The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection

Chun, Yoon-Keun,Ha, Joo-hun,Hong-Jung-Woo,Oh, Soo-Myung,Kim, Sung-Soo 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yoon-Keun Chun¹,Joohun Ha□Hong-Jung Woo□, Soo Myung Oh□,Sung Soo Kim□ ¹Department of Molecular Biology, College of Medicine,²Department of Surgery, college of Medicine,³Department of Internal Medicine, College of Oriental Medicine,and ⁴East-Weat Medical Reserch Institute,Kyung Hee University, Seoul, Korea. The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection. Proceedings of International Symposium on East-West Medicine, Seoul. 212-230, 1999. -Hepatitis B is caused by hepadnavirus. Hepatitis B virus replicates through 3.5kb pregenomic RNA intermediate which is regulated by core promoter. Pathogenesis of hepatitis B virus has been bilieved the result of host immune response. But recently many studies have reported that high level of viral replication caused by mutation in core promoter might result in severs hepatitis. But these studies were performed in vitro, not in vivo. So there is yet debate about which factor, viral of host factor, is more important in pathogenesis of hepatitis B virus. So we measured real viral replication level in 204 chronic hepatitis B patients by quantifying HBV DNA from sera by our novel PCR-based more sensitive method, and compared these results with ALT level measured from same sera, which indicates liver cell damage. Surprisingly there are no significant correlation between HBV DNA quantity and ALT level. Then we cloned core promoter region. In SSCP, we found that many viral mutants coexist in one patient. Base on SSCP result, we chose main viral core promoter type in each patients, which is thought to determine overall viral replication level in this patient. Main type of core promoter region of each 41 patients were directly sequenced. And with these we measured promoter activity by luciferase assay system and compared promoter activity with on another. We found tha there were some differences in promoter activity according to core promoter sequences. And we constructed replication-competent viral constructs with core promoter from 41 patients and Transfected these into HepG2 cell and measured HBV DNA by southern blot. There were also differences in HBV DNA quantity according to core promoter sequences. On these all results we investigated correlation between the effect of HBV core promoter on viral replication in vitro and HBN DNA quantity, ALT level from sera of each patients. We found there is no significant correlation among them. As a result, we concluded that in determining severity chronic hepatitis B patients, host factors of each patient is more important rather than replicative activity of virus itself.

자궁내 발육지연이 있는 출생체중 2,500 g 이상인 환아의 주산기 예후에 관한 연구

송태복,조명숙,하진아,윤대석,김철홍,김윤하,최영륜 全南大醫大 2004 전남의대학술지 Vol.40 No.1

개에서 이중 결찰법을 통한 동맥관 개존증의 완치 예

윤헌영,정순욱,박희명,박철,정만복,김준영,한현정,황민,노병국,박상혁,장하영,박정윤 한국임상수의학회 2004 한국임상수의학회지 Vol.21 No.1

A ten months old, female Yorkshire terrier weighing 2.88 kg referred to veterinary teaching hospital of college of veterinary medicine, Konkuk University because of syncope, cough and dyspnea. First hematological and serum chemical test revealed thrombocytopenia, mild anemia, and increase of concentration of ALP (195 U/L). On 57 days later, second hematological and serum chemical test revealed polycythemia, increase of concentration of ALP (211 U/L), and Tchol (387 mg/dl). Right atrium enlargement, main pulmonary artery bulge and cardiomegaly (VHS = 11.5) were observed in radiographic findings. Ultrasonographic images showed both right and left ventricular dilation and turbulent flow between the descending aorta and the main pulmonary artery in color Doppler imaging. ECG showed left ventricular enlargement, SA block, and electrical alternant. Thoracotomy was performed through left fourth intercostal incision under isoflurane anesthesia. Patent ductus arteriosus was double ligated with 1-0 silk. Cough and dyspnea disappeared on 5 days after operation. Turbulent flow was not found in color doppler imaging of ultrasonography on 10 days after operation. Ten months later after the operation, syncope could not exist any more.

한국대학생의 도덕사태 개념화 및 그 변화과정에 관한 연구(II)

송명자,조용하,설기문,김상윤 東亞大學校 學生生活硏究所 1992 學生硏究 Vol.20 No.-

This study aims to analyze the moral judgmental levels and the conceptions of transgressions of Korean college students in a series of multifacted events and the aspects of change in terms of moral judgmental level and conceptualizastion after with two years' college experience. The study was based on the Kohlberg's cognitive moral development theory and on the domain-distinction model in which children's intuitive abilities are stressed. This study was consisted of two sub-studies done in 1989 and 1991 respectively. The subjects of the 1st year study were 416 college students in four major cities(Seoul, Taegu, Kwangjoo, and Pusan). The subjects of the longitudinal analysis of second year study were 199 college students who could be followed up among the subjects of the 1st year study after two years. The instruments used in this study were Test of Moral Judgmental Development and Test of Conceptualization on Mulifaceted Events developed by the authors on the basis of Kohlberg's and Turiel's theories respectively. The following conclusions were obtained through the basis of the results and discussions of this study. First, Korean college students' false responses to moral issues decrease but the radical and anti-social responses increase after two years, and ther these responses are replaced by the level Ⅲmoral judgmental responses. Second, college students who are on the higher moral judgmental level conceptualize the transgression in social conventional and personal domains less permissible than those on the lower levels do. The students who are on the lower judgmental level reveal the decrese of nonpermissibility in social conventional domain with two years' college experience than those on the higher level do.

알코올의 섭취가 흰쥐의 혈액과 간의 지질대사에 미치는 영향

이명숙,김영연,김효숙,김매하,전진순,서진숙,송선미,양영재,윤원손,이미라,이효영,정승현 誠信女子大學校 生活文化硏究所 2004 生活文化硏究 Vol.18 No.-

To investigate the effects of ethanol on the lipid metabolism in blood and liver, twenty one male Sprague Dawley rats had orally taken the 25% alcohol and 50% alcohol by 5g per Kg of weight during 3 weeks after the adjusting period. For the energy balance, 85% sucrose and 45% sucrose was added in control group and 25% alcohol group each. The weight was reduced by alcohol treatment according to the levels, otherwise the weight of liver and heart was little increased in alcohol groups rather than in control without significance. Moreover, RBC, WBC, hemoglobin and hematocrit were increased by alcohol treatment according to the levels without significance. Otherwise, adjusted bilirubin levels by 100g of weight was significantly increased by alcohol treatment dose dependently. Alcohol induced RBC degradation and increased circulated bilirubin because of liver damage. For blood lipid profiles, blood cholesterol was increased in 50% of alcohol group compared to that of 25% and control groups, otherwise, blood triglycerides (TG) was increased by 30% in 25% alcohol groups rather than in control but not significantly different. The hepatic lipid composition, cholesterol levels in alcohol treatment groups was lower than those in control. The hepatic TG was not influenced by alcohol treatment. The two reasons of decreasing hepatic lipids were suggested by 85% sucrose of control for energy balance more synthesized cholesterol and TG than those in 25 or 50% alcohol groups, and by cholesterol and TG could not be synthesized because of liver damage from alcohol.

열형광선량계에 의한 선량평가 알고리즘 개발

송명재,김희근,하정우,이태영,윤석철 대한방사선 방어학회 1992 방사선방어학회지 Vol.17 No.2

본 연구의 목적은 ANSI N13. 11-1983의 시험기준에서 제시한 개인선량계의 선량평가 시험범주를 모두 만족시킬 수 있는 선량평가 알고리즘을 개발하는데 있다. 알고리즘 개발 대상 개인선량계로는 Teledyne Isotopes사의 PB-3 열형광선량계로 하였다. 개발된 알고리즘에 대한 미국 Atlan-Tech사와의 2차례의 검증결과, 본 알고리즘은 ANSI N-13. 11-1983의 선량평가 범주를 모두 만족시키는 것으로 나타났다. The purpose of this study is to develop an algorithm of TLD dose evaluation to meet all requirements stated in ANSI N13. 11-1983. It made the PB-3 TLD of Teledyne Isotopes an object of the development. Personal dosimetry performance testings of the development algorithm have been performed twice through the Atlan-Tech, INC. in accordance with the criteria of testing described in ANSI N13. 11-1983. As a result, it is assured that the developed algorithm has complied with all requirements stated in ANSI N13-1983.

비글개에서 신원방우황청심원의 급성 및 아급성독성시험

성하정,권오경,방명주,곽형일,이진영,신대희,박대규,정규혁,윤효인,조명행 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

Single and 4 weeks oral administration of New wonbangwoohwangchungsimwon (NSCH) which was used l-muscone as substitutive material of musk, to beagle dogs of both sexes were performed to investigate both acute and subacute toxicity. Beagle dogs (3 males and 3 females) in acute experiments were administered orally with sinlge dose of 2,000 ㎎/㎏ and groups of 9 male and 9 female beagle dogs in subacute experiments were given daily different dosage of NSCH, 160 ㎎/㎏/day (low dosage group), 400 ㎎/㎏/day (middle dosage group), 1,000 ㎎/㎏/day (high dosage group) once a day for 4 weeks by oral route according to the Established Regulation of Korea Food and Drug Administration (1996. 4. 16). LD_50 value for beagle dogs was more than 2,000 ㎎/㎏ per oral for both male and females. In animals administered with NSCH, there were neither dead animals nor significant changes of body weights. In addition, no differences were found between control and treated groups in clinical sign, urinalysis, eye examination, hematology, serum chemistry, organ weight and other fingings. No histological lesions were observed in both control and treatment groups. Above data strongly suggset that NSCH in beagle dogs is considered to be safe.

다수의 냉동시스템에 의한 저온 저장고의 에너지 성능평가

김성만,윤종호,박영하,이의준,강동언,현명택 제주대학교 산업기술연구소 2000 尖端技術硏究所論文集 Vol.11 No.1

The cool storage system(CSS) is a specific building that is required only cooling load without heating during the whole year. CSS uses a electric energy which is very expensive and is required much amount of energy due to temperature differences between interior and ambient temperatures. The purpose of this study is to assess the energy performance of the multi-unit cooler operating control system. For the prediction of its performance, two types of test cell are installed at Korea Institute of Energy Research (KIER). First cell is equiped with one large unit cooler, and second cell consists of two small unit coolers. According to the results of experiments using these cells, the multi-unit cooler operating control system is found to be effective in energy saving and beneficial for storage due to much less deviation of temperature.

다수의 냉동시스템에 의한 저온 저장고의 에너지 성능평가

강동언,김성만,운종호,이의준,박영하,현명택 濟州大學校 産業技術硏究所 2000 산업기술연구소논문집 Vol.11 No.1

The cool storage system(CSS) is a specific building that is required only cooling load without heating during the whole year. CSS uses a electric energy which is very expensive and is required much amount of energy due to temperature differences between interior and ambient temperatures. The purpose of this study is to assess the energy performance of the multi-unit cooler operating control system. For the prediction of its performance, two types of test (KIER). First cell is equiped with one .are installed a t Korea Institute of Energy Research large unit cooler. and second cell consists of two small unit coolers. According to the results of experiments using these cells. the multi-unit cooler operating control system is found to be effective in energy saving and beneficial for storage due to much less deviation of temperature.

비글개에서 신우황청심원의 급성 및 아급성독성시험

권오경,성하정,곽형일,방명주,신대희,이진영,박대규,정규혁,윤효인,조명행 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

Single and 4 weeks oral administration of New Woohwangchungsimwon (NWCH) which was used l-muscone as substitutive material of musk, to beagle dogs of both sexes were performed to investigate both acute and subacute toxicity. Beagle dogs(3 males and 3 females) in acute experiments were administered orally with single dose of 2,000 ㎎/㎏ and groups of 9 male and 9 female beagle dogs in subacute experiments were given daily different dosage of NWCH, 160 ㎎/㎏/day(low dosage group), 400 ㎎/㎏/day (middle dosage group), 1,000 ㎎/㎏/day (high dosage group) once a day for 4 weeks by oral route according to the Established Regulation of Korea Food and Drug Administration (1996. 4. 16). LD_50 value for beagle dogs was more than 2,000 ㎎/㎏ per oral for both male and females. In animals administered with NWCH, there were neither dead animals nor significant changes of body weights. In addition, no differences were found between control and treated groups in clinical sign, urinalysis, eye examination, hematology, serum chemistry, organ weight and other fingings. No histological lesions were observed in both control and treatment groups. Above data strongly suggset that NWCH in beagle dogs is considered to be safe.