활천을 여는 글 : 여교역자회전국연합회 신임회장 - 이군자 전도사 ( mini interview )

이군자 기독교대한성결교회 활천사 2002 활천 Vol.587 No.-

저질이 천연수중 구리와 카드뮴의 화학종 분포에 미치는 영향

이군자,박청길 한국환경과학회 1992 한국환경과학회지 Vol.1 No.2

In order to predict the distribution of chemical species of copper and cadmium in water, conditional stability constant and complexation capacity between copper or cadmium and natural aquatic sediment have been determined in a shallow lake in Haman, Kyungnam. Kinetic parameters were calculated by Langmuir isotherm equation. Conditional stability constant was log K_cdses=4.78 and log K_cdsed=4.45. Complexation capacity was 1.70×10 exp (-4) moles/g for copper and 5.54×10 exp (-4) moles/g for cadmium. Accuracy of experimental values of conditional stability constant was checked by comparing the calculated concentration of the metals with the measured one. Relatively good agreement between these values was obtained. Relative errors were 8.9% for copper and 6.5% for cadmium. Data of the measured conditional stability constant were put into data base of MINEQL computer program, and concentration of various chemical species of copper and cadmium in a model aquatic system was calculated. Aquatic sediment was associated with copper at the concentration of 10^-5M(0.059g/ℓ) and with cadmium at the concentration of 10^-6M(0.018g/ℓ), and it significantly influenced on the distribution of chemical species of the metals. This result showed that prediction of chemical species of the heavy metals in an aquatic system should be taken into account the influence of the sediment.

제 3회 국제간호학술대회 참석보고

이군자,Lee K J 대한간호협회 1981 대한간호 Vol.20 No.4

Mitomycin C투여에 의한 공막의 조직병리학적 연구

이군자 서울保健大學 1996 論文集 Vol.16 No.1

익상편 수술 후 재발 방지를 위하여 사용되고 있는 MMC를 안구의 성장이 활발한 어린 흰쥐에게 점안한 후 나타나는 공막의 조직학적 및 조작 화학적인 변화를 관찰하여 다음과 같은 결과를 얻었다. 1) 저농도(0.04mg/mL)의 MMC를 점안한 공막에서는 조직학적인 변화를 관찰할 수 없었으나, 고농도(0.1mg/mL) 의 MMC를 3일간 점안한 실험군과 7일간 점안한 실험군에서는 공막실질의 아교섬유의 간격이 상당히 벌어졌으며 이러한 조직 손상은 MMC 점안을 중지한 후에도 완전히 회복되지 않았다. 2) 고농도(0.lmg/mL)의 MMC를 점안한 실험군의 공막실질에서 alkaline phosphatase 활성은 약간 감소하였으며, MMC 투여 후 7일이 경과한 경우에도 효소활성이 회복되지 않았다. 3) 저농도(0.04mg/mL) 및 고농도(0.lmg/mL)는 MMC를 점안한 실험군의 공막실질에서 섬유모세포와 제I형 아교질 항체에 대한 반응은 대조군에 비하여 감소하였으며, MMC 점안 후 7일이 경과한 실험군에서도 제I형 아교질항체에 대한 반응은 회복되지 않았다. 4) 저농도의 MMC를 1회 점안한 실험군, 3일간 점안한 실험군, 접안 후 7일 경과군 및 고농도의 MMC를 1회 투여한 실험군의 공막실질에서는 Cu, Zn-SOD 활성이 관찰되었으나, 고농도의 MMC를 장기간 점안한 실험군과 점안 후 7일이 경과한 실험군에서는 활성이 관찰되지 않았다. 이상과 같은 결과로 MMC를 점안한 흰쥐의 공막실질에서는 일시적으로 Cu, Zn-SOD 의 활성이 증가하여 MMC에 의한 조직독성을 일부 줄일 수 있을 것으로 생각되나, MMC를 계속 점안한 경우에는 alkaline phosphatase 활성이 감소하고 아교섬유의 합성능력이 감소하여 공막실질의 아교섬유가 정상적인 판(lamella) 을 형성하지 못하여 공막괴사 등의 부작용이 나타나는 것으로 생각되며, 고농도의 MMC를 점안한 경우에는 점안을 중지한 후에도 공막에 조직독성이 나타나는 것으로 생각된다. Mitomycin C(MMC), widely used as an adjunct in the surgical treatment of pterygium, was instilled to the young rats to evaluate the histologic and histopathologic toxicity of the sclera. 1. Significant histologic changes were not detected in the lower dose of MMC(0.04mg/mL) treated groups, but lamella structure of sclera was irregular in the higher dose of MMC(0.1mg/ mL) treated groups. These structural changes were not recovered within 7days after ceasation of MMC instillation. 2. Alkaline phosphatase activity was decreased in the higher dose of MMC treated groups, and it was not recovered within 7 days after ceasation of MMC instillation. 3. Type I collagen and type I collagen synthetic activity of fibroblast were decreased in the lower and higher dose of MMC treated groups, and these changes were not recovered within 7 days after ceasation of MMC instillation. 4. Cu, Zn-SOD activity was increased in the lower dose of MMC treated groups and single application of higher dose of MMC, but Cu, Zn-SOD activity was not detected in the other higher dose of MMC treated groups. These results indicate that increase of Cu, Zn-SOD activity may reduce the MMC toxicity temporarily, but repeated MMC treatment changes the sclera irregular by decreasing biosynthesis of collagen in the fibroblast. These irregular lamella structure may lead to necrotizing scleritis.

京畿道 地域에서 自動車事故로 인한 死亡 및 負傷

李君子 서울大學校保健大學院 1975 公衆保健雜誌 Vol.12 No.2

This study was aimed to disclose the magnitudes and trends of deaths and injuries from motor vehicle accidents with the relations to several factors such as population, number of vehicles, roads's conditions and size of area. Raw data were collected from the various available sources of local government. Main data related to incidence, deaths and injuries were obtained from the records of Department of Police in Gyung-gi Province for 4 years period from 1971 to 1974. The following was summary of the results made with the analysis of the data collected: 1. The total events of motor vehicle accidents in Gyung-gi area for 4 years were 18,140 cases. The annual average rates of incidence, death and njury per 100,000 population were 124.5, 13.6 and 160.6 repectively. The above rates were higher in the urban area than in the rural area. On the other hand, the rates of incidence, death and injury tend to decrease annually. 2. The age specific rates of deaths and injuries per 100,000 population were 10.0 and 79.2, respcetively in the ages under 14, whereas 15.8 and 209.8, respectively in the ages above 15. Among total numbers of death and injured, the age group of under 14 constituted 27.7% and 18.6%, respectively. 3. The everage incidence of motor vehicle accidents per 1,000 vehicles registered was 297.7 events and the number of the death and the injured were 32.4 and 383.5 persons per 1,000 vehicles, respectively. 4. The average incidence, death and injury per 100,000 meters of road's length were 105.1 cases, 11.5 and 135.6 persons, respectively. The above averages were higher in the urban than in the rural areas. 5. The average incidence, death and injury per 100km²of area were 41.4 cases, 4.5 and 53.4 persons, respectively. These averages also were larger in the urban than in the rural areas. 6. Setting the values in 1971 as baseline index 100, population, number of vehicles and population density were steadily increased, but both of events and rates per population of incidence, death and injury were decreased in cases and rate per 100,000 population, and there were no definite changes in road's length and size of area. 7. The average numbers of the death and the injured from motor vehicle accidents were 109.0 and 1,289 persons per 1,000 accidents, respectively. On the other hand death versus injury was average 1:11.8. 8. The daily average of motor vehicle accidents in incidence was 12.4 events, that of death and injured were 1.4 and 16.0 persons, respectively. It Showed that the number of accidents was the greatest in July and August. 9. There were no definite differences in the incidence of motor vehicle accidents by the week days, but they happened somewhat more on Sunday and Monday. 10. The motor vehicle accidents occurred most frequently in the 12:00-18:00 o'clock by time. 11. The causes of motor vehicle accidents were classified 95.1% of drivers fault, 2.9% of pedestrians faults, 2.0% of poor maintenance.

韓國 小年非行에 對한 小考 : 豫防과 善導외 諸問題點

李君子 이화여자대학교 사회사업학과 1967 사회사업 Vol.- No.2

日本紀行 : 이웃나라 일본의 인상

이군자 경기대학 관광학회 1967 觀光學報 Vol.1 No.-

Mouse 신장으로부터 Ornithine Decarboxylase의 정제

이군자,조영동 한국생화학회 1992 BMB Reports Vol.25 No.5

자성 mouse의 피하층에 testosterone을 투여하여 ornithine decarboxylase의 활성을 증가시킨 후, 신장으로부터 ornithine decarboxylase를 황산암모늄 분별침전, DEAE-sephacel chromatography, hydroxylapatite chromatography, pyridoxamine-5'-phosphate agarose affinity chromatography를 이용하여 분리 정제하였다. 이 효소의 분자량은 SDS-polyacrylamide gel electrophoresis, Sephacryl S-200 gel chromatography와 ornithine decarboxylase의 효소활성 억제제인 14C-DFMO 을 효소와 결합시켜 얻은 autoradiography를 이용하여 52,000 dalton인 동일한 subunit으로 구성된 dimer임을 알 수 있었다. 정제된 효소의 ornithine에 대한 K_(m) 값과 V_(max) 값은 각각 167㎛과 5 nmol CO_(2)/h로 나타났고, pyridoxal-5-phosphate에 대한 K_(m) 값과 V_(max) 값은 각각 0.142㎛과 2.4nmol CO_(2)/h로 나타났다. 효소의 최적 pH 는 7.0, 최적 온도는 37℃이었으며, 정제된 ornithine decarboxylase는 polyamines(putrescine, spermidine, spermine)에 의해 효소 활성이 영향을 받지 않았다. 본 실험에서는 ornithine decarboxylase의 분리과정 중에서 ornithine decarboxylase 활성을 안정화시키는 물질을 발견하였다. Ornithine decarbosylase (ODC) was purified to homogenity from testosterone treated mice kidney by ammonium sulfate fractionation, DEAE-Sephacel chromatography, hydroxylapatite chromatography, pyridoxamine-5-phosphate-agarose affinity chromatography. The specific activity of ODC was 14,000 U/㎎, and purification fold was 1,180. The enzyme has a Mr. of about 110,000 dalton and is a dimer of subunit Mr. 52,000. The K_(m), for L-ornithine was 167㎛ and for pyridoxamine phosphate, 142 nM. ODC has an optimum pH of 7.0 and optimum temperature of 37℃. The purified enzyme was not inhibited by polyamine (putrescine, spermidine and spermine). During purification process, ODC stabilizing material was found.

감각자극이 미숙아의 체중, 스트레스호르몬 및 행동상태에 미치는 효과

이군자 한국간호과학회 1999 Journal of Korean Academy of Nursing Vol.29 No.2

염화 Cadmium 이 흰쥐 간장에 미치는 영향에 관한 전자현미경적 연구

강신덕,정호삼,이규식,이군자 漢陽大學校 環境科學硏究所 1992 環境科學論文集 Vol.13 No.-

실험동물은 본 실험실에서 순종교배에 의해 육성시킨 Wistar계 흰쥐를 사용하였으며, 순종교배에 의해 출생 제 7일의 신생 흰쥐 (체중 10g내외) 출생 제 40일된 흰쥐 (체중 120g내외)를 사용하였다. 신생 흰쥐와 성숙 흰쥐에 각각 cadmium chloride를 체중Kg당 3.75mg되게 생리적 식염수에 용해하여 0.2ml을 복강내에 투여한 후 3시간, 6시간, 12시간, 24시간 및 36시간 후에 각각 희생하고 개복하여 간장을 절취하였다. 간세포의 미세구조를 관찰하기 위해 조직은 4% glutaraldehyde에 전고정하였으며 10% osmium tetroxide로 후고정 후 초박절편을 제작하여 uranyl acetate 와 lead citrate로 이중 염색을 시행하였으며 전자현미경으로 관찰하여 다음과 같은 결과를 얻었다. 1. cadmium chloride 투여 신생 흰쥐의 간실질세포에서 골지체는 3시간군, 6시간군및 36시간군에서 소조가 위축되었다. 사립체는 3시간군에서 팽대되었고, 24시간군에서는 사립체롱이 소실되었으며 심한 팽대가 관찰되었고, 36시간군에서는 불규칙한 형태를 보이나 사립체롱은 정상적으로 관찰되었다. 조면내형질세망은 3시간 군에서 소조의 팽대와 일부 낭상화가 관찰되었으며 6시간군에서는 낭상화와 막부착 ribosome 과립의 탈락을 볼 수 있었고 24시간군과 36시간군에서는 대부분의 소조가 낭상화되었다. 2. cadmium chloride 투여 성숙 흰쥐의 간실세포에서 골지체는 3시간군에서 소조가 다소 팽대되었고 24시간군에서는 소조의 수가 감소되었다. 사립체는 6시간군에서 팽대되었고, 12시간군에서 불규칙한 형태를 보였으나 36시간군에는 대조군과 유사한 구조로 관찰되었다. 조면내형질세망은 6시간군, 12시간군 및 24시간군에서 소조의 팽대, 낭상화 및 막부착 ribosome과립이 탈락을 관찰할 수 있었고 36시간군에서는 대조군과 유사한 구조로 관찰되었다. Phagosome이 3시간군과 6시간군에서 다수 관찰되었고 12시간군, 24시간군 및 36시간군에서는 자가용해소체와 잔여소체를 볼 수 있었으며 특히 36시간군에서 증가되었다. 이상과 같은 결과로 미루어 cadmium chloride를 신생 흰쥐와 성숙 흰쥐에 투여하였을 때 미세구조상 신생 흰쥐 간세포의 미세구조는 투여후 시간이 지남에 따라 정상구조와 유사한 상태로 회복되는 것으로 사료된다. It is known that cadmium exposure to human results in very harmful adn potenteally toxic to liver and kideny. Therefore, the authors were undertaken to pursue the acute effects of cadmium chloride on the ultrastructures of rat hepatocytes, The 7 days old newborn and adult Wistar rats(40 days old) were used as experimental animals. Both new born and adult rats were injected intraperitoneally with 3.75 mg/kg cadmium chloride, 0.2ml(diluted with 0.9% NaCl solution). The sections of the experimethtal animals were made and observed by electron microscope. The results obtained were as follows. 1. In the hepatocytes of cadmium chloride treated newborn rats, cisternae of Golgi complexes are atrophied in the 3hr, 6hr. and 36hr. groups. The mitochondria are hypertrophied in 3hr. group. Cristae of mitochondria are reduced and swollen in 6hr. group. The cisternae of rough endoplasmic reticulum are dilatated and partly sacculated in 3hr. group and the associated ribosome granules are datached. In the 24hr. and 36hr. group, the most cisternae are sacculated. 2. In the hepatocyte of cadmium chloride treated adult rats, the cisternae of Golgi complexes are slightly dilatated and reduced in 24hr. group. Mitochondria are hypertrophied in 6hr. group and seen irregular shapes in 12hr. group but in 36hr. group, most mitochondria are intact as well as that of control rat groups. Rough endoplasmic reticulum showes dilatation, sacculation and detachment of associated ribosome granules from cisternase in 6hr. 12hr. and 24hr. group. But in 36hr. group. the fine structures of rough endoplasnmic reticulum are intact. The secondary lysosomes, phagosomes, are observed in 3hr. and 6hr. groups and numerous autophagic vacuoles and lipofucin granules are seen in 12hr. and 24hr. gruop. The large lipofucin granules are increased in 36hr. group. Consequently it is suggested that cadmium chloried made several toxic effects to hepatocytes of newborn and adult rat, the damage of hepatocyte increased in adult rats and newborn rat.