Vitamin A와 Ethanol이 Dimethylnitrosamine에 의한 Mouse조직내의 DNA, RNA 및 단백질의 손상도에 미치는 영향

김재현,홍연탁,박정식,김승희,강태규,홍성렬,박창원,권오철,이동권,Kim, Jea-Hyun,Hong, Youn-Tak,Park, Jung-Sik,Kim, Seung-Hee,Kang, Tea-Gyu,Hong, Sung-Roul,Park, Chang-Won,Kweon, O-Cheol,Rhee, Dong-Kwon 생화학분자생물학회 1991 한국생화학회지 Vol.24 No.6

Dimethylnitrosamine(DMN)은 간 및 폐, 신장, 식도 등에 앙을 유발시키는 물질이며 정상인의 혈액 및 뇨에서도 0.1~1 ppb정도가 검출되고 있다. DMN은 DNA를 methylation시켜 비정상적인 adduct를 형성하여 암을 유발하게 된다. 본 연구는 ethanol 또는 vitamin A를 mouse에 전처리 하였을 때 $[^{14}C]$으로 표지된 DMN이 생체내의 DNA, RNA 및 단백질과 어느 정도 공유결함을 형성하여 손상을 주는지 측정하였다. 실험결과 DMN은 뇌 또는 췌장에서 보다는 간에 선택적으로 결합하였으며 이 때 간의 DNA와 결합하는 것이 단백질 또는 RNA와 결합하는 것보다 각각 1.4배와 1.75배 높았다. Ethanol을 전처리 하였을 때는 DMN이 ethanol을 투여하지 않은 군보다 간의 경우 RNA와 단백질이 각각 7.5배, 1.4배 정도 더 결합하였다. Ethanol을 전처리한 뇌의 DNA에 6.7배 많이 결합하였으나 RNA에는 오히려 대조군의 15.9% 정도로 크게 감소되었다. 또한, 췌장의 단백질에는 2.0배, RNA의 경우 1.7배 정도 각각 더 결합한 결과를 나타내었다. 반면에 vitamin A 전처리군에서 간 단백질의 adduct는 35% 유의성 있게 감소되었으나 뇌 DNA에서 는 유의성 있게 9.5배 증가하였다. Dimethylnitrosamine(DMN) has been shown to induce tumors of the liver, kidney, esophagus and lung. Furthermore low levels of DMN (0.1~1 ppb) have been detected in normal human blood as well as urine. The purpose of this study is to determine extent of the covalent binding of DMN to DNA, RNA and protein in ethanol- and vitamin A- pretreated mouse. Ethanol or vitamin A was administered for 3 weeks and $^{14}C$-labeled DMN was administered intraperitoneally. Then DNA, RNA and protein were isolated from liver, brain and pancreas and used for determination of radioactivity. DMN was bound selectively to liver DNA rather than brain or pancreas DNA. DNA damage in liver was 1.4 and 1.7 times greater than protein and RNA damage, respectively. Ethanol pretreatment and vitamin A pretreatment increased DNA damage in brain significantly upto 6.7 and 9.5 times, respectively, than the control group.

Vitamin A 와 Ethanol 이 Dimethylnitrosamine 에 의한 Mouse 조직내의 DNA , RNA 및 단백질의 손상도에 미치는 영향

김재현,홍연탁,박정식,김승희,강태규,홍성렬,박창원,권오철,이동권 ( Jea Hyun Kim,Youn Tak Hong,Jung Sik Park,Seung Hee Kim,Tea Gyu Kang,Sung Roul Hong,Chang Won Park,O Cheol Kweon,Dong Kwon Rhee ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.6

Dimethylnitrosamine(DMN) has been shown to induce tumors of the liver, kidney, esophagus and lung. Furthermore low levels of DMN (0.1∼1 ppb) have been detected in normal human blood as well as urine. The purpose of this study is to determine extent of the covalent binding of DMN to DNA, RNA and protein in ethanol- and vitamin A- pretreated mouse. Ethanol or vitamin A was administered for 3 weeks and ^(14)C-labeled DMN was administered intraperitoneally. Then DNA, RNA and protein were isolated from liver, brain and pancreas and used for determination of radioactivity. DMN was bound selectively to liver DNA rather than brain or pancreas DNA. DNA damage in liver was 1.4 and 1.7 times greater than protein and RNA damage, respectively. Ethanol pretreatment and vitamin A pretreatment increased DNA damage in brain significantly upto 6.7 and 9.5 times, respectively, than the control group.

소셜커머스 이용 후 재구매의도의 영향요인에 관한 연구

김재현(Kim, Jea-Hyun),정대율(Jeong, Dae-Yul),조성의(Cho, Sung-Eui) 한국전자상거래학회 2013 전자상거래학회지 Vol.14 No.1

본 연구는 소셜커머스의 재구매의도에 영향을 미치는 요인을 검정하기 위해 탐색적 연구로 진행하였다. 선행요인은 흥미성, 고객맞춤화, 거래용이성, 거래보증성, 보안성, 반응성 등을 선택하였으며, 소비자행동 분야에서 빈번하게 연구되어져온 인지부조화 이론을 참고하여 인지부조화 요인의 조절효과를 검정하였다. 인지부조화의 조절효과 검정은 위계적 회귀분석(hierarchical multiple regression analysis)을 이용해 상대적 영향력 평가(베타(β)값)를 하여 재구매의도에 가장 큰 영향력을 가진 선행요인을 발견하였다. 분석결과 고객맞춤화를 제외한 흥미성, 거래용이성, 보안성, 거래보증성, 반응성 등이 재구매의도에 유의한 영향을 미쳤다. 상대적 영향력 평가결과 거래용이성이 표준화 계수인 베타(β)값의 절대치 0.259로 가장 높게 나타났으며, 다음으로 반응성(0.205), 흥미성(0.191), 거래보증성(0.174), 보안성(0.101) 등의 순으로 나타났다. 조절효과 검정결과 고객맞춤화와 거래보증성에서 인지부조화의 조절효과가 있는 것으로 나타났다. 이러한 실증분석결과는 고객의 구매 후 인지부조화 상태를 고려하여 요인 간 영향관계를 설명하고 있다는 점에서 의미가 있다. 또한 소셜커머스의 재구매의도 결정요인의 상대적 영향력과 요인간의 영향관계를 검정하였다는 점에서 의미가 있으며, 관련실무자들이 마케팅전략을 설정하는데 있어 시사점을 제공할 수 있다. This study was performed to investigate factors affecting re-purchase intention in social commerce. Independent factors selected in this study were enjoyableness, customization, ease of use, assurance, security, and responsiveness in social commerce. In addition, cognitive dissonance was adopted as a moderating factor in the relationship between independent factors and re-purchase intention. Hierarchical multiple regression analysis was adapted to analyze the relationship among adopted factors. The results of the analysis showed that all factors except for customization affect significant on re-purchase intention. In addition the moderating effect of cognitive dissonance existed in the relationships between customization, assurance and repurchase intention. The results explains that the post-purchase cognitive dissonance can do a role in the relationship between independent factors and repurchase intention in social commerce. This study is also meaningful in that the results explains significant effects of independent factors reflecting service characteristics on repurchase intention in social commerce. The results of this study can provide implications on practitioners who are involved in marketing strategy building in social commerce.

스마트폰 사용자의 보안수칙 실천 부족에 관한 연구: 효능감의 역할을 중심으로

김재현 ( Jea Hyun Kim ),김종기 ( Jong Ki Kim ) 한국정보시스템학회 2015 情報시스템硏究 Vol.24 No.3

Purpose This study discusses the contradictory behavior of smart-phone users who consider security is important, but they do not follow the security recommendations. We found through literature research that this contradictory behavior is resulted from a low level of efficacy. Design/methodology/approach Research hypotheses were set based on Extended Parallel Process Model, Control Theory, and Self Efficacy Mechanism. The data were collected from undergraduate students. Total of 178 data were used for the analysis. Findings Results of the analysis, first, showed that the relationship between threat and security attitude varies with the level of coping efficacy. Second, showed that the relationship between threat and fear does not vary with the level of coping efficacy. Both the groups with high coping efficacy and low coping efficacy had a statistically significant effect on the relationship between threat and fear.

수종약물이 Dimethylnitrosamine에 의한 DNA, RNA 및 단백질 손상도에 미치는 영향

김재현(Jea Hyun Kim),박정식(Jung Sik Park),홍성렬(Sung Ryul Hong),권오철(O Cheul Kweon),박창원(Chang Won Park),이동권(Dong Kwon Rhee) 대한약학회 1991 약학회지 Vol.35 No.6

The purpose of this research is to evaluate effects of chloramphenicol, phenobarbital and progesterone on damage of DNA, RNA and protein which was induced by dimethylnitrosamine. N,N-Di[14C] methyl-nitrosamine (DMN) was used as a damaging agent and levels of DNA, RNA and protein damage in liver, brain and pancreas were compared with a control group. Pretreatment of mice with chloramphenicol increased protein damage in pancreas two times more than the control level. Liver RNA damage was increased up to 5.8 times and brain DNA damage up to 6.95 times by treatment of phenobarbital but brain RNA damage was decreased significantly down to 21% of the control group. The damage of liver RNA was significantly decreased by treatment of progesterone, although liver protein damage, pancreas RNA damage and pancreas protein damage were increased.

소셜커머스의 서비스특성 요인과 재구매의도

김재현(Kim, Jea Hyun),조성의(Cho, Sung Eui) 한국서비스경영학회 2013 서비스경영학회지 Vol.14 No.1

Social commerce is a new business model intermediating grouped customers and service providers in electronic commerce. The proportion of social commerce in electronic commerce sales is increasing sharply in recent years. In this study, factors affecting customer purchase intention in social commerce were examined and the role of ``trust`` in the relationship was explored. From the result, three independent factors of ``ease of transaction``, ``service assurance`` and ``responsiveness`` reflecting service characteristics in social commerce had significant effect to customer attitude and purchase intention, and ``trust`` did important role in the relationship. Data for this study were collected through surveys on customers experienced social commerce transaction and structural equation modeling methodology was adopted for the analysis.

소셜커머스에서 고객의 구매 결정요인 연구

김재현(Jea-Hyun Kim),조성의(Sung-Eui Cho) 한국인터넷전자상거래학회 2014 인터넷전자상거래연구 Vol.14 No.1

This study attempts to identify the factors influencing customer purchase intention in social commerce. Six independent factors such as service quality, customer profit, reliability, security, firm"s reputation, and social influence were extracted and the relationships were explored. This study was carried out through customer survey and statistical analysis on the result of the survey. Multiple regression analysis was adopted for the analysis. From the result of the analysis, four independent variables such as customer profit, reliability, security, and social influence significantly affected customer purchase intention in social commerce. In addition, the average value of customer profit and coefficient of social influence were relatively high showing that these two were the most important factors affecting customer purchase intention in social commerce. However, service quality and firm"s reputation did not have significant influence on customer purchase intention showing different result from the cases of traditional electronic commerce. The results of this study are meaningful in that factors affecting customer purchase intention in social commerce were identified from comprehensive perspective and the importance of customer profit and social influence was verified through empirical study in social commerce.

젊은 성인의 폐기능 분석과 예측식 연구

김재현 ( Jae Hyun Kim ),최진형 ( Jin Hyung Choi ),조인기 ( In Ki Cho ),정화정 ( Hwa Jeoung Jeong ),허은지 ( Eun Ji Heo ),임승범 ( Suang Bum Lim ),신다혜 ( Da Hye Shin ),윤의정 ( Eui Jeong Yoon ),이지선 ( Ji Sun Lee ),이재인 ( Jea 대한신경치료학회 2012 신경치료 Vol.16 No.1

Purpose The purpose of this study was carried out to determine the normal predictive equation of pulmonary function and related factors in Korean young adult(university students) using spirometer. Methods Measured the FVC (Forced Vital Capacity), FEV 1 (Forced Expiratory Volume in one second), ERV(expiratory reserve volume),VC(vital capacity) and surveyed body height, body weight. years. Study group was total I02(male 57, female 45)who were sampled from healthy young adults(Age is 18-28) at the shinsung university in dang gin city. Result The results of this study were summarized as follows: (I) FVC in male and female was 4.6±0.6 L. 3.3±OA L. FEV1 in male and female was 3.9±0.5 Land 2.7 ±OAL. (2) In correlation analysis, FVC, FEV1 showed positive correlation with body height body weight and negative correlation with age. (3) The prediction equation for FVC was 0.077 X height(cm)+0.020 X years +0.002 x weight-9.387 in male, and 0.030xheight(cm)+0.023xyears+0.006xweight-2A68in female. (4) The prediction equation for FEV1 was 0.055 x height( cm) -0.007 x years +0.003 x weight-5.855 in male, and 0.32 x height( cm) +0.007 x years -0.0 11 x weight (kg)-2.029 in female. Conclusion In conclusion, these results suggest that body height, body weight have significant association with pulmonary function. More research is need in the further for using these parameters in predictive equation.

RSSI를 이용한 향상된 TDOA와 Pattern Matching 간의 선택적 실내 측위에 관한 연구

허수정,김재현,박용완,Hur, Soo-Jung,Kim, Jea-Hyun,Park, Yongwan 대한임베디드공학회 2013 대한임베디드공학회논문지 Vol.8 No.1

This paper researches location estimating method in CDMA system. Previously proposed positioning algorithms are difficult to estimate accurate position in indoor environments, and possible to limited position. This paper proposes enhanced algorithm using received PN pilot signals from base stations to enhance previous algorithms. For estimating position, we set the threshold value and use over the threshold value in received signals. After selecting signals, we estimate position using TDOA algorithm. And the cases which TDOA algorithm cannot use to estimate position, we use Pattern Matching algorithm. The proposed method system showed the improved performance in estimating parameters and locating positions by computer simulations.

사회적 자본과 개인 동기가 정보공유와 커뮤니티 촉진에 미치는 영향: 중국의 가상커뮤니티를 중심으로

김종기 ( Jong Ki Kim ),대상 ( Shuang Dai ),김재현 ( Jea Hyun Kim ) 한국정보시스템학회 2016 情報시스템硏究 Vol.25 No.1

Purpose Virtual communities change the way people communicate and share information. The purpose of this paper is to find how internet social capital and individual motivations influence the information sharing in virtual communities. This study considers the social capital theory, individual motivations, information sharing, and community promotion to construct a theoretical model. Design/methodology/approach Social capital focuses on three dimensions that include 6 factors: social interaction ties, centrality, shared language, shared vision, trust and reciprocity. Individual motivations include 2 factors: reputation and enjoy helping. To confirm the research model and the hypotheses, 426 effective questionnaires were used for the final analysis. Findings The result of data analysis demonstrates that social interaction ties, centrality, shared language, trust, and reciprocity were significant in affecting information sharing behaviors. However, reputation, enjoy helping, and shared vision does not appear to have a significant influence on information sharing behaviors. The information sharing was positively related to community promotion.