구연발표 : 영유아 단체급식소 현장조사에 의한 영유아의 식품 및 영양소 섭취 실태

최은미 ( Eun Mee Choi ),신은경 ( Eun Kyung Sin ),정윤영 ( Yun Young Jung ),이난희 ( Nan Hee Lee ),최창한 ( Chang Han Choi ),정혜손 ( Hae Son Jung ),조은진 ( Eun Jin Cho ),성현이 ( Hyun I Sung ),이혜영 ( Hye Young Lee ),이은주 ( Eu 대한지역사회영양학회 2008 대한지역사회영양학회 춘-추계학술대회지 Vol.2008 No.2

전국 보육시설과 유치원의 급식 운영 및 영양교육 실태

최은미 ( Eun Mee Choi ),신은경 ( Eun Kyung Sin ),정윤영 ( Yun Young Jung ),이난희 ( Nan Hee Lee ),최창한 ( Chang Han Choi ),정혜손 ( Hae Son Jung ),조은진 ( Eun Jin Cho ),성현이 ( Hyu Ni Sung ),이혜영 ( Hye Young Lee ),이은주 ( Eu 대한지역사회영양학회 2008 대한지역사회영양학회 춘-추계학술대회지 Vol.2008 No.2

유아교사와 예술치료 : 유아교사와 예술치료사의 역할, 자질 및 환경 비교를 중심으로 Centered on the Role and Qualification of the Early Childhood Teachers and the Art Therapists and the Environment of Play and Therapy

최은미 ( Eun Mee Choi ) 한국아동교육학회 2008 아동교육 Vol.17 No.2

유치원교육과정에 따른 전통문화교육프로그램 개발

최은미 ( Eun Mee Choi ),이승원 ( Seung Won Lee ),이선이 ( Son Lee Lee ) 미래유아교육학회 2005 미래유아교육학회지 Vol.12 No.4

β - Carotene 공급이 흰쥐 간 조직의 지질대사와 관련효소의 활성에 미치는 영향

최은미(Eun-Mee Choi),박정륭(Jyung-Rewng Park),서정숙(Jung-Sook Seo) 한국식품영양과학회 1994 한국식품영양과학회지 Vol.23 No.5

β-carotene은 비타민 A 활성형인 retinol로 전환되어 그 기능을 수행하는 영양소로 알려져있지만 β-carotene 자체로서의 생리적, 영양학적 중요성이 부각되고 있다. 본 연구에서는 β-carotene을 수준별로 공급하여 체내의 지질대사와 항산화 효소의 활성에 미치는 영향을 살펴보고자 계획되었으며 얻어진 결과는 다음과 같다. 간 미토콘드리아에서의 과산화지질의 함량은 제한군과 과잉 공급군이 다른 처리군들에 비해서 상대적인 증가를 보였고 BC 2군에서 가장 낮은 값을 나타내었다. SOD의 활성은 β-carotene 제한군이 공급군에 비해서 유의적으로 증가하여 SOD 활성에 대한 β-carotene의 조절효과를 보여주었고, catalase와 GSH-Px의 활성에 대한 β-carotene의 조절효과를 보여주었고 catalase와 GSH-Px의 활성은 과잉 공급군의 경우 가장 낮은 값을 보여주었다. β-carotene 공급에 따른 지질 함량의 변화는 간의 경우에 총지질 함량은 β-carotene 공급에 따라 감소하였고 중성지질은 전 군에서 유의적인 차이가 보이지 않았으며 인지질은 BC 2군과 BC 3군에서 유의적으로 낮은 값을 보였다. 콜레스테롤 함량은 BC 1군에서 가장 낮은 값을 보였다. 혈장에서는 β-carotene 공급이 증가함에 따라 중성지질의 함량은 증가하는 경향이었고 HDL-콜레스테롤은 BC 4군에서 가장 낮은 값을 나타내었다. HDL-콜레스테롤 함량비는 β-carotene 공급에 따라 증가되었으나 과잉공급군에서 제한군과 같은 수준으로 감소되었다. 이상의 결과에서와 같이 12,000㎎의 β-carotene을 급여한 군에서는 지질대사 관련효소 활성도와 체내 지질함량이 적정량을 급여한 군에 비해 크게 변화하였으나 10~1,200㎎을 급여한 실험군들 사이에서는 큰 차이를 나타내지 않아 β-carotene의 정량적 평가를 위한 지속적인 연구가 필요하다고 본다. This study was designed to investigate the effect of dietary β-carotene level on the lipid metabolism and lipid peroxide metabolizing enzyme activities in rats. Male Sprague-Dawley rats were fed on diets containing five levels of β-carotene (0, 10, 120, 1200, 12000㎎/㎏ diet ; BC 0, BC 1, BC 2, BC 3, BC 4 group). The rats were sacrificed after 7 weeks of the feeding periods. Lipid peroxide value of mitochondrial fraction of rat liver was elevated in β-carotene restriction group when compared to β-carotene groups. Superoxide dismutase activity increased significantly by β-carotene supplementation. Both catalase and glutathione peroxidase activities were reduced with increasing β-carotene supplementation, except only β-carotene restriction group. In liver, the contents of total lipid and cholestero decreased by β-carotene supplementation but triglyceride content was not different among treatment groups. HDL-and total cholesterol ratio in plasma of 12,000㎎ β-carotene group decreased, and was similar to that of β-carotene restriction group.

아크릴릭에 의한 네일형태 변화가 조갑감입증 개선에 미치는 영향

최은미 ( Eun Mee Choi ),정영희 ( Young Hi Jeong ),김기영 ( Ki Young Kim ) 대한미용학회(구 대한미용과학회) 2011 대한미용학회지 Vol.7 No.1

Ingrowing toenail is one of the most common nail complaints. In nail care, the acrylic nail is the method for making artificial nails with hardening of the shape within a certain time after mixing a blend of 3 other materials-polymer methyl methacrylate polymer powder, monomer the liquid acryl and catalyst. The acrylic nail which makes the artificial nails can be used in improvement of ingrown nails. In this study, it was conducted the experiment during 8 weeks that the square type acrylic nail treatment on the big toenail of 12 ingrown nail women without infection. Before the experiment and 2, 4, 6, 8 weeks later of that, the improvement effect of ingrown nail was investigated by measuring the width of C-curve and observation of the change of C-curve shape and question about pain. As a result, the width of C-curve of right big toenail was significantly increased 24.22±6.11% from 11.50±1.23 mm to 14.24±1.24 mm 8 weeks later. And that of left big toenail was increased 21.76±7.33% from 11.57±1.43 mm to 14.01±1.19 mm 8 weeks later. According to the naked eye observation, there was stronger pain at the beginning of experiment than before the starting of that. But 3~4 days later, the participants answered that there was no pain and 2, 4, 6, 8 weeks later, the width of C-curve was increased in measurement. In accordance with these results, it is confirmed that the ingrown nail treatment is valid on pain relief of ingrown nail. Thus, it is suggested that the acrylic nail treatment is worth of being used in the field of nail cosmetology because that is noninvasive method and is possible to adjust pain of ingrowing and to correct the shape of toenail in a brief time.

개의 적출방광 평활근에서 Imipramine과 $K^+$ 통로 봉쇄제와의 상호작용

허준영,최은미,최형철,하정희,이광윤,김원준,Huh, Joun-Young,Choi, Eun-Mee,Choi, Hyoung-Chul,Ha, Jeoung-Hee,Lee, Kwang-Youn,Kim, Won-Joon 대한약리학회 1995 대한약리학잡지 Vol.31 No.2

The study was undertaken to examine the possibility of the involvement of $K^+$ channels in the mechanism of relaxant-action of imipramine on the isolated canine detrusor muscle strips. Canine urinary bladder were isolated, and smooth muscle strips of 15 mm long and 2 mm wide from the mid-portion of anterior wall were made in the Tyrode solution of $0{\sim}4^{\circ}C$. The strips were prepared for isometric myography in Biancani's isolated muscle chamber containing 1 ml of Tyrode solution, which was maintained with pH 7.4 by aeration with $95%\;O_2/5%CO_2\;at\;37^{\circ}C$. RP 52891, a non-specific $K^+$ channel opener, concentration-dependently suppressed the spontaneous phasic contractions of the detrusor strips. Imipramine, a tricyclic antidepressant, also reduced the spontaneous contractions in a concentration-dependent manner. RP 52891 was more potent than imipramine(p<0.05), and Imipramine was more efficient than RP 52891(p<0.05).Procaine, a voltage-dependent $K^+$ channel blocker, glibenclamide, an ATP-dependent $K^+$ channel blocker, and apamin, a calcium-dependent $K^+$ channel blocker antagonized the relaxant effect of RP 52891, but not of imipramine. Imipramine reduced the electric field stimulation (EFS) -induced contractions concentration-dependently. None of the $K^+$ channel blockers employed for this study, procaine, glibenclamide or apamin antagonized the inhibitory action of imipramine on the EFS-induced contraction. These results suggest that in canine detrusor, the $K^+$ channels of the characteristics of voltage-dependent, ATP-dependent and/or calcium-dependent are exist, and the inhibitory action of imipramine on the contractility of the detrusor is independent from the $K^+$ channels.

모래쥐에서 뇌의 허혈/재관류에 의한 산화성 스트레스 형성과 HSP70의 발현

박영미(Young-Mee Park),김철훈(Chul-Hoon Kim),도윤정(Yun-Jeong Do),최은미(Eun-Mi Choi),안영수(Young-Soo Ahn) 대한약리학회 1996 대한약리학잡지 Vol.32 No.3

허혈/재관류 뇌손상에서 활성산소류의 역할이 중요시되고 있다. 본 연구에서는 모래쥐의 총경동맥을 묶었다 풀어줌으로써 실험적 허혈/재관류 손상을 유도하고 산화성 스트레스 발생 유무와 이러한 산화성 스트레스가 세포손상으로 연결되는지를 알아보고자 하였다. 해마는 뇌조직 중에서도 특히 산화성 스트레스에 취약한 부분이므로 해마에서 얻은 조직을 대뇌피질에서 얻은 조직과 비교분석하였다. 또한, 이들 부위에서 heat shock protein 70(HSP70)의 발현이 허혈/재관류 손상에 미치는 영향도 검색하고자 하였다. 허혈/재관류에 의한 산화성 스트레스의 지표로써 글루타치온 산화정도, GSSG/(GSH+2xGSSG)를 측정하였을 때 주로 해마에서 산화지표가 상승됨을 관찰하였다. 한편 산화성 스트레스가 세포손상으로 연결되는지를 알아보고자 지질과산화물을 측정하였다. 두 부위 모두에서 지질과산화물 형성의 증가가 있었으며 대뇌피질에서보다 해마에서 더 증가됨을 알 수 있었다. 지질과산화물 형성의 정도나 시간적 변화양상이 글루타치온 산화의 그것들과 유사하였다. 이러한 결과들은 허혈/재관류에 의해 산화성 스트레스가 형성되며 동시에 이러한 산화성 스트레스가 세포 손상을 초래함을 보여준다. 또한 산화성 스트레스 및 산화성 세포손상 정도가 대뇌피질보다는 해마에서 더 큰 것을 알 수 있었다. 그러나, 피질과 해마에서 HSP70의 기초발현(basal level) 정도는 차이가 없었다. 이는 해마의 취약성이 HSP70 발현 결핍에 기인하지 않았음을 나타낸다. 반면 허혈/재관류에 의한 HSP70의 발현유도는 해마조직에서 제대로 이주어지지 않았고 northern blot결과 이는 전사단계에서의 부친에 의한 것으로 나타났다. 이러한 결과들로 볼 때 허혈/재관류에 의한 뇌손상에서 HSP70 유도정도를 측정하는 것이 세포의 취약성을 예측할 수 있는 지표로 유용하게 사용될 수 있을 것으로 사료된다. A critical role of oxygen-derived free radicals has been implicated in ischemia/reperfusion (I/R)-induced brain damage. In this study, we have produced experimental I/R to the brains of Mongolian gerbil (Meriones unguiculatus) by a transient occlusion and release of the common carotid arteries. We have attempted to determine whether the oxidative stress is generated upon I/R and whether this oxidative stress is linked to the cell damage. Since hippocampus has been suggested as one of the most vulnerable regions of the brain to the oxidative stress, we analyzed samples from hippocampus in comparison with those from cortex. In addition, we have examined the expression of heat shock protein 70kD species (HSP70) in these regions in order to evaluate a possible role of this protein in I/R-induced brain damage. To determine whether the oxidative stress is produced upon I/R, we measured the glutathione oxidation, GSSG/ (GSH + 2xGSSG), as an index of oxidative stress. We found an increase of the glutathione oxidation primarily in hippocampus upon I/R. To determine whether this oxidative stress is linked to the cell damage, we measured the degree of lipid peroxidation upon I/R. We found an increase of lipid peroxidation in both regions. However, the magnitude of increases was greater in hippocampus than in cortex. In addition, we found that changes in both the magnitude and the temporal patterns of glutathione oxidation closely correlated with those of lipid peroxidation. Our study provides biochemical evidences that the oxidative stress is generated upon I/R and this oxidative stress is linked to the oxidative cell damage. Our study also provides evidences that the degree of oxidative stress as well as oxidative cell damage is greater in hippocampus than in cortex. We could not find difference in the basal level of HSP70 expression between hippocampus and cortex, indicating that the intrinsic vulnerability of hippocampus cannot be explained by the lower level of HSP70 expression. We did find, however, that the induction of HSP70 expression upon I/R was impaired in the hippocampus. This impairment appeared to be at the transcriptional level. These results suggest that the measurement of HSP70 induction may be employed as a useful predictor of differential cellular susceptibilities to the I/R-induced brain damage.

기관근의 수축성에 대한 말초성 Benzodiazepine 수용체의 역할

류한영,최형철,최은미,손의동,이광윤,김원준,하정희,Rhyu, Han-Young,Choi, Hyung-Cheol,Choi, Eun-Mee,Sohn, Uy-Dong,Lee, Kwang-Youn,Kim, Won-Joon,Ha, Jeoung-Hee 대한약리학회 1997 The Korean Journal of Physiology & Pharmacology Vol.1 No.6

Non-neuronal high affinity binding sites for benzodiazepines have been found in many peripheral tissues including cardiac muscle and vascular smooth muscle, and have been designated as 'peripheral benzodiazepine receptor'. Benzodiazepines have been shown to induce relaxation of the ileal, vesical, and uterine smooth muscles. However, it is still unclear about possible involvement of peripheral benzodiazepine receptor on the contractility of trachealis muscle. This study was performed to investigate the role of the peripheral benzodiazepine receptor on the contractility of canine trachealis muscle. Canine trachealis muscle strips of 15 mm long were suspended in an isolated organ bath containing 1 ml of physiological salt solution maintained at $37^{\circ}C$, and aerated with $95%\;O_2/5%\;CO_2$. Isometric myography was performed, and the results of the experiments were as follows: Ro5-4684, FGIN-1-27 and clonazepam reduced a basal tone of isolated canine trachealis muscle strip concentration dependently, relaxant actions of RoS-4684 and FGIN-1-27 were antagonized by PK11195, a peripheral benzodiazepine receptor antagonist. Flumazenil, a central type antagonist, did not antagonize the relaxant action of Peripheral type agonists. Saturation binding assay of [3H]Ro5-4864 showed a high affinity$(Kd=5.33{\pm}1.27nM,\;Bmax=\;867.3{\pm}147.2\;fmol/mg\;protein)$ binding site on the canine trachealis muscle. Ro 5-4684 suppressed the bethanechol-, 5-hydroxyoyptamine- and histamine- induced contractions. Platelet activating factor (PAF) exerted strong and prolonged contraction in trachealis muscle strip. Strong tonic contraction by PAE was attenuated by Ro 5-4684, but not by WEB 2086, a PAF antagonist. Based on these results, it is concluded that the peripheral benzodiazepine receptor mediates the inhibitory regulation of contractilty of canine trachealis muscle.

고주파기기 관리가 중년여성의 얼굴피부 유·수분 조절과 피부색 개선에 미치는 효과

이종신 ( Jong Sin Lee ),최은미 ( Eun Mee Choi ),김기영 ( Ki Young Kim ) 한국미용학회 2016 한국미용학회지 Vol.22 No.4

High frequency (HF) apparatus use for skin care in area of medical and cosmetics. In this study, regulation of sebum·moisture and skin color improvement effect investigated in middle age women by HF treatment. The face of subjects was treated two times/week in six weeks, and content of sebum·moisture and skin color such as melanin, erythema, lightness, redness and yellowness were measured before and after HF treatment by sebameter, mexameter and chromameter. In the result, value of sebum and moisture increased significantly in 6 weeks by HF treatment(p<0.05) and value of sebum and moisture in T-zone was significantly higher than that of U-zone. Content of melanin and erythema decreased significantly during the 6 weeks observation period by HF treatment(p<0.05). Content of lightness increased significantly from one week until 6 weeks by HF treatment(p<0.05), and in contrast content of redness decreased significantly from one week until 6 weeks by HF treatment(p<0.05). But content of yellowness showed almost no change during the 6 weeks observation period. In conclusion, the HF treatment can be improved sebum·moisture regulation and skin color, and may be useful apparatus for aging skin care.