참나무과 2종 삼림토양의 환경요인 및 효소활성도 비교분석

송인근,최영길 ( In Geun Song,Yong Keel Choi ) 한국환경생물학회 1997 환경생물 : 환경생물학회지 Vol.15 No.2

Physicochemical environmental factors and enzyme activities were compared between two forest soils in which different species of family Quercoideae were present. Two species are Quercus mongolica and Quercus dentata. Soil pH were ranged 6.2∼6.6 in both forest soils. Moisture content and total organic matter were 1.4 and 1.3 times higher in the surface soil of Q. mongolica forest than the other, repectively. Cellulase activities at the surface soils were 4.71 times and 2.1 times higher than 25㎝ depth soils of Q. mongolica and Q. dentata forest, respectively. Between the surface soil of two vegetational forest, cellulase activity of Q. mongolica forest was 1.69 times than the other. Cellulase activity was correlated with the soluble sugar (R^2=0.623). Protease acitivity was 1.4 times higher in surface soil of Q. mongolica than the other. But, it was 10 fold higher in 25㎝ depth lower soil of Q. dentata forest than Q. mongolica. Phosphatase activities were similar in 25㎝ depth soils of two vegetational forest. But, phosphatase including acidic phosphatase (ACPase), neutral phosphatase (NEPase), alkaline phosphatase (ALPase) showed higher activities in surface soil of Q. mongolica forest compared to Q. dentata forest. Among the 3 kinds of pH dependent phosphatases, ACPase showed close correlationship with protease (R^2= 0.659).

고온 조건에서 사료 내 생균제의 생존성 및 오염미생물의 생장 억제 효과

김겸헌,이권정,이아란,장인환,송인근,김동운,김수기,Kim, Gyeom-Heon,Yi, Kwon-Jung,Lee, Ah-Ran,Jang, In-Hwan,Song, In-Geun,Kim, Dong-Woon,Kim, Soo-Ki 한국미생물학회 2014 미생물학회지 Vol.50 No.4

여름철의 고온으로의 온도 상승에 따른 사료빈 내의 생균제 L. plantarum, S. cerevisiae 및 B. subtilis의 생존성을 실험실의 가상 조건에서 분석하였다. 상온인 $25^{\circ}C$에서 멸균 사료와 비멸균 사료에 단일 혹은 혼합 균주 첨가 시 pH 변화와 생균제들의 생장을 상호 비교하였다. pH는 멸균 사료와 비멸균 사료 모두에서 4일째에 가장 감소한 것은 같았으나 비멸균 사료에서는 2일째까지는 상승하는 변화의 양상을 보여주었다. 멸균 여부 혹은 혼합 여부와 관계없이 S. cerevisiae와 B. subtilis의 생균수는 일정하게 유지되었지만 L. plantarum의 경우에는 그 수가 모두 줄어드는 것을 확인할 수 있었다. 따라서 3종의 혼합 생균제는 상호 길항작용은 없는 것으로 나타났다. 멸균 및 비멸균 사료에 생균제를 첨가한 후 $60^{\circ}C$의 고온 환경에서 사료의 pH 와 단일접종 생균제의 생존성을 조사하였다. 멸균 및 비멸균 사료 사이에 뚜렷한 pH의 변화는 관찰되지 않았으며 B. subtilis의 pH가 가장 낮게 관찰되었다. 고온 하에서 L. plantarum과 S. cerevisiae 균주는 생존할 수 없었으며, 내열성을 가진 B. subtilis 균주는 생존하면서 사료에 자연적으로 생존하는 오염세균의 증식을 억제하였다. 또 B. subtilis를 접종한 비멸균 사료에서 2일째부터 오염 곰팡이가 관찰되지 않았다. 따라서 내열성이 강한 B. subtilis 균주를 사용하면, 여름철 사료빈 내에 병원성 세균과 곰팡이의 오염을 억제할 수 있는 것으로 나타났다. The aim of this study was to investigate the effect of high temperature on the viability of probiotic organisms (Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae) mixed with animal feed under controlled conditions by simulating a farm feed bin in the summer. Following inoculation of probiotics into the feed, the pH and probiotic viability were monitored during an 8-day incubation at room temperature. Sterile and non-sterile feeds displayed different patterns of pH changes, with increased pH in non-sterile feed at 2 days, but a pattern of decreasing pH at 4 days. The viabilities of S. cerevisiae and B. subtilis after mono/co-inoculation were maintained without substantial changes during the incubation, whereas L. plantarum viability tended to decline. In both non-sterile and sterile feeds, the probiotics were maintained or grew without any antagonistic effects. Probiotic viability was also tested upon a shift to high temperature ($60^{\circ}C$). There was no distinct change in pH between sterile and non-sterile feeds after the temperature shift. L. plantarum and S. cerevisiae could not survive at the high temperature, whereas B. subtilis displayed normal growth, and it inhibited the growth of contaminant microbes. Fungal growth was not observed in non-sterile feed 2 days after supplementation with B. subtilis. Therefore, heat resistant B. subtilis could be safely used in feed bins to inhibit microbial contamination, even at high temperatures. The prevention of elevated temperature in feed bins is necessary for the utilization of L. plantarum and S. cerevisiae during the summer season.

보문 : Pleurotus ostreatus 균사의 생장 촉진 효과를 나타내는 고온성 곰팡이의 특징

이호용 ( Ho Yong Lee ),신창엽 ( Chang Yup Shin ),김준호 ( Jun Ho Kim ),김원록 ( Won Rok Kim ),이영근 ( Young Keun Lee ),장화형 ( Hwa Hyoung Chang ),송인근 ( In Geun Song ),현성희 ( Soung Hee Hyun ),민봉희 ( Bong Hee Min ) 한국균학회 2000 韓國菌學會誌 Vol.28 No.2

DNA 교잡에 의한 토양 미생물 군집의 다양성과 유사성

최영길(Yong Keel Choi),김유영(You Young Kim),송인근(In Geun Song),민병례(Byeong Rye Min),조홍범(Hong Bum Cho) 한국환경생물학회 1999 환경생물 : 환경생물학회지 Vol.17 No.3

To investigate soil bacterial diversity according to vegetation types, directly extracted DNA from 5 different soils were cross-hybridized with each other as a probe and target. Pinus densiflora soil was shown the highest value then agricultured soil>naked soil>grass soil>Quercus mongolicas soil in the order of diversity. Cluster analysis by similarity showed that soil microbial communities were categorized into three groups. [Soil bacterial community, DNA:DNA hybridization, Diversity].

연구논문 : 혼합 생균제 급여가 한국 토종닭의 생산성과 가슴살의 지방산 조성에 미치는 영향

이혁준 ( Hyuk Jun Lee ),김동현 ( Dong Hyeon Kim ),주영호 ( Young Ho Joo ),윤희 ( Hee Yoon ),최인학 ( In Hag Choi ),김수기 ( Soo Ki Kim ),송인근 ( In Geun Song ),장인환 ( In Hwan Jang ),김삼철 ( Sam Churl Kim ) 한국환경과학회 2015 한국환경과학회지 Vol.24 No.3

This study aimed to investigate the effects of dietary supplementation of mixed probiotics on growth performance and fatty acid profiles of Korean native chicken and to provide information regarding producers. Ninety six Korean native chicken (48 males and 48 females, Hanhyup-3-ho) were allocated to 16 wire cages with 4 treatments, 4 replicates and 6 chicks (3 males and 3 females) per wire cage and fed one of four diets containing 0, 0.5, 1 and 1.5% mixed probiotics for 6 weeks. There were no differences among treatments in growth performance of Korean native chicken. For fatty acid profiles, no statistically differences in each fatty acid, total saturated fatty acid and unsaturated fatty acids were observed in all treatments. In the present study, irrespective of statistically differences, inclusion of mixed probiotics tended to improve growth performance, reduce saturated fatty acid and increase unsaturated fatty acids in breast muscles from Korean native chicken compared to controls.

Gamma선 조사에 의한 식품미생물의 살균효과

송인근,최영길,최용 漢陽大學校 環境科學硏究所 1994 環境科學論文集 Vol.15 No.-

한국산 건강식품 가공원료인 알로에, 화분, 효모, 효소 가공분말의 초기 미생물오염도는 각각 8.2× 10²cell/g, 8.7 ×??cell/g, 8.8 ×10⁴cell/g, 6.9× 10⁴cell/g으로 측정되었다. 각 시료군별 감마선 조사를 1∼9kGy로 적용한 결과 초기 미생물의 오염도가 가장 낮은 알로에 분말의 경우 3.5kGy에서 완전 살균이 나타났으며 초기 오염도가 가장 높은 화분 가공분말은 7.2kGy정도에서 완전 살균이 가능하였다. 알로에, 화분, 효모 및 효소 가공분말의 선량 적용에 따른 미생물 생존 곡선에서 ??값은 각각 1.30, 1.20,1.27, 1.20으로 산출되었으며 불활성계수(n)가 6일때 7∼8kGy의 치사선량(??)값을 얻었다. The sample used in this experiment was worked material of health foods manufactured in Korea such as aloe, pollens, yeast prepns. and enzyme prepns. The initial microbial population of above food was 8.2 ×10²cell/g-aloe powder, 8.7 ×??cell/g -pollen, 8.8× 10⁴cell/g-yeast prepns, 6.9 ×10⁴cell/g-enzyme prepns. When samples were irradiated as a serial dose from 1kGy to 9kGy using gamma ray-??, aloe powder showed complete sterilization under 3.5kGy dose. In contrast, complete radio-sterilization in pollens is possible around te 7.2kGy dose of ??-gamma irradiation. ?? value(the dose required to eradicate 90% of the population)presented in microbial suvival curve according to serial irradiation dose was calculated 1.30(aloe), 1.20(pollens), 1.27(yeasts prepns.) and 1.20 (Enzymes prepns), respectively. As a inactivation factor(n) was 6, Lethal Dose(??)is 7∼8kGy dose of gamma irradiation.

Pleurotus ostreatus 균사의 생장 촉진 효과를 나타내는 고온성 곰팡이의 특징

이영근,김준호,이호용,민봉희,김원록,송인근,현성희,장화형,신창엽 한국균학회 2000 韓國菌學會誌 Vol.28 No.3

The mycelial growth of Pleurotus ostreatus in compost is strongly stimulated by solid-state fermentation with thermophilic fungi which were isolated from oyster mushroom compost. The biochemical characteristics of these thermophilic fungi were investigated. Cellulase and ligninase activities were not detected by clear zone effect on CMC and lignin media. All of thermophilic fungi grew well with high mycelial density on xylan media and the growing rate of Sepedonium sp. S-2 observed very high. In results of MUF-test, extracellular enzyme activity of Sepedonium sp. S-2, and S-5 measured very high. On the compost after high temperature fermentation with Sepedonium sp. S-2 and S-5, the mycelial growing rate of Pleurotus ostreatus was increased about 50% and it also showed the inhibiting effect on mycelial growth of Trichoderma sp. SJG-51. Isolated thermophilic fungi, Sepedonium sp. S-2 and S-5 were expected as very useful organism for making oyster mushroom compost.