방풍의 항염 효과 기전

노성일 ( Sung Il Noh ),김상돈 ( Sang Don Kim ),박성철 ( Sung Cheul Park ),서병윤 ( Byung Yun Seo ),염승룡 ( Seung Ryong Yeom ),권영달 ( Young Dal Kwon ),신병철 ( Byung Cheul Shin ),송용선 ( Yung Sun Song ) 한방재활의학과학회 2005 한방재활의학과학회지 Vol.15 No.2

목적 : 방풍은 임상적으로 관절염을 포함한 다양한 염증성 질환 치료제로 사용되어 왔다. 본 연구에서는 인간 비만세포를 이용하여 세포 독성에 영향을 주지 않는 농도에서 방풍의 항염 효과 및 그 기전을 검토했다. 방법 : 인간의 HMC-1세포를 IMDM에서 페니실린, 스트렙토마이신, 모노티오글리세린를 첨가하여 배양하고 방풍추출액을 투여하였다. 그 다음 MTT, ELISA, RT-PCR, 세포내 칼슘측정, 핵단백분석을 이용하여 TNF-α, IL-6, IL-8 각각의 형성과 mRNA발현, 세포내 칼슘 수준, NF-κB 발현에 대한 방풍추출액의 반응을 측정하고 통계처리 하였다. 결과 : 방풍은 PMA와 calcium ionophore A23187로 활성화된 비만세포에서 세포내 칼슘 수준과 NF-κB, TNF-α와 IL-6의 발현을 억제 시켰고 RT-PCR을 이용한 mRNA 발현에서 TNF-α와 IL-6의 발현을 억제하였다. 결론 : 방풍은 비만세포내 칼슘 수준 및 NF-κB의 활성을 억제하고 염증성 세포 활성 물질인 TNF-α와 IL-6의 분비도 억제하여 항염 효과를 나타냄을 암시하고 있다.

GATA-3 is a Key Factor for Th1/Th2 Balance Regulation by Myristicin in a Murine Model of Asthma

이규,이창민,정인덕,정영일,천성학,박희주,최일환,안순철,신용규,이상율,염석란,김종석,박영민,Lee, Kyu,Lee, Chang-Min,Jung, In-Duk,Jeong, Young-Il,Chun, Sung-Hak,Park, Hee-Ju,Choi, Il-Whan,Ahn, Soon-Cheol,Shin, Yong-Kyoo,Lee, Sang-Yull,Yeom, S Korean Society of Life Science 2007 생명과학회지 Vol.17 No.8

Myristicin은 육두구에서 발견되는 고농축 정유 중 하나인 물질이다. 하지만 Th1/Th2 면역반응에서 육두구의 항알레르기 효과는 아직 밝혀지지 않았다. 최근에 Th1/Th2 전사인자로서 T-bet, GATA-3가 밝혀졌는데 이번 실험에서 myristicin이 ovalbumin(OVA)으로 유도한 천식(asthma) 생쥐모델에서 Th1,Th2 싸이토카인과 유전자 발현을 조절할 수 있는가에 대하여 알아보았다. 또한 기관지 폐포 세척액을 회수하여 백혈구의 수적 변화, 제2형 협조T세포(Th2 cell)가 생산하는 IL-4, IL-5의 생산에 미치는 영향과 폐조직에서 matrix metalloproteinase (MMP)-9 활성을 측정하였다. 그 결과 기관지 폐포 세척액에서 OVA로 감작하여 천식을 유도한 실험군에서는 호산구의 현저한 증가, Th2 형 싸이토카인(IL-4, IL-5)의 증가가 관찰되었다. 그러나 myristicin을 투여한 그룹에서는 OVA의 감작에 의하여 증가한 각종 염증성 지표들이 감소하거나 정상화 되었다. 또한 OVA에 의하여 증가된 기도저항성이 myristicin 투여에 의하여 감소하였으며 폐조직의 염증성 소견도 뚜렷하게 감소되었다. 이와 같은 연구 결과는 myristicin이 천식의 치료에 유용하게 쓰일 수 있음을 시사해준다. Myristicin, l-allyl-3,4-methylenedioxy-5-methoxybenzene, was one of the major essential oils of nutmeg. However, its anti-allergic effect in the Th1/Th2 immune response was poorly understood. Recently, it was shown that T-bet and GATA-3 was master Th1 and Th2 regulatory transcription factors. In this study, we have attempted to determine whether myristicin regulates Th1/Th2 cytokine production, T-bet and GATA-3 gene expression in ovalbumin (OVA)-induced asthma model mice. Myristicin reduced levels of IL-4, Th2 cytokine production in OVA-sensitized and challenged mice. In the other side, it increased $IFN-{\gamma}$, Th1 cytokine production in myristicin administrated mice. We also examined to ascertain whether myristicin could influence eosinophil peroxidase (EPO) activity. After being sensitized and challenged with ovalbumin (OVA) showed typical asthmatic reactions. These reactions included an increase in the number of eosinophils in bronchoalveolar lavage fluid, an increase in inflammatory cell infiltration into the lung tissue around blood vessels and airways, and the development of airway hyper-responsiveness (AHR). The administration of myristicin before the last airway OVA challenge resulted in a significant inhibition of all asthmatic reactions. Accordingly, these findings provide new insight into the immunopharmacological role of myristicin in terms of its effects in a murine model of asthma.

자동차 조립작업자의 사회ㆍ심리적 스트레스와 근골격계 증상과의 연관성

김일룡,김재영,박종태,최재욱,김해준,염용태 대한산업의학회 2001 대한직업환경의학회지 Vol.13 No.3

목적 : 이 연구는 자동차 조립라인 근로자들의 사회·심리적 스트레스와 작업관련 근골격계 자각증상간의 연관성을 확인하는 데에 있다. 방법 : 경인지역 소재 자동차 회사 두곳의 조립라인에 근무하는 근로자 636명을 대상으로 하여, 근골격계 자각증상에 대해서는 목과 어깨, 팔과 팔꿈치,. 손목과 손, 허리부위에서의 통증, 쑤심, 뻣뻣함, 화끈거림, 무감각 또는 저림 등을 묻는 표준화된 설문지로 질문하였고, 스트레스 정도는 4개 분야, 45개 항목으로 이루어진 Psychosocial Well-being Index 설문지를 배포후 3점 척도를 적용하였다. 이들간에는 카이자승법, ANOVA rm리고 로짓회귀분석을 사용하여 통계적으로 유의한 연관성을 알고자 하였다. 결과 : 1. 수면시간을 제외하고 연령, 근속기간, 결혼여부, 학력수준, 흡연력, 음주력에 따른 상지근골격계 증상의 빈도 차이는 없었다. 2. 30세 미만의 작업자군에서 사회 심리적 스트레스 점수(p-value: 0.0461)와 스트레스 Factor 3 (p-value: 0.0368), Factor 4 (p-value: 0.0053)의 점수가 높았다. 3. 11∼15년의 근속기간을 가진 작업자의 경우 Factor 1을 제외한 PWI(p-value: 0.0001), Factor 2(p-value:0.0001), Factor 3(p-value: 0.0004), Factor 4(p-value: 0.0001)에서 기타의 근속기간을 갖는 동료에 비해 높은 스트레스 점수를 보였다. 4. 미혼자는 Factor 4에서 기혼자보다 높은 스트레스 점수를 보였다(p-value: 0.0068). 5. 수면시간이 6시간 이하인 작업자군은 6시간을 초과하는 작업자보다 PWI(p-value: 0.0087), Factor 2(p-value: 0.0194), Factor 3(p-value: 0.0048), Factor 4(p-value: 0.0314) 등에서 높은 스트레스 점수를 보였다. 6. 스트레스 점수는 정규분포를 이루었으며 정상군과 스트레스위험군간에 증상의 빈도 차이는 없었다. 7. 근골격계 증상이 있는 작업자군은 무증상군과 비교하여 기타 스트레스 점수는 두 군간에 유의한 차이가 없었으나, Factor 2에서 통계적으로 유의하게 높은 스트레스 점수를 보였고(p-value: 0.028), 단변량(OR: 1.02) 및 다변량(OR: 1.04) 로짓 회귀분석에서도 Factor 2가 양의 회귀계수(0.0226; 0.0352)를 보여 증상에 영향을 주는 것으로 나타났다. 결론 : 근골격계 자각 증상이 있는 조립작업자군은 증상이 없는 군에 비해 우울증 항목에서 높은 스트레스 점수를 보였으나 기타 사회적 역할 수행 및 자기신뢰도, 수면장애 및 불안, 일반건강 및 생명력등의 사회심리적 스트레스 항목에서는 두 군간에 유의한 차이를 보이지 않았다. 로짓회귀분석예서도 우울증이 근골격계 증상에 영향을 주는 것으로 나타나서 비록 제한적이지만 우울증에 해당하는 항목인 Factor 2와 증상간의 연관성을 일부 확인할 수 있었다. 그러나 근골격계 증상발현에는 스트레스 이외에도 인간공학적 혹은 알려지지 않은 요인들 중요하게 작용하므로 이들을 통제한 후의 추가적인 조사를 해야 할 것으로 생각된다. Objectives : To determine the relationship between stress Factors and work-related muscul-loskeletal symptoms of assembly line workers in the automobile industry. Methods : A cross-sectional study was conducted in two Factories of automobile manufacturing companies where inappropriate posture and repetitive motions were required. The total number of subjects was 636, and consisted entirely of men. The Age, length of work duration, marital status, education level, smoking status, drinking status, sleeping time and stress scores were investigated according to subgroups of general characteristics. We divided the subjects into a Reference group and a Stress risk group to compare the frequency of the variables between them. The stress scores of the Positive symptom group were compared with the Symptom free group by the t-test. To measure the stress level, the PWI(Psychosocial Well being Index) which consists of 4 Factors, totaling 45 items, was used. A standardized self-reported questionnaire was used to assess the symptoms in workers. The criteria for positive symptoms were based on Operational Definition of Work-related Musculoskeletal Disorders of the NIOSH. Results : 1) The subgroups of Age, Work duration, Marital status, Education level, Smoking status, Drinking status and Sleeping time revealed no differences in frequencies in musculoskeletal symptoms. 2) Higher PWI(p<0.05), Factor 3(p>0.05) and Factor 4(p<0.01) scores were observed in younger workers compared with older workers. 3) The workers who had work durations betwen 11-15 years showed higher PWI(p<0.01), Factor 2(p<0.01), Factor 3(p<0.01), and Factor 4(p<0.01), compared with other work duration subgroups. 4) Singles had higher Factor 4 scores(p<0.01). 5) The sleeping time of less than 6 hours a day expressed a higher PWI(p<0.01), Factor 2(p<0.05), and Factor 3(p<0.01), Factor 4(p<0.05). 6) There was no difference in the prevalence of musculoskeletal symptoms between the Reference group and Stress risk group. 7) The subjects who had Positive musculoskeletal symptoms showed a significant difference in Factor 2 scores compared with the Symptom free groups. 8) According to a univariate logistic regression analysis, Factor 2(OR: 1.02, 95% CI:1.00-1.04, p-value: 0.0291) expressed significant but mild effects on the musculoskeletal symptoms and a multivariate logistic regression showed a statistically significant effect on musculoskeletal symptoms(OR:1.04, 95% CI: 1.01-1.07, p-value: 0.0170). Conclusion : Psychosocial stress scores were not higher in symptomatic subjects compared with those who had no symptoms. Sleeping longer showed a protective effect on symptoms but this was not statistically significant. There was no relationship between the stress scores and musculoskeletal symptoms in social performance and self-confidence, general well-being and vitality. Factor 2(Depression) was statistically significant though its effect was mild. Limited to this study, We could find partial relationship between psychosocial stress(Depression) and musculoskeletal symptoms. So it could therefore be suspected that ergonomic or other unknown factors may be more significant causes of musculoskeletal symptoms but we did not investigate these.

21세기 쾌적한 삶의 공간확보를 위한 도시정책의 방향 : 마을 만들기 운동을 중심으로

이용연,염일열 서강정보대학 2001 論文集 Vol.20 No.-

The village-making movement, which started in 1962 from the citizen movement for redevelopment of eito district, in nagoya city, Japan, led to citizen's participation in the city planning. This movement has developed from the completion and reform activity tho the principal strategy for citizen's health, welfare, education, the identity establishment and the activation for the region. Korea has developed Jumin-Undong(the resident or the citizen movement) since the second age of the local self-governing, which is similar to the village-making movement in Japan. Jumin-undong ties up their heart and mentality beween residents and residents and develops the movement for creating the local community. This study seeks to alternatives for establishing the space of pleasant life and activating the village-making movement in the twenty-first century.

불소의 농도와 Seed Material이 Crystal Growth에 미치는 영향

오승연,정일영,금기연,이찬영 大韓齒科保存學會 1997 Restorative Dentistry & Endodontics Vol.22 No.2

The present study was undertaken to investigate the crystal growth onto human enamel mineral and synthetic hydroxyapatite(HA) seeds in media resembling the enamel fluid composition. Effects of fluoride at high concentrations on the precipitation were also examined in a bench-top crystal growth model adopting a miniaturized reaction column. The Ca, P and F concentrations and pH values of the inlet and outlet solutions were determined. The recovered solid samples were weighed to assess the amount of minerals precipitated during the experimental period, and finally viewed under a scanning electron microscope. Remarkable findings were that 1) both biological and synthetic seeds with the same total surface areas yielded similar amounts of crystal growth, 2) the amount of crystal growth was accelerated in a manner depending of fluoride concentrations in the media, 3) SEM observations disclosed that without the addition of fluoride, precipitation of thin, plate-like OCP crystals became prominent, but by increasing the fluoride concentration(beyond 1ppm F), rod-like crystals having a pointed edge were most frequently observed, without and evidence for precipitation of the plate-like crystals. Furthermore, the dimension of rod-like crystals was increased in proportion of fluoride concentrations, 4) there was no difference in the morphological feature of precipitated mineral phase upon seeding between human enamel seed and synthetic HA seed. The overall results support the view that the seeded crystal growth model is of value to gain insight into the mechanism of enamel crystal growth under fluoride regimens.

New representation of greninger curves and simulation of laue photographs

Yeom, Yuo YoungYoon, Il HoPark, Young-Han 檀國大學校 基礎科學硏究所 1995 論文集 Vol.6 No.-

Role of E2F1 in Endoplasmic Reticulum Stress Signaling

Young Il Yeom,Kyung Mi Park,Dong Joon Kim,Sang Gi Paik,Soo Jung Kim 한국분자세포생물학회 2006 Molecules and cells Vol.21 No.3

Preparation of Glutamic Acid-Leucine Copolymer Containing Indomethacin for Controlled Delivery

Yeom.Young IL 江原大學 生命科學硏究所 1986 生命科學硏究所報 Vol.4 No.1

Preparation of Glutamic Acid-Leucine Copolymer Containing Indomethacin for Controlled Delivery

Yeom, Young-Il,Kim, Hyun-Pyo,Kim, Hack-Joo,Byun, Si-Myung,Kim, Nam-Deuk Korean Chemical Society 1986 Bulletin of the Korean Chemical Society Vol.7 No.3

A series of copolypeptides of glutamic acid and leucine have been synthesized by N-carboxy-${\alpha}$-amino acid anhydride procedure and cast to form injectable microparticulate monolithic devices in which indomethacin was physically dispersed. With these devices, various release properties and possible clinical application were studied. The release rate of the drug had a close relationship with the monomer composition of the copolymer matrix as well as the environmental pH condition. The monolithic device of glutamic acid/leucine = 50/50 was found to be the most promising one as a ploymeric delivery system of indomethacin. The intrinsic viscosity of this copolymer was 4.35 dl/g and the release rate was 18.5${\mu}g/g/day$.

Identification of endothelial cell-specific molecule-1 as a potential serum marker for colorectal cancer

Ji, Na Young,Kim, Young-Ho,Jang, Ye Jin,Kang, Yun Hee,Lee, Chung Il,Kim, Jae Wha,Yeom, Young Il,Chun, Ho Kyung,Choi, Yoon-Ho,Kim, Joo Heon,Kim, Jong Wan,Lee, Hee Gu,Song, Eun Young Wiley (Blackwell Publishing) 2010 CANCER SCIENCE Vol.101 No.10