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      • Fast and sensitive fluorescent detection of organic mercury species using fluorescent probe based on arylboronic acids

        이건형 한국공업화학회 2020 한국공업화학회 연구논문 초록집 Vol.2020 No.-

        Aryl boronic acids that were used as important building blocks in organic synthesis have been widely employed in carbohydrate detection, H2O2 sensing, protease enzyme inhibitors, anticancer agents, and drug delivery systems. We present a new fluorescent probe (1) based on the fast displacement reaction of arylboronic acid with inorganic and organic mercury species in purely aqueous solution and live cells. 1 recognized Hg2+ and methylmercury by turn-on responses through the irreversible chemical reaction of the arylboronic acid moiety with the mercury species. 1 showed promising sensing properties for mercury ions such as low detec-tion limit (1.82 nM, 0.36 ppb), high selectivity and sensitivity, fast reaction (< 2 mins), and operation in purely aqueous solutions and live cell.

      • KCI등재

        중국 모바일 상거래 e-고객니즈관리에 관한 탐색적 모형 연구

        이건형,후앙야오야오,시에린펑 국제e-비즈니스학회 2019 e-비즈니스 연구 Vol.20 No.6

        This study aims to explore measurement model of e-customers management for improving the quality of e-commerce company's customer needs management in the perspectives of e-consumers. The questionnaire was developed through literature review on customer needs management of mobile e-commerce, and online survey was conducted to verify whether the collected data is suitable for the proposed predictive model by applying the PLS analysis method which is suitable for new variable development. The valid samples of 99 Chinese people who are engaged in purchasing activities using only mobile e-commerce were extracted, and a questionnaire consisting of 20 items was produced as an analysis tool for analyzing the e-customer needs management measurement model. The collected data were analyzed using SmartPLS 3.0. As a result, the first-order constructs of understanding customer needs, identifying customer needs, customer response, and customer communication that constitute e-customer needs management are represented as reflection indicators (mode A). The CTA-PLS approach verified a reflection-formative type Ⅱ model that consists of formative indicators (mode B) that predict perceived e-customer needs management. By presenting data-based empirical data from the perspective of e-customer marketing that affects the purchasing behavior of Chinese e-commerce, this study contribute to provide basic reference data for constructing a structural model that can identify the relationship of e-customer needs management 본 연구는 중국내 모바일 전자상거래를 이용하여 구매하는 e-고객이 소비자 측면에서 전자상거래 업체의고객니즈관리에 대한 질적 향상을 위한 e-고객니즈관리 측정 모형을 탐색하고자 하였다. 모바일 전자상거래의고객니즈관리에 관한 문헌고찰을 통하여 설문지를 개발하였으며 온라인 설문조사를 실시하여 새로운 변수개발에 적합한 PLS 분석법을 적용하여 수집된 데이터를 제안된 예측연구모델에 적합한가의 여부를 검증하였다. 모바일 전자상거래만을 사용하여 구매활동을 하는 99명의 유효한 중국인 대상 표본을 추출하였으며, 고객니즈관리 측정모형 분석을 위한 분석 도구로 20문항으로 구성된 설문지를 제작하여 사용하였으며, 수집된자료는 SmartPLS 3.0을 이용하여 분석하였다. 분석결과 e-고객니즈관리를 구성하는 고객니즈이해, 고객니즈파악, 고객응대, 고객소통의 1차순 구성개념들은 반영지표(mode A)로 구성이 되었으며 구성개념들 모두 e-고객니즈관리를 예측하는 조형지표(mode B)로 구성이 되는 반영-조형 모형Ⅱ로 구성이 됨을 CTA-PLS 접근법을통하여 검증하였다. 중국 전자상거래의 구매행위에 영향을 미치는 e-고객마케팅 관점에서 데이터 기반의실증자료를 제시함으로써 e-고객니즈관리에 영향관계를 파악할 수 있는 구조모델구축에 기초적인 참고자료를 제시하고자 하였다.

      • KCI등재

        지각된 공유경제 서비스 품질 리스크의 고차순 구성개념에 관한 반복지표접근법의 식별과 추정에 관한 연구

        이건형 국제e-비즈니스학회 2019 e-비즈니스 연구 Vol.20 No.7

        This study aims to specify and estimate the measurement model and the structural model in terms of higher-order constructs by exploring the factors of perceptual risk by consumers using sharing economic products or services. Questionnaires for the survey were developed through literature review on sharing economic service, perceived risk, and hierarchical component model. By conducting an online survey, the significance and relevance of the proposed model were verified by applying the PLS analysis method. A valid sample of 169 experienced users who have used sharing economic services was extracted using convenience sampling, and a questionnaire consisting of 26 items was produced and used to analyze the perceptual service quality risk measurement model(PSQR), which is a higher-order construct, and the structural model affecting discomfort and unacceptable intention. The collected data were analyzed by repeated indicator approach and extended repeated indicator approach using SmartPLS 3.0. As a result of the analysis, reliability and validity were confirmed to be moderate in the specification and estimation of the lower-order constructs of time risk, physical risk, psychological risk, and functional risk constituting the perceptual service quality risk, and the validity of the formative constructs that predict the perceptual service quality risk, which is a higher-order construct, was confirmed to be moderate. In the structural model analysis, the reflective-formative model (type II) was confirmed to be moderate for the verification of significance and relevance. This study aims to suggest basic reference data for specifying measurement model and building a structural model that can grasp the influential relationship by presenting data-based empirical data from the perspective of service quality risk affecting sharing economic service providers and consumer actors. 본 연구는 공유경제 제품 또는 서비스를 이용하는 소비자들이 지각하는 리스크에 대한 요인들을 탐색하여측정 모형과 구조모형을 고차순 구성개념의 측면에서 식별하고 추정하고자 하였다. 공유경제 서비스, 지각된리스크, 위계적성분모형에 관한 문헌고찰을 통하여 설문을 위한 문항들을 개발하였으며 온라인 설문조사를실시하여 PLS 분석법을 적용하여 수집된 데이터를 제안모형의 유의성과 적합도 여부를 검증하였다. 공유경제서비스를 사용해본 경험자 169명의 유효한 대상 표본을 편의표집으로 추출하였으며, 고차순 구성개념인지각된 서비스품질리스크 측정모형과 불쾌감과 불수용의도에 영향을 미치는 구조모형 분석을 위하여 26문항으로 구성된 설문지를 제작하여 사용하였으며, 수집된 자료는 SmartPLS 3.0을 이용하여 반복지표접근법과확장된 반복지표접근법으로 분석하였다. 분석결과 지각된 서비스품질리스크를 구성하는 시간적 리스크, 신체적 리스크, 심리적 리스크, 성능적 리스크의 반영적 저차순 구성개념들의 식별과 추정에서 신뢰도와 타당도가적합한 수준으로 확인되었으며 고차순 구성개념인 지각된 서비스품질리스크를 예측하는 조형적 구성개념의타당도 적합하게 확인되었다. 구조모형 분석에서는 반영-조형 모형(유형 Ⅱ)로 유의성과 적합도 검증에서적합하게 확인되었다. 공유경제 서비스 제공자와 소비 행위자에게 영향을 미치는 서비스품질 리스크 관점에서 데이터 기반의 실증자료를 제시함으로써 영향관계를 파악할 수 있는 측정모형 식별과 구조모형 구축에기초적인 참고자료를 제시하고자 하였다.

      • KCI등재후보

        만경강 하류에서의 종속영양 세균의 계절적 분포와 항생제 내성세균의 특성

        이건형,이규춘 한국육수학회 1993 생태와 환경 Vol.26 No.2

        To determine the characteristics and distribution of antibiotic resistant bacterial populations in the lower Mankyoung River, a total of 106 bacterial strains isolated were examined for susceptibility to 8 antibacterial agents. Isolates identified as to genus by API kits were revealed as Pseudomonas spp.(26 strains), Enterobacter spp.(25), Acinetobacter spp.(17), Escherichia spp.(16), Shigella spp.(6), Moraxella spp.(5), Klebsiella spp.(4), Citrobacter spp.(3), Aeromonas spp.(1), Serratia spp. and 2 unidentified strains. Isolates by the high concentration disc diffusion technique showed higher incidence of resistance to ampicillin(AM), cephalothin(CF), and tetracycline(Te), but most of them showed sensitivity to gentamicin(GM) and amikacin(AN). A total of 20 different patterns of multiple antibiotic resistance were observed. An increase of multiple antibiotic-resistant bacteria was partly was antibiotic resistance transfer to indigenous bacteria must be considered on risk assessment of the release of antibiotic resistant microorganisms in the environment.

      • KCI등재후보

        만경강하류 생태계에서의 종속영양 세균의 계절적 분포와 이화학적 특성

        이건형,이영옥,이규춘 한국육수학회 1991 생태와 환경 Vol.24 No.1

        This study was carried out bimonthly from October, 1989 to August, 1990. As a part of preliminary ecological survey to elucidate the struture and function of lower Mankyong River ecosystem. Water temperature, pH, salinity and inorganic nurients (ammonia-N, nitrite-N, nitrate-N and phosphate-P) were measured. Annual variation of total heterotrophic bacteria, physiologically characteristic bacteria and fecal coliform bacteria were also measured for microbiological analysis. When we considered the parameters in all its aspects, water quality near Mokchon Bridge (St. 4) was remarkably polluted as compared with other stations. Total heterotrophic bacterial population during investigation periods was ranged from 5.0 x 10³ c.f.u. ml to 1.3 x 10^6 c.f.u. ml. Seasonal mean values of proteolytic bacterial populations showed higher densities than those of lipolytic and amylolytic bacteria at all stations. Fecal pollution near St. 4 was more serious than other stations. Among 93 isolates, 55.9% were rods and 64.5% were Gram negative. According to morphological, physiological, nutritional and biochemical tests, isolated bacteria adapted not only broad range of water temperature, pH, and salinity but also various kinds of nutrients.

      • SCOPUSKCI등재

        하구퇴적토 환경에서 E.coli의 부착에 영향을 주는 물리화학적 요인

        이건형 한국미생물학회 1988 미생물학회지 Vol.26 No.3

        The higher bacterial numbers on clay than on sand were caused by different environmental factors. Such factors affecting the adsorption of E. coli ATCC 11775 in the sediment as follows; optimal pH range for the adsorption of E. coli ATCC 11775 was pH 7.5-pH 9.5. E. coli ATCC 11775 were shown maxima in the salinity of 18.$75%_{o}$ on sand type sediment and $12.5%_{o}$ on clay type sediment. Bacteria attached better to clay typed sediment than to sand typed sediment when organic substance was eliminated. Beef extract of 0.5%-1% concentration was found to promote the attachment of E. coli ATCC 11775 effectively. Peptone of 0.5% was enganced the attachment on the clay, and peptone of 1.3%-5%, on the sand. E. coli ATCC 11775 was found to adsorb onto benthonite with the highest efficiency and on celite with the lowest efficiency. Efficiency of adsorption by inorganic ions was shown due to higher values of ion. Adsorption was achieved in the order of $Al^{3+}, Ca^{2+}, Na^{+}$.

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