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유광일,김원록 ( Kwang Il Yoo,Won Rok Kim ) 한국환경생물학회 1997 환경생물 : 환경생물학회지 Vol.15 No.1
Seasonal variation of Chaetognaths in the coastal waters off Kori, was studied. Samples were collected monthly during the period from March 1988 to February 1989 at 30 stations. Ten species belonging to three genera were identified: Sagitta crassa, S. nagae, S. enflata, S. minima, S. regularis, S. robusta, S. neglecta, S. ferox, Pterosagitta draco and Krohnitta pacifica. Mean abundance of chaetognaths changed monthly from 0 to 165 individuals m^-3. The predominant species varied with month; Sagitta crassa occurred abundantly from April to July, S. enflata from August to November and S. nagae occurred throughout the year except for March.
Neutron Dose Response of Tradescantia Stamen Hair Pink Mutations and RBE
Kim,Jin-Kyu,Kim,Won-Rok 대한방사선 방어학회 1998 방사선방어학회지 Vol.23 No.1
자주달개비 4430 클론에 있어서 252Cf 중성자에 의해 유발되는 유전자 돌연변이의 선량반응 관계를 확립함과 동시에 TSH 분홍돌연변이를 유발함에 있어서 X-선에 대비한 중성자의 생물학적 효과비를 산출하였다. 돌연변이를 분석은 공시재료의 방사선조사후 4주 이상 이뤄졌으며 최대변이율을 보이는 조사후 6일부터 13일 까지의 분석결과로 부터 돌연변이빈도를 산출하였다. 가용한 실험범위내에서 중성자 선량의 증가에 따라 돌연변이빈도의 성형적 증가 양상을 나타내었다. X-선에 비교하여 산출한 중성자의 RBE값에 비해 상당히 낮은 값이다. Dose response relationships for one of biological end-points(gene mutation) in somatic cells of Tradescantia 4430 clones were studied using neutrons coming out of a californium-252 isotopic source. And the relative biological effectiveness (RBE) of neutrons in relation to X-ray in the induction of TSH pink mutaions was assessed. Inflorescences were irradiated with X-ray from X-ray generator and neutrons from 252Cf source. Irradiated cuttings were incubated with aeration in neutrient solution under the controlled condition. For more than 4 weeks after irradiation cell mutations were scored. Pink mutation frequencies were calculated from the pooled data for the peak interval (days 6 to 13 post-irradiation). Somatic cell mutations in TSH showed linear dose response relationships in the range of neutron doses available for the experiment. The RBE values estimated for neutrons in relation to X-rays were in the range 3.1 to 6.8 which were much lower than normally recognized value.
Artemia ( Class Crustacea ) 알의 부화에 미치는 이온화방사선의 효과
김진규,김원록,이영근,이창주,장화형 ( Jin Kyu Kim,Won Rok Kim,Young Keun Lee,Chang Joo Lee,Hwa Hyoung Chang ) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.2
Tradescantia in BNL 4430 is a hybrid clone heterozygous for its flower color and extremely sensitive to ionizing radiation. Such a fact made people around nuclear power plants be interested in Tradescantia, a botanical tester of radiation effects. The pur pose of this study is to provide the botanical supplement to physical radiation monitor systems and also to ascertain biological safety regarding environmental radiation around nuclear power plants. Flowers collected periodically from three Tradescantia gardens (in Youngkwang NPP Jeonsigwan. City office and KAERI. respectively) were scored for pink mutation frequencies in stamen hair cells. Pink mutation frequencies in the study area were in the range of 0.82∼1.14. which were slightly lower than the annual mean background mutation frequency of 1.16. Statistical analysis of scoring data showed that there was no significant difference between three Tradescantia gardens (p>0.05). From the result, the environmental radiation level around Youngkwang nuclear power plant can be said biologically safe. Further study for verifying safety will make a role for improving public acceptance related to the peaceful use of nuclear power.
김진규,김원록,이영근,김재성,송희섭,정철영,김기남,서원선,홍광표 ( Jin Kyu Kim,Won Rok Kim,Young Keun Lee,Jae Sung Kim,Hi Sup Song,Cheorl Young Jeong,Ki Nam Kim,One Sun Suh,Kwang Phyo Hong ) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.2
Tradescantia in BNL 4430 is a hybrid clone heterozygous for its flower color and extremely sensitive to ionizing radiation. Such a fact made people around nuclear power plants be interested in Tradescantia, a botanical tester of radiation effects. The pur pose of this study is to provide the botanical supplement to physical radiation monitor systems and also to ascertain biological safety regarding environmental radiation around nuclear power plants. Flowers collected periodically from three Tradescantia gardens (in Youngkwang NPP Jeonsigwan. City office and KAERI. respectively) were scored for pink mutation frequencies in stamen hair cells. Pink mutation frequencies in the study area were in the range of 0.82∼1.14. which were slightly lower than the annual mean background mutation frequency of 1.16. Statistical analysis of scoring data showed that there was no significant difference between three Tradescantia gardens (p>0.05). From the result, the environmental radiation level around Youngkwang nuclear power plant can be said biologically safe. Further study for verifying safety will make a role for improving public acceptance related to the peaceful use of nuclear power.
남극반도 주변 Ice Edge Zone 에서의 하계 모악류의 분포
유광일,김원록,김동엽 ( Kwang Il Yoo,Won Rok Kim,Dong Yup Kim ) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.1
To study the distribution of chaetognaths in Ice Edge Zone near the Antarctic Peninsula during austral summer, samples were collected at 35 stations between Bransfield Strait and northwestern Weddell Sea from December, 1993 to January, 1994. Chaetognaths were consisted of three species, Enkrohnia hamata, Sagitta gazellae and Sagitta marri. The abundance of chaetognaths was 0∼1,359ind./1000㎥ and high values were observed in the northwestern Weddell Sea. Eukrohnia hamata and Sagitta gazellae were distributed widely in Bransfield Strait and northwestern Weddell Sea. The abundance of Eukrohnia hamata was high in northwestern Weddell Sea but it was dramatically dropped in the Bransfield Strait. The abundance of Sagitta gazellae was high in Gerlache Strait and northwestern Weddell Sea and S. marri was very low and showed restricted distribution. The vertical distribution of chaetognaths were mainly confined to 150m and highest density were found in bottom water.
유광일,김원록 ( Kwang Il Yoo,Won Rok Kim ) 한국환경생물학회 1996 환경생물 : 환경생물학회지 Vol.14 No.2
Studies on the spatial distribution of chaetognaths have been carried out on the based of materials collected in the Yellow Sea in Auguest, 1982. As a result, 5 species belonging to two genera occurred; Sagitta crassa, S. enflata, S. nagae, S. regularis and Krohnitta pacifica. A key to the chaetognaths are given. Sagitta nagae was revealed to be a indicator species of the purturbed area formed by the mixing of the warm water current and neritic waters in summer season in the Yellow Sea.
느타리버섯 배지제조 자동화시스템 고체 생물반응기에서의 퇴비화 최적 운전조건 설정에 관한 연구
이호용,김원록,민봉희 대구대학교 기초과학연구소 2001 基礎科學硏究 Vol.18 No.1
This study was conducted to evaluate the optimum processing conditions for composting by aero-thermophilic microorganisms in composting process. Effects of periodic turning and aeration on the composting process using bark and beat-pulp were examined by measuring changes of temperature, moisture content, pH, cell mass and CIN ratio. In the process of composting, the number of microorganisms were counted 10^(6)∼10^(7) CFU/g at 50℃. High temperatures above about 65℃ killed many forms of microbes and limited the rate of decomposition. The composting process with turning gave longer thermophilic stage, higher cell mass and lower C/N ratio, than the process without turning.
방사선을 이용한 느타리 ( Pleurotus ostreatus ) 의 섬유소 분해 변이주 선발
이영근,장화형,김원록,이창주,김진규 ( Young Keun Lee,Hwa Hyoung Chang,Won Rok Kim,Chang Joo Lee,Jin Kyu Kim ) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.2
To isolate the cellulolytic variants, the basidiospores of Pleurotus ostreatus were irradiated at the dose of 1Gy∼20kGy of gamma-ray. After irradiation the germination and survival rates of basidiospores were observed and the activity of extracellular enzyme was determined by means of MUF-substrate method. The 45 variants showed various levels of mycelial growth rate and cellulolytic activity on solid media and vari ous levels of activities of the extracellular enzymes. We have isolated highly cellulolytic variants. 1.5KG-2 and 2KG-2. among the above variants. It is suggested that the isolated 2 variants could be applied usefully to reuse and/or recycle of the cellulosic bioresources.