낮은 농도의 Hypoxanthine과 FSH가 미성숙난자의 체외성숙에 미치는 영향

한혁동,임창교,염현식,현나영,이지향,홍미,Han, Hyuck-Dong,Lim, Chang-Kyo,Youm, Hyun-Sik,Hyon, Naomi Na-Hyoung,Lee, Ji-Hyang,Hong, Me 대한생식의학회 2009 Clinical and Experimental Reproductive Medicine Vol.36 No.3

목 적: Hypoxanthine (Hx)과 FSH가 미성숙난자의 배양에 미치는 영향을 관찰하기 위해 미성숙난자를 배양하여 GVBD, MII기 발생률을 비교 관찰하였다. 연구방법: 단순배양액인 BSAL-XI-HTF 배양액을 사용하여 (1) 0.3% BSA mBASAL-XI-HTF (0.3% BSA 배양액), (2) 0.1 IU/ml FSH를 첨가한 0.3% BSA mBASAL-XI-HTF (FSH 0.3% BSA 배양액), (3) 10% FBS mBASAL-XI-HTF (10% FBS 배양액), (4) 0.1 IU/ml FSH를 첨가한 10% FBS mBASAL-XI-HTF (FSH 10% FBS 배양액)의 4종류의 배양액을 만들었고, 각 종류의 배양액에서 생쥐 난구세포로 둘러싸인 미성숙난자의 성숙을 3, 6, 18시간별로 비교 관찰하였다. 각 배양액에 1 mM, 2 mM, 4 mM 농도의 미성숙난자성숙억제제인 Hx을 섞어 난자의 성숙억제양상을 관찰하였고 GVBD와 MII기 발생률을 비교 관찰하였다. 결 과: Hx을 첨가하지 않은 4종류의 배양액에서 미성숙난자의 자연성숙은 3시간 내에 대부분 GVBD가 발생하였고 MII기로의 발육은 6시간 이후 발생하였다. 18시간 후 각 군의 배양에서 모두 유사한 GVBD 발생률을 보였다. 1 mM, 2 mM, 4 mM Hx 농도의 0.3% BSA 배양액과 10% FBS 배양액에서 난자의 성숙억제양상을 비교해 보면 4 mM 농도의 Hx 배양액에서 18시간 동안 완전한 성숙억제를 보였다. 2 mM 농도의 Hx 배양액에서도 18시간 배양까지 억제를 보였으나 4 mM 농도의 Hx 배양액보다 작은 난자성숙억제양상을 보였다. 1 mM 농도의 Hx 배양액에서는 모두 난자성숙억제를 보이지 못했다. FSH를 첨가한 배양액에서는 2 mM 농도의 Hx 뿐만 아니라 1 mM 농도의 Hx 첨가에서도 초기 3시간까지 GVBD 성숙이 억제되었다. 또 같은 농도의 Hx을 함유하고 FSH를 첨가하지 않은 배양액에 비해 FSH를 첨가한 배양액에서 3시간, 6시간 동안 GVBD 발생을 더 억제하였다. 그러나 18시간 배양 후 난자성숙이 회복됨을 보였다. 이 결과로 FSH가 배양초기에 난자성숙을 억제하나 후기에는 성숙을 촉진함을 알 수 있었다. 18시간 후 MII기발육은 4 mM 농도의 Hx을 함유한 모든 배양액에서 낮은 발생률을 보였고, FSH를 함유한 10% FBS 배양액에 1 mM, 2 mM의 낮은 농도의 Hx을 첨가한 배양액은 다른 배양액에 비해 통계학적으로 높은 MII기 발생률을 보였다. 결 론: 본 실험에서 낮은 농도의 Hx과 FSH는 FBS를 함유한 배양액에서 미성숙난자의 초기배양 동안 성숙을 억제한 후 MII기 성숙을 촉진함을 알 수 있었다. Objective: We examined the effect of different culture media on oocyte maturation. Methods: Four groups of media, (1) 0.3% BSA mBASAL-XI-HTF, (2) 0.3% BSA mBASAL-XI-HTF with FSH, (3) 10% FBS mBASAL-XI-HTF and (4) 10% FBS mBASAL-XI-HTF with FSH were prepared. Mouse cumulus enclosed oocytes (CEOs) were incubated in each group of medium. Hypoxanthine (Hx) was mixed to each group of medium in increasing concentrations of 1 mM, 2 mM and 4 mM. CEOs were incubated and assessed for GVBD and MII development at 3, 6, 18 hours. Results: CEOs maturation to GVBD was seen in all four groups during 3 hours of culture, however MII stage of oocytes was seen after 6 hours. Complete arrest of GV stage in 4 mM Hx media without FSH and partial arrest in 2 mM Hx media without FSH were seen during 18 hours of culture but development was not suppressed in 1 mM Hx media without FSH. More prominent GVBD suppression was noted at early 3, 6 hours culture in 1 mM, 2 mM Hx media with FSH compared to media without FSH. But the suppression was recovered at 18 hours. This result suggests that low concentrated Hx and FSH supplemented media can suppress CEOs maturation during early culture period but recovery is resumed or even stimulated at late period. 1 mM, 2 mM Hx 10% FBS medium with FSH had significantly higher rates of MII development (71.7%, 66.7%) at 18 hours compared to other media. Conclusion: Our results show that low concentrated Hx and FSH supplemented 10% FBS media may stimulate MII development after an initial inhibitory effect.

생쥐초기배아 세포기가 동결보존 및 융해후 생존에 미치는 영향

한혁동,김영대,손성욱,권장연,이영진,정인배,차동수,Han, Hyuck-Dong,Kim, Young-Dae,Shon, Sung-Wook,Kwon, Jang-Yeon,Lee, Young-Jin,Chung, In-Bae,Cha, Dong-Soo 대한생식의학회 1993 Clinical and Experimental Reproductive Medicine Vol.20 No.2

To forsee appropriate developmental cell stage in human embryos for cryopreservation, we observed blastocyst development in culture medium after cryopreservatlon and thawing of one cell, two cell, four cell stage of mice embryos. According to our results, development of the blastocyst of cryopreserved two cell mice embryos was significantly higher than that of cryopreserved one cell mice zygotes or four cell mice embryos.

생쥐 1-세포기 수정란의 동결방법에 있어서 초자화동결과 완만동결의 비교

이지향,한혁동,구혜영,Lee, Ji-Hyang,Han, Hyuck-Dong,Koo, Hye-Young 대한생식의학회 2001 Clinical and Experimental Reproductive Medicine Vol.28 No.3

Objective : This study was conducted to examine the effect of vitrification on the survival and in vitro development of mice 1-cell zygotes. Method: Effects of exposure to vitrification solution and vitrification, with different concentrations of the cryoprotectant solution, were examined. The 1-cell zygotes were also subjected to a slow freezing-thawing method to compare with vitrification method. Solution composed of ethylene glycol (6.0 M, 5.0 M, 4.0 M) and sucrose (1.0 M) were used as cryopropectant. The experiments employed the method loading the embryos on electron microscope grids. Results: I. The effects of exposure in vitrification solution. 1-cell zygotes were non-toxic at all concentrations of the vitrification solution showing the survival rate between 88.1% and 97.5%. Development into 2-cell was more successful in the higher concentrations of the vitrification solution. Therefore, higher concentrations of the vitirification solution do not seem to cause any problems in vitrification procedure. II. The effects of vitrification method. 1-cell zygotes showed the survival rate between 78.8% and 92.4%. The lowest and the highest survival rate was observed in the 6.0 M and 4.0 M vitrification solution, respectively. 2-cell development rates varied from 77.6% to 91.3%. Blastocyst development rate was shown highest in 5.0 M and the lowest in 4.0 M solution. Therefore, the highest 2-cell and blastocyst development rate was observed in 5.0 M solution. III. Comparison of vitrification and slow freezing-thawing method on 1-cell zygotes. This experiment showed that 1-cell zygotes had the highest survival and development rates in 5.0 M vitrification solution. Vitrified group of 1-cell zygotes, in the 5.0 M vitrification solution, were compared with the group processed in slow freezing-thawing method. The development rate into 2-cell and blastocyst as well as the survival rate were higher in the vitrified group than in the slowly freezed group. Conclusion: 1. The results demonstrate that the best cryoprotectant is a 5.0 M vitrification solution for 1-cell zygotes. 2. Vitrification method significantly increases the survival rate of the 1-cell zygote and its development into 2-cell and blastocyst. Equilibration and exposure time during the vitrification was remarkerbly short in this experiment. Total time, from the exposure to vitirification solution to storage in the liquid nitrogen, was taken only 90 seconds. In contrast, the slow freezing-thawing method have taken more than four hours. Taken together, we presume that the overall time used for the procedure contributes to the results as an important parameter. 3. The loading of 1-cell zygotes on the EM grid is technically more simple and takes less time than the straw or cryo vial method.

체외수정시술을 위한 과배란유도시 Highly Purified FSH 사용 군과 FSH / hMG 사용 군과의 비교연구

최기동(Ki Dong Choi),홍민(Min Hong),김일한(Ill Han Kim),최창익(Chang Ik Choi),오갑영(Gab Young Oh),한혁동(Hyuck Dong Han) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.8

불임환자에서 체외수정을 위한 과배란 유도시 사용되어지는 FSH 제재로는 0.01 IU의 LH를 함유하고 있는 FSH와 약0.006 IU의 LH를 함유하고 있는 Highly Purified FSH(FSH-HP)등이있다. 불임환자의 체외수정을 위한 과배란 유도시 FSH 제재만으로도 정상적인 난포의 성장 및 임신이 가능하며 또한 많은 양의 LH는 임신율을 감소 시키는 것으로 알려져왔다. 따라서 본연구에서는 FSH-HP만 사용한 군과 FSH/hMG를 사용한군 사이의 체외수정을 위한 과배란 유도결과를 비교 분석하기 위하여 시행하였다. 본 연구는 체외수정을 위한 과배란 유도 총 280주기중 FSH-HP사용군이 111주기이고, FSH/hMG사용군이 169주기였다. 각 연구군에서 불임환자의 연령, 투여된 Gonadotropin의 양 및 기간,이식한 배아의 수, 자궁내막의 두께, 수정율 등은 유의한 차이가 없었다. 채취된 난자의 수는 두 투여군 사이에 유의한 차이가 없는 반면 hCG 투여일의 혈중 E2 농도는FSH/hMG 투여군에 비하여 FSH-HP투여군에서 유의하게 낮았다. 배아의 질면에서는 GradeⅠ,Ⅲ,Ⅳ 의 배아에서는 두군간차이는 없었으나 GradeⅡ 의 경우 0.22±0.67개와 0.06±0.24개로 FSH-HP 투여군이 유의하게 높았으며 배아의 GradeⅠ 과Ⅱ를 합한 경우 FSH-HP투여군이 2.56±1.78, FSH/hMG투여군이2.06±1.73으로 FSH-HP투여군이 유의하게 높았다. 임신율에 있어서는 38.8% Vs 34.2% 로 FSH-HP 투여군이 높은경향을 보였으나 유의한 차이는 없었다. 이와같은 결과로 볼 때 FSH-HP 사용이 FSH/hMG 사용보다 더 양질의 배아를 얻을수 있었고 FSH-HP 투여군이 임신율에 있어서도 유의한 차이는 아니지만 높은 경향을 보였으므로 효과적인 과배란 유도의 한 방법으로 사용되어질수 있을것으로 생각되어진다. Objective : It is known that normal follicular growth and pregnancy are possible using Follicular stimulating hormon(FSH) alone during controlled ovarian hyperstimulation(COH) for IVF-ET. The purpose of this study was to compare and analize the results of COH for IVF-ET between a group using only FSH-HP and a group using FSH/hMG Method : 111 cycle of the total 280 COH for IVF-ET cycles belonged to the FSH-HP group, and the FSH/hMG group accounted for remaining 169 cycles. The amount of gonadotropin administered, the duration of hormonal therapy, the number of embryos transfered, the endometrial thickness, and the pregnancy rate were compared between the two groups and statistically analized using the t-test. p values less than 0.05 deemed statistically significant. Result : There were no statistical differences on the amount of gonadotropin administered, the duration of hormone therapy, number of oocytes retrieved, nor the endometrial thickness between the two groups. However,the serum E2 level was significantly lower, and the number of embryos of high quality(GradeⅠandⅡ) significantly higher in the FSH-HP group. In pregnancy rate, FSH-HP group shows higher than FSH/hMG group(38.8% VS 34.2%). but the difference was not large enough to be considered statistically significant. Conclusion : The results of this study show that compared to using FSH/hMG, treatment with FSH-HP alone allowed the retrieval of more embryos of high quality, and although not statistically significant, achieved a higher pregnancy rate, thus indicating that it can be used as an effective method for COH.

태아 성별에 따른 태아 대뇌동맥 도플러 파형의 변화

최성진 ( Seong Jin Choi ),차동수 ( Dong Soo Cha ),한혁동 ( Hyuck Dong Han ),이영진 ( Young Jin Lee ),한경희 ( Kyoung Hee Han ),정인배 ( In Bai Chung ) 대한산부인과학회 2005 Obstetrics & Gynecology Science Vol.48 No.10

자궁근종의 경피적 혈관색전술 후 경험한 자궁근종괴사 1 예

홍민(Min Hong),김일한(Ill Han Kim),한혁동(Hyuck Dong Han),김영주(Young Ju Kim),송지선(Ji Sun Song),조미연(Mee Yon Cho) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.2

Uterine fibroids are the most common benign tumor of the female genital tract. Traditional therapy for symptomatic fibroids has been either myomectomy or hysterectony, depending on whether futhur fertility is desire. A promised new altermative therapy-embolization of the uterine arteries- is now available. A 29 year-old woman was diagnosed of uterine fibroid by ultrasonogram and other techniques. We have experienced one case of uterine fibroid necrosis after transarterial embolization. Now we report a case of uterine artery embolization for treattement of uterine fibriods with a brief review.

자궁동맥 색전술 후 자궁근종의 질 탈출 1 예

고양순(Yang Soon Ko),홍민(Min Hong),차동수(Dong Soo Cha),한혁동(Hyuck Dong Han),이영심(Young Sim Lee),김영주(Young Ju Kim),송지선(Ji Sun Song),정순희(Soon Hee Jung) 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.10

Uterine myomas are the most common benign tumor of the female genitalia tract. Traditional therapy for symptomatic fibroids has been either myomectomy or hysterectomy, depending on whether further fertility is desired. Myomectomy is a surgical alternative, it is associated with greater morbidity and a higher blood transfusion rate. Arterial embolization of myomas is non surgical treatment option, is cheaper than myomectomy, permits preservation of reproductive potential, may be associated with less morbidity than myomectomy, and may not cause adhesions which could compromise fertility. A promised new alternative therapy-embolization of the uterine arteries is now available. A 26 year-old woman was diagnosed as submucosal uterine fibroid by ultrasonogram. We have recognized uterine arterial embolization is an effective therapy in the management of symptomatic myomas. Now we report vaginal passing of uterine fibroid after uterine arterial embolization.

임신중 요로감염증의 선별검사에 관한 비교연구

권장연 ( Jang Yeon Kwon ),이영진 ( Young Jin Lee ),차동수 ( Dong Soo Cha ),한혁동 ( Hyuck Dong Han ),한상원 ( Sang Won Han ),정인배 ( In Bai Chung ),김대현 ( Dae Hyun Kim ),어영 ( Uh Young ) 대한주산의학회 1993 Perinatology Vol.4 No.2

선천성기형에 대한 임상적 고찰

김병성 ( Byung Sung Kim ),정인배 ( In Bai Chung ),권장연 ( Jang Yeon Kwon ),이성림 ( Sung Lim Lee ),한혁동 ( Hyuck Dong Han ),차동수 ( Dong Soo Cha ) 대한주산의학회 1994 大韓周産醫學會雜誌 Vol.5 No.4

임신과 동반된 난소종양의 임상적 고찰

장정호 ( Jeong Ho Jang ),최현일 ( Hyun Il Choi ),이흥순 ( Heung Soon Lee ),황명중 ( Myung Joon Hwang ),한혁동 ( Hyuck Dong Han ),차동수 ( Dong Soo Cha ),이영진 ( Young Jin Lee ) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.11

목적: 임신과 동반된 난소종양에 대한 임상양상을 평가하기 위함이다. 연구 방법: 1995년 1월부터 2000년 12월까지 본원 산부인과에 산전관리 및 분만을 위해 내원한 임산부 중 수술적 요법을 시행받고 조직병리 검사상 난소종양으로 확진된 51예를 분석하였다. 외래 기록지, 수술 기록지, 분만 기록지, 병리 소견서 등을 통하여 임상양상 및 임신에 미치는 영향을 연구하였다. 결과: 1. 발생빈도는 조사기간 동안 총 분만건수 11,056예 중 51예로 0. Objective: We performed this study to evaluate the clinical appearance of ovarian timor in pregnancy. Materials and Methods: From January 1995 to December 2000, a review was performed 51 cases of ovarian tumors in pregnancy. The diagnosis of ovarian tumor