백서와 기니픽의 대뇌피질에서 Opioid Kappa 수용체의 특성에 관한 연구

김기원(Kee-Won Kim),노혜원(Hye-Won Rho),김형일(Hyoung-Il Kim),은재순(Jae-Soon Eun),소수미(Soo-Mi Soh),조규박(Kyu-Park Cho) 대한약리학회 1994 대한약리학잡지 Vol.30 No.2

In this study, we tested the influences of several κ opioid ligands on the [³H]diprenorphine binding in rat and guinea pig cortex membrane preparations. Using paradigm to block μ and δ opioid receptors with DAMGO(1μM) and DPDPE(1μM), [³H]diprenorphine labeled κ sites. Competition analysis in both rat and guinea pig cortex has shown a single population of [³H]diprenorphine binding site with different Kd values, respectively. There is a significant difference in Ki values of (-) WIN44441 and (+)WIN44441 in both rat and guinea pig cortex. Bremazocine, (-)ethylketocyclazocine, (-)cyclazocine, nor-binaltorphimine effectively inhibited the [³H]diprenorphine binding with different Ki values in rat and guinea pig cortex. U-69,593, U-50,488H and dynorphine-A (1-8) did not inhibit the [³H]diprenorphine binding in rat but in guinea pig cortex. Nor-binaltorphimine was a ligand discriminate the κ₁, and κ₂ receptor most effectively. We, also, examined the influence of Na ion and GTPγS, a nonhydrolyzable guanine nucleotide analog, on the inhibition of [³H]diprenorphine binding by diprenorphine, (-)ethyl-ketocyclazocine, U-69,593 and bremazocine. By the replacement of NaCl with N-methy-D-glucamine or addition of GTPγS, Ki values of diprenorpnine were not changed and that of ethylketocyclazocine were changed significantly in both rat and guinea pig cortex. The Ki value of bremazocine was decreased by removal of Na ion, and increased by GTPγS, however, was not changed by any one of either. These results suggest that there are 2 kinds of subtypes of κ opioid receptor, κ₁, and κ₂, showing different Ki values for various κ opioid ligands, also, bremazocine possess the antagonistic property at κ₂ site which is dominant subtype of K receptor in rat cortex.

알록산 투여로 유발된 실험적 당뇨병에 대한 사인추출물의 치료 효과

노혜원,이지나,구본선,조정임,박진우,김형로 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.26 No.2

연구배경:사인추출물이 알록산에 의한 당뇨 유발을 억제하는 효과가 있음을 이미 확인하였던 바 사인 추출물이 췌장 베타세포가 손상되어 유발된 당뇨병의 치료에 사용할 수 있는지의 가능성을 알아보았다. 방법:실험적 당뇨는 알록산(60㎎/㎏)을 생쥐 꼬리 정맥을 통해 주사하여 유발시켰다. 당뇨에 미치는 사인추출물의 영향을 관찰하기 위하여 사인추출물의 영향을 관찰하기 위하여 사인추출물(2.5㎎/생쥐)을 하루에 두 번 생쥐 복강 내로 투여하였다. 당뇨에 미치는 사인추출물의 영향을 관찰하기 위하여 생쥐에 사인추출물을 처리한 다음 혈당 및 혈청 인슐린 농도 변화 및 조직학적인 소견으로 확인하였다. 결과:생쥐에 알록산을 투여하면 췌장 베타세포가 파괴되어 고혈당 및 hypoinsulinemia가 유발되었다. 사인추출물을 알록산 투여 전 2일 동안 전처리하면 알록산에 의한 고혈당이 완전히 억제되었다. 뿐만 아니라 알록산에 의해 유발된 췌장 베타세포의 손상은 사인추출물의 투여로 현저히 개선되어 혈당 및 혈청 인슐린 농도가 거의 정상 수준으로 회복되었다. 알록산을 투여한 생쥐의 췌장 소도는 정상군에 비해 수가 현저히 감소되었을 뿐 아니라 남아있는 소도의 크기도 현저히 줄어들어 있었으나, 사인추출물을 처리하면 모두 회복되어 정상군과 유사한 양상을 보였다. 결론:사인추출물은 알록산에 의해 유발되는 당뇨병을 억제하는 방어 작용 뿐만 아니라, 알록산 투여로 이미 손상된 췌장 베타세포를 개선시킬 수 있는 치료 효과작용도 가지고 있음을 확인하였다. 이를 토대로 사인의 당뇨 개선 기전을 규명하면 당뇨병 치료제로서 이용할 수 있으리라 사료된다. Background : During the screening of natural products for potential antidiabetogenic components, a strong protective effect of Amomum xanthoides extract on alloxan-induced β-cell damage and in a mice diabetic model. In this study, the therapeutic effect of Amomum xanthoides extract was investigated after induction of diabetes by alloxan. Methods : Experimental diabetes was induced by the injection of alloxan(60mg/kg) to the mouse via the tail vein. To examine the effect the of Amomum xanthoides extract on diabetes, Amomum xanthoides extract (2.5mg/mouse) was admini-strated intraperitoneally. The effect of the Amomum xanthoides extract on alloxan-induced diabetes was observed by measuring the blood glucose and serum insulin level, and a histological examination. Results : Alloxan caused hyperglycemia and hypoinsulinemia by a selectively destroying pancreatic β-cell. Pretreating the with an Amoumum xanthoides extract completely protected them from the hyperglycemia induced by alloxan. In addition, the Amomum xanthoides extract administe 3 days after the of alloxan injection significantly abolished the hyperglycemia and hypoinsulinemia induced by alloxan. The alloxan-treated mice showed a marked change of in the pancreatic islets: the number of islets was reduced and the size of the remaining islets also decreased. However these effects of alloxan were significantly recovered by a later administration of the Amomum xanthoides extract. Conclusion : The amomum xanthoides extract contains potentially effective components, which both protect and treat alloxan-induced diabetes. The identification and action mechanism of the effective components of the Amomum xanthoides extract requires further investigation and it is suggested that the Amomum xanthoides extract be used as a therapeutic drug for diabetes(J Kor Diabetes 26:126~133, 2002).

Protective Effect of Acanthopanax senticosus Extract on Alloxan - induced β - cell Damage

Hye-Won Rho,Ji-Hyun Lee,Jong-Suk Kim,Hyung-Rho Kim,Byung-Hyun Park,Jin-Woo Park 한국식품영양과학회 2005 Preventive Nutrition and Food Science Vol.10 No.1

The protective effect of Acanthopanax senticosus (AS) extract on alloxan-induced pancreatic β-cell damage was investigated in HIT T-15 cells, a Syrian hamster pancreatic β-cell line. Alloxan caused the pancreatic β-cell damage through the generation of reactive oxygen free radicals, increased DNA fragmentation, and decreased cellular NAD^+ levels. The β-cell damage was significantly prevented by the pretreatment with water soluble extract of AS roots. These results suggest that the protective effect of AS extract, on alloxaninduced β-cell damage, is primarily due to the inhibition of the generation of reactive oxygen free radical species (ROS) by alloxan.

Systematic Relationships of the Urochordates Based on Partial 18S rDNA Sequences

Won, Hye-Won,Rho, Boon-Jo,Song, Jun-Im The Korean Society for Integrative Biology 1999 Korean journal of biological sciences Vol.3 No.4

Urochordates, the most primitive group in phylum Chordata, are mostly sessile as adults although some are free living. Presently, the ancestral stock of urochordates as weir as chordates has been the focus of interest and two conflicting hypotheses have been presented. A free swimming ancestor is one and a sessile, filter feeding ancestor is the other. To clarify the phylogenetic relationships within the urochordates, 22 urochordates and five others as outgroups were used. And we applied neighbor joining, maximum likelihood, and maximum parsimony methods to partial 18S rDNA sequences. The inferred phylogeny in all analyses indicates that order Aplousobranchia of class Ascidiacea appears to be the most ancestral group among urochordates. But it is not clear for the low bootstrap value. The remaining two orders of ascidians, Phlebobranchia and Stolidobranchia, form monophyletic groups respectively, which are well supported by high bootstrap values. These two orders are closer to classes of Thaliacea and Appendicularia than to the Aplousobranchia. While class Appendicularia is strongly supported by the monophyletic group, the phylogenetic position of class Thaliacea is unclear in this study.

Protective Effect of Acanthopanax senticosus Extract on Alloxan-induced β-cell Damage

Rho, Hye-Won,Lee, Ji-Hyun,Kim, Jong-Suk,Kim, Hyung-Rho,Park, Byung-Hyun,Park, Jin-Woo The Korean Society of Food Science and Nutrition 2005 Preventive Nutrition and Food Science Vol.10 No.1

The protective effect of Acanthopanax senticosus (AS) extract on alloxan-induced pancreatic β-cell damage was investigated in HIT T-15 cells, a Syrian hamster pancreatic β-cell line. Alloxan caused the pancreatic β-cell damage through the generation of reactive oxygen free radicals, increased DNA fragmentation, and decreased cellular NAD/sup +/ levels. The β-cell damage was significantly prevented by the pretreatment with water soluble extract of AS roots. These results suggest that the protective effect of AS extract, on alloxan-induced β-cell damage, is primarily due to the inhibition of the generation of reactive oxygen free radical species (ROS) by alloxan.

원저 : 마우스 소장조직 (小腸組織)의 Ornithine Aminotransferase 에 관한 연구

노혜원(Hye Won Rho),최경수(Kyung Soo Choi),임방은(Bang Eun Lim),김종석(Jong Suk Kim),박병현(Byung Hyun Park),김형로(Hyung Rho Kim) 전북대학교 의과학연구소 1991 全北醫大論文集 Vol.15 No.4

[P5-25] The Effect of Fermentation on Ascorbic Acid or Citric Acid Treated Ginseng Powder

Hye Ri Shin,Young-Chul Lee,Kyungtack Kim,Jeonghae Rho,Hee-Do Hong,Young-Chan Kim,Sang Yoon Choi,Chang-Won Cho,Young Kyoung Rhee 한국식품영양과학회 2009 한국식품영양과학회 학술대회발표집 Vol.2009 No.11

마우스 小腸組織의 Ornithine Aminotransferase에 關한 硏究

노혜원,최경수,임방은,김종석,박병현,김형로 의과학연구소 1991 全北醫大論文集 Vol.15 No.4

Ornithine aminotransferase catalyzes the transfer of δ-amino group L-ormithine to α-ketoglutarate to yield glutamic- γ - sernialdehyde and glutamate. In present study, ornithine aminotransferase from mouse small intestine was puriried and its biochemical properties were studied. 1.Ornithine aminotransferase activity ws higher in mouse small intestine than in liver and kidnery. The enzyme ws found to be mostly located in cytosolic fraction of small intestine, but present in mitochondrial fraction of liver and kidney. 2.The cytosolic enzyme was very unstable, but the stabiling was enhanced by the presence of EDTA and glycerol. 3.The cytosolic ornithine aminotransferase was purified to homogeniety from mouse small intestine by heat treatment, calcium-phosphate gel adsorption, DEAE= cellulose chromatography, Sephadex G-100 gel filtration and Protein Analysis I-125 gel filtration. The specific a activity of 240-fold purified enzyme was 806 unit/mg protein. 4.The purified enzyme is composed of a single polypeptide chain with molecular weight of 46,000-47,000 daltons and its optimum pH was 8.5. The isoelectric point of the enzyme was found to be pH 5.3, suggesting that the content of acidic amoino acids is relatively high in the enzyme protein.

Virbrio Vulnificus가 분비하는 Cytolysin의 성상

노혜원,김종석,양병철,최원용,김형로 의과학연구소 1989 全北醫大論文集 Vol.13 No.1

The cytolysin produced by Vibrio vulnificus was isolated and its properties were studied. 1. The cytolytic activity in the supernatant of culture media reached to the maximum about 4-5 hours of culture and decreased rapidly thereafter. Meanwhile, the protease activity increased with the time until 8 hours of culture and maintained during culture period of 12 hours. 2. The cytolysin was lethal for mouse and its LD^50 for mouse ws estimated as 3.7 hemolytic units. 3. The cytolysin was very active against sheep and mouse erythrocytes, while it was less active against rabbit and human erythrocytes. 4. The cytolytic activity was maximum in the presence of 0.5mg/ml BSA in assay mixture. (Key words : Vibrio vulnificus, cytolysin, protease)

A Systematic Study on Dendrochirotida (Echinodermata: Holothuroidea) in Korea

Rho, Boon Jo(노분조),Won, Jung Hye(원정혜) 한국동물분류학회 1993 Animal Systematics, Evolution and Diversity Vol.9 No.2