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황소련,조지훈,신경민,장윤영,김지연,여경욱,김형아,허용,Hwang, So Ryeon,Jo, Ji Hoon,Shin, Kyeong Min,Jang, Yun Young,Kim, Ji Youn,Yeo, Kyeong Uk,Kim, Hyoung Ah,Heo, Yong 한국환경보건학회 2012 한국환경보건학회지 Vol.38 No.6
Objectives: This study was undertaken in order to evaluate a potential mechanism involved in gastro-intestinal problems observed in autistic subjects and uses an animal model of autism investigation. Methods: BTBR T+tf/J, a mouse strain with typical socio-behavioral characteristics of autistic subjects and FVB mice with highly social behaviors as the control strain were used. Both genders of mice aged three weeks and six months were used from four separate litters for each strain. Serum was prepared following cardiac puncture, and mesenteric lymph nodes were collected for in vitro stimulation and enumeration of major immune cell proportion. Results: The level of serum IgA was significantly enhanced in six-month-old BTBR mice compared with three-week-old BTBR, which was not observed with the FVB control mice. The serum IgE level was also higher among BTBR mice than among age-sex matched FVB mice, respectively. Considering the ratio of interleukin-4 vs interferon-gamma production from mesenteric lymph node T cells, skewedness toward type-2 reactivities was observed. In addition, the proportion of B cells in mesenteric lymph nodes was significantly higher in BTBR mice than in FVB mice. Conclusion: Upregulation of mucosal immunity related with enhanced type-2 immune reactivity observed in BTBR mice could be involved with the etiology of gastro-intestinal abnormalities in autism.
경북 일부지역 유흥 관련 실내환경에서 간접흡연 지표의 니코틴 농도 평가
김순신,홍가연,김동건,황소련,우병렬,안호기,양원호 한국실내환경학회 2012 한국실내환경학회지 Vol.9 No.1
xposure to environmental tobacco smoke (ETS) could adversely affect health. The aim of this study was to quantify the contribution of ETS exposure in nonsmokers of entertainment facilities. We simultaneously measured nicotine and nitrogen dioxide (NO₂), which are known as indicators of ETS, concentrations in indoor internet cafe, billiard, karaoke, bar and restaurant, and estimated exposure level of other harmful agents occurred from tobacco smoking. Mean nicotine concentration (10.57±2.53 ㎍/㎥) of internet cafe was the highest comparing to other facilities, whereas mean concentration of restaurant where was non-smoking area was 0.28±0.08 ㎍/㎥. There was statistically not correlated between NO₂and nicotine concentrations in entertainment facilities. Therefore, the use of NO₂ concentration as indicator of ETS exposure may not be available. To date, there are no standards about each agent occurred from ETS. Consequently administrative control and regulation, and further researches in relation to ETS exposure should be needed.
High-content analysis of in vitro hepatocyte injury induced by various hepatotoxicants
Nga T. T. Tham,황소련,방지현,이희수,박영일,강석진,강환구,김용상,구현옥 대한수의학회 2019 Journal of Veterinary Science Vol.20 No.1
In vitro prediction of hepatotoxicity can enhance the performance of non-clinical animal testing for identifying chemical hazards. In this study, we assessed high-content analysis (HCA) using multi-parameter cell-based assays as an in vitro hepatotoxicity testing model using various hepatotoxicants and human hepatocytes such as HepG2 cells and human primary hepatocytes (hPHs). Both hepatocyte types were exposed separately to multiple doses of ten hepatotoxicants associated with liver injury whose mechanisms of action have been described. HCA data were obtained using fluorescence probes for nuclear size (Hoechst), mitochondrial membrane potential (TMRM), cytosolic free calcium (Fluo-4AM), and lipid peroxidation (BODIPY). Cellular alterations were observed in response to all hepatotoxicants tested. The most sensitive parameter was TMRM, with high sensitivity at a low dose, next was BODIPY, followed by Fluo-4AM. HCA data from HepG2 cells and hPHs were generally concordant, although some inconsistencies were noted. Both hepatocyte types showed mild or severe mitochondrial impairment and lipid peroxidation in response to several hepatotoxicants. The results demonstrate that the application of HCA to in vitro hepatotoxicity testing enables more efficient hazard identification, and further, they suggest that certain parameters could serve as sensitive endpoints for predicting the hepatotoxic potential of chemical compounds.
연구논문 : 정각질세포주의 피부감작물질 검색 관련 효용성 분석
김소남 ( So Nam Kim ),여경욱 ( Kyeong Uk Yeo ),황소련 ( So Ryeon Hwang ),조지훈 ( Ji Hoon Jo ),손수정 ( Soo Jung Sohn ),염영나 ( Young Na Yum ),이용경 ( Yong Kyung Lee ),허용 ( Yong Heo ) 한국동물실험대체법학회 2011 동물실험대체법학회지 Vol.5 No.1
Alternative methods using various human or animal cell lines are under development to classify chemical ingredients to skin sensitizing or irritant chemicals. Keratinocyte cell line could be a good target cell, in that the cells are the first contact point for chemical absorption through skin, and immune reactions including inflammation are occurred through interactions among keratinocyte, Langerhans` cell, and T cells. This study introduced current research activities on development of alternative methods using keratinocyte cell lines for identifying skin sensitizers. In addition, a good laboratory practice was introduced to perform serial passage culture of HaCaT human keratinocyte cell line. Furthermore, optimal cell density and culture duration were demonstrated to determine both intracellular and extracellular levels of IL-1α, IL-6, IL-8, and IL-18 from HaCaT cells.
피부감작능 평가를 위한 BrdU-FCM 국소림프절시험법 기법 이전을 위한 제 조건
여경욱 ( Kyeong Uk Yeo ),김지연 ( Ji Youn Kim ),황소련 ( So Ryeon Hwang ),임경민 ( Kyung Min Lim ),정경미 ( Kyung Mi Jung ),장원희 ( Won Hee Jang ),허용 ( Yong Heo ) 한국동물실험대체법학회 2012 동물실험대체법학회지 Vol.6 No.1
Local lymph node assay (LLNA) using mice has been adopted as an alternative model for testing skin sensitization to chemicals. Modification of LLNA was recently reported through combination of BrdU incorporation and flow cytometric analysis, which was designated as LLNA:BrdU-FCM. We introduce how LLNA:BrdU-FCM could be successfully established in a naive laboratory where the method was never experienced. Nine week old female BALB/c mice, 5 mice per group, were used and grouped into non-treatment, BrdU only-treatment, acetone-olive oil (AOO) vehicle-treatment, and 5, 10, 25% α-hexylcinamaldehyde (HCA) positive control. BrdU solution was injected intraperitoneally one day after three consecutive application of AOO or HCA on dorsum area of both ears. Auricular lymph node was collected from both ears one day after the BrdU injection. Thereafter flow cytometric staining procedures were followed. Various parameters related with calculation of stimulation index were analyzed including number of lymphocytes, BrdU incorporation%, and number of BrdU incorporated lymphocytes. The critical procedures for successful establishment were as followings; quality control of every quantitative apparatus, appropriate application of test compounds on dorsum of mouse ear, precise injection of BrdU, accurate preparation of single cell suspension and counting of lymph node cells, and experienced operation of flow cytometer. Face-to-face training from an experienced lead laboratory and preparation of very detailed standard operational procedures could play the most important role for the establishment. Based on Performance Standards for Assessment of Proposed Similar or Modified Test Methods by OECD, the LLNA:BrdU-FCM could be a good alternative method for screening skin sensitizers.
김소남 ( So Nam Kim ),여경욱 ( Kyeong Uk Yeo ),황소련 ( So Ryeon Hwang ),조지훈 ( Ji Hoon Jo ),손수정 ( Soo Jung Sohn ),염영나 ( Young Na Yum ),이용경 ( Yong Kyung Lee ),허용 ( Yong Heo ) 한국동물실험대체법학회 2011 동물실험대체법학회지 Vol.5 No.1
Alternative methods using various human or animal cell lines are under development to classify chemical ingredients to skin sensitizing or irritant chemicals. Keratinocyte cell line could be a good target cell, in that the cells are the first contact point for chemical absorption through skin, and immune reactions including inflammation are occurred through interactions among keratinocyte, Langerhans` cell, and T cells. This study introduced current research activities on development of alternative methods using keratinocyte cell lines for identifying skin sensitizers. In addition, a good laboratory practice was introduced to perform serial passage culture of HaCaT human keratinocyte cell line. Furthermore, optimal cell density and culture duration were demonstrated to determine both intracellular and extracellular levels of IL-1α, IL-6, IL-8, and IL-18 from HaCaT cells.