생체 시료 중 카테콜 아민류, 세로토닌 및 대사물질들의 HPLC/ECD 동시 정량분석

민지현,한영희,Min, Ji-Hyun,Hahn, Young-Hee 대한약학회 2011 약학회지 Vol.55 No.4

Simultaneous monitoring of catecholamines and serotonin with their appropriate extraction from the biological samples is required in order to understand thoroughly the regulation of the central and peripheral nervous system. In the present research the segmented gradient elution with the solid phase extraction using a C18 cartridge rather than the previous isocratic elution with alumina extraction is successfully employed to determine norepinephrine (NE), epinephrine (E), dopamine (DA), serotonin (5HT), 3,4-dihydroxyphenylacetic acid (DOPAC) and 5-hydroxyindoleacetic acid (5HIAA) simultaneously within 20 minutes using 3,4-dihydroxyhydrocinnamic aicd as the internal standard (IS). Linearities were obtained in the concentration range between $5{\times}10^{-6}M$ and $1{\times}10^{-4}M$ for all 7 compounds with detection limits of 0.6~1.9 ${\mu}M$. The present HPLC/ECD method yielded reasonable accuracy (relative error; -1.4~1.1%) and precision (relative standard deviation; 0.4~1.9%) for 9 measurements of the standard solution consisting of NE, E, DA, 5HT, DOPAC and 5HIAA compounds. Recoveries of catecholamines, serotonin and their metabolites from human serum were in the range of 57%~86%. While the concentrations of NE and 5HT in the serum of normal Sprague-Dawley rat were found as $1.4{\times}10^{-6}M$ and $2.6{\times}10^{-6}M$, respectively, the contents of NE and 5HT in the serum of the stressed rat were increased 5.6 times and 1.4 times more, respectively.

SDC계 단실형 고체산화물 연료전지용 SDC-NiO 복합음극의 최적 환원 조건

민지현,안성진,문주호,김주선,Min, Ji-Hyun,Ahn, Sung-Jin,Moon, Joo-Ho,Kim, Joo-Sun 한국세라믹학회 2007 한국세라믹학회지 Vol.44 No.10

We have determined an optimal reduction condition for NiO-based anode in single chamber solid oxide fuel cells that involve samaria-doped ceria (SDC) as an electrolyte. Optimal condition should not only induce sufficient reduction of NiO to Ni, but also prevent the reduction of SDC electrolyte in order to achieve high open circuit voltage (OCV) and power output. Thermodynamic consideration allowed us to determine the optimal anode reduction condition as $96%H_2-4%H_2O$ atmosphere at $250^{\circ}C$. This finding was in a good agreement with the experimental verifications by monitoring the conductivities of SDC and NiO under different reducing conditions.

Effect of Addition of Nano-sized Hydroxyapatite to a Sports Drink on Erosive Potential

민지현 ( Ji Hyun Min ),권호근 ( Ho Keun Kwon ),김백일 ( Baek Il Kim ) 대한구강보건학회 2007 大韓口腔保健學會誌 Vol.31 No.-

증례 : 동기능 부전을 동반한 MELAS 증후군 1예

민지현 ( Ji Hyun Min ),안종화 ( Jong Hwa Ahn ),이정미 ( Jeong Mi Lee ),박정랑 ( Jeong Rang Park ),고진신 ( Jin Sin Koh ),황진용 ( Jin Yong Hwang ),곽충환 ( Choong Hwan Kwak ) 대한내과학회 2013 대한내과학회지 Vol.84 No.2

MELAS 증후군은 비교적 드문 질환으로 비후성 심근증, WPW 증후군, 심장전도장애 등의 심장 관련 증상이 발생할 수 있다. 동기능 이상과 동반된 MELAS 증후군은 거의 알려 진바 없어, 실신을 동반한 동정지로 영구형 심박동기 시술을 시행한 MELAS 증후군 환자 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. A 35-year-old male patient with heart and renal failure and pneumonia was transferred to our department due to recurrent cardiac standstill with syncope. He had been diagnosed as and treated for MELAS (mitochondrial myopathy, encephalopathy, lactic acidosis, and stroke-like episodes) syndrome for the past 3 years. Electrocardiography (ECG) showed the Wolff-Parkinson-White pattern, and an echocardiogram showed hypertrophic cardiomyopathy. He developed syncopal attacks intermittently, and ECG monitoring showed intermittent bradycardia. His Holter monitoring showed several episodes of 5-16 seconds of sinus arrest. We conducted an electrophysiological study to evaluate the arrhythmia. During atrial and ventricular extra-stimuli, cardiac standstill developed several times, and the duration of pauses varied from 2.5 to 5.5 seconds. Abrupt asystolic events also developed accompanying syncopal attacks that were not related to the extra-stimuli. We decided to implant a permanent pacemaker. The patient`s syncopal episodes disappeared after implantation of a DDD type pacemaker. (Korean J Med 2013;84:265-268)

‘통통’ 마늘의 화경제거가 생육에 미치는 영향

민지현(Ji Hyun Min),정재현(Jae Hyun Jeong),한종우(Jong Woo Han),이승주(Seung Ju Lee),정택구(Teak-Gu Jeong) 한국원예학회 2022 한국원예학회 학술발표요지 Vol.2022 No.11

사회복지사의 전문성은 PR커뮤니케이션의 실행과 인식에 어떠한 관련성을 보일까?: 사회복지사들의 인식을 통한 탐색적 연구

민지현(Ji-hyun Min),김수연(Soo-Yeon Kim) 한국광고PR실학회 2018 광고PR실학연구 Vol.11 No.4

이 연구는 173명의 사회복지사를 대상으로 설문을 실시하여 그들의 사회복지사로서의 전문성과 PR커뮤니케이션 실행 능력, 그들이 인식하는 PR커뮤니케이션 인식에 관한 하위 차원을 알아보았다. 연구 결과 사회복지사로서의 전문성이 높은 사회복지사 집단이 전문성이 낮은 집단보다 PR커뮤니케이션 실행 능력이 더 높았다. 그들의 PR커뮤니케이션에 대한 인식 차원은 PR의 주요한 경영적 역할, PR의 이해관계자 관리, PR의 관리자역할, PR의 전략적 목적의 네 차원으로 파악되었으며, 그들의 PR커뮤니케이션에 대한 인식 차원과 사회복자사로서의 전문성과의 관련성을 살펴보았을 때 PR의 이해관계자 관리와 PR의 전략적 목적의 두 차원에 대한 긍정적 인식이 그들의 사회복지사로서의 전문성과 유의미한 정적 상관관계를 보였다. 그들의 인구통계학적 변인들과 관련해서는 사회복지사의 직급, 연령, PR교육 이수 여부, 속한 조직의 SNS운영 여부가 PR커뮤니케이션의 인식과 유의미한 관련성을 보여서 직급과 연령이 높을수록, PR교육을 이수받은 경험이 있을수록, 조직이 SNS를 운영할수록 PR커뮤니케이션을 더욱 중요시했다. 이 연구는 사회복지사들의 사회복지사로서의 전문성과 PR커뮤니케이션에 대한 인식을 함께 살펴봄으로서 사회복지분야에서 가지는 PR커뮤니케이션의 필요성과 의미를 사회복지사로서의 전문성 개념과 함께 탐색해보았다는 데에 의의가 있다. This study explored how social workers’ professionalism relates to their public relations practice and perception on public relations conducting surveys with 173 social workers. Social workers with high professionalism have practiced public relations more than those with low professionalism. Significant management role of public relations, stakeholder management of public relations, management role of public relations, and strategic goal of public relations were found to be significant subdimensions of social workers’ perception on public relations. Social workers with high professionalism and high-ranked position perceived public relations more positively than others with low professionalism and low-ranked position. Elderly social workers and those who have taken public relations training perceived public relations more positively than younger social workers and those who have never taken public relations training. This study is meaningful in that it found the significant implication of public relations in social work.

CAN 및 10Base-T1S 이더넷 통합 차량용통신

민지현(Ji Hyun Min),정소영(Soyoung Jung),박영일(Youngil Park) 한국통신학회 2020 한국통신학회 학술대회논문집 Vol.2020 No.8

예방치과학 분야 : 원저 ; 나노 입자형 수산화인회석을 첨가한 스포츠음료의 치아부식 억제 효과

민지현 ( Ji Hyun Min ),정기호 ( Ki Ho Chung ),권호근 ( Ho Keun Kwon ),김백일 ( Baek Il Kim ) 대한구강보건학회 2007 大韓口腔保健學會誌 Vol.31 No.4

치주 건강 상태에 따른 치면세균막의 산 생성능력 평가에 대한 연구

민지현 ( Ji Hyun Min ),윤홍철 ( Hong Cheol Yoon ),김종관 ( Jong Kwan Kim ),강시묵 ( Si Mook Kang ),김백일 ( Baek Il Kim ) 한국치위생과학회 2015 치위생과학회지 Vol.15 No.2

The aim of this retrospective study was to evaluate the relationship between periodontal health condition and the results of a new method such as Cariview which could evaluate the acidity of dental biofilms. Fifty four subjects more than 20 years old were selected for the candidates of this study. The periodontal health conditions of the candidates were divided into 4 groups according to the assessment of X-ray and Quantitative Light-induced Fluorescence-Digital (QLF-D; Inspektor Research Systems BV) images; gingivitis, slight periodontitis, moderate periodontitis, severe periodontitis. The biofilm acidogenicity of each subject was examined using Cariview (All in ONE BIO) according to manufacturer`s instruction, and the Cariview score was calculated. The mean differences of Cariview score between 4 groups of periodontal health condition were examined by ANCOVA test with the covariance of decayed, missing, and filled teeth (DMFT) index. As a result, the mean Cariview score was different, however it was not significantly different from the 4 groups (p=0.12). The mean score was the lowest in the gingivitis group (40.54±11.01), and the highest in slight periodontitis group (57.26±20.51). In conclusion, the significant mean differences were not confirmed in Cariview score according to the periodontal health condition.

Optical Coherence Tomography를 이용한 초기 치아우식 검사

민지현 ( Ji-hyun Min ) 한국치위생과학회 2016 치위생과학회지 Vol.16 No.4

TMR은 초기우식 평가의 gold standard로 여겨지나 시편을 파괴하여야만 시편의 무기질 밀도를 확인할 수 있는 실험법이다. 그러나 OCT는 비파괴적인 검사법으로 임상에서도 초기우식을 확인하는 데 사용하므로 본 연구에서는 200 μm 이상의 깊은 법랑질 초기우식에서의 OCT와 TMR에서구한 integrated mineral loss값 간의 상관성을 확인하고자하였다. 200 μm 이상의 깊이를 갖는 인공 초기우식병소를 제작하여 TMR (□Z_T_M<sub>R</sub)과 OCT (□R_o_c_t)에서 구한 integrated mineral loss를 각각 구하여 상관성 분석을 시행하였으며 Bland-Altman plot을 그려 두 값 간의 오차 분석을 시행하였다. □R_o_c_t과 □Z_T_M<sub>R</sub간에는 유의한 상관성이 확인 되었으며(r=0.491, p=0.003), Bland-Altman plot 상에서도 □Z_T_M<sub>R</sub과 □R_o_c_t값 간의 차이가 거의 대부분 오차 구간 내에 있는 것이 확인되어 두 측정 방법 간의 오차가 적은 것으로 확인되었다. 따라서 치과임상에서 OCT를 활용하면 초기우식병소의 탐지 및 모니터링 그리고 초기우식병소의 심도 파악이 가능할 것으로 여겨진다. The purpose of this study was to assess the correlation between integrated mineral loss (volume % mineral×μm, ) determined using transverse microradiography (TMR) and integrated reflectivity (dB×μm, □R_o_c_t) determined using optical coherence tomography (OCT) for detecting early dental caries with lesion depth more than 200 μm. Sixty tooth specimens were made from sound bovine teeth. They were immersed in a demineralized solution for 20, 30, and 40 days. The □R_o_c_twas obtained from the cross-sectional OCT image. The □Z_T_M_R was obtained from the TMR image. The correlation between □R_o_c_t and □Z_T_M_R was examined using Pearson correlation. The Bland-Altman plot was constructed using the □R_o_c_t and □Z_T_M_R values. A significant correlation between □R_o_c_t and □ Z_T_M_R was confirmed (r=0.491, p=0.003). Moreover, most of the difference between □R_o_c_t and □ Z_T_M_R was included in the error section of the Bland-Altman plot. Therefore, OCT could be used as a substitute for TMR when analyzing mineral loss in early dental caries.