정상 정자 소견을 나타내는 불임 부부에서 일반적인 체외수정과 세포질내 정자주입술을 병행하는 분할 수정법의 효용성

홍승범,박동욱,신미라,최수진,이선희,송인옥,전진현,Hong, Seung-Bum,Park, Dong-Wook,Shin, Mi-Ra,Choi, Su-Jin,Lee, Sun-Hee,Song, In-Ok,Jun, Jin-Hyun 대한생식의학회 2007 Clinical and Experimental Reproductive Medicine Vol.34 No.4

목 적: 본 연구에서는 일반적인 체외수정과 세포질내 정자주입술을 병행하여 시행하는 분할 수정법의 효용성을 정상적인 정자 소견을 나타내는 비남성요인 불임 환자의 체외수정 및 배아이식술에서 살펴보고자 하였다. 연구방법: 제일병원 아이소망센터에서 정자 소견상 결함이 없는 비남성요인 불임 환자에게 분할 수정법을 이용한 505주기의 임상 결과를 후향적으로 분석하였다. 획득된 난자는 무작위로 나누어 일반적인 체외수정이나 세포질내 정자주입술을 이용하여 수정을 시켰다. 수정란은 이식할 때까지 2$\sim$5일간 배양하였고, 잉여의 수정란과 배아는 동결보관하여 동결-융해 이식에 사용하였다. 수정 방법에 따른 임상 결과를 통계학적인 방법으로 비교하였다. 결 과: 획득된 난자의 수정율은 분할 수정법을 시행하였을 때 일반적인 체외수정보다 세포질내 정자주입술에서 통계적으로 유의하게 높게 나타났다 (52.9$\pm$28.0% vs 62.5$\pm$22.3%, p<0.01). 전체적인 수정의 실패는 분할 수정법을 시행한 505주기 중에서 단지 2주기에서만 (0.4%) 나타났으며, 수정 실패와 0$\sim$30% 이하의 수정률을 나타내는 빈도는 세포질내 정자주입술에서 일반적인 체외수정보다 통계적으로 유의하게 낮았다 (1.1% and 7.5% vs 8.5% and 22.0%, p<0.01). 분할 수정주기에서의 신선 배아와 동결-융해 배아이식 후 분만율은 각각 40.0% (185/462)와 35.0% (55/157)였으며, 일반적인 체외수정이나 세포질내 정 자주입술로 얻어진 배아의 착상률과 분만율은 유의적인 차이가 나타나지 않았다. 결 론: 결론적으로 비남성요인 불임 환자의 보조생식술에서 분할 수정법이 수정 실패와 낮은 수정율을 방지하고 성공적인 임상 결과를 제공할 수 있는 효과적인 방법으로 사료된다. Objective: To evaluate the efficacy of split insemination method in treatments for non-male factor infertility. Method: Laboratory and clinical data were collected from 505 cycles of split insemination during 2002$\sim$2005 in our center. The subjects were non-male factor infertility such as endometriosis, tubal, uterine, PCOS and idiopathic infertility without any sperm defects. Retrieved oocytes were randomly divided, and inseminated by conventional IVF or ICSI. Fertilized zygotes were cultured for 2$\sim$5 days to ET date, and surplus zygotes and embryos were frozen for subsequent frozen-thawed ET cycles. Clinical outcomes according to insemination method were compared by statistical analysis. Results: The overall fertilization per retrieved oocytes was significantly higher in ICSI than that of conventional IVF in sibling oocytes (62.5$\pm$22.3% vs 52.9$\pm$28.0%, p<0.01). Total fertilization failure occurred only in 2 of 505 cycles (0.4%) in split insemination cycles. Incidence of fertilization failure and poor fertilization rate less than 30% by ICSI were significantly lower than those of conventional IVF (1.1% and 7.5% vs 8.5% and 22.0%, p<0.01). Delivery rates after transfer of fresh and thawed embryos from split insemination cycles were 40.0% (185/462) and 35.0% (55/157), respectively. There was no significant difference in the implantation and delivery rates of ET with embryos from conventional IVF or ICSI. Conclusion: Taken together, the split insemination method improves poor fertilization rates resulting in successful clinical outcomes and thus could be used for non-male factor infertile couples in human ART program.

흡수성 차폐막의 조직반응에 관한 비교연구

홍승범,권영혁,이만섭,허익,Hong, Seung-Bum,Kwon, Young-Hyuk,Lee, Man-Sup,Herr, Yeek 대한치주과학회 2002 Journal of Periodontal & Implant Science Vol.32 No.3

The purpose of this study is to evaluate histologically the resorption and tissue response of various resorbable collagen membranes used for guided tissue regeneration and guided bone regeneration, using a subcutaneous model on the dorsal surface of the rat. In this study, 10 Sprague-Dawley male rats (mean BW 150gm) were used and the commercially available materials included acellular dermal matrix allograft, porcine collagen membrane, freeze-dried bovine dura mater. Animals were sacrificed at 2,6 and 8 weeks after implantation of various resorbable collagen membranes. Specimens were prepared with Hematoxylin-Eosin stain for light microscopic evaluation. The results of this study were as follows: 1. Resorption : Inner portion of porcine collagen membrane was resorbed a lot at 6 weeks, but its function was being kept for infiltration of another tissues were not observed. Freeze-dried bovine dura mater and acellular dermal allograft were rarely resorbed and kept their structure of outer portion for 8 weeks. 2. Inflammatory reactions : Inflammatory reaction was so mild and foreign body reaction didn't happen in all of resorbable collagen membranes, which showed their biocompatibility. 3. In all of resorbable collagen membranes, multinuclcated giant cells by foreign body reactions were not observed. Barrier membranes have to maintain their function for 4-6 weeks in guided tissue regeneration and at least 8 weeks in guided bone regeneration. According to present study, we can find all of the resorbable collagen membranes kept their function and structure for 8 weeks and were rarely resorbed. Foreign body reaction didn't happen and inflammatory reaction was so mild histologically. Therefore, all of collagen membranes used in this experiment were considered proper resorbable membranes for guided tissue regeneration and guided bone regeneration.

천공형 티타늄막을 이용한 골유도재생술 시 수종의 골이식재가 골재생에 미치는 영향

홍승범,권영혁,박준봉,허익,정종혁,Hong, Seung-Bum,Kwon, Young-Hyuk,Park, Joon-Bong,Herr, Yeek,Chung, Jong-Hyuk 대한치주과학회 2006 Journal of Periodontal & Implant Science Vol.36 No.1

The purpose of the present study was to evaluate the effect of bone graft materials including deproteinized bovine bone(DBB), demineralized freeze-dried bone(DFDB), freeze-dried bone(FDB) on bone formation in guided bone regeneration using perforated titanium membrane(TM). 16 adult male rabbits(mean BW 2kg) were used in this study and 4 rabbits allotted to each test group. Intramarrow penetration(diameter 6.5mm) was done with round carbide bur on calvaria to promote blood supply and clot formation in the wound area. The test groups were devided into 4 groups as follows: TM only(test 1), TM +DBB(test 2), TM +DFDB(test 3), TM +FDB(test 4). Perforated titanium membrane was contoured in rectangular parallelepiped shape(0.5mm pore diameter, 10mm in one side, 2mm in inner height), filled the each graft material and placed on the decorticated carvaria. Perforated titanium membrane was fixed with resorbable suture materials. The animals were sacrificed at 2, 8 weeks after the surgery. Non-decalcified preparations were routinely processed for histologic analysis. The results of this study were as follows: 1. Perforated titanium membrane was biocompatible. 2. Perforated titanium membrane had capability of maintaining the space during the healing period but invasion of soft tissue through the perforations of titanium membrane decreased the space available for bone formation. 3. In test 1 group without bone graft material, the amount of bone formation and bone maturation was better than other test groups. 4. Among the graft materials, the effect of freeze-dried bone on bone formation was best. 5. In the test groups using deproteinized bovine bone, demineralized freeze-dried bone, bone formation was a little. The spacemaking capability of the membrane may be crucial for bone formation. The combined treatment with the perforated titanium membrane and deproteinized bovine bone or demineralized freeze-dried bone failed to demonstrate any added effect in the bone formation. Minimization of size and numbers of perforations of titanium membrane or use of occlusive titanium membrane might be effective to acquire predictable results in the vertical bone formation.

다중화된 IP 트래픽의 자기유사성에 관한 연구

홍승범(Seung-Bum Hong),박재성(Jae-Sung Park),황주연(Ju-Yeon Hwang),이재용(Jai-Yong Lee) 한국통신학회 2000 한국통신학회 학술대회논문집 Vol.2000 No.11

강인한 특징 변수 선별과 신경망을 이용한 장면 전환점 검출 기법

홍승범(Seung Bum Hong),홍교영(Gyo Young Hong) 한국멀티미디어학회 2004 멀티미디어학회논문지 Vol.7 No.7

고성능 막 크로마토그래피에 의한 유청 단백질의 분리특성

홍승범(Seung Bum Hong),노경호(Kyung Ho Row) 한국생물공학회 2001 KSBB Journal Vol.16 No.6

양극성 히스토그램 기법을 이용한 샷 검출

홍승범(Hong seung-bum),권성은(Kwan sung eun),백종환(Baek joong-hwan) 한국통신학회 2002 한국통신학회 학술대회논문집 Vol.2002 No.7

형태적 특징 정보를 이용한 C.Elegans의 개체 분류

전미라,나원,홍승범,백중환,Jeon, Mi-Ra,Nah, Won,Hong, Seung-Bum,Baek, Joong-Hwan 한국통신학회 2003 韓國通信學會論文誌 Vol.28 No.7C

C.elegans are often used to study of function of gene, but it is difficult for human observation to distinguish the mutants of C.elegans. To solve this problem, the system, which can classify the mutant types automatically using the computer vision, is now studying. Tn previous work[1], we described the preprocessing method for automated-classification system. In this paper, we introduce shape features, which can be extracted from an acquisition image. We divide the feature into two categories, which are related to size and posture of the worm, and each feature is described mathematically We validate the shape information experimentally. And we use hierarchical clustering algorithm for classification. It reveals that 4 mutants of the worm, which are used in experiment, can be classified with over 90% of success rate. C.elegans 선충은 유전자 기능 연구에 주로 쓰이고 있으나, 변종들의 구분이 육안으로는 쉽지 않다. 이를 해결하기 위하여 컴퓨터 비젼을 이용하여 자동으로 분류할 수 있는 시스템이 연구 중이며, 이전 논문[1]에서 선충의 자동 분류 시스템에 사용될 영상의 전처리 과정에 대하여 서술한 바 있다. 본 논문에서는 전처리 된 영상 데이터를 이용하여 추출해 낼 수 있는 선충의 형태적 특징들을 제시한다. 선충의 크기와 관련한 특징과 자세에 관련한 특징으로 나누어, 각 특징의 추출 알고리즘을 수학적으로 표현하였다. 실험에서 제시된 형태적 특징 정보를 이용하여 직접 분류해 봄으로써 성능을 확인하였다. 분류 알고리즘은 Hierarchical Clustering을 사용하였다. 그 결과 실험에 이용된 선충의 4 종류 모두 90% 이상 옳게 분류되었다.

가와사키 병으로 인한 관상동맥 질환 소아의 무체외순환 관상동맥우회술의 마취 경험(증례보고)

홍성욱 ( Seong Wook Hong ),심재광 ( Jae Kwang Shim ),최용선 ( Yong Seon Choi ),홍승범 ( Seung Bum Hong ),곽영란 ( Young Lan Kwak ) 대한마취과학회 2008 Korean Journal of Anesthesiology Vol.54 No.4

Diamond™ Nucleic Acid Dye를 사용한 Touch DNA 시각화 및 검출

오대환(Oh, Dae Hwan),홍승범(Hong, Seung Bum),최하영(Choi, Ha Young),신강일(Shin, Kang Il) 경찰대학 경찰학연구편집위원회 2021 경찰학연구 Vol.21 No.3

본 연구에서는 범죄현장 및 증거물에 잠복되어 있는 DNA (Deoxyribose Nucleic Acid)를 시각화하여 정확한 위치에서 유전자 증거물을 채취하고자 젤 전기영동에 사용되는 핵산 염색 시약 중 하나인 Diamond™ Nucleic Acid Dye를 사용하였다. 사람이 손으로 만진 물품의 표면에 Diamond™ Nucleic Acid Dye(10,000X) 원액을 75% 에탄올로 20배 희석하여 염색하였다. 생물학적으로 근연관계가 없는 4명의 공여자에게 멸균 처리된 물품을 만지게 한 후, 20배 희석한 DD(Diamond™ Nucleic Acid Dye)를 물품의 표면에 피펫으로 점적하여 Touch DNA를 염색하였다. 그리고 Dino-Lite 디지털형광현미경을 사용하여 물품 표면의 형광을 확인하였다. DD 분자는 구조적으로 DNA의 골격(sugar-phosphate backbone)에 효과적으로 결합할 수 있기 때문에 ssDNA(single-stranded DNA), dsDNA(double-stranded DNA) 그리고 RNA를 형광으로 확인 할 수 있다. DD는 dsDNA를 기준으로 청색 파장(494nm)에서 최대 여기(excitation)를 가지며 DNA와 결합하면 녹색 형광(558nm)을 방출(emission)한다. 이로써 4명의 공여자가 만진 물품에서 녹색 형광을 방출하는 발광체를 관찰할 수 있었으며, 멸균 면봉에 멸균수를 묻혀서 녹색 형광을 발현하는 발광체를 채취하였다. 현재 경찰청에서 국립과학수사연구원으로 유전자 감정 의뢰과정과 동일하게 사용 중인 유전자 증거물 채취 키트에 포장하여 한국유전자정보연구원으로 가져간 후 STR(Short Tandem Repeat) profiling 분석을 하였으며, 공여자 4명의 구강상피세포 DNA에서 분석된 STR profile을 대조한 결과 개인식별이 가능한 것을 확인하였다. 이 방법이 보급 된다면 보이지 않는 DNA를 시각화하여 유전자증거물을 채취할 수 있도록 과학수사관을 안내하고 DNA가 없는 시료의 무분별한 감정 처리 시간 단축 및 예산 절약의 효과가 있다고 사료되었다. This study used Diamond™ Nucleic Acid Dye, a nucleic acid staining solution used for gel electrophoresis to precisely locate and collect genetic evidence from the crime scene and evidence by visualizing DNA (Deoxyribose Nucleic Acid). Diamond™ Nucleic Acid Dye (10,000X) was diluted by 20times with 75% ethanol and was used to dye the surface of the touched items. After having four biologically unrelated donors touch sterilized items, the diluted Diamond™ Nucleic Acid Dye(DD) solution was dripped onto the surface of the items using a pipette to stain the Touch DNA. A dino-Lite digital fluorescence microscope was then used to observe fluorescence. ssDNA(single-stranded DNA), dsDNA(double-stranded DNA) and RNA can be confirmed with fluorescence since the structure of DD molecules effectively combine DD molecules with the sugar-phosphate backbone of the DNA. With dsDNA at the baseline, DD has the greatest excitation at the blue wavelength(494nm) and emits green fluorescence(558nm) when combined with DNA. Green fluorescence was observed on the items touched by the four donors, and sterilized cotton swabs moistened with sterile water were used to collect luminous matter emitting green fluorescence. To replicate the current genetic analysis request procedure, the sample was placed in the genetic evidence collection kit, sent to the Korea Genome Information Institute from the police agency, and analyzed with STR(Short Tandem Repeat) profiling. A comparison with the STR profiles derived from the buccal epithelial cells of the four donors confirmed that the collected sample could successfully identify the donors. Widespread use of the technique presented in this research is expected to visualize invisible DNA and guide forensic field agents, reduce the processing time of DNA-less staining solution and result in a budget-saving.