28.6% in the single type(p=0.039);according to shape, this rate was 61.5% in the sessile type and 29.3% in the pedunculated type(p=0.0505), and according to mass size, the rate was 41.7% in tumors more than 3cm in diameter and 35.7% in tumors less than 3cm(p=0.706). In the presence of calcification, the recurrence rate was 40.0% and in its absence, this rate was 36.7%(p=0.885). Pathologically, the higher the grade and stage of TCC, the higher the recurrence rate(respectively p=0.010 and 0.041). Conclusions : Radiologically, multiple and/or sessile type TCC had a higher recurrence rate than the single and/or pedunculated type. Pathologically, when the grade and stage of bladder tumor were higher, recurrence rates were higher.

當歸飮子 水抽出液이 抗ALLERGY 反應과 MOUSE의 免疫細胞機能에 미치는 影響

노석선,이기남,No, Seok-Seon,Lee, Gi-Nam 대한한방안이비인후피부과학회 1991 한방안이비인후피부과학회지 Vol.4 No.1

This study were done to know the effects of Dangkiyeumja on the in vivo and in vitro immune responses of mice. The recipes of Dangkiyeumja used in this study enhanced such, cellular functions of immunocytes as phagocytic capacity of macrophages, rossett-eforming abilities of splenocytes and metabolic activities of lymphocytes, However, the same recipes decreased the formation of such reactive oxygen intermediates(ROI) as superoxide and hydrogenperoxide from the macrophages. The effects of the same recipes on the in vim immune responses was suppressive on the cellular immune response(CIR)measured by delayed-type hypersensitivity against dinitrofluorobenzene and mildly enhancing for the humoral immune response measured by antibody production against sheep red blood cells. The results of this study could be summarized as follow: 1. Administration of Dangkiyeumja enhanced the phagocytic activity of the murine macrophage. 2. Administration of Dangkiyeumja decreased the formation of ROI in the murine macrophage 3. Administration of Dangkiyeumja increased the number of the splenic rotte forming cells in the mouse. 4. Administration of DangKiyeumja did not effect the antibody production against sheep red blood cells. 5. Administration of Dangkiyeumja depressed the delayed-type hypersenitivity against dinitrofluoro benzene in the mouse. The result of this study suggest that Dangkiyeumja could ameliorate the hypersensitivity reactions by reducing the formation of ROI and decreasing the CIR without affecting the other functions of immunocytes.

국민학교(國民學校) 교과서(敎科書)의 보건의료(保健醫療) 내용분석(內容分析) 연구(硏究) - 한의학(韓醫學)을 중심(中心)으로 -

이선동,이기남,Lee, Seon-Dong,Lee, Gi-Nam 제3의학회 1996 제3의학 Vol.1 No.1

This study was subjectived elementary school books published between 1989 and 1991. There focused on the oriental medicine of all Health-Medical contents. The results of analysis for elementary school books were much less oriental medicine contents than the others.Also,it was negative trend in many contents like incorrect of historical incidence etc.That contents was not two medicine's complementarity but two division. This result did possible contribute to misunderstand and negative action toward oriental medicine in the elder age attitude. Oriental Medicine has been successive to the present and shall be continue to the future as natural korea culture. In order to continuity of normal Health-Medical culture and obtain of worldwide oriental medicine trend, it need to revise contents of elementary school books rapidly.

수두대상포진 폐렴의 고행상 CT 소견

김건일,이기남,Kim, Geon-Il,Lee, Gi-Nam 대한영상의학회 2003 대한영상의학회지 Vol.49 No.6

목적:수두대상포진 폐렴의 고해상 CT(HRCT)소견을 알아보고자 하였다. 대상과 방법:수두대상포진 폐렴으로 임상적 및 혈청학적으로 진단된 환자 중 HRCT를 얻은 성인 7명을 대상으로 하였다.1명은 신장이식을 받은 환자였고,나머지 6명은 정상 면역력을 가지고 있었다.HRCT에서 결절,폐경화,간유리 음영,흉막삼출 등의 유무를 관찰하였고,결절은 그 수와 크기,그리고 분포양상을 2명의 진단방사선과 의사가 후향적으로 조사하였다. 결과:수두대상포진 폐렴의 HRCT에서 결절 (n=7),폐경화 (n=3),미만성 간유리음영(n=1),흉 막삼출 (n=1)이 관찰되었다.결절은 2-10 mm 크기의 다발성 (n=6)이였고,전 폐야에 고르게 분포하는 경향을 보였다.미만성 간유리 음영과 소량의 양측성 흉막삼출을 보인 1예는 급성호흡곤란증후군이 수두대상포진 폐렴에 병발한 경우였다. 결론:수두를 앓고 있는 성인에서 2-10 mm 크기의 다발성 결절,주위에 간유리 음영을 동반한 결절,결절의 융합,폐경화 등이 HRCT에서 보일 때 수두대상포진 폐렴을 감별진단에 포함시켜야 할 것이다. Purpose: To describe the high-resolution CT findings of varicella-zoster pneumonia. Materials and Methods: Seven adult patients with clinically and serologically diagnosed varicella-zoster pneumonia underwent HRCT scanning. One had undergone a kidney transplant, and the others were immunocompetent. The HRCT findings were retrospectively analyzed by two radiologists in terms of the presence of a nodule, pneumonic consolidation, ground-glass attenuation and pleural effusion, and with regard to the number, size and distribution of nodules. Results: HRCT findings of varicella pneumonia included nodule (n=7), consolidation (n=3), diffuse groundglass attenuation (n=1) and pleural effusion (n=1). Nodules were mostly 2-10 mm in size, and multiple (n=6), and zonal predominancy was not apparent. One case in which HRCT demonstrated diffuse groundglass opacity and slight bilateral pleural effusion involved a patient in whom complications of acute respiratory distress syndrome occured. Conclusion: When HRCT demonstrates the presence of nodules 2-10 mm in size multiple nodules, or nodule surrounded by ground-glass attenuation, or the coalescence of nodules and consolidation is observed in adults with chickenpox, the varicella-zoster pneumonia should be included in the differential diagnosis

포공영(蒲公英) 전탕액(煎湯液)을 이용한 카드뮴 독성 해독(解毒) 효과연구

박재수,이종섭,이기남,Park Jae-Soo,Lee Jong-Sub,Lee Gi-Nam 대한예방한의학회 1997 대한예방한의학회지 Vol.1 No.1

A study on the antitoxic effects of Herba Taraxaci extracts against Cadmium Chloride Toxicity in Rats. This study was performed to find out the effect of taraxacum platy carpum against Cadmium toxicity. The experimental rats were divided into 5 groups such as control group, Cadmium alone treatment group, three simultaneous treatment groups of taraxacum platy carpum and cadmium. Rat were given pellets administration with three dosage of taraxacum platy carpum such as 4 mg/kg body weight for four weeks. The results were summarized as follows on: 1. The simultaneously administration of taraxacum platy carpum and cadmium significantly more decreased cadmium concentration in liver and kidney tissues compared to the administration of cadmium alone. (p<0.05) 2. The simultaneously administration of taraxacum platy carpum and cadmium significantly more increased metallothionein concentration in liver and kidney tissues compared to administration alone. (p<0.05) 3. When liver and kidney tissues were observed with optical microscope obvious changes were visible in those tissue.

四君子湯.四君子湯加黃기 煎湯液이 生肌作用에 미치는 影響

안수현,임규상,이기남,An, Su-Hyeon,Im, Gyu-Sang,Lee, Gi-Nam 대한한방안이비인후피부과학회 1989 한방안이비인후피부과학회지 Vol.2 No.1

This study was under taken to investigate the healing of Sa Gun Ja Tang extract (SE) and Sa Gun Ja Tang added Radix Astragali extract (SHE) on the artificial wound on rabbit skin. The granulation tissues were observed by microscope at the five day interval for twenty days. In order to understand the healing mechanism chick embryo culture was carried out in the presence of the extracts and the growth ratio and fusion index were counted. The results obtained were as follows: 1. The healing power of SE treated group on wound was more significant than the control group. 2. The healing power on wound was more effective at the SHE than the SE. 3. The growth ratio and fusion index were higher at the SHE treated groups than the SE treated groups. From the above result, SHE was more effective than the SE on wound healing activity by the mechanism of stimulating the cell growth ratio and fusion index.

한의학의 연구방법론에 관한 고찰

서동진,김정필,이기남,Seo Dong-Jin,Kim Jeong-Pil,Lee Gi-Nam 대한예방한의학회 1997 대한예방한의학회지 Vol.1 No.1