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침전관이 장치된 배양기에서 B형 간염바이러스의 표면항원을 생성하는 동물세포의 연속배양
하석훈,한태욱,유리안,박완제,김현수 대한바이러스학회 1992 Journal of Bacteriology and Virology Vol.22 No.1
The hepatitis B virus surface antigen(HBsAg) was produced from recombinant mammalian cells named HV1-4. HV1-4 cell line was anchorage dependent cell and grew on the surface of micro-carriers. The aggregation of micro-carriers had been main limitation in large scale and long-term micro-carrier cultures. Therefore, we developed new cell sedimentation column that made large scale and long-term micro-carrier culture be possible. The sedirnentation column was consisted of a main sedimentation column and attached sub-sedimentation columns. The concentration of cells was increased high and rnaintained for 600 hrs, while the micro carriers were sedimented effectively.
재조합 사람 과립구 콜로니 자극인자인 CJ50001 의 중합체의 생물학적 활성과 급성독성에 관한 연구
하석훈(Suk Hoon Ha),이현수(Hyun Soo Lee),김기완(Ki Wan Kim),정종상(Jong Sang Sang),김달현(Dal Hyun Kim),임동문(Dong Moon Lim),김종호(Jong Ho Kim),조효진(Hyo Jin Cho),고형곤(Hyung Kon Ko) 한국응용약물학회 1998 Biomolecules & Therapeutics(구 응용약물학회지) Vol.6 No.1
CJ50001 is a recombinant human granulocyte colony-stimulating factor (rHuG-CSF) that stimulates the formation of neutrophils from bone marrow stem cells. It was produced in E. coli and purified through refolding and several processes. We produced CS970125(300) using purified CJS0001 and additives in order to test the stability of CJ50001. When CS970125(300) was stored at 50℃ for more than 1 week, high molecular weight proteins were formed and those proteins were detected by non-reducing SDS-PAGE, gel filtration HPLC, and Western blot. Those proteins showed single band at the same position of CJ50001 in reducing SDS-PAGE. These data indicated that those high molecular weight proteins were the multimers of CJ50001. In biological assays, in vitro and in vivo, the multimers did not have biological activity and inhibitory action to that of CJ50001. The mutimers did not induce toxicity in mice and rats in acute toxicity test. These results suggest that if CS970125(300) containing CJ50001 is stored at 50℃, CJ50001 will be the multimers that do not have biological activity and inhibitory effect to CJ50001 and do not induce acute toxicity.
Polio/Hepatitis B Chimeric 바이러스의 면역원성
한태욱,유리안,하석훈,박완제,김현수 대한바이러스학회 1992 Journal of Bacteriology and Virology Vol.22 No.2
To construct a polio chimeric virus expressing part of antigen of hepatitis B virus, we inserted a fragment of S gene of hepatitis B virus into a region of the gene encoding C3 epitope of poliovirus cDNA shuttle vector. The resulting plasmid designated pSBS-69 or RNA transcript derived from pSBS-69 was used to transfect vero cells. The cytopathic effect was observed in the transfected cells and viruses were purified from cell extract by ultracentrifugation. Rabbits were innoculated with polio/HB chimeric viruses. After boosting, antibodies were isolated frorn blood of the rabbits and purified by protein A affinity chromatography. Immunoenzyme assay showed that the antibodies had specificity with hepatitis B virus surface antigen (HBs Ag). The data suggested that polio/Hepatitis B chimeric virus had immunogenicity against HBs Ag.
A rare case of non-eosinophilic pleural effusion due to sparganum infection
김미혜,임창빈,최수용,하석훈,김학수 대한결핵 및 호흡기학회 2015 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.120 No.-
Sparganosis is a rare parasitic disease caused by migrating tape worm larva of the Spirometra family. . Pleural sparganosis is rarer. Infection in humans is usually caused by the ingestion of raw or inadequately cooked flesh of infected snakes or frogs. Till date, only 7cases of pleural sparganosis are reported. Most cases show related immunoserologic abnormalities with elevated eosinophil count in blood or pleural effusion. An 82-year-old woman with a history of old treated pulmonary tuberculosis was admitted with a large amount of right pleural effusion. She had been feeling something moving on the right upper quadrant of her chest for a long time. The complete blood count was normal without eosinophilia. The pleural fluid analysis showed exudative features with predominant lymphocytes and an adenosine deaminase level of 30.2 IU/L.. The serum IgE level was 5.53 IU/mL.. Percutaneous drainage was performed. During the procedure, a long tape worm like material was aspirated, which was identified as a sparganum body. Sparganum antibodies were not detected in the serum. The patient was administerd praziquantel with a single dose of 10 mg/kg/days for 2days. She later underwent video-assisted thoracic surgery examination, which confirmed that no worm body persisted. Herein, we report a very rare case of pleural sparganosis that presented without immunoserologic abnormalities.
강재구,백남진,김달현,하석훈,김제학,김현수 한국독성학회 1997 Toxicological Research Vol.13 No.3
In order to evaluate the mutagenic potential of CJ-50001 (recombinant human granulocytecolony stimulating factor), 3 sets of mutagenicity tests were performed. In the reverse mutation test using Salmonella typhimurium TA1535, TA1537, TA98 and TA100, CJ-50001 did not increase the number of revertant at any of the concentration tested in this study (500, 250, 125, 62.5 and 31.3 $\mu\textrm{g}$ plate). CJ-50001, at the doses of 200, 100 and 50 $\mu\textrm{g}$ /ml, did not increase the number of cells having structural or numerical chromosome aberration in cytogenetic test using Chinese Hamster Lung cells. In mouse micronucleus test, no significant increase in the occurrence of micronucleated polychromatic erythrocytes was observed in ICR male mice intraperitoneally administered with CJ-50001 at the doses of 5, 2.5 and 1.25 mg/kg. These results indicate that CJ-50001 has no mutagenic potential in these in vitro and in vivo systems.