Suspected Unilateral Renal Dysplasia in a Cat with Intermittent Hematuria

한재익,나기정 한국임상수의학회 2015 한국임상수의학회지 Vol.32 No.1

A 6-month-old, castrated male, Korean short-haired cat was referred with intermittent hematuria. Physical and laboratory examination including complete blood count and serum biochemistry profiles showed no specific findings. Ultrasonography revealed a cystic lesion of the mid-abdomen, which was approximately 2 × 2 cm in size. FNA cytology of cystic fluid showed numerous cholesterol crystals of varying size and shape and granulomatous inflammation. CT examination showed fluid-filled large cystic lesion in the left side of the abdomen. Taken together, this case was diagnosed as unilateral (left) renal dysplasia and cystic inflammation led by cholesterol crystallization in the left kidney.

닥스훈트 개의 특발성 다발성 결절 지방층염 증례

한재익,장동우,김근형,나기정 한국임상수의학회 2008 한국임상수의학회지 Vol.25 No.5

A 2-year-old intact female dachshund dog was presented with recurrent subcutaneous nodules and fistulations on the neck, back and hip. The patient was diagnosed as a sterile nodular panniculitis based on the cytology, histopathology, and cultures for bacteria and fungus. The fistulas were surgically removed and methylprednisolone was administrated 2 mg/kg twice daily per oral. The lesions were all disappeared, but the relapses were happened when the dosage of the drug was tapered off. The repeated treatments with methylprednisolone or azathioprine were performed, consequently, the patient was controlled with relatively low-dosage methylprednisolone (0.25 mg/kg, every third day).

Multiplex Quantitative Real-time Polymerase Chain Reaction Assay for Rapid Detection of Mycobacterium avium subsp. paratuberculosis in Fecal Samples

한재익,정영훈,최창용,유재규,강석진,유한상,박흥태,권응기,조용일 한국임상수의학회 2015 한국임상수의학회지 Vol.32 No.3

Mycobacterium avium subsp. paratuberculosis (MAP) causes paratuberculosis or Johne's disease, an intestinal granulomatous infection in domestic and wild animals. The study aimed to develop and evaluate a panel of multiplex quantitative real-time polymerase chain reaction (mqPCR) assay for simultaneous detection of three MAP-specific genes (IS900, F57 and ISMAP02 genes). The analytic sensitivity (i.e., limit of detection, expressed as cells per 1 ml) was 150 for IS900, 1500 for F57, and 50 for ISMAP02. The specificity of the method was determined by testing 152 bovine fecal samples. Based on the test, it showed that the assay simultaneously detected the target genes in short period of time and at lower cost compared to laboratory routine tests. The test agreement between the assay and routine test was 94%. The discrepancy in the results was due to samples that were tested positive by the panel but negative by the routine tests, suggesting that the assay has higher sensitivity than the routine tests. In conclusion, the mqPCR assay could be a rapid and accurate testing tool for investigating paratuberculosis or Johne's disease cases in domestic and wild animals.

Pulmonary Candidiasis in a Dog Receiving Long-term Management for Atopic Dermatitis

한재익,이창민,김태우,이인,최수영,박희명 한국임상수의학회 2015 한국임상수의학회지 Vol.32 No.3

A canine patient exhibited partial anorexia and sudden respiratory distress. Diagnostic imaging and cytology of tracheal-lavage fluids revealed fungal pneumonia. Molecular identification and phylogenetic analysis detected Candida catenulata. Treatment with oral itraconazole for 3 weeks was effective. This is the first report of C. catenulata infection in a dog.

Molecular Detection of Haemoproteus in Two Wild Eurasian Eagle Owls (Bubo bubo) in Middle Area of South Korea

한재익,장혜진,이종원,강효민,나기정 한국임상수의학회 2015 한국임상수의학회지 Vol.32 No.3

This report describes asymptomatic Haemoproteus infection in wild Eurasian eagle owls (Bubo bubo) diagnosed by blood smear and DNA analysis. This is the first description of natural Haemoproteus infection in wild Eurasian eagle owl in South Korea and suggests that the wild resident bird population can be a source for Haemoproteus infection of domestic poultry.

Bacterial Flora of the Intestine in Normal Captive Oriental White Storks

한재익,장혜진,이숙진,강효민,김수경,박시룡,나기정 한국임상수의학회 2011 한국임상수의학회지 Vol.28 No.5

A survey was conducted to examine the normal intestinal bacterial flora of captive Oriental white storks (Ciconia boyciana) maintained at the Korea Institute of Oriental White Stork Rehabilitation Research, Cheongwon,South Korea. From the cloaca of 44 healthy storks, 44 fecal samples were collected and cultured under aerobic and anaerobic conditions. The 16S ribosomal RNA gene sequences and the heat shock protein 60 gene were cloned and sequenced for bacterial identification. Under aerobic conditions, Enterococcus faecalis, Escherichia coli, Bacillus spp., Enterococcus avium, Enterococcus gallinarum, Pseudomonas spp., Alcaligenes spp., Enterobacter spp., Corynebacterium spp., and Proteus mirabilis were identified. Under anaerobic conditions, E. coli, Clostridium tertium, En. faecalis, and P. mirabilis were identified. E. coli, En. faecalis, or both were isolated from all samples. These results will add to the information available on this stork species and help for the interpretation of fecal culture results.

A multiplex quantitative real-time polymerase chain reaction panel for detecting neurologic pathogens in dogs with meningoencephalitis

한재익,장동우,나기정 대한수의학회 2015 Journal of Veterinary Science Vol.16 No.3

Meningoencephalitis (ME) is a common inflammatory disorder of the central nervous system in dogs. Clinically, ME has both infectious andnon-infectious causes. In the present study, a multiplex quantitative real-time polymerase chain reaction (mqPCR) panel was optimized forthe detection of eight canine neurologic pathogens (Blastomyces dermatitidis, Cryptococcus spp., Neospora caninum, Borrelia burgdorferi,Bartonella spp., Toxoplasma gondii, Ehrlichia canis, and canine distemper virus [CDV]). The mqPCR panel was subsequently applied to 53cerebrospinal fluid (CSF) samples collected from dogs with ME. The analytic sensitivity (i.e., limit of detection, expressed as molecules per1 L of recombinant vector) was 3.8 for CDV, 3.7 for Ehrlichia canis, 3.7 for Bartonella spp., 3.8 for Borrelia burgdorferi, 3.7 for Blastomycesdermatitidis, 3.7 for Cryptococcus spp., 38 for Neospora caninum, and 3.7 for Toxoplasma gondii. Among the tested CSF samples, seven (15%)were positive for the following pathogens in decreasing order of frequency: Cryptococcus spp. (3/7), Blastomyces dermatitidis (2/7), andBorrelia burgdorferi (2/7). In summary, use of an mqPCR panel with high analytic sensitivity as an initial screen for infectious agents in dogswith ME could facilitate the selection of early treatment strategies and improve outcomes.

Gizzard Impaction with Bile Stasis in Captive Oriental White Storks (Ciconia boyciana)

한재익,손형원,모인필,장동우,김수경,박시룡,나기정 한국임상수의학회 2009 한국임상수의학회지 Vol.26 No.6

The Korea Institute of Oriental White Stork Rehabilitation Research (KIOWSRR) is breeding oriental white storks for residential rehabilitation in South Korea. Three oriental white storks were referred with anorexia and depression. Two storks were died before examinations and one stork was died after 1 day of fluid therapy. On necropsy, salmon-colored subcutaneous and visceral fats were examined in all storks. Multiple focal necroses on liver surface were also examined. In gizzards, many gravels and grasses were severely tangled; therefore, it produced a large mass that blocked digestive system of the storks. Small intestinal segments of the stork, which was dead after the fluid therapy, were severely adhesive. On histopathology, diffuse necroses with/without inflammation were examined in the liver, gizzard and intestine in all storks. Bile stasis was additionally examined in the liver. According to the results of the examinations, all storks were diagnosed as gizzard impaction caused by parorexia.

Extreme Leukemoid Reaction in a Dog with Pyometra

한재익,장혜진,전귀호,강현구,나기정 한국임상수의학회 2009 한국임상수의학회지 Vol.26 No.6

Leukemoid reaction has been rarely encountered in dogs. This report describes a case of leukemoid reaction showing extremely elevated leukocyte count. A 10-year-old female Maltese dog was presented with purulent and bloody vaginal discharge. The CBC revealed severe leukemoid reaction (976 × 109 cells/l, reference range 6 to 17 × 109 cells/ l) and mild non-regenerative anemia. On the blood smear, elevated leukocytes consist of numerous neutrophils and monocytes only. Radiographic and ultrasonographic examinations revealed generalized enlargement of the uterus, which was filled with a complex fluid. After ovariohysterectomy, Escherichia coli was isolated from the uterine fluid. To author’s knowledge, it is the new record of extreme leukemoid reaction caused by local bacterial infection in dogs.

Community-acquired Extended-spectrum and Plasmid-mediated ampC Beta-lactamase-producing Multidrug-resistant Enterobacter cloacae Septicaemia in a Cat with Euthyroid Sick Syndrome

한재익,나기정 한국임상수의학회 2015 한국임상수의학회지 Vol.32 No.2

A 7-year-old castrated male Korean Shorthair cat was referred with lethargy and anorexia. Laboratory examination revealed moderate degenerative changes of peripheral neutrophils on blood smear examination and decreased levels of free and total thyroxine (T4) as well as bacterial growth on blood culture. Molecular analyses of the 16S ribosomal RNA gene and heat shock protein 60 gene confirmed the bacterium as Enterobacter cloacae. A minimal inhibitory concentration test showed multidrug resistance of the bacterium against 16 antibiotics. Polymerase chain reaction (PCR) and subsequent sequencing specifically for blaTEM, blaSHV, blaCTX-M, and plasmid-mediated ampC genes revealed positive results to blaTEM-1, blaCTX-M-15, and plasmid-mediated blaACT-1 genes, indicating that the isolated bacterium contains plasmids containing genes encoding extended-spectrum beta-lactamase and plasmid-mediated ampC beta-lactamase. After 1 month of treatment with antibiotics and levothyroxine, the cat’s condition improved; both the thyroid function test and the blood culture showed no abnormalities. This is the first report of community-acquired multidrug-resistant E. cloacae-induced euthyroid sick syndrome in a cat. By the prompt diagnostic procedures and properly selected antibiotic therapy, the cat was recovered from the multidrug-resistant bacterium-induced septicaemia.