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이영옥(Young Ok Lee),민봉희(Bong Hee Min) 한국환경생물학회 1999 환경생물 : 환경생물학회지 Vol.17 No.4
To verify the usefulness of enzyme activities as a index for the stability or maturity of organic refuse composting such as grape pomace, Vmax of β-glucosidase, cellobiohydrolase and alkaline phosphatase were measured. The peak values of all measured enzymes at the initial stage of composting were probably associated with easily degradable organic matter in the grape pomace and decreased gradually. But the activities of β-glucosidase and cellobiohydrolase were increased again rapidly whereas that of alkaline phosphatase remained approximately constant after 60 composting days. These results suggest that the increase of enzyme activities during the later periods of grape pomace composting process could be used as a index for their stability. [Vmax, β-glucosidase, cellobiohydrolase, alkaline phosphatase, grape pomace].
대구 지방의 수평면에 입사된 태양 자외선-A의 1998년 후반기 현황
민봉희,서계홍 大邱大學校附設 基礎科學硏究所 1998 基礎科學硏究 Vol.15 No.2
Solar UV-A radiations incident on a horizontal surface at Taegu of Korea in the last half year of 1998 were calculated with 5 minute averages of measurements taken every 30 seconds by a broadband UV-A sensor. Raw signal voltages from the sensor were converted to the units of watt/m2 by the simple linear relationship. The maxima of monthly averages and daily UV-A radiations were observed 412.31 in Aug. and 706.12 kJ/m2/day on Aug. 4, respectively. The maximum of instantaneous UV-A radiations in the period of the observation was 37.22 W/m2 at 12:15 on July 16.
대구지역 대학생의 치아우식 활성과 타액성분에 대한 연구
최수영,민봉희 대구대학교 기초과학연구소 2000 基礎科學硏究 Vol.16 No.2
This study was performed to evaluate the relationship between dental caries and salivary components or properties. Twenty two university students were selected randomly and their salivary factors including viscosity, pH, buffering capacity, and microbiological distribution were analyzed. Among whole case of 22, three students(16.7%) had a moderate activity of dental caries and the rest were caries-free as a result of laboratory tests. The viscosity, acid formation, and the distribution of microorganisms in positive students for dental caries were higher than those in negative students but buffering capacity was low relatively in carious students. In microbiological investigations, there was no significant difference in total bacterial counts between carious and caries-free students. For the standardization and elucidation of dental caries factors, many microorganisms were isolated from carious teeth and identified by using commercial identification kits. Most of the isolated strains belonged to the Genus Streptococcus and identified as Streptococcus agalacoae, Enterococcus faecium and Enterococcus avium by the physiological and biochemical characterization methods. However, the causative agent of dental caries, Streptococcus mutans was not isolated in this study.
韓國産 穀物에서의 trichothecene mycotoxin 生成에 關한 硏究
김학성,민봉희 대구대학교 기초과학연구소 1988 基礎科學硏究 Vol.5 No.-
This study was carried out to investigate the trichothecene mycotoxin productivity of Fusarium tricinctum NRRL 3229. F. tricinctum was cultured on cereal grains at temperature from 10℃ to 15℃ for 4 weeks. Toxins were extracted with solvent and purified partially by column chromatography. The amounts of T-2 toxin, HT-2 toxin, diacetoxyscirpenol and T-2 tetraol were quantitated by gas-liquid chromatography. Corn and millet had the highest productivity of trochothecene mycotoxin. The highest levels of T-2 toxin and HT-2 toxin were produced on corn and maximal yield of T-2 tetraol was obtained in cultures on millet. However, no detectable amount of diacetoxyscirpenol was observed in this experiment. Metabolism of T-2 toxins showed high differences among cereal grains. T-2 toxin was metabolized easily into HT-2 toxin in corn, polished rice, glutinous rice, and sorghum and metabolized very slowly into T-2 tetraol toxin. However, T-2 toxin in unpolished rice was metabolized into HT-2 toxin and T-2 tetraol. Also, T-2 toxin was metabolized rapidly into HT-2 toxin and T-2 tetraol in barley, wheat, millet and red bean while T-2 toxin in unpolished rice was metabolized very slowly into HT-2 toxin and T-2 tetraol.
Purification of Clostridium botulinum type C toxin
Min,Bong-Hee,Choi,Yong-Keel 漢陽大學校 環境科學硏究所 1984 環境科學論文集 Vol.5 No.-
lostridium botulinum type C의 독소 정제법을 확립하였다. Ammonium sulfate 침전법, Sephadex G-100, DEAE-cellulose, QAE-Sephadex, Sephadex G-200을 이용한 컬럼 크로마토그래피로서 고도로 精製된 독소를 얻었다. 이같은 정제방법으로서 얻는 독소는 수율 12.5%, specific toxicity ?? ?? of protein으로 약 1,280배의 정제도를 나타내었다. 정제된 독소는 SDS-polyacrylamide gel 전기영동 및 immunodiffusion test로서 단일물질로 확인되었으며 SDS-polyacrylamide gel 전기영동 결과 정제독소의 분자량은 140,000∼150,000정도였다. Highly purified toxin of Clostridium botulinum type C was obtained by a procedure including ammonium sulfate precipitation. Sephadex G-100 gel filtration, DEAE-cellulose and QAE-Sephadex column chromatography. By this procedure, type C toxin was purified approximately 1,280 folds from the starting material and overall yield of toxin was about 12.5%. The purified toxin was homogeneous in SDS-polyacrylamide gel electrophoresis and double gel diffusion test. The molecular weight of the purified toxin was 140,000∼150,000by SDS-polyacrylamide gel electrophoresis.
대구 지방의 수평면에 입사된 태양 자외선-B의 1998년 후반기 현황
서계홍,민봉희 大邱大學校附設 基礎科學硏究所 1998 基礎科學硏究 Vol.15 No.2
Solar UV-B radiations incident on a horizontal surface at Taegu of Korea in the last half of 1998 were calculated with 5 minute averages of measurements taken every 30 seconds by a broadband UV-B sensor. Raw signal voltages from the sensor were converted to the units of watt/m2 by the simple linear relationship. The maxima of monthly averages and daily UV-B radiations were observed 7.407 in Sep. and 17.462 kJ/m2/day on Sep. 18, respectively. The maximum of instantaneous UV-B radiations in the period of observation was 1.230 W/m2 at 12:45 on July 13.