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강경호(Kyoung-Ho Kang),윤병조(Byung-Jo Yun),어동진(Dong-Jun Euh),백원필(Won-Pil Baek),유선봉(Sun-Bong Yu) 대한기계학회 2006 대한기계학회 춘추학술대회 Vol.2006 No.6
An advanced flow meter applicable to large diameter piping system was developed using the average BDFT(Bidirectional Flow Tube). This flow meter can be used to measure the flow rate of discharged gas having fine floating particles in the stack of the chemical reactor and the large-sized boiler. Also it can be applied to the large-sized industrial piping system having flow of oil without structural instability. As a first step of commercialization, we designed and manufactured the flow meters which are applicable to piping system having diameter of 200㎜ and 500㎜. The calibration test results indicated that this flow meter has linaerity within 0.5% in the Re number range of above 10,000 where is meaningful in terms of application.
백서의 신경병증성 통증모델의 척수감각신경세포에 대한 NMDA 수용체 길항제 및 NOS 억제제의 영향
이강창(Kang Chang Lee),최유선(Yu Sun Choi),이환봉(Hwan Bong Lee),박승택(Seung Taeck Park) 대한통증학회 2002 The Korean Journal of Pain Vol.15 No.1
N/A Background: In order to evaluate the effect of N-methyl-D-aspartate (NMDA) receptor antagonist D-2-amino-5-phosphonovaleric acid (APV), nitric oxide synthase (NOS) inhibitor, and aminoguanidine (AGH) on rat spinal sensory neurons from the neuropathic pain animal model. Methods: Cell viability, amount of neurofilament by immunocytochemistry, and rate of protein synthesis were measured after spinal motor neurons of rats were incubated with various concentrations of APV or AGH for 48 hours. Results: Cell viability of spinal sensory neurons from the neuropathic pain model was remarkably decreased compared with the control. Regarding their protective effect, both APV and AGH significantly increased cell viability, amount of neurofilament and rate of protein synthesis, compared to experimental groups with untreated APV or AGH in spinal sensory neurons. Conclusions: The present study suggests that NMDA receptor, reactive nitrogen species (RNS), and No are involved in neuropathic pain.
FeSO₄로 손상된 골모세포에 대한 五加皮의 영향에 관한 연구
최유선,하대호,정새진,이정헌,김상수,이강창,이환봉,류도곤,박승택 대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.4
In order to evaluate the cytotoxicity of FeSO4 in cultured osteoblasts of neonatal mouse, toxic effect was measured by XTT assay in cultured cells treated with 1∼60uM FeSO4 for 24 hours. And also, the protective effect of Acanthopancis Cortex was examined by cell viability in these cultrures. Cell viability was significantly decreased in a dose- and time-dependent manner after exposure of cultured osteoblasts to 30uM FeSO4 for 24 hours. Protective effect of Acanthopancis Cortex against FeSO4-mediated toxicity was very effective in these cultures. From above the results, it suggests that FeSO4 is toxic in cultured osteoblasts and selective herb extract such as Acanthopancis Cortex iS effective in prevetion of the FeSO4-induced cytoxicity.
Adiramycin이 C6 glioma 세포에 미치는 영향에 대한 원지의 효과
이은미,최유선,손영우,유교상,하대호,이환봉,장철호,이정헌,이강창,이승현,박승택,이건목,류도곤 대한동의생리학회,대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.6
To elucidate the cytotoxic effect of adriamycin on C6 glioma cells, we examined the neurotoxicity induced by adriamycin by MTX assay when C6 glioma cells were cultured in the media containing various concentrations of adriamycin for 72 hours. In addition, neuroprotective effects of herb extract such Polygalae Radix(PR), on adriamycin-induced neurotoxicity in cultured C6 glioma cells were evaluated after C6 glioma cells were preincubated with various concentrations of herb extract, PR for 2 hours before 6uM adriamycin for 72 hours. Adriamycin decreased remarkably cell viability in dose-and time-dependent manner in these cultures, and also herb extract, PR increased cell viability of C6 glioma cells damaged by adriamycin. From these results, it is suggested that adriamycin was highly toxic in cultured C6 glioma cells, and PR was effective in blocking the neurotoxicity induced by adriamycin in these cultures.
Hydrogen Peroxide로 유도된 혈관독성에 대한 杜충의 효과
하대호,최유선,정새진,이은미,이용석,김형수,이환봉,이강창,류도곤,박승택 대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.4
To elucidate the vasculotoxicity of hydrogen peroxide in cultured vascular endotherial cells(VEC), vasculotoxicity was determined by NR assay after VEC was exposed to 5∼20uM hydrogen peroxide for 6 hours. and also, the vasculoprotective effect of Eucommiae Cortex was Measured by NR assay in these cultrures. Cell viability was remarkably decreased dose-dependently, after the treatment with 15uM hydrogen peroxide to cultured VEC for 6 hours. In the vasculoprotective effect of Eucommiae Cortex on the toxicity induced by hydrogen peroxide, Eucommiae Cortex prevented the vasculotoxicity induced by hydrogen peroxide in these cultures. From above the results, it suggests that hydrogen peroxide is toxic in cultured VEC and herb extract, Eucommiae Cortex has protective effect against the vasculotoxicity induced by hydrogen peroxide.