몇종의 세균과 Saccharomyces cerevisiae에 대한 식품첨가물의 향균 특성

이종수,오준세,김나미,금종화,이석건,Lee, Jong-Soo,Oh, Jun-Sei,Kim, Na-Mi,Keum, Jong-Hwa,Lee, Suk-Kun 배재대학교 자연과학연구소 1996 自然科學論文集 Vol.8 No.2

현재 식품첨가물로 많이 사용되고 있는 유기산과 안정제 및 색소 등의 각종 세균과 Sacch. cerevisiae 에 대한 향균 특성을 조사하였다. 산도 조절용으로 사용되고 있는 젖산, 사과산, 호박산 및 주석산은 L. acidophilus와 Sacch. cerevisiae에 대하여 향균성이 없었으나 B. subtilis등의 세균에 대하여는 향균성이 있었고 특히 사과산은 P. aeruginosa에 대하여 강한 향균력이 있었다.(최소생육저지농도 : 0.05%). 안정제로서의 알긴산과 펙틴은 B. subtilis, E. coli, P. aeruginosa에 대하여 비교적 강한 향균성을 보였고 L. acidophilus에 대하여는 향균성이 없었다. 황색 색소(홍화엘로우)와 적색 색소(Red powder-N)는 향균성이 없었고 표백제인 $NaHSO_3$의 세균에 대한 최소생육저지농도는 0.05%, Sacch. cerevisiae에 대하여는 0.5%로 향균성이 있었다. In order to survey the safety of some food additives, antimicrobial activity of acidulants, stabilizers, antioxidants, natural coloring materials and bleaching agents against 5 strains of bacteria and Sacch. cerevisiae were investigated by dilution method and minimal inhibitory concentration(MIC) method. Malic acid as acidulants displayed the effective antimicrobial activity in vitro against P. aeruginosa and its MIC is 0.05%. Alginic acid and pectin as stabilizer also displayed strong antimicrobial activity against B. subtilis, E. coli and P. aeruginosa, and tannin(antioxidants) and $NaHSO_3$ displayed antimicrobial activity against all bacteria tested. However gums(Arabia, Xanthan, Gua) and natural coloring materials(Hongwha Yellow, Red powder-N) were not affected to growth of bacteria and Sacch. cerevisiae.

정상 및 원추각막에서 각막 전, 후면의 융기 및 시상면만곡도의 비교분석

이종수,이승욱,이승민,이미현,Jong Soo Lee,Seung Wook Lee,Seung Min Lee,Mi Hyun Lee 대한안과학회 2012 대한안과학회지 Vol.53 No.10

Purpose: To compare changes of anterior and posterior corneal elevation, and sagittal curvature according to the severity of keratoconus and to compare differences between keratoconus and normal cornea. Methods: A total of 81 eyes diagnosed with keratoconus and 20 eyes of normal subjects were evaluated with a Pentacam Scheimpflug camera. The keratoconus eyes were divided into 3 groups according to mean keratometer (K): mild (K ≤ 47.0 diopters (D)), moderate (47.0 to 52.0 D), and severe (52.0 D≥). The following parameters were obtained to evaluate the correlation of keratoconus: corneal thickness, anterior and posterior corneal elevation, and sagittal curvature. Results: Out of 81 keratoconus eyes, 56 eyes were mild, 12 eyes were moderate, and 13 eyes were severe keratoconus. The mean central corneal keratometer, anterior and posterior corneal elevation, and sagittal curvature of the keratoconus eyes were 49.7 D, 22.07 μm, 38.16 μm, 52.76 D and the values increased statistically compared to the normal eyes. Furthermore, the values increased significantly with the severity of keratoconus. ROC curve analysis showed the estimated meaningful value for anterior and posterior corneal elevation and sagittal curvature of keratoconus for diagnosis; there were no diagnostic values for corneal thickness and refractive power. Conclusions: The index of 5.5 μm for mean anterior elevation, 12.5 μm for mean posterior elevation, and 44.5 μm for mean sagittal curvature using the PentacamⓇ are useful to diagnose keratoconus. Variation of anterior and posterior elevation, and sagittal curvature measured by PentacamⓇ are useful in understanding the process of keratoconus. J Korean Ophthalmol Soc 2012;53(10):1432-1437

익상편조직의 섬유모세포에 미치는 사이클로스포린의 영향

이종수,이승욱,이상준,김나미,Jong Soo Lee,Seung Wook Lee,Sang Jun Lee,Na Mi Kim 대한안과학회 2012 대한안과학회지 Vol.53 No.3

Purpose: To evaluate the response and cellular damage of cultured human pterygial cells according to the concentration and exposure time of topical cyclosporin. Methods: Human pterygial cells were exposed to a cyclosporin A concentrations of 0.1 μg/ml (0.0001%), 1 μg/ml (0.0001%), 10 μg/ml (0.001%), 100 μg/ml (0.01%), or 500 μg/ml (0.05%) for 5 or 10 minutes. An MTT-based colorimetric assay was performed to assess the metabolic activity of cellular proliferation, and a lactate dehydrogenase (LDH) leakage assay was used to determine cellular damage. The extra-cellular matrix of PIP, laminin and MMP were evaluated, and the measurement of pro-inflammatory cytokine, TNF-α and IL-1b. IL-6, IL-8 was performed using ELISA kits. Results: The pterygial cellular inhibitory effect of cyclosporin was similar to that of the control according to the concentration and exposure time (p > 0.05). Compared with the control, the level of LDH did not show a statistically significant difference between concentration and exposure time (p > 0.05). There was no significant difference of inhibitory effects by PIP, laminin, or MMP between the experimental and control groups (p > 0.05). The production of TNF-α and IL from the experimental pterygial cells due to the effect of cyclosporin was not significantly different from that of the control at a longer exposure time or stronger concentration (p > 0.05). Conclusions: The response of pterygial cells to topical cyclosporin A at concentrations less than 0.05% for less than 10 minutes of exposure time showed no prevention of pterygial recurrence. With regard to cellular damage, little effects on inhibition of PIP, laminin, MMP, IL, and TNF-α were observed compared with that of the control. J Korean Ophthalmol Soc 2012;53(3):466-472

겔형태 인공누액이 각막실질세포 및 결막상피세포에 미치는 영향

이종수,김은희,김수진,이지은,김나미,Jong Soo Lee,Eun Hee Kim,Soo Jin Kim,Ji Eun Lee,Na Mi Kim 대한안과학회 2010 대한안과학회지 Vol.51 No.12

Purpose: To evaluate the biological effects and cytotoxicity of gel-type artificial tears on human corneal keratocytes and conjunctival cells in vitro. Methods: Human corneal keratocytes and conjunctival epithelial cells were exposed to Soothe® and Systane® at variable concentrations. Evaluations were conducted through an MTT-based calorimetric assay to measure the metabolic activity and through a lactate dehydrogenase (LDH) assay to assess cellular damage. Apoptotic response was examined using fluorescent microscopy and flow cytometric analysis, and cellular morphologic results were evaluated with a transmission electron microscope. Results: The inhibitory effects of corneal keratocyte and conjunctival cell proliferations increased at higher concentrations and longer exposure times to Soothe® and Systane®. The LDH titers increased after Soothe® exposure, but showed no significant difference after Systane® exposure. Soothe® and Systane® treatments both produced fluorescence, representing apoptotic cells. In flow cytometry, the maximal apoptotic response was observed for both types of artificial tears, although Systane® showed less edema, as well as reduced cytoplasmic and nuclear cell degeneration compared to those of Soothe®. Conclusions: The apoptotic responses of Soothe® and Systane® are associated with inhibitory effects of human corneal keratocyte and conjunctival epithelial cell proliferations. To inhibit the cellular proliferation of human corneal keratocytes and conjunctival epithelial cells, Systane® may be less severe than Soothe® at higher concentrations and longer exposure times. J Korean Ophthalmol Soc 2010;51(12):1643-1651

잉어 내장중의 Mouse 치사성분 Cyprinol Sulfate의 분리 및 생리 활성

이종수,Lee, Jong-Soo 생화학분자생물학회 1994 한국생화학회지 Vol.27 No.5

양식산 잉어(Cyprinidae, Cyprinus carpio) 내장의 MeOH 추출물로부터 mouse의 치사성분을 분리 정제하였다. 이 성분은 HRFABMS로부터 분자식은 $C_{27}H_{48}O_{8}S$로 판명되었으며 NMR 등 각종 기기 분석 자료를 검토한 결과, 잉어과 어류의 담낭 중에 존재한다고 알려진 독특한 성분인 cyprinol sulfate($5{\alpha}-cholestane$ $3{\alpha}$, $7{\alpha}$, $12{\alpha}$, 26, 27-pentol, 26-sulfate)로 동정되었다. Cyprinol sulfate의 mouse치사량은 2.0~3.0mg/MU 이었고, 약한 용혈성($500{\mu}g/ml$)과 prolyl endopeptidase 활성 저해작용($IC_{50}$ > $200{\mu}g/ml$)을 나타내었으나 Bacillus subtilis와 Aspergillus niger에 대한 항균성이나 L1210 세포에 대한 독성은 나타내지 않았다. A toxic component to mice was isolated from the viscera of cultivated carp (Cyprinus carpio) as white powder. Molecular formula was obtained to be $C_{27}H_{48}O_{8}S$ on the basis of the HRFABMS data. NMR spectra of the isolated compound were corresponded with those of $5{\alpha}-cyprinol$ except the $C_{26}$ sulfated carbon. Therefore, it was identified as cyprinol sulfate ($5{\alpha}-cholestane$ $3{\alpha}$, $7{\alpha}$, $12{\alpha}$,26,27-pentol 26-sulfate). It showed toxicity to mice (2~3 mg/MU, i.p.), hemolytic activity ($500{\mu}g/ml$) and prolyl endopeptidase inhibition ($IC_{50}$ > $200{\mu}g/ml$), but, had no antibacterial, antifungal and cytotoxic activity to L1210 cells.

주거공동체에 대한 애착과 신뢰의 영향요인 분석 - 친밀권역(intimacy zone)의 회복을 위한 시각 -

이종수,Lee, Jong Soo 한국주거학회 2015 한국주거학회 논문집 Vol.26 No.1

Community is crucial for human beings not only because they are born with communal self but also because social cooperation is more efficient than competition in many cases. Human beings are defined as Homo communicus living in residential community which is important for the fulfillment of their primary needs. Residential community is an 'intimacy zone' where personal and emotional interactions are made. This study aims at examining the health of residential community in Korea by exploring the level of place attachment and neighborhood trust. Previous studies indicate that place attachment and trust are influenced by three dimensions such as residents' characteristics, house types and regional environment. This study conducted a nationwide questionnaire survey and it shows that place attachment moulds neighborhood trust. The results of path analysis demonstrates that place attachment and trust appear high in detached house areas. Age of residents also gives positive influence on the level of place attachment and neighbourhood trust. This result gives significant policy implications for the development of residential community in Korea. Residential community as an intimacy zone has been eroded and desperately needs to be revitalized. For this, more concern should be given to the issue of desirable size of residential community and the types of houses. Detached house area must be supported by governmental policies.

피기백 인공수정체를 이용한 백내장 수술 후의 잔존 굴절 이상의 교정 1예

이종수,김영희,박정열,김성일,Jong Soo Lee,MD,PhD,Young Hee Kim,MD,Jung Yul Park,MD,Sung Il Kim,MD 대한안과학회 2014 대한안과학회지 Vol.55 No.12

Purpose: To report a case of corrected residual refractive error after cataract surgery with sulcoflex piggyback intraouclar lens (IOL). Case summary: A 77-year-old man was diagnosed as having hypermature cataract in the right eye and his corrected visual acuity in the same eye was hand-motion before surgery. Refractive error was +9.0 diopter (D) 6 months after conventional cataract surgery in the right eye. The authors performed additional cataract surgery using a piggyback method inserting a +10.0 D IOL in the ciliary sulcus. Four weeks after surgery, his refractive error was -1.25 D, visual acuity increased to 120/200 and 60/200 (with and without correction, respectively). No complication was observed during follow-up time and the patient was satisfied with his results. Conclusions: Correction of residual refractive error after cataract surgery with sulcoflex piggyback IOL is safe and effective. J Korean Ophthalmol Soc 2014;55(12):1890-1894

미생물 일반 , 생리 및 대사 재래식 메주로부터 효모의 분리 , 동정 및 배양조건

이종수,이성훈,권수진,안철,유진영 ( Jong Soo Lee,Sung Hun Yi,Su Jin Kwon,Cheol Ahn,Jin Young Yoo ) 한국미생물생명공학회 1997 한국미생물·생명공학회지 Vol.25 No.5

Neisseria lactamica 2118이 생성하는 $\beta$-Galactosidase의 정제 및 성질

이종수,곽인영,김나미,Lee, Jong-Soo,Kwak, In-Young,Kim, Na-Mi 배재대학교 자연과학연구소 1991 自然科學論文集 Vol.4 No.-

p-Aminopheny1-$\beta$-D-thiogalactopyranoside agarose 친화성 크로마토그라피를 이용하여 Neisseria lactamica 2118이 생성하는 $\beta$-galactosidase를 정제한 후 몇가지 효소학적 성질을 조사 하였다. Neisseria lactamica 2118이 생성하는 $\beta$-galactosidase는 구성효소로서 lactose와 IPTG에 의하여 유도되지 않았다. 정제효소의 작용최적온도는 $35^{\circ}C$, pH는 7.5이었고 $50^{\circ}C$로 15~60분 처리시 약 80%의 활성이 유지되었으며 pH 6.0~9.0에서 안정 하였다. 또한 $Hg^(2+)$와 $Co^(2+)$에 의하여 그 활성이 저해 되었다. $\beta$-Galactosidase(EC 3.2.1.23) from pathogenic Neisseria lactamica 2118 was purified by p-aminopheny1-$\beta$-D-thiogalactopyranoside agarose affinity chromatography. Then some properties of the purified $\beta$-galactosidase were investigated.$\beta$-Galactosidase form Neisseria lactamica 2118 was constitutive enzyme, not induced by lactose and IPTG. Optimal activity was observed at $35^{\circ}C$ and pH 7.5 and the enzyme was stable at the range of pH 6.0-9.0 and at temperature below $50^{\circ}C$. The enzyme activity was inhibited by cations such as $Hg^(2+)$ and <Tex>$Co^(2+)$.

실사적인 3차원 물체 모델링 기법 개선

이종수,Lee, Jong-Soo 한국정보처리학회 2003 정보처리학회논문지B Vol.10 No.2

본 논문에서는 실사적인 3D 물체 모델링을 위해 제안된 기법들을 고찰하고, 보다 정확한 모델링을 위한 방안들을 제시하고 있다. F-행렬 추정기법의 개선과 스테레오 영상 평행화기법을 적용함으로 물체 모델링의 정확도를 높히는데 필수적임을 보여준다. In this paper, in-depth considerations are given to various techniques suggested for photographic 3D modelling and possible enhancements of the techniques are discussed. It is found that both the improvement of fundamental matrix estimation techniques and the stereo image rectification processing stage are necessary for more accurate 3D modelling.