The Effect of Methanol Extract of Taxillus Yadoriki (Siebold) Dancer on MC3 and YD15 Mucoepidermoid Carcinoma Cells

한혜연,장민아,유미현 대한구강악안면병리학회 2023 대한구강악안면병리학회지 Vol.47 No.2

Taxillus yadoriki (Siebold) Dancer is a parasitic plant that grows on camellia trees and is common on Jeju Island. The branches of T. yadoriki have long been used to treat various diseases, including hypertension, diabetes mellitus, viral infections, and arthritis. Although recent studies reported that T. yadoriki has anticancer effects in various human cancer cell lines, including lung cancer, the exact molecular mechanisms supporting its anticancer effects are not well understood. This study aims to assess the anticancer effect of the methanol extract of T. yadoriki branches (METY) on mucoepidermoid carcinoma (MEC) cell lines (MC3 cells and YD15 cells) and explore its mechanism of action. Inhibitory activity of MEC cell proliferation was assessed using the CCK-8 assay. The mechanism of the anticancer effect on METY-treated MC3 cells and YD15 cells was evaluated with Hoechst 33342 stain and Western blot. After treating MC3 cells and YD15 cells with METY for 48 hours, the cytotoxicity of MC3 and YD15 cells increased, and nuclear fragmentation increased in both METY-treated MEC cells. Caspase-3 and cleaved PARP activation demonstrated apoptosis of METY-treated MEC cells. Cell proliferation inhibition with METY was alleviated in METY-treated MEC cells pretreated with zVAD-FMK, supporting the cell proliferation inhibition effect by apoptosis. METY-induced apoptosis in MEC cells occurs through MAP kinase pathways such as p38 and pAkt. MEC cell. METY-induced apoptosis of MEC cells occurs via the p38 and pAkt MAPK pathways. Therefore, METY may be a promising anticancer candidate for the MEC therapeutic strategy.

전문도서관 Best Practice - LG상남도서관 영어논문 지원 서비스

한혜연,Han, Hye-Yeon 한국전문도서관협의회 2011 KSLA bulletin Vol.3 No.-

과학기술이 현격하게 변화한 것만큼 웹 공간에서의 과학기술 정보시스템 및 과학기술 연구자들의 정보이용 행태도 빠르게 변화하여 왔다. LG상남도서관의 LG ELIT(http://www.lg.or.kr)는 과학기술분야 연구자들을 위한 과학기술 정보서비스를 제공하면서 과학기술 연구자가 원하는 차별화된 서비스를 제공하고자 노력해왔다. 이 사례 소개에서는 영어논문 튜토리얼 및 영어논문 감수 서비스에 대한 설명과 더불어 서비스 준비 과정을 공유함으로써 새로운 서비스를 준비하는 분들에게 도움을 주고자 한다.

The Expression of NF-κB p65 Protein in Oral Squamous Cell Carcinoma from Korean Oral Cancer Patients

한혜연,박원익,정성희,장혜옥,배수경,유미현 대한구강악안면병리학회 2014 대한구강악안면병리학회지 Vol.38 No.6

Oral squamous cell carcinoma (OSCC), is the most common malignancy of oral cavity, and the sixth most frequently diagnosedcancer worldwide. This tumor type is associated with poor prognosis, and most OSCC patients are diagnosed after the cancer hasreached an advanced stage. The over expression of NF-κB p65 has been associated with OSCC progression and lymph node metastasis. Hence, the present study analyzed the expression of NF-κB p65 in OSCC from Korean patients. Immunohistochemistry for NF-κBp65 was performed using 12 normal oral mucosas (NOM), 16 oral leukoplakia (with/without dysplasia), and 58 OSCC patients samples. Immunoreactivity was semi-quantitatively scored and the correlation between the expression of NF-κB p65 and clinicopathologicalparameters of OSCC patients was analyzed. Immunohistochemical staining revealed that NF-κB p65 expression level increased in oralleukoplakia with dysplasia and OSCC. Moreover, the immunoexpression of NF-κB p65 appeared to be associated with age, recurrence,TNM stage, and lymph node metastasis in OSCC patients (p<0.05). These results indicated that NF-κB p65 can play a role as oncogenein OSCC. Moreover, NF-κB p65 may play an important role in both oral carcinogenesis and OSCC patient outcome. It may be consideredas another new malignant biomarker of OSCC.

뇌졸중 환자의 환측상지에 동반된 말초신경손상에 대한 연구

한혜연,안경회 大韓再活醫學會 1989 Annals of Rehabilitation Medicine Vol.13 No.2

The Extract of Kochia scoparia Fruit Induces Programmed Necrosis in Oral Squamous Cell Carcinoma Cells

한혜연,박봉수,김형준,정승화,김지연,정성희,김규천,황대석,김욱규,김형우,유미현 대한구강악안면병리학회 2016 대한구강악안면병리학회지 Vol.40 No.6

The fruit of Kochia scoparia Scharder is traditionally used as a medicinal ingredient to treat allergic skin diseases and inflammatory diseases in China, Japan and Korea. Recently, several studies reported that K. scoparia had potential for the cytotoxicity of human cancer cells. To investigate the anti-cancer effect of K. scoparia on oral cancer and to determine the specific type of cell death induced by MEKS treatment. We investigated the anti-cancer effects of K. scoparia, methanol extract (MEKS) in HSC4 human oral cancer cells. We examined the effects of MEKS on the proliferation rate, cell cycle arrest, 7-AAD-ANNEXIN V double stain, reactive oxygen species (ROS) generation and activation of apoptosis and necroptosis-associated proteins in HSC4 cells. MTT assay results demonstrated that MEKS decreased the proliferation rates of HSC4 cells in a dose-dependent manner with an IC50 value of 45.3 μg/ml. MEKS at 50 μg/ml significantly increased the sub-G1 DNA contents of HSC4 cells to 84.8%, versus untreated cells. However, the activation of apoptosis-associated proteins such as cleaved caspase 3, cleaved caspase 8, cleaved caspase 9 and cleaved Poly (ADP-ribose) polymerase (PARP) did not detect. The level of Bax protein markedly increased in MEKS-treated HSC4 cells. In addition, the cell viability of the DPQ pre-treated HSC4 cells with MEKS treatment was significantly greater than that of MEKS treated-cells. These results suggest that MEKS inhibits cell proliferation and induces necroptosis in oral cancer cells and that MEKS may have potential chemotherapeutic value for the treatment of human oral cancer.

이웃사촌 만들기 프로젝트

한혜연 샘터사 2020 월간 샘터 Vol.599 No.-

LG상남도서관

한혜연,이혜리 (사)한국인터넷전문가협회 2017 Web Korea Annual Vol.2017 No.-

어린이의 전시 관람 좌정에 관한 인류학적 연구

한혜연 청운대학교 방송예술연구소 2014 미디어와 공연예술연구 Vol.9 No.2

이 연구는 어린이들의 전시 관람을 통한 문화예술의 경험을 관람의 목적과 목표, 선택의 과정, 경험이 갖는 의미를 중심으로 조사, 분석한 것이다. 연구를 위해 설문, 참여관찰 과 인터뷰, 인터넷 행태, 정책과 문헌에 대한 자료조사를 통해 인류학적으로 고찰함으로써 예 술경험과 문화산업의 관계를 규명하고자 하였다. 미래 인재 양성의 방향을 창의성과 문화 분야로 상정하고 있는 한국의 교육적 담론과 정책 속에서 어린이의 예술 경험은 강조되고 있다. 그러나 그 경험의 주체는 어린이가 아니며, 경험 의 과정은 제도 교육과 문화산업, 예술 시장이 만드는 산업의 맥락에 위치한다. 특히 상업화된 전시의 경험 과정이 주최측의 광고와 마케팅의 영향 속에서 부모와 학교에 의해 선택된다는 점, 상업적 매커니즘에 의해 만들어진 콘텐츠에 노출된다는 점은 어린이들이 이윤을 목적으로 하는 문화산업에 편입되고 있음을 보여준다. 또한, 전시 관람의 목적과 효과, 내용에 대한 방향 이 교과 교육에 대한 도움과 주류사회의 문화 체험에 있다는 점은 기존 제도 교육의 틀을 벗어 나지 못한 한계를 보여 준다. 예술이 갖는 긍정적인 영향력과 효과는 어린이에게 중요한 경험이다. 따라서 날로 광폭해 져 가는 문화산업의 폐해에 맞서는 대안이 되기 위해 전시 관람과 예술 경험의 과정을 재고해 야 한다. This study researches goals and objectives, choosing process, experience contents of children's viewing exhibitions and experiencing art. This anthropological study analyzes the relationship between children's experience of art and the cultural industry through participatory observation, interview, policy and literature review, internet search, etc. Children's experience of art has been the focus of national educational discourses and policies, which emphasize on creativity and culture for cultivating young talents. However, children fail to independently experience art. Their experience significantly depends on educational system, cultural industry, and art market. Especially, their experiences of commercial exhibitions are often chosen by their parents or schools that are strongly influenced by sponsors' advertisements and marketing strategies. Therefore children are easily exposed to the contents produced by the commercial mechanism, and their experience can be reduced to a part of the cultural industry pursuing profit. In addition, the goals, contents, and effects of children's experience of art are closely related to the school curriculum and the mainstream culture. Therefore most of these experiences are within the limitations of the existing educational system. Art has positive effects on children's experience. The experience of art should be considered as an alternative to the services of the cultural industry, which is increasingly provocative and violent only pursuing profit.

치주염 조직에서 발현이 증가하는 CTGF에 의한 파골세포 생존 증가

한혜연,박종철,유미현,배문경,김형준 대한구강악안면병리학회 2017 대한구강악안면병리학회지 Vol.41 No.4

Connective tissue growth factor (CTGF, CCN2) is one of the multi-functional secreted proteins which belong to CCN family of cysteine-rich growth factors. CTGF is known to have pivotal roles in embryonic endochondral ossification but its role in relevance to periodontitis is never been determined. To identify new molecular mediators associated with periodontitis-induced bone resorption, we have analyzed publicly available GEO database and found the markedly augmented CTGF mRNA expression in periodontitis gingival tissues. The existence of CTGF significantly enhanced mature osteoclasts survival which accompanied by reduction in TUNEL-positive nuclei and PARP cleavage. These results may provide another line of evidence the CTGF mediated prolonged osteoclast survival and subsequent increased bone resorption in the periodontitis patients.

아동의 장애유형·장애정도와 어머니의 욕구요인과의 관계(요약)

한혜연 강남사회복지대학 특수교육학과 1988 특수교육 Vol.1 No.-