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시안산에 의한 조골세포의 손상에서 아미노산과 알부민의 효과
박경대 ( Kyung Dae Park ),박성배 ( Sung Bae Park ),최혜정 ( Hye Jung Choi ),문교철 ( Kyo Cheol Mun ),김현철 ( Hyun Chul Kim ) 대한신장학회 2007 Kidney Research and Clinical Practice Vol.26 No.4
Purpose : Cyanate, known as one of the uremic toxins and derived spontaneously from urea, has several effects on the biologic substances including erythropoietin, antioxidant and ceruloplasmin. To find out the protective materials from the hazardous effect of cyanate in osteoblast, we added twenty amino acids, albumin globulin and hemoglobin in the culture media containing osteoblastic cells with cyanate. Methods : Osteoblastic ROS 17/2.8 cells, exposed to various concentrations of sodium cyanate, were used to analyze for the cytotoxicity. The cyanate-induced cytotoxicity was assessed by the methylthiazolyldiphenyl -tetrazolium bromide (MTT) assay by measuring the absorbance of the reaction solution at 570 nm. Viability of the treated cells was expressed as A570 of sample/A570 of control. The degree of the carbamylation was measured using trinitrobenzenesulphonic acid. The degree of the carbamylation in amino acid was about 50% in average. Results : The degree of the carbamylation in albumin was increased depending on the incubation time with cyanate and the concentration of the cyanate. The degree of the carbamylation in globulin and hemoglobin was nearly zero. Asp, Glu, Leu, Trp and Tyr among the twenty amino acids revealed the protective effect against the damage induced by cyanate. And only albumin among the three proteins revealed the protective effect. Conclusion : On the basis of these results, Asp, Glu, Leu, Trp, Tyr and albumin are useful tools for the protection against damages by cyanate carbamylation.
최욱(Ook Choi),경대현(Dae-Hyun Kyung),박영성(Yeong-Seong Park) 한국청정기술학회 2014 청정기술 Vol.20 No.3
본 연구에서는 γ-Al₂O₃에 구리를 함침시킨 촉매를 고정층 반응기에 충전시킨 후 휘발성유기물질(VOCs)인 벤젠의 촉매산화 반응특성을 살펴보았다. 실험조건은 반응온도 200~500 ℃, 벤젠의 농도 400~650 ppm, 가스유입량 50~100 cc/min, 공간속도 7,500~22,500 hr<SUP>-1</SUP>의 범위로 적용하였다. BET분석, 주사전자현미경(SEM), 열천칭(TGA) 분석을 통해 제조된 촉매의 물성을 조사하였으며, 벤젠의 촉매산화반응의 전환율에 대하여 고찰하였다. 실험결과, 벤젠의 농도와 공간속도가 낮아질수록 벤젠산화반응의 전환율은 증가함을 알 수 있었다. 벤젠의 촉매산화반응은 1차 균일반응으로 해석될 수 있었으며, 반응의 활성화 에너지(Ea)는 17.2 kcal/mol, 빈도인자(A)는 1.33 × 10<SUP>6</SUP> sec<SUP>-1</SUP>이었다. Catalytic oxidation characteristics of benzene as a VOC was investigated in a fixed bed flow reactor using Cu/γ-Al₂O₃ catalyst. The parametric tests were conducted at the reaction temperature range of 200~500 ℃, benzene concentration of 400~650 ppm, gas flow rate of 50~100 cc/min, and space velocity range of 7,500~22,500 hr<SUP>-1</SUP>. The property analyses by using the BET, SEM, TGA and the conversion of catalytic oxidation of benzene were examined. The experimental results showed that the conversion was increased with decreasing benzene concentration, gas flow rate and space velocity. Benzene oxidation reaction over Cu/γ-Al₂O₃ catalyst could be expressed as the first order homogeneous reaction of which the activation energy was 17.2 kcal/mol and frequency factor was 1.33 × 10<SUP>6</SUP> sec<SUP>-1</SUP>.