흉요추부 방출성 골절의 척수강 복원에 대한 방사선학적 고찰

이준규,오기영,김형성 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.2

We analyzed 44 cases of burst fractures on the thoracolumbar junction which were operated with posterior instrumentation technique at the Department of Chungnam University Hospital from 1988 to July 1995. Reduction of the spinal fragment through kyphosis correction and distraction were evaluated using conventional radiographs and CT imaging between postop., POD 6 months and POD 12months in 23 cases of burst tractures. The purpose of this study was to evaluate the spinal canal remodeling using radiographic means. The result were as follows : 1. The average kyphotic correction angle was 7.4˚ and there was no correlation between the reduction of retropulsed fragments and the amount of kyphotic angle correction. 2. There was significantly correlation between the amount of reduction of posterior vertebral body and amount of retropulsed fragment. 3. There was no correlation between the degree of canal compromise and the Denis type of thoracolumbar burst fractures. 4. There was significantly correlation between the shape of the patent portion of the spinal canal and neulologic deficits.

다도해 상록활엽수림 대표식물군락의 토양환경과 미생물군집과의 상관관계

李吾炯 木浦大學校 沿岸環境硏究所 1992 沿岸環境硏究 Vol.9 No.1

To analysis the stability of the soil ecosystem, fertility of soils, and the extent of biochemical operation performed by soil microorganisms, the soils of the five representative communities of Camellia japonica, Machilus thunbergii, Castanopsis cuspidata var. siebodii, Quercus acuta and Quercus myrsinaefolia of the evergreen broad-leaved forests of Dadohae National Marine Park were investigated for the soil properties, population size of soil microbes, and correlation between them. Investigations about soil properties and microbial populations of above 5 representative communities revealed that the soil of M. thunbergii community was relatively rich in total nitrogen, organic matter, and phosphorous, and most high in microbial populations. The correlation coefficient between soil properties and microbial populations also showed that soils of M. thunbergii community was ecologically most stable, in that they were uniformly high in all parameters investigated. The total nitrogen content was turned out to be the most important environmental factor for the evergreen broad-leaved forests of Dadohae National Marine Park, since the correlation coefficient between it and microbial populations was highest.

분극세기 및 열에이징이 PZT 세라믹스 길이진동모드에 있어서 온도안정성에 미치는 효과

오형철,현덕수,이개명 濟州大學校 情報通信硏究所 2000 情報通信硏究所論文集 Vol.3 No.-

The length extensional vibration mode of a piezoelectric ceramic resonator has the advantage of reduction in its size. In this paper. PZT piezoelectric ceramic specimens with three compositions (Zr/Ti = 56/44, 53/47, 50/50) in Pb(Zr_xTi_(1-x))O₃system were fabricated. We studied effects of poling strength and thermal aging on the vibration mode of the specimens which were poled at 1.5, 2.5, 3.5[KV/㎜] respectively and thermally aged for an hour at 200℃ respectively. The electro-mechanical coupling factor of the length extensional vibration mode increased as poling strength increased. and it decreased after thermal aging. The temperature coefficient of resonant frequency decreased in the 56/44 specimen as poling strength increased after thermal aging. but it increased in the 50/50 specimen. On the conclusion. the changes of poling strength effected on the piezoelectric efficiency and thermal aging contributed to the stabilization of electric dipole structure of the 56/44 specimen.

영산호의 미생물 분포와 환경요인과의 상관관계

이오형,송태곤 木浦大學校沿岸生物硏究所 1987 沿岸生物硏究 Vol.4 No.1

영산호의 생물학적 수질조사 결과 타가영양세균은 표층, 저층 모두 1985년 7월에, 대장균류는 표층, 저층 모두 1986년 9월에 최대치를 나타내었으며 정점 별로는 정점7과 정점3이 모두 최대치를 나타내었다. 미생물개체군의 7정점 평균밀도는 타가영양세균의 경유10³∼10 ( )cells/㎖ 범위였고 대장균류는 10²∼10⁴cells/100㎖ 여서 영산호는 부영양화 내지 과영양화상태로 오염된 수도 3급수 지역임이 판명되었으며 환경요인과의 상관관계 조사에서 미생물개체군의 크기가 수온과 BOD에 가장 높은 관계를 나타내 영산호의 주된 오염물질은 BOD성 유기물질이며 이들은 주로 영산호 상류(정점7)와 영암족(정점4)에서 유입되고 있는 것으로 추론되었다. Microbial distribution and its relationship to the enviromental factors of Yongsan Lake were investigated at 7 stations from July, 1986 to May, 1987. The microbial distribution in the lake showed high variability depending on the seasons and stations. Average number of heterotrophic bacterial in surface layer was 0.52×10⁴cells/㎖ and in bottom layer, 2.63×10⁴cells/㎖. In surface layer and bottom layer of the lake, the average number of coliform bacteria was 1.12×10³cells/100㎖ and 2.32×10³cells/100㎖, respectivly. Average number of heterotrophic and coliform bacteria of investigated stations were 10³∼10( )cells/㎖ and 10²∼10⁴cells/100㎖, respectively. These results indicated that Yongsan Lake was eutrophicated. The microbial density and distribution were closely related with water temperature and BOD. The causative material of pollution was inferred to be organic matter and allochthonous sediment.

AGV와 作業物에 대한 日程計劃

李永海,吳瀅述 漢陽大學校 産業經營硏究所 1989 産業經營硏究 Vol.1 No.-

Which machine processes the first operation of some jobs will affect the subsequent job scheduling under automated manufacturing system environment with alternative routings allowed. This study presents the heuristic algorithm which can decide the routings in consideration of alternative machines in the case of two alternative machines.

한국 미생물 자원의 개발연구 : 특히 유용 미생물주(균주)의 분리와 응용에 관한 연구 The Research on the Isolation and Application of Utilizable Microorganisms Related to Jeonju Materials

이주식,백승화,이오형 Natural Science Research Institute JEONJU UNIVERSI 1987 전주대학교 자연과학연구소 학술논문집 Vol.2 No.2

The isolated strains of microbes from some hay, water of lake, marketing milk and Tachju brewing in Jeonju area. Were as follows Isolated 10 strains of Bacillus group, 2 strainins of Chlorella group, 2 strains of Lactobacillus group, 2 strains of Saccharomyces grpup and other microbes and determinated 4 strains in the 20 strains and application of utlizable microorganisms studies on the strains or pure cultual materials. Microbiological and biochemical examined for each organism. Its Bacillus species, Lactobacillus species, Chlorella species and Saccharomyces species were as follows. Bacillus species in Bacillus subtilis natto like strain. In addition, some of these strains were prepared to show different responses to the biotin added medium. Therefore our primary purpose was to examine the possible effects of glutamic acid, biotin and boiling soybean extract on the growth or sporulation of one these strains. It is a novel finding that biothin and soybean extrat affects on its sporulation. Glulamic acid was required for both its growth and sporulation. Chlorella species is Chlorella ellipsoidea like strain. The optimum cultural conditions and composition of the medium for wild Chlorella ellipsoidea were studies as follow. The addition than in control in concentration of the nitrogen sources high presented the effective result in the increasing order of meat extract, peptone amino, acid. Lactobacillus species is Lactobacillus casei like. Lactobacillus casei was isolated relatively high temperature and high salt contend and mesothermophilic condition. In addition, among, J-1, J-2, J-3 strains isolated together from above samples, J-2 was prominant in producing typical curd within 20 hours. Saccharomyces species is Saccharomyces cerevisiae like. When Sacchromyces cerevisiae like was used as a sole yeast source, this was dominated soon after it. But in this case Hansennula, Candida, Pichia, Torulopsis etc. were alse found out. This fact indicates that many kinds of yeast species were related with the alcohol fermentation. Biochemical studies on Cell-bound a-amylase in Bacillus subtilis like. Extracelluiar and Cell-bound a-amylase were purified separately and some of their biochemical properties examined. The amount of extracellular a-amylase produced by bacteria is known to very greatly with growth conditions. Extracellular a-amylase activity was more inhibited than cell bound a-amylase by metal ions tested.

중합효소연쇄 반응법에 의한 인형거대세포바이러스(human cytomegalovirus)의 신속한 검출

김의종,오명돈,박기호,신형식,이환종,김남중,최강원 대한감염학회 1996 감염 Vol.28 No.4

목 적 : 인형거대세포바이러스(HCMV)는 면역 저하 환자에서 폐렴, 망막염, 간염 등 치명적인 감염증의 원인이다. 전통적인 세포 배양법으로 HCMV를 분리하기까지는 1주에서 4주가 걸리므로 이 바이러스에 의한 질환이 의심되는 경우 신속한 진단법을 이용한 진단이 바람직하다. 저자들은 한국에서 분리되는 야생주 HCMV를 검출하는데 중합효소 연쇄 반응법을 이용할 경우의 진단적 유용성을 알기 위하여, 전통적인 세포배양법으로 HCMV가 분리된 검체를 이용하여 중합효소 연쇄 반응법의 특이도와 민감도를 평가하였다. 방 법 : 국내 야생주 HCMV 15주, 그리고 전통적인 바이러스 배양법으로 HCMV가 분리된 소변 15검체를 대상으로 중합효소 연쇄 반응을 실시하였다. 중합효소 연쇄 반응은 Towne주의 immediate early antigen의 유전자에서 유리된 primer MIE와 AD169주의 immediate early antigen의 유전자에서 유리된 primer IE를 이용하였다. 증폭 산물은 겔 전기영동 후 EtBR으로 염색하여 자외선 하에서 관찰하였다. Primer IE에 의한 증폭산물은 또한, DNA blot hybridization 방법으로 확인하였다. 결 과 : 1) 국내 HCMV 야생주 15주를 대상으로 중합효소 연쇄 반응을 한 결과 primer IE를 이용한 경우에는 100%(15/15)에서 177bp의 증폭 산물이 관찰되었고, 이들 증폭 산물은 모두 probe IE와 보합 결합되었다. primer MIE를 이용한 경우에는 93%(14/15)에서 435bp의 증폭 산물이 관찰되었다. 2) 세포 배양법으로 HCMV가 분리된 소변 15검체를 대상으로 primer IE를 이용하여 중합효소 연쇄 반응을 한 결과 direct gel analysis 법으로는 73%(11/15)에서, 보합 결합법으로는 87%(13/15)에서 관찰되었다. Primer MIE를 이용한 경우에는 direct gel analysis법으로 87%(13/15)에서 435bp의 증폭 산물이 검출되었다. 결 론 : Primer IE와 MIE를 이용한 중합효소 연쇄 반응법은 국내 야생주 HCMV를 신속히 검출하는데 유용한 검사법이다. Background : Human cytomegalovirus(HCMV) can cause pneumonitis, hepatitis, retinitis and other serious diseases in the immunocompromised patients. It takes 1 to 4 weeks to diagnose HCMV infection by conventional virus culture. Therefore, when HCMV diseases are suspected, a rapid diagnostic method such as polymerase chain reaction(PCR), antigen assay or shell vial culture is desirable. We evaluate the sensitivity and specificity of a PCR for the rapid detection of HCMV wild strains in Korea. Methods : We used 2 sets of primers ; primer IE and primer MIE derived from the sequence for immediate early gene of AD169 strain and Towne strain, respectively. Fifteen clinical isolates of HCMV, suspended in MRC-5 cells, were amplified by PCR. Fifteen urine specimens which were positive for HCMV by conventional virus culture were also amplified. Amplification products were analyzed by polyacrylamide gel electrophoresis. The products from PCR with primer IE were also identified by DNA blot hybridization. Results : PCR using primer IE gave the PCR products in all of the 15 HCMV wild strains. All of these were hybridized with probe IE. When primer MIE were used, 93%(14/15) of the wild strains showed amplified bands by direct gel analysis. When the urine specimens were amplified by PCR with primer IE, amplified bands were seen in 73%(11/15) by direct gel analysis ; 87%(13/15) by hybridization method. When primer MIE were used, 87%(13/15) of the urine specimens showed the PCR products by direct gel analysis. Conclusion : Polymerase chain reaction with primer IE and MIE may be a specific and sensitive diagnostic method for rapid detection of HCMV wild strains in Korea.

소흑산도의 토양미생물과 해양미생물의 환경적 분포

임병선,이오형,이건형 木浦大學校沿岸生物硏究所 1988 沿岸生物硏究 Vol.5 No.1

The physicochemical properties of soil and the distributions of both soil and marine marine microorganisms of Sohuksan-do, Chonnam were investigated on 22th of May, 1988. The results were as follows: 1. The physicochemical properties of soil 5. investigated sites revealed their pH values ranged 5.0-5.9(mean, 5.52). The values of electroconductivity, organic matter, total nitrogen, and water content were in the range of 140-190 ㎛ho/㎝ , 9.8-18.8%, 1.25-3.62㎎/g , and 27.2-46.5%, respectively. 2. Distribution of soil microbes The total count of bacteria, fungi, and actinomycetes were in the range of 3.0 -8.8 ×10??cells/g soil, 0.7-1.5 ×10??cells/g soil, and 2.0-2.8 ×10?? cells/g soil, respectively. Bacteria occupied about 64.2% of total population, and fungi 9.9%, actinomycetes 26.0%, respectively. Of the total count of each group, cellulolytic bacteria occupied about 13.7% of total bacteria, and celulolytic fungi, about 49.8%, cellulolytic acvtino-mycetes, about 12.2%, respectively. It was worth noting that cellulolytic fungi occupied a large proportion of the total count of fungi, meaning active progress of cellulose decomposition in this area. 3. Distribution of marine microbes The popolarion density of marine bacteria was found to be in the range of 1.07 -4.77 ×10??cells/㎖ at the surface layer, 1.02-7.77 ×10??cells/㎖ in the middle layer, and 2.53 6.58 ×10??cells/㎖ at the bottom layer, respectively. These results showed tendency of higher density at the euphotic zone. In caseof physiologically characteristic bacteria, the population density of proteolytic bacteria was higher than that of either lipolytic or amylolytic bacteria, meaning independence of this area from incorporation of nutrients from outside.

심실보조장치 이식의 새로운 수술기법-Korea University Technique-

선경,박성영,오혜정,신재승,이혜원,심환주,김형묵 제주대학교 인공심장이식연구소 2001 인공심장 연구 Vol.2 No.1

고려대학교 흉부외과에서는 심실보조장치 이식에서 흉골 재절개(re-sternotomy)에 따른 합병증과 사망률을 감소시키기 위하여 우측 개흉을 이용한 독자적인 수술법을 개발하였다 (KU technique). 다양한 동물모델과 사체실험 및 임상적용을 통해 안정성과 유용성을 확인하였으며, 향후 좌심 및 양심보조장치 이식에서 표준기법의 하나로 제안하고자 한다. We have developed a new surgical technique (KU technique) of right thoracotomy for cannulation of ventricular assist devices, which can lessen potential morbidity and morta1ity from re-sternotomy. The technique has been proved to be safe and efficient in various animal models, preclinical cadaver fitting test, and human application. We would like suggest KU technique as a part of standard in implantation of left ventricular or hi-ventricular assist devices.

국소성 피질이형성증의 외과적 치료

조관국,김형일,이민철,김근수,김연희,정창오,Neto, Pedro R. 대한신경외과학회 1996 Journal of Korean neurosurgical society Vol.25 No.12

Corticai Dysplasia(CD) is increasingly recognized as a cause of intractable epilepsy since it can be easily diagnosed with high resolution magnetic resonance imaging(MRI). We analyzed 24 consecutive cases with cortical dysplasia who underwent resective surgery at our institute between September. 1992 and December. 1995. MRI was demonstrated to be a decisive tool to identify CD in 16 cases(66.7%). However, the remaining 8 cases(93.9%) were confirmed to have CD based on histological examination. Location of the lesion included temporal(n=9), central(n=5), multilobar(n=5), and frontal(n=5). Intracranial EEG recording was performed to delineate the epileptogenic zons in 16 cases : subdural grid(10 cases) and depth electrode(6 cases). Resection was performed in temporal(n=9), frontal(n=7), central(n=5). parietal(n=1), and muitilobar(n=1). Complete resection was possible in 15(62.5%) and partial resection in 9(37.5%). Histological examination revealed dyslamination of cortical layers only(n=9), additional dysplastic neurons(n=7), and additional balloon cells(n=8). Surgical outcome was graded as seizure free(n=14 : 58.3%), rare seizures(n=3 : 12.5%). <50% reduction of seizure frequency(n=2 : 8%), and no change(n=5 : 20.8%) after mean follow-up of 12.7 months. The most influencing factor on the surgical outcome was the degree of completeness of resection(p<0.05). However. detectability of CD on MRI, severity of histological findings, age of seizure onset, and duration of seizure were not significantly related with the surgical outcome High suspicion is required to detect the CD among the patients with intractable epilepsy and surgical strategies should be well-planned to improve the surgical outcome in the patients with CD.