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배양된 인간영양배엽세포에서 PGE2 와 Prostacyclin 이 혈관내피 성장인자와 염기성 섬유아세포 성장인자의 발현에 미치는 영향
노성래(Sung Rae Roh),김동주(Dong Joo Kim),안현영(Hyun Young Ahn),강미지(Mi Ji Kang),이방현(Bang Hyun Lee),이지현(Ji Hyun Lee),오민정(Min Jeong Oh),신종철(Jong Chul Shin),김수평(Soo Pyung Kim) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.8
N/A Background : Several angiogenic factors such as bFGF and VEGF have been shown angiogenesis of placenta. PGE2 and PGI2 may be important in successful establishment of pregnancy. Objective : We studied to investigate whether PGE2 and PGI2 regulate expression of VEGF and bFGF gene in the cultured human trophoblast cells. Methods : Human trophoblasts were isolated from the placenta of early gestation (6-12 weeks). Isolated trophoblasts were cultured in the different concentration of PGE2 and PGI2 and according to the different cultured time of PGE2 and PGI2, respectively. Total RNA was extracted and RT-PCR was performed. Result : Expression of bFGF was increased in 10-7M and 10-6M of PGE2 and was always increased in the all different concentration of PGI2. Four isoforms (VEGF121, VEGF165, VEGF189, VEGF206) were always expressed in the all different PGE2 and PGI2 concentration compared to the control group. However, there was no significant difference in the all different PGE2 and PGI2 concentration. In both PGE2 and PGI2 treatment group, expression of bFGF was decreased at 60 min compared to the control group and was gradually increased in time-dependent pattern. At 180 min, its expression was similar to the control group. Conclusion : Our data suggest that the expression of bFGF gene is influenced by cultured time and concentration of PGE2 and PGI2, although the expression of VEGF gene is not influenced.
임채춘(Chae Chun Rhim),노성래(Sung Rae Roh),김동주(Dong Joo Kim),송민경(Min Kyung Song),이재성(Jae Sung Lee),주하경(Ha Kyong Joo),박윤희(Youn Hee Park),유기성(Ki Sung Ryu),한구택(Gu Taek Han),이종건(Jong Kun Lee),이준모(Joon Mo Lee) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.8
The malignant lymphomas are neoplastic transformation of cells that reside predominantly in lymphoid tissues. The two major variants of malignant lymphoma are non-Hodgkin's lymphoma and Hodgkin's disease. Although both of these tumors infiltrate reticuloendothelial organs, their biologic and clinical behaviors suggest that they are probably not related. More than 90% of all cases of non-Hodgkin's lymphomas are of B-cell derivation. This observation is based upon the expression of B-lineage-restricted antigens as well as clonal rearrangements of immunoglobulin heavy and light chain genes. The malignant lymphoma localized in uterine cervix is rare and characteristically symptom-free expressed. We experienced a case of malignant lymphoma originated from uterine cervix, so we report with a brief of literature.
융모성 성선자극호르몬과 태반 락토젠이 태반 영양배엽세포의 분화와 성장에 미치는 영향
김동주 ( Dong Joo Kim ),신종철 ( Jong Chul Shin ),안현영 ( Hyun Young Ahn ),남궁성은 ( Sung Eun Namkoong ),백은정 ( Eun Jeong Baik ),노성래 ( Sung Rae Roh ),강미지 ( Mi Ji Kang ),배석년 ( Seog Nyeon Bae ) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.7
N/A Background: There are many factors that influence the differentiation and growth of trophoblasts. During differentiation of trophoblasts, two major hormones are secreted; human chorionic gonadotropin (hCG) and human placental lactogen (hPL). These two hormones are secreted in a peculiar pattern during pregnancy and function of these hormones is not yet fully understood. Also, it is not known whether these hormones directly influence the differentiation and growth of trophoblasts. On the other hand, it is known that choriocarcinoma cells are undifferentiated, so they are unable to form syncytiotrophblasts. And many factors may be associated with this inhibitory potential. Objective :The purpose of this study was to observe whether the hCG and hPL are associated with differentiation and growth of early placental trophoblasts and becoming malignant. Method: The hCG, hPL, IL-6 and insulin were added to cytotrophoblasts isolated from normal 8 to 10 gestational weeks placental tissues by a degree of concentration, and observed the secreted hPL concentration and morphological change to syncytiotrophoblasts daily. And it was performed in Bewo cells in same manner. Result: The increased hPL secretion was noted in hCG, hPL, IL-6 and insulin were added normal trophoblasts and this may result from differentiation of cytotrophoblasts to syncytiotrophoblasts. Also, morphological changes to syncytiotrohoblasts was observed at the same time. But, Increased hPL secretion and syncytiotrophoblasts formation was not detected in Bewo cells. Conclusion .In this study, it seems that hCG, hPL, IL-6 and insulin had an influence on differentiation and growth of normal trophoblasts. On the other hand, no changes in hPL secretion and morphology at the choriocarcinoma cell line tells us that defects of differentiation in choriocarcinoma is due to abnormalities of the receptors on hCG and hPL or a differentiation associated gene, not a defect of these hormones themselves.