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Lee, So-Jeong,Lee, So-Gwang,Gwak, Woo-Seok The Korean Society for Integrative Biology 2012 Animal cells and systems Vol.16 No.6
This study uses the mitochondrial DNA control region to identify the genetic diversity and population structure of the marbled soles (Pleuronectes yokohamae) that inhabit Jinhae Bay and Yokji Island in the nearby sea and the adjacent waters of Namhae, Hansan Island, and Jaran Bay. Direct sequencing of the PCR products revealed 379 bp sequences with 83 variable nucleotide sites, defining a total of 91 haplotypes. The haplotype diversity was high, ranging from $0.917{\pm}0.031$ to $0.983{\pm}0.008$, and nucleotide diversity ranged from $0.015{\pm}0.008$ to $0.024{\pm}0.012$. In addition, 48 haplotypes (52.7%) were unique. Pairwise $F_{ST}$ values were very low, with the maximum value occurring between PYH (Hansan Island) and PJI (Jinhae Bay) ($F_{ST}$ = 0.011). Therefore, no significant genetic differentiation was evident between any pair of sampling localities.
Anti-oxidant and anti-inflammatory activities of the various kinds of herbal tea
Lee, Jin Wook,Eo, Hyun Ji,Park, Gwang Hun,Song, Hun Min,Woo, So Hee,Kim, Mi Kyoung,Eom, Jung Hye,Lee, Man Hyo,Lee, Jeong Rak,Koo, Jin Suk,Jeong, Jin Boo The Korea Association of Herbology 2014 大韓本草學會誌 Vol.29 No.2
Objectives : Reactive oxygen species (ROS) are involved in a wide spectrum of diseases including chronic inflammation and cancer. In this study, we investigated the antioxidant activities and anti-inflammatory effects of the extracts from the herbal teas such as Lonicera japonica Thunberg (L. japonica), Chrysanthemum morifolium Ramat (C. morifolium), Mentha arvensis L. (M. arvensis), and P.rhizoma. Methods : Anti-oxidant activity was evaluated using DPPH radical scavenging assay and $Fe^{2+}$ chelating assay. And DNA cleavage assay was performed to evaluate an anti-oxidative effect. Anti-inflammatory effect was performed using NO generation assay and western blot in LPS-stimulated RAW264.7 cell line. Results : L. japonica scavenged DPPH radical by 9.8% at 12.5 ${\mu}g/ml$, 24.8% at 25 ${\mu}g/ml$, 34.3% at 50 ${\mu}g/ml$, 61.1% at 100 ${\mu}g/ml$ and 75.8% at 200 ${\mu}g/ml$, respectively. In addition, C. morifolium and M. arvensis removed DPPH radical by 15.6% and 10.4% at 12.5 ${\mu}g/ml$, 34.8% and 22.8% at 25 ${\mu}g/ml$, 66.9% and 43.3% at 50 ${\mu}g/ml$, 87.4% and 69.1% at 100 ${\mu}g/ml$, and 92.1% and 73.2% at 200 ${\mu}g/ml$, respectively. However, P. rhizoma did not affect on DPPH radical scavenging. The $Fe^{2+}$ chelating activity was highest in L. japonica, but lowest in P. rhizoma among the herbal teas. In addition, the extracts from L. japonica, C. morifolium and M. arvensis inhibited oxidative DNA damage via its anti-oxidant activity. In anti-inflammatory effect, the extracts from C. morifolium inhibited NO production. In addition, it suppressed the $NF-{\kappa}B$ signaling pathway in LPS-stimulated RAW 264.7 cells. Conclusions : Together, this study indicates that L. japonica, M. arvensis and C. morifolium possess the protective effect against the oxidative DNA damage. Furthermore, C. morifolium exerts an anti-inflammatory effect.
( So Young Kim ),( Tae Jin Lee ),( Min Ji Cho ),( Sang-hoon Lee ),( Dong-ho Kim ),( Hyung-mu Park ),( Seung-su Han ),( Gwang-joon Kim ),( Min-yeong Park ),( Eun-ju Lee ) 대한산부인과학회 2016 대한산부인과학회 학술대회 Vol.102 No.-
목적: This study aimed to assess aberrant Dickkopf-3 (DKK3) expression in primary ovarian carcinoma and to determine the role of DKK3 in ovarian carcinogenesis. 방법: DKK3 protein expression was examined by immunohistochemistry using tissue blocks from 82 patients with invasive adenocarcinoma, and 15 normal, 19 benign, and 10 borderline tumors as controls. Clinicopathological parameters of 42 serous adenocarcinomas were reviewed. Survival data were estimated using Kaplan-Meier estimates, and multivariate analysis was performed using the Cox regression method. Anti-proliferative and anti- invasive effects were estimated from colony formation and invasion assays using the SKOV3 ovarian cancer cell line. The expression of β-catenin and matrix metalloproteinases (MMPs) was determined by RT-PCR. 결과: DKK3 was significantly down-regulated in invasive carcinoma compared to normal, benign, and borderline tumors (p<0.001). DKK3 loss occurred in 56.1% invasive carcinoma cases and was significantly associated with chemo-resistance in serous adenocarcinoma (p=0.029). Multivariate analysis showed that women with chemo-sensitivity had a significantly lower risk of recurrence than those with chemo-resistance (p<0.01). Colony formation activity of DKK3-transfected cells was significantly lower than that of control vector-transfected cells (p=0.002). DKK3-overexpressing cells had less invasive capacity and significantly lower MMP-2, MMP-9, and β-catenin than control cells (p<0.01). 결론: Loss of DKK3 expression occurs more often in invasive epithelial ovarian cancer than in normal, benign, and borderline tumors. DKK3 has an anti-proliferative effect and inhibits the invasive capacity of ovarian cancer cells by attenuating MMP-2, MMP-9 and β-catenin indicating that DKK3 is tumor suppressor of ovarian cancer.
Anti-oxidant and anti-inflammatory activities of the various kinds of herbal tea
( Jin Wook Lee ),( Hyun Ji Eo ),( Gwang Hun Park ),( Hun Min Song ),( So Hee Woo ),( Mi Kyoung Kim ),( Jung Hye Eom ),( Man Hyo Lee ),( Jeong Rak Lee ),( Jin Suk Koo ),( Jin Suk Koo ) 대한본초학회 2014 大韓本草學會誌 Vol.29 No.2
Objectives : Reactive oxygen species (ROS) are involved in a wide spectrum of diseases including chronic inflammation and cancer. In this study, we investigated the antioxidant activities and anti-inflammatory effects of the extracts from the herbal teas such as Lonicera japonica Thunberg (L. japonica), Chrysanthemum morifolium Ramat (C. morifolium), Mentha arvensis L. (M. arvensis), and P.rhizoma. Methods : Anti-oxidant activity was evaluated using DPPH radical scavenging assay and Fe2+ chelating assay. And DNA cleavage assay was performed to evaluate an anti-oxidative effect. Anti-inflammatory effect was performed using NO generation assay and western blot in LPS-stimulated RAW264.7 cell line. Results : L. japonica scavenged DPPH radical by 9.8% at 12.5 μg/ml, 24.8% at 25 μg/ml, 34.3% at 50 μg/ml, 61.1% at 100 μg/ml and 75.8% at 200 μg/ml, respectively. In addition, C. morifolium and M. arvensis removed DPPH radical by 15.6% and 10.4% at 12.5 μg/ml, 34.8% and 22.8% at 25 μg/ml, 66.9% and 43.3% at 50 μg/ml, 87.4% and 69.1% at 100 μg/ml, and 92.1% and 73.2% at 200 μg/ml, respectively. However, P. rhizoma did not affect on DPPH radical scavenging. The Fe2+ chelating activity was highest in L. japonica, but lowest in P. rhizoma among the herbal teas. In addition, the extracts from L. japonica, C. morifolium and M. arvensis inhibited oxidative DNA damage via its anti-oxidant activity. In anti-inflammatory effect, the extracts from C. morifolium inhibited NO production. In addition, it suppressed the NF-κB signaling pathway in LPS-stimulated RAW 264.7 cells. Conclusions : Together, this study indicates that L. japonica, M. arvensis and C. morifolium possess the protective effect against the oxidative DNA damage. Furthermore, C. morifolium exerts an anti-inflammatory effect.
Na Gyeong Geum(Na Gyeong Geum),Ju Hyeong Yu(Ju Hyeong Yu),So Jung Park(So Jung Park),Min Yeong Choi(Min Yeong Choi),Jae Won Lee(Jae Won Lee),Gwang Hun Park(Gwang Hun Park),Hae-Yun Kwon(Hae-Yun Kwon),J 한국자원식물학회 2022 한국자원식물학회지 Vol.35 No.6
Under the COVID-19 pandemic, interest in immune enhancement is increasing. Although the immune-enhancing activity of plants of the genus Hibiscus has been reported, there is no study on the immune-enhancing activity of H. syriacus. Thus, in this study, we investigated the immune-enhancing activity of Hibiscus syriacus leaves (HSL) in mouse macrophages, RAW264.7 cells, and immunosuppressed mice. HSL increased the production of immunostimulatory factors such as nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α) and activated the phagocytosis in RAW264.7 cells. The HSL-mediated production of immunostimulatory factors was dependent on toll-like receptor 4 (TLR4), p38, and c-Jun N-terminal kinase (JNK) in RAW264.7 cells. In the immunosuppressed mouse model, HSL increased the spleen index, the levels of the cytokines, and the numbers of lymphocytes, neutrophils, and monocytes. Taken together, HSL may be considered to have immune-enhancing activity and be expected to be used as a potential immune-enhancing agent.