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한구택 ( Ku Taek Han ),이형근 ( Hyung Kun Lee ),유순원 ( Sun Won Yoo ),송영훈 ( Young Hun Song ),최원영 ( Won Young Choi ),유기성 ( Ki Sung Ryu ),나종구 ( Jong Gu Rha ),송승규 ( Seung Kyu Song ),이헌영 ( Hun Young Lee ),김수평 ( S 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.12
Expression of four biologic markers known as p53, proliferating cell nuclear antigen(PCNA), c-myc, and epidermal growth factor receptor(EGFR), was studied immunohistochemically in 121 cases of cervical tissues, which included 15 cases of normal cervical tissues, 50 cases of squamous intraepithelial lesions(SIL), and 56 cases of invasive carcinomas. This study was designed to elucidate the roles of these factors in the genesis and progression of cervical neoplasia and to identify their association with clinical parameters such as cell type, tumor size, lymph node involvement, squamous cell carcinoma(SCC) antigen level, and prognosis. In additon, this study evaluates the differences of coexpression rates in normal cervical tissues, SILs, and invasive carcinomas. (1) The intensities of p53, c-myc, and EGFR expression and the rates of PCNA, c-myc, and EGFR expression in cervical SILs and invasive carcinomas were significantly higher than those in normal tissues (p<0.05 and p<0.01). But there were no significant differences between SILs and invasive carcinomas in both of intensities and rates of p53, PCNA, c-myc, and EGFR expression. (2) The intensities and rates of p53, PCNA, and c-myc expression in the invasive carcinomas did not correlate with clinical parameters including cell types, tumor sizes, lymph node involvements, see antigen, and prognosis. But only the intensity and rate of EGFR expression were significantly higher in cases with tumor size larger than 3 cm when it was compared with cases with tumor sizes smaller than 3 cm(p<0.01), in cases with lymph node involvement when it was compared with cases without lymph node involvement(p<0.05), and in cases with SCC antigen levels more than 2.5 ng/ml when it was compared with cases with SCC antigen levels less than 2.5 ng/ml(p<0.05). (3) C-myc immunoreactivity was significantly correlated with EGFR overexpression(p<0.01). (4) The rates of simultaneous expression of four factors were decreased significantly in normal tissues and significantly increased in invasive carcinomas(p<0.01). The coexpression rate of two factors or more was 6.7% in normal tissues, 62.7% in SILs, and 70.0% in invasive carcinomas and the simultaneous expression rate of three factors or more was none in normal tissues, 34.0% in SILs, and 47.5 % in invasive carcinomas. These results show that the immunohistochemical detection of p53, PCNA, c-myc, and EGFR expression will be useful in differentiating the normal tissues and cervical neoplasia. The results of simultaneous immunohistochemical detection of these four factor expression also suggest to contribute a better understanding of the genesis of cervical carcinoma and that coexpression of more factors indicate more aggressiveness in cervical neoplasia, and show that simultaneous detection of these factors can be used in the early detection of cervical neoplasia and to predict malignant transformation of cervical lesions.
MCF-7 세포주에서 Staurosporine 투여에 의한 세포주기 분기 변화와 p53단백 발현
남정(Jung Nam),여경아(Kyung A Yea),이해남(Hae Nam Lee),조현희(Hyun Hee Jo),류기성(Ki Sung Ryu),유영옥(Young Oak Lew),나종구(Jong Gu Rha),한구택(Ku Taek Han) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.3
N/A Objective : We investigated the effects on the cell cycle and p53 expression by the treatment of various concentrations of staurosporine to elucidate the molecular mechanism of staurosporine induced cell cycle arrest in MCF-7 cell line. Methods : Various concentrations of staurosporine were treated in MCF-7 cells cultured with RPMI 1640 media. The harvested cells were fixed and permeabilized with 1% paraformaldehyde and absolute methanol. Then the cells were stained indirectly with anti-p53 primary antibody and FITC conjugated goat anti-mouse(GAM)-IgG secondary antibody. Sequentially DNA were stained with 0.1% RNase and PI solution. These stained cells were analyzed by the standard FACScan flow cytometer. The obtained results were analyzed further with WinList 3.0, and ModiFit LT software program. Results : MCF-7 cells were arrested mostly in G1 phase of cell cycle at 5-10 nM of staurosporine, however, the cells were arrested in G2 phase at 20-100 nM of staurosporine. The expressions of p53 protein were higher in the MCF-7 cells treated with both concentrations of 10 nM and 100 nM of staurosporine compaired with the control cells. This suggests that the p53 may be involved in the mechanism of G1 and G2M arrest of cell cycle in MCF-7 cell. Conclusion : The points of arrest in cell cycle differred depending on the concentrations of staurosporine and these cell cycle arrests at G0G1 and G2M pahse were related with p53 protein expression. It suggested that these results could be extended to study for staurosporine to be usefull as a potential anti-tumor agent.
조윤성 ( Yun Sung Jo ),권지영 ( Ji Young Kwon ),임희순 ( Hee Sun Lim ),문영주 ( Young Joo Mun ),이상형 ( Sang Hyoung Lee ),제동성 ( Dong Sung Jae ),한구택 ( Gu Taek Han ),류기성 ( Ki Sung Ryu ) 대한산부인과학회 2006 Obstetrics & Gynecology Science Vol.49 No.12
Myoma is the most common tumor in gynecologic field. As ultrasonography because popular in antenatal care, the more cases of myoma and those adverse effects during pregnancy are more frequently detected. The management of myoma during pregnancy is conservative, but in rare circumstances, surgical intervention including myomectomy may be required. We have experienced a case of protruded subserosal myoma with the uterine cervix in midtrimester of pregnancy. The patient was managed surgically by transvaginal myomectomy and had successfully maintained pregnancy. We report a case of protruded subserosal myoma through pelvic floor in pregnancy with brief review of literatures.
절제수술로 치료한 자궁경부 상피내암과 미세침윤암에서 세포진과 조직진의 비교평가
윤수철 ( Soo Cheol Yoon ),공기환 ( Gi Hwan Gong ),정선안 ( Sun An Jung ),최영석 ( Young Seuk Choi ),유기성 ( Ki Sung Ryu ),한구택 ( Ku Taek Han ),나종구 ( Jong Gu Rha ),이헌영 ( Hun Young Lee ),김수평 ( Soo Pyung Kim ) 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.12
We evaluated 221 patients who were preoperatively diognosed as carcinoma in situ(CIS) and microinvasive carcinoma(MIC) from Jan. 1988 to Dec. 1995 at the Department of Obstetrics and Gynecology, St. Mary`s hospital, Catholic University Medical College. The data gained from 221 patients with sucessful tollow-up were thoroughly reviewed to determine the proper diagnosis and treatment model. They were treated by conization, total abdominal hysterectomy or type II hysterectomy. The final diagnoses of 127 patients who got one cytologic diagnosis at local clinic or our hospital were 80 CIS, 45 MIC and 2 mvasive carcinomas. In cytology, 47 case(58.8 %) of 80 CIS showed abnormal findings as class III(30 %), IV(22.5 %) and V(6.3 %). In 45 MIC, 33(73.3 %) cases showed abnormal finding as class III(35.6 %), IV(28.9 %) and V(8.9 %). 94 patients who got cytology twice at the local clinic or our department were finally diagnosed as 72 CIS and 22 MIC. By repeating cytology, we could reduce false positive rate, 12.5 % in CIS and 4.5 % in MIC, however repeat cytology had little diagnostic value. In comparison of colposcopy guided biopsy(CGB) to non-colposcopic blind biopsy, CGB was more predictive. In CIS, the accurate diaganosis rates were 77.6 %(52/67) in blind biopsy and 88.8 %(95/124) in CGB. In MIC, the accuracies were 71.8%(28/39) in blind biopsy and 100 %(33/33) in CGB. So colposcopic evaluation with optional conization was essential for pooper diagnosis and treatment. There were unfavorable histologic findings: vaginal extension(n=4; 1.8 %), lymphovascular space involvement(n=4; 1.8 %), perineural invasion(n=1; 0.5 %) and occult invasive cancer(n=2; 1.8 %). So conservative treatment and local destructive therapy were potentially dangerous in CIS and MIC and should be performed after precise evaluation in selected cases. For proper diagnosis and treatment, cytologic evaluation had limited clinical values and CGB with optional conization was needed.
임채춘(Chae Chun Rhim),노성래(Sung Rae Roh),김동주(Dong Joo Kim),송민경(Min Kyung Song),이재성(Jae Sung Lee),주하경(Ha Kyong Joo),박윤희(Youn Hee Park),유기성(Ki Sung Ryu),한구택(Gu Taek Han),이종건(Jong Kun Lee),이준모(Joon Mo Lee) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.8
The malignant lymphomas are neoplastic transformation of cells that reside predominantly in lymphoid tissues. The two major variants of malignant lymphoma are non-Hodgkin's lymphoma and Hodgkin's disease. Although both of these tumors infiltrate reticuloendothelial organs, their biologic and clinical behaviors suggest that they are probably not related. More than 90% of all cases of non-Hodgkin's lymphomas are of B-cell derivation. This observation is based upon the expression of B-lineage-restricted antigens as well as clonal rearrangements of immunoglobulin heavy and light chain genes. The malignant lymphoma localized in uterine cervix is rare and characteristically symptom-free expressed. We experienced a case of malignant lymphoma originated from uterine cervix, so we report with a brief of literature.
김정자,최현주,김영신,김기범,심상인,김수평,강창석,이헌영,나종구,백은정,한구택,여경아,권순만,이교영 대한부인종양 콜포스코피학회 1998 Journal of Gynecologic Oncology Vol.9 No.3
The incidence of malignant change of ovarian mature teratoma is 1∼2%. The majority is squamous cell cancer, the others was adenocarcinoma. Neuroepithelial tissue was frequently detected in mature cystic teratoma, but their malignant change was extremely rare. Only, two cases of neuroblastoma of ovarian teratoma were reported in the world. We report one case of neuroblastoma arising in ovarian mature teratoma with a brief review. Our case is the third reported one in the world.