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감송향(甘松香)이 HL-60 세포의 분화유도에 미치는 영향
윤상학 ( Sang Hak Yun ),박종하 ( Jong Ha Park ),김경수 ( Kyeong Su Kim ),오원교 ( Won Kyo Oh ),염승룡 ( Seung Ryong Yeom ),권영달 ( Young Dal Kwon ) 한방재활의학과학회 2007 한방재활의학과학회지 Vol.17 No.1
Objectives: To investigate PKC/MAPKs signaling pathways on the human promyelocytic leukemia HL-60 cell differentiation induced by Nardostachytis Rhizoma (N. Rhizoma). Methods : Differentiation of HL-60 cells was determined through expression of CD11b and CD14 surface antigens using flow cytometry and nitroblue tetrazolium (NBT) assay. PKC activity was measured by using a PKC Kinase Activity Assay kit (Non-Radioactive). Activation of ERK and protein levels of PKC soforms were determined by Western blot analysis. Results : Activation of extracellular signal-regulated kinase (ERK) increased time-dependently in differentiation of HL-60 cells induced by N. Rhizoma. Inhibitor of ERK (PD98059) significantly reduced NBT reduction activity induced by N. Rhizoma in HL-60 cells. In addition, N. Rhizoma increased protein level of PKC α, PKC βI and PKC βII isoforms, but the protein level of PKC γ was constant. PKC inhibitors (GF 109203X, Chelerythrine and H-7) inhibited HL-60 cell differentiation and the ERK activation induced by N. Rhizoma. Conclusions : N. Rhizoma induces granulocytic differentiation via a PKC/ERK signaling pathway in HL-60 cells
독활기생탕가미방(獨活寄生湯加味方)이 배양 척수운동신경세포에 미치는 영향
이영보 ( Young Bo Lee ),윤상학 ( Sang Hak Yun ),임양의 ( Yang Eui Lim ),박진상 ( Jin Sang Park ),정택상 ( Taek Sang Joung ),한경호 ( Kyung Ho Han ),이수경 ( Soo Kyung Lee ),신병철 ( Byung Cheul Shin ),권영달 ( Young Dal Kwon ) 한방재활의학과학회 2003 한방재활의학과학회지 Vol.13 No.4
Objectives & Methods : To evaluate the mechanism of oxidative damage by Xanthine oxidase(XO) and hypoxanthine(HX)-induced oxygen radicals, XTT assay was carried out after the cultured mouse spinal motor neurons were preincubated for 5 hours with various concentrations of XO/HX. And DNA synthesis, PKC activity and c-fos immunohistochemistry were measured to evaluate the protective effect of Dokhwalgisaeng-tang(Duhuojisheng-tang: DGT), Dokhwalgisaeng-tanggami(Duhuojisheng-tangjiawei: DGTG), Herbar Chelidoni(HC) water extract on cultured spinal motor neurons damaged by XO/HX. Results : The results were XO/HX showed the quantitative decrease of DNA synthesis, increase of PKC activity and c-fos positive cell, in proportion to concentration and exposed time on cultured spinal motor neurons and DGT, DGTG and HC showed the protective effects against decrease of DNA synthesis and increase of PKC activity and c-fos positive cell by XO/HX. Conclusions : From the above results, it is suggested that XO/HX have a neurotoxic effect on cultured spinal motor neurons and the herbs extract, such as DGT, DGTG and HC, prevent the toxicity of XO/HX effectively.
박성혁 ( Seong Heak Park ),윤상학 ( Sang Hak Yun ),권영미 ( Young Mi Kwon ),염승룡 ( Seung Ryong Yeom ),권영달 ( Young Dal Kwon ),신병철 ( Byung Cheul Shin ) 한방재활의학과학회 2005 한방재활의학과학회지 Vol.15 No.1
목적 : 진피 (Citus Unshiu, CU)는 실험적으로 항산화, 항알러지, 항종양 효과 등이 있다고 보고되고 있다. 본 연구에서는 비만세포에서 PMA 와 A23187에 의하여 유도된 싸이토카인의 증가에 대한 진피 추출물의 억제 효과와 그 기전을 알아보고자 한다. 방법 : PMA+A23187에 의한 싸이토카인 생성에 대한 진피 추출물의 억제효과 기전을 조사하기 위하여 진피를 처리한 후 IL-1β, IL-8, TNF-α의 생산 및 혈관내피성장인자 (VEGF), 과립구 대식구 군집 자극 인자 (GM-CSF), 저산소 유도 인자 (HIF-1)의 발현을 조사하였다. 결과 : 실험에서 PMA와 A23187을 처리한 경우 대조군에 비하여 IL-1β, IL-8, TNF-α의 생산을 유의하게 증가시켰으나 진피 추출물을 처리한 군에서는 유의하게 억제되었다. 특히 IL-1β의 생성은 103.7±5%, IL-8의 생성은 34.6±7%, TNF-α의 생성은 85.9±4.5% 억제 되었다 (P<0.05). 또한 진피 추출물은 PMA와 A23187에 의하여 증가한 VEGF, GM-CSF, HIF-1α의 발현 증가를 억제하였다 (약 90.9%의 VEGF, 61.6%의 GM-CSF). 결론 : 이러한 결과는 진피가 염증반응에 대한 HIF-1의 억제자로 작용할 수 있으며, 진피가 비만세포 매개성 염증 질환 치료제의 후보 물질이 될 것으로 사료된다.
부자반하탕(附子半夏湯) 추출액(抽出液)이 산소자유기(酸素自由基)에 의해 손상(損傷)된 배양척수운동신경세포(培養脊髓運動神經細胞)에 미치는 영향(影響)
노성일 ( Seong Il Rho ),염승룡 ( Seung Ryong Yeom ),윤상학 ( Sang Hak Yun ),한명금 ( Myeng Gum Han ),정택상 ( Taek Sang Joung ),박진상 ( Jin Sang Park ),한경호 ( Kyoung Ho Han ),권영달 ( Young Dal Kwon ),송용선 ( Yung Sun Song 한방재활의학과학회 2003 한방재활의학과학회지 Vol.13 No.4
Objectives : To evaluate the effect of Bujabanha-tang(Fuzibanxia-tang) water extract on cultured mouse spinal motor neuron which was inhibited by xanthine oxidase/hypoxanthine(XO/HX) Methods and Matrials: induced cytotoxicity, cell viability by the MTT and NR assay and PKC activity, cAMP assay examined after the cultured mouse spinal motor neurons were pre-incubated with various concentrations of Bujabanha-tang(Fuzibanxia-tang) water extract for 3 hours prior to exposure of XO/HX Result : 1. XO/HX, a oxygen radical, decreased the survival rate of the cultured mouse spinal motor neuron cells on NR assay and MTT assay. 2. Bujabanha-tang(Fuzibanxia-tang) have decreasing efficacy of PKC activity increased by XO/HX in cultured mouse spinal motor neuron. 3. Bujabanha-tang(Fuzibanxia-tang) have decreasing efficacy of cAMP amout increased by XO/HX in cultured mouse spinal motor neurons. Conclusions : Bujabanha-tang(Fuzibanxiatang) has marked efficacy as a neuroprotection for the damages caused by XO/HX-mediated oxidative process.
鎭肝熄風湯 및 加味鎭肝熄風湯 추출물이 배양 척수감각신경세포에 미치는 영향
서영석,윤상학,염승룡,이수경,신병철,권영달,송용선 대한동의생리학회,대한동의병리학회 2003 동의생리병리학회지 Vol.17 No.2
To evaluate the mechanism of oxidative damage by Xanthine oxidase(XO) and hypoxanthine(HX)-induced oxygen radicals, XTT assay was carried out. Neurofilament EIA and PKC activity were measured to evaluate the protective effect of Jingansikpung-tang(JST) and Gamijingansikpung-tang(GJST) water extract on cultured spinal sensory neurons damaged by XO/HX, after the cultured mouse spinal sensory neurons were preincubated with various concentrations of JST and GJST water extract for 3 hours prior to exposure of XO/HX. The results were XO/HX decreased significantly, in proportion to concentration and exposed time, the survival rate of the cultured mouse sensory neurons on XTT assay. And in proportion to concentration and exposed time on cultured spinal sensory neurons, XO/HX showed the quantitative decrease of neurofilament by EIA, increase of PKC activity, but JST and GJST showed the neuroprotective effects against decrease of neurofilament and increase of PKC activity by XO/HX. From the above results, it is concluded that XO/HX have a neurotoxic effect on cultured spinal sensory neurons and the herbs water extract, such as JST and GJST prevent the toxicity of XO/HX effectively.
萬金湯 및 加味萬金湯이 배양 척수후근신경절 세포에 미치는 영향
최규선,윤상학,염승룡,이수경,신병철,권영달,송용선 대한동의생리학회,대한동의병리학회 2003 동의생리병리학회지 Vol.17 No.2
The purpose of this study is to examine the toxic effects caused by xanthine oxidase/hypoxanthine (XO/HX) and the effects of herbal extracts such as Mangeum-tang (萬金湯: MGT) and Gamimangeum-tang (加味萬金湯: GMGT) on the treatment of the toxic effects. The results of these experiments were XO/HX, an oxygen radical-generating system, decreased the survival rates of the cultured cells on XTT assay, the amount of DNA syntheses, and the amount of neurofilaments, and increased c-fos positive cells. MGT and GMGT have the efficacy of increasing the survival rates of the cultured cells by increasing the amount of neurofilaments and DNA synthesis and decreasing the c-fos positive cells damaged by XO/HX. From the above results, it is suggested that MGT and GMGT have marked efficacy as a treatment for the damages caused by the XO/HX-mediated oxidative stress. And MGT and GMGT are thought to have certain pharmacological effects.