비만환자의 전기지방분해침 시술 후 허리둘레 감소에 대한 임상적 고찰

김정환 ( Jeong Hwan Kim ),고연석 ( Youn Seok Ko ),이정한 ( Jeong Han Lee ),원재균 ( Jea Kyun Won ),신병철 ( Byung Cheul Shin ),권영달 ( Young Dal Kwon ),송용선 ( Yung Sun Song ) 한방재활의학과학회 2005 한방재활의학과학회지 Vol.15 No.3

Objectives : To see if there were any differences in waist circumference reduction when certain variable such as age, sex, BFR(BFR; Body Fat Rate), PIBW(PIBW; Percent Ideal Body Weight), WHR(WHR; Waist Hip Ratio) and BMI(BMI; Body Mass Index) were given. Methods : Among outpatients who admitted to department of Oriental Rehabilitation Medicine, Jeon-Ju Oriental Medicine Hospital, Won-kwang University from August 2003 to september 2004, 89 obese patients were chosen and electroacupunture was practiced to these groups in order to treat abdominal obesity and waist circumference reduction. Results : There were no significant correlation between sex, age and waist circumference reduction. Compared to groups who had shown lower BMI, higher BMI groups have shown greater change in waist circumference reduction. As BFR increase waist circumference reduction tended to increase as well. Conclusions : From the above results, higher BMI groups had shown greater change in waist circumference reduction compared to lower BMI groups.

연령이 증가함에 따른 흰쥐 망막의 막대세포, 원뿔세포 및 바깥얼기층의 미세구조 변화

고정식,한종원,양남길,안의태 대한해부학회 1992 Anatomy & Cell Biology Vol.25 No.3

뼈흡수유도호르몬이 ROS17/2.8세포로부터 Nitric Oxide 형성에 미치는 영향

고선일,김민성,한원정,김세원,김정근 대한구강악안면방사선학회 2005 Imaging Science in Dentistry Vol.35 No.3

Purpose : We performed the present study to investigate whether osteotropic hormomes play roles on the nitric oxide (NO) production in culture of ROS17/2.8 osteoblastic cells. Materials and Methods : The osteoblastic cell line ROS17/2.8 cells were cultured in F12 medium supplemented with 5% fetal bovine serum (FBS) at 37?C in a humidified atmosphere of 5% CO₂ in air. ROS17/2.8 cells were plated in 96-well plates at a density of 2-3×10³ cells/well and grown to confluence. Then the cells were pretreated with osteotropic hormones (parathyroid hormone (PTH) 20-500 ng/mL, 1,25 dihydroxycholecalciferol (1,25[OH]₂D₃) 1-100 nM; prostaglandin E₂ (PGE₂) 20-500 ng/mL) in the medium supplemented with 0.4% FBS for 72 hours and the cells were treated with cytokines (TNFα and IFNγ) in phenol red-free F12 medium for an additional 48 hours. NO synthesis was assessed by measuring the nitrite anion concentration, the reaction product of NO, in the cell culture medium using Griess reagent. Results : PTH and 1, 25[OH]₂D₃ pretreatment induced a significant increase in NO production in the presence of TNFα and IFNγ. PGE₂ slightly induced NO production compared to the control group. But, PGE2 pretreatment did not affect in NO production in the presence of TNFα and IFNγ. Conclusions : These results suggest that the actions of osteotropic hormones in bone metabolism may be partiallymediated by NO in the presence of cytokines.

과도 절삭에서의 절삭 공정 시뮬레이션

고정훈,조동우,윤원수,김주한 한국공작기계학회 2001 한국공작기계학회 춘계학술대회논문집 Vol.2001 No.-

In most of the existing mechanistic models, the cutting process simulation is often restricted to a single path machining operation under a fixed cutting condition. Complex cutting processes such as die or mold manufacturing, however, are performed under two- or three-dimensional multiple tool paths. Since the tool paths in CNC machining are composed of line and arc segments, transient cuts are frequently occured due to the multiple paths. Even in steady cuts, the width of cut is varied with each segment. In this regard, this paper deals with the development of process simulation system for transient cuts, where continuously changing cutting configuration is computed, and then the cutting forces are predicted.

Plyometric Training과 비타민 C 투여가 남고생의 체력, 혈중 젖산, 암모니아 및 젖산탈수소효소에 미치는 영향

장준원,김세종,이현미,서혜림,조현숙,고정림,염종우,예정복,백영호 釜山大學校 附設 體育科學硏究所 2003 體育科學硏究所 論文集 Vol.19 No.-

The purpose of this was to examine the effect of plyometric training and vitamin C ingestion on physical fitness and fatigue substance....

연령에 따른 흰쥐 망막 속얼기층의 미세구조 변화

오원영,고정식,양남길,안의태,박경호 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.2

이 실험은 연령에 따른 흰쥐 망막 속얼기층의 미세구조적 변화를 관찰하기 위하여 시행하였다. 실험동물로는 생후 3개월(어린 흰쥐), 12개월(젊은 흰쥐) 및 30개월(늙은 흰쥐)된 Fisher계통 344흰쥐를 사용하였다. 각 동물은 4% chloral hydrate로 마취한 후 2.5% glutaradehyde-1.5% paraformaldehyde로 관류 고정한 다음, 안구를 적출하여 톱니둘레를 다라 절단한 후 시신경원반외측의 일정부위에서 망막조직을 떼어내었다. 떼어낸 조직은 2.5% glutaradehyde- 1.5% paraformaldehyde 고정액에 다시 고정한 후, 1% osmium tetroxide액에 또 다시 고정하였다. 고정된 조직은 ethyl alcohol과 acetone으로 탈수한 후 araldite혼합액에 포매하였다. 포매된 조직은 LKB-V ultratome으로 얇은 절편을 작성하여 uranyl acetate와 lead citrate로 염색한 후 JEM 100CX-Ⅱ 전자현미경으로 관찰하여 다음과 같은 결론을 얻었다. 1. 무축삭신경원의 세포질돌기는 세포질의 전자밀도, 연접소포의 종류 및 양에 따라 4종류 구분되었다. 2. 속얼기층에서 관찰되는 신경연접의 형태는 연접리본복합체외에 4종류의 일반신경연접을 관찰할 수 있었으나, 연령증가에 따른 연접형태의 미세구조적 변화는 관찰할 수 없었다. 3. 연령이 증가함에 따라 부챗살아교세포의 상대적 영역이 넓어졌다. 4. 연령이 증가함에 따라 무착삭신경원과 신경절세포의 가지돌기안에 수초구조, 용해소체, 잔여소체가 자주 관찰되었다. 이상의 결과를 종합해 보면 흰쥐의 망막속얼기층은 연령이 증가하면 무축삭신경원과 신경절세포는 기능이 점점 약해지므로 부챗살아교세포의 영역이 상대적으로 더 넓어진다고 생각된다. Morphological differences among the inner plexiform layers at different age groups have been studied in young(three months old), adult(twelve months old) and old(thirty months old) Fisher stain 344 rats. Under anethesia, 2.5% glutaraldehyde- 1.5% paraformaldehyde solution was perfused through the heart. Pieces of the tissue taken from the posterior region of the retina were fixed in 2.5% glutaraldehyde- 1.5% paraformaldehyde, and in 1% osmium tetroxide followed by fixation embedded in aralditse mixture. Ultrathin seciton were statined with uranyl acetate and lead citrate solution and were observed with JEM 100CX-II electron microscope. The results were as follows: 1. Four types of amacrine cells were found according to their electron densities, and kind and amount of the synaptic vesicles, were found, i.e., dark amacrine cell with clear vesicles(Al cell), light amacrine cell with clear vesicles(A2 cell), dark amacrine cell with clear and granular vesicles(A3 cell) and light amacrine cell with clear and granular vesicles(A4 cell) 2. In the inner plexiform layer, there appear two types of synapses : 1) synaptic ribbon complex and 2) conventional synapses. Conventional ones exhibited four different types, showing, i.e., prominent presynaptic density(type 1), prominent postsnaptic density(type 2), prominent pre-and postsynaptic densities(type 3), and lesser prominent pre-and postsynaptic densites. 3. Territories of the Muller cells were expanded during aging. 4. Myelin figures, lysosomes, lipofuscin granules and lipid droplets were frequently observed in the amacrine and ganglion cells of the adult and old rats. From the above results, it was suggested that functions of the bipolar, amacrine and ganglion cells were diminished gradually, whereas territories of the Muller cells were expanded during aging.

중금속물질이 생쥐 신장사구체에 미치는 영향에 대한 미세구조 연구

김주원,고정식,양남길,안의태,박경호 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.2

본 실험은 중금속물질이 섭취되었을 때 신장사구체의 미세구조적 변화를 알아보기 위하여 시행하였다. 실험동물로는 체중 25gm내외의 성숙한 마우스(IRC)를 사용하였는데 실험군은 염화제이수은(HgCl₂)을 2mg/kg, 5mg/kg, 10mg/kg씩, 중크롬산칼륨(K₂Cr₂O7)을 5mg/kg, 10mg/kg, 20mg/kg씩 피하주사하여 사용하였다. 각 실험군은 주사 후 6시간, 3일, 2주일에 희생시킨 후 신장조직을 떼어내어 2.5% glutaraldehyde- 1.5% paraformaldehyde혼합액(0.1M Millonig's phosphate buffer, pH 7.3)에 다시 고정한 다음, 1% osmium tetroxide(0.1M Millonig's phosphate buffer, pH 7.3)에 다시 고정하였다. 고정된 조직은 ethyl acohol과 acetone으로 탈수하여 araldite 혼합액에 포매하였으며 포매된 조직은 LKB-V Ultramicrotome으로 얇은 절편을 만들어 uranyl acetate와 lead citrate액으로 염색한 후 JEM 100CX-Ⅱ전자 현미경으로 관찰하여 다음과 같은 결론을 얻었다. 1. 사구체기저막의 경우, 염화제이수은 투여군은 5mg/kg, 10mg/kg투여군에서, 중크롬산칼륨 투여군은 10mg/kg, 20mg/kg투여군에서 실험초기(6시간과 3일)에 사구체기저막의 전자밀도가 다소 불규칙하고 혹 모양의 물질이 기저막에 침착되었으나 2주일군에서 거의 정상군의 것에 비하여 별다른 변화를 볼 수 없었다. 2. 내피세포의 경우, 염화제이수은 투여군은 2mg/kg, 5mg/kg, 10mg/kg, 투여군에서, 중크롬산칼륨투여군은 10mg/kg, 20mg/kg투여군에서 실험초기( 6시간군과 3일 )에 내피세포 내에 공포와 myelin구조가 자주 관찰되었다. 3. 문어발세포의 경우, 염화제이수은 투여군은 2mg/kg, 5mg/kg, 10mg/kg 투여군에서, 중크롬산칼륨투여군은 10mg/kg, 20mg/kg 투여군에서 실험초기( 6시간군과 3일) 에 팽창된 과립형질내세망의 수조와 납작한 Golgi복합체 수조가 관찰되었다. 4. 혈관사이세포의 경우, 염화제수은 투여군은 2mg/kg, 5mg/kg, 10mg/kg 투여군에서, 중크롬산칼륨 투여군은 10mg/kg, 20mg/kg투여군에서 실험초기( 6시간군과 3일)에 기질내에 전자밀도가 높은 침착물이 관찰되었으며 혈관사이세포의 세포돌기가 내피세포 쪽으로 돌출되어 내피세포의 세포질판에 의해 둘러싸여 있는 모습이 자주 관찰되었다. 이상의 결과를 종합해보면 마우스가 일정량의 염화제이수은 또는 중크롬산칼륨을 한번 섭취하면 급성사구체신염의 형태학적 특징을 보이나 2주일정도가 지나면 사구체의 경우에는 거의 정상상태로 회복하며, 염화제이수은이 중크롬산칼륨에 비하여 사구체에 더 많은 손상을 준다고 생각된다. This experiment was performed to study the Ultrastructural changes of the renal glomerulus, induced by heavy metallic agents. Healthy adult mice weighing 25gm each were divided into normal an dexperimental grouops. Mercuric chloride(2mg/kg, 5mg/kg, 10mg/kg) or potassium bichromate(5mg/kg, 10mg/kg, 2-mg/kg) were injected subcutaneously to the animals. Animals were sacrificed at 6 hours, 3 days and 2 weeks following the injections. Pieces of tissues were taken from renal corticies, prefixed with 2.5% glutaraldehyde- 1.5% paraformaldehyde(0.1M Millonig's phosphate buffer, pH 7.3), and followed by post-fixation with 1% osmium tetroxide(0.1M Millonig's phosphate buffer, pH 7.3) Fixed blocks were dehydrated with alcohols and acetone, embedded in araldite mixture, cut with LKB V-ultratome. The ultrathin sections stained with uranyl acetate and lead citrate were observed with a JEM 100CX-II electron microscope. The results were as follow: 1. Glomerular basal laminae of the experimental groups showed some alterations, especially in the earlier stage-(6 hours and 3 days) of mercuric chloride-(5mg/kg. 10mg/kg) and potassium bichromate-(10mg/kg, 20mg/kg) treated groups. Irregularity of the electron density and accumulation of electron dense materials were observed. But morphological changes occurred in the basal laminae are generally recovered to normal appearance within 2 weeks. 2. In the glomerular endothelial cells of the mercuric chloride-(2mg/kg, 5mg/kg, 10mg/kg) and the potassium bichromate-(10mg/kg, 20mg/kg) treated groups. a large number of vacuoles and some myelin figures were observed frequently, especially in the earlier grous-(6 hours and 3 days). 3. In the podocytes of the mercuric chloirde-(2mg/kg, 5mg/kg, 10mg/kg) and potassium bichromate-(10mg/kg, 20mg/kg) treated groups. swollen cistern of rough endoplasmic reticulum and flattened cistern of the Golgi complexes were observed in the earlier groups-(6 hours and 3 days). 4. In the mesangial cell of the mercuric chloride-(5mg/kg, 10mg/kg) and potassium bichromate-(10mg/kg, 20mg/kg) treated groups. electron dense deposits were seen within the matrix and protruding cytoplasmic processes of the cells were surrounded by the cytoplasmic plates of the endothelial cells. From the above results. it was concluded that. the mercuric chloride or potassium bichromate induces acute glomerulonephritis on the mice, but ultrastructural changes occurred in the glomerulus after injection of the heavy metallic agents were recovered to normal appearance within 2 weeks. Mercuric chloride was more harmful on the renal glomerulus than potassium bichromte.

호흡율 측정기를 이용한 폐수의 외부탄소원 이용가능성 평가

최은희,고주형,임정훈,부경민,김창원 부산대학교 환경문제연구소 2000 環境硏究報 Vol.18 No.-

In this research, we measured Readily Biodegradable COD(Ss) using respiration meter which measured OUR and calculated respiration rate due to calculate organic matter portion of external carbon sources because known organic matter analysis methods i.e. COD, BOD did not measure amount of organic matter which was used in denitrification. Based on the experimental results, leather wastewater had 40.3% Ss in TCOD and food fermentation liquid, alcoholic wastewater and petrochemical wastewater was 38%, 38% and 28.3% respectively. However in case of considering of flowrate and concentration, flood fermentation and alcoholic wastewater was revealed that they were effective external carbon sources

CCD카메라를 이용한 3차원 데이터의 획득 및 가공

김영동,고낙용,김아현,강원찬,정헌 조선대학교 에너지.자원신기술연구소 2003 에너지·자원신기술연구소 논문지 Vol.25 No.1

Accurate acquisition of surface geometries such as machined surfaces, biological surfaces, and deformed parts have been very important technique in scientific study an engineering, expecially for system design, manufacturing and inspection. Two camera method keeps accuracy more than double than mechanical method. In this paper, we describe the processes surface data of teeth for automatic processing, transformation that changes to coping data of possible numeric data form, and processed inlay teeth. As the result of the inlay manufacture, which can process with high resolution, therefore we construct the automatic processing system that depends on manual.

영남 방언과 호남 방언의 운율 비교 : 대화체 월의 운율을 중심으로

김차균,고광모,김주원,정원수 충남대학교 문리과대학 어문연구회 2000 語文硏究 Vol.34 No.-

In this article, we compared the prosody of Yeongnam and Honam dialect, which is tone dialect and semi-tone dialect respectively. The data is that of Changweon dialect of Gyeongnam Province and Damyang dialect of Jeonnam Province chiefly. In some case the data of Yecheon dialect of Gyeongbuk Province was also dealt. For analysis of colloquial sentences, we employed three-way analysis: First, 1062 Changweon and Damyang colloquial sentences were dealt in auditory method. Secondly, 20 sentences of two dialects and 30 words of Changweon dialect were dealt with experimental method. Thirdly, for comparison of toponymy, the tone of the station names was investigated. In this article, we analyzed sentential tone of two dialects. For that, distribution of tone pattern and reduction or weakening of tone pattern in a sentence was investigated. The descripton about tone reduction was firstly done in this study.