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          국내 거베라 육종계통 형질전환 기초 조건 확립

          이혜영,이기정,전은희,신상현,이재헌,김도훈,정대수,정용모,조용조,김정국,정영수,Lee, Hye-Young,Lee, Ki-Jung,Jeon, Eun-Hee,Shin, Sang-Hyun,Lee, Jai-Heon,Kim, Doh-Hoon,Chung, Dae-Soo,Chung, Yong-Mo,Cho, Yong-Cho,Kim, Jeong-Kook,Chung, You 한국식물생명공학회 2006 식물생명공학회지 Vol.33 No.1

          본 연구에서는 거베라 형질전환 기초기술개발을 위한 연구를 수행하였다. 경남원예화훼시험장에서 육성된 국내 거베라 18품종 중, Agrobacferium 감염에 순응적인 품종을 선발하기 위하여 18개의 국내 거베라 품종을 스크린하여 011, 016, GM023 등 12개의 순응형 계통을 스크린 하였으며 효율적인 형질전환을 위한 제균 항생제로는 cefotaxime이 선택되었다. 더 많은 캘러스 유도를 위한 전처리 배지의 호르몬 농도는 BA 2 ppm, Zeatin 2 ppm, IAA 0.2 ppm (2 x media)이 효율적이었고 재분화 배지의 호르몬 농도도 전처리 배지 호르몬의 농도와 동일하게 BA 2 ppm, Zeatin 2 ppm, IAA 0.2 ppm (2 x media)에서 효율적이었으나 GUS 발현에는 다른 차이가 없었다. 고효율 형질전환 체계 확립을 위하여 네 가지 다른 전처리 기간 처리와 두 가지 다른 Agrobacterium tumerfaciens 사용, 두 가지 다른 공배양 기간을 처리한 결과 전처리 하지 않은 엽병과 엽신을 LBA4404 Agrobacterium tumerfaciens을 사용하여 5일 간 공배양 기간을 가졌을 때와 dipping 처리하여 접종한 엽병과 엽신에서 높은 GUS 발현빈도를 나타냈다. Gerber (Gerbera hybrida) is a valuable ornamental species grown as a potted plant and cut flowers. However, genetic variability within the gerbera genus is very limited. So it is absolutely needed to introduce and widen genetic resources into gerbera lines by genetic transformation. For the purpose, 18 Korean gerbera lines were screened to establish Agrobacterium-mediated genetic transformation procedure. In an experiment to select Korean gerbera lines which are amenable to Agrobacterium-inoculation, 12 lines turned out to be positive in Agrobacterium-inoculation. More callus were produced from BA 2ppm, Zeatin 2ppm, IAA 0.2ppm in pre-culture and regeneration medium (2X media) but there was no difference in the frequency of GUS expression rate. In another experiment to find out optimal condition for highly efficient Agrobacterium-inoculation, petiole and leaf explants have been treated with four different pre-culture periods, two different co-culture periods and two different Agrobacterium strains. As a result, high GUS expression has been showed from petiole and leaf explants treated no pre-culture period with LBA4404 Agrobacterium tumerfaciens, 5 day co-culture period and dipping treatment.

        • KCI등재
        • KCI등재

          인산(燐酸) ESTER계(系) 접착성(接着性) 시멘트의 치수반응(齒髓反應)에 관(關)한 실험적(實驗的) 연구(硏究)

          이혜영,최대균,최부병,박남수,Lee, Hye-Young,Choi, Dae-Gyun,Choi, Boo-Byung,Park, Nam-Soo 대한치과보철학회 1986 대한치과보철학회지 Vol.24 No.1

          It is considered that etching solution or material itself of phosphoric ester cement will induce not a little pulpal irritation, if applied directly onto unsealed dentinal tubules. This study was designed to confirm above consideration by comparing two different conditions between $Ca(OH)_2$-based and non-$Ca(OH)_2$-based group. Posterior teeth of 15 male dogs were selected for this experiment. One experimental group was filled with cement after $Ca(OH)_2$-basing and enamel-etching, the other experimental group after enamel etching without $Ca(OH)_2$-basing. And both of two experimental groups were observed at 2 hours, 15 hours, 3 days, 1 week, 2 weeks, 4 weeks, and 6 weeks after filling. The findings reached to the following conclusions histologically. 1. In both groups, the damaged odontoblasts were atrophied and eventually disappeared. 2. In non-based group at early stage, odontoblasts were severely atrophied and defective areas were appeared between odontoblast cell layers. However, in based group, the odontoblasts were arranged slight irregularly. 3. In non-based group, a small number of undifferentiated cells below the odontoblast cell layers started to appear at 1 week after filling. However, in based group, the undifferentiated cells were appeared at 15 hours after filling. 4. In non-based group, formation of reparative dentin was not begun until late stage of experiment. However, in based group, formation of reparative dentin matrix was begun at 2 weeks after filling and very thickened reparative dentin was formed at 6 weeks after filling. 5. In odontoblast cell layers of both groups, dilated capillaries were observed. 6. Argyrophilic fibers were reticularly condensed in zone of Weil, and they were connected to the pulp tissue and dentin.

        • KCI등재

          뇌성마비아동 어머니의 스트레스 및 대처방식 조사

          이혜영,김경,차용준,Lee, Hye-Young,Kim, Kyoung,Cha, Yong-Jun 대한물리치료학회 2012 대한물리치료학회지 Vol.24 No.2

          Purpose: The goal of this study was to analyze the degree of stress and methods of coping with stress in mothers of cerebral palsied children and to provide a basic resource for the development of policies to improve the mental health of mothers with cerebral palsied children. Methods: 85 mothers with cerebral palsied children and 77 mothers of normal children completed a self-administered questionnaire that evaluated the degree of stress and methods of coping with stress. Cronbach's ${\alpha}$ score was used to determine the internal consistency of the acquired data and the discriminated validity was estimated by Pearson's correlation coefficient. Independent t-tests were conducted to compare the degree of stress and method of coping with stress between mothers of cerebral palsied children and mothers of normal children and one-way ANOVA was performed to analyze the effect of generalized characteristics on stress in mothers of cerebral palsied children. Results: The degree of stress in terms of anxiety response and roles as mother for mothers with cerebral palsied children was higher than mothers with normal children. However, there was no significant difference between two group in regards to the methods of coping with stress during a stressful episode. The degree of stress for mothers with cerebral palsied children was greater when the child was younger. Conclusion: Mothers of cerebral palsied children had higher stress than that of mothers with normal children and felt more stress when their child was younger. Therefore, these results suggest that health-based policies should be developed to improve the mental health of mothers with cerebral palsied children.

        • KCI등재

          우리나라 소아 물리치료사의 임상적 추론

          이혜영,이인희,김경,Lee, Hye-Young,Lee, In-Hee,Kim, Kyoung 대한물리치료학회 2012 대한물리치료학회지 Vol.24 No.5

          Purpose: The purpose of this qualitative study was to understand the application of clinical reasoning throughout clinical and therapeutic knowledge of clinical pediatric physical therapists under physician prescriptions. Methods: Purposive sampling techniques were used in selection of nine clinical pediatric physical therapists in South Korea. Open and semi structured interviews were conducted, and were thoroughly examined and reviewed, followed by analysis of the clinical reasoning portion. Results: Pediatric physical therapists have been influenced by information gathering, physical function and evaluation of their patients, hypothesis-orientation, and re-evaluation and self-monitoring. Novice physical therapists were more dependent on physician prescriptions and parent's hope than the result of their own evaluation and critical pathway. Middle experienced pediatric physical therapists were more dependent on rapport with children and their parents. Highly experienced pediatric physical therapists were more reliant on hypothesis-orientation and self-monitoring. As reports on clinical experience of pediatric physical therapists have accumulated, clinical reasoning of pediatric physical therapists has been influenced by their experience, such as education, clinical pathology conference, their own clinical experience and rapport with patients and their parents than physicians' prescriptions. Conclusion: The findings of the current study are generally consistent with existing research on clinical reasoning. The results of the current study may be used by educators for enhancement of clinical reasoning abilities and knowledge of students or novices as well as development of a guide for use by suitable novices or students, and could provide important information for use in physical therapy practice and research.

        • KCI등재

          국내 학술지 논문의 주제어를 통한 학술연구분야 관계분석

          이혜영,곽승진,Lee, Hye-Young,Kwak, Seung-Jin 한국비블리아학회 2011 한국비블리아학회지 Vol.22 No.3

          본 연구의 목적은 국내 연구자들의 연구 성과물인 학술지 논문에 나타난 학문분야의 상호관계성과 연관성에 대한 연구로 연구동향에 대한 시사점을 제시하는 것이다. 학술논문을 이용한 계량적 연구는 주로 논문의 초록, 자동추출 키워드, 논문의 풀텍스트를 활용하는 것이 일반적이나 본 연구는 실험대상 학술논문의 저자가 제시한 주제어에 초점을 두었다. 실험대상 자료는 2009년도 한국연구재단 등재학술지 80종으로 학술논문 7,616건, 주제어 58,143건이다. 자료 분석결과 공학, 농수해양, 복합학, 사회, 예술체육, 의약학, 인문, 자연과학 등의 8개 각 연구분야는 주제어 사용에서 차이점을 보였다. 특히 공학분야의 주제어가 다른 연구 분야에서도 주제어로 많이 사용되고 있었다. 또한 주제어 네트워크를 통해 연구분야를 클러스터링 한 결과, 3개 클러스터를 얻을 수 있었다. 클러스터A는 '공학', '자연과학', '사회', '복합학', '인문', 클러스터B는 '의약학', '예술체육', 클러스터C는 '농수해양'이었다. The purpose of this paper is to analyze the interrelation among research areas based on domestic journal papers, achievements of korea researchers. Generally, the content of papers is appeared through abstracts, subjects, full-text and so on. This paper is focused on subject terms of Domestic journal papers. The experimental data are 80 domestic journals, 7,616 papers and 58,143 subject terms and papers published in 2009. As the result, it was different to use subject terms on each research area: Engineering, Agriculture & Oceanography, Interdisciplinary Science, Social Science, Arts & Physical Education, Medicine & Pharmacology, Humanities and Natural Science. Subject terms of Engineering have used the most in the other research areas in aspect of term co-occurrence. The 8 research areas were grouped in 3 clusters: C1(Engineering, Natural Science, Social Science, Interdisciplinary Science, Humanities), C2(Medicine & Pharmacology, Arts & Physical Education), and C3(Agriculture & Oceanography).

        • 캠퍼스 상호대차서비스 전산화에 따른 성과 분석

          이혜영,Lee, Hye-Young 한국과학기술정보연구원 과학기술정보센터 2009 정보관리연구 Vol.40 No.4

          많은 도서관은 한정된 자원을 극복하고 이용자의 정보요구를 충족시키기 위하여 상호대차서비스를 활용한다. 상호대차서비스는 도서관 서비스의 소유 소장의 개념에서 공유 접근의 개념으로 변화를 말하는 것으로, 상호대차서비스 평가 및 그 필요성을 재고할 필요가 있다. K대학은 단행본 중심의 캠퍼스 상호대차서비스는 오랫동안 제공하였는데, 2003년부터 온라인서비스를 제공하는 등의 노력을 지속적으로 하였다. 본 연구는 상호대차서비스의 전산화가 서비스에 미치는 영향을 분석하기 위한 것으로 9년간의 서비스운영 실적을 비교분석하였다.시스템 발전 단계에 따라 상호대차 신청과 도서 대출 건수가 급격히 증가하는 것을 볼 수 있었다. 또한 서울캠퍼스 소속 이용자의 상호대차신청건수가 2001년 전체 도서신청건수 중 12%이었던 것이 2008년 무려 75%까지 상승한 것을 확인할 수 있었다. 상호대차 처리시간도 2004년의 17% 수준으로 떨어지는 등 많은 개선 결과를 보였다. Generally, we used to provide ILL to users for overcoming the limited resource and satisfying user's desires for information. ILL makes library service's paradigm change from ownership to sharing. K-university Library has been providing Campus inter-library loan as ILL to users for long time and continually trying to improve the service's quality. This case study is to analysis the performance of campus inter-library loan in the past 9 years. As the related automation system was developed and upgraded, the service requests have been increasing annually. Campus inter-library loan requests were 12% of total book requests in 2001, and then were 75% of total book requests in 2008. The transaction time was reduced until 17% of 2004.

        • KCI등재

          학령기 정상 아동의 호흡 특성과 신체 조건에 관한 상관분석

          이혜영,강동연,김경,Lee, Hye Young,Kang, Dong Yeon,Kim, Kyoung 대한물리치료학회 2013 대한물리치료학회지 Vol.25 No.5

          Purpose: Respiratory is an essential vital component for conservation of life in human, which is controlled by respiratory muscles and its related neuromuscular regulation. The purpose of this study is to assess lung capacity and respiratory pressure in healthy children, and to investigate relationship and predictability between respiratory pressure and other related respiratory functions. Methods: A total of 31 healthy children were recruited for this study. Demographic information and respiratory related factors were assessed in terms of body surface area (BSA), chest mobility, lung capacity, and respiratory pressure. Correlation between respiratory pressure and the rested variables was analyzed, and multiple regression using the stepwise method was performed for prediction of respiratory muscle strength, in terms of respiratory pressure as the dependent variable, and demographic and other respiratory variables as the independent variable. Results: According to the results of correlation analysis, respiratory pressure showed significant correlation with age (r=0.62, p<0.01), BSA (r=0.80, p<0.01), FVC (r=0.80, p<0.01), and FEV1 (r=0.70, p<0.01). In results of multiple regression analysis using the backward elimination method, BSA and FVC were included as significant factors of the predictable statistical model. The statistical model showed a significant explanation power of 71.8%. Conclusion: These findings suggest that respiratory pressure could be a valuable measurement tool for evaluation of respiratory function, because of significant relationship with physical characteristics and lung capacity, and that BSA and FVC could be possible predictable factors to explain the degree of respiratory pressure. These findings will provide useful information for clinical assessment and treatment in healthy children as well as those with pulmonary disease.

        • KCI등재

          임상연구환자를 돌보는 병동간호사와 임상연구간호사의 역할기대와 협력 인식

          이혜영,김상희,Lee, Hye-Young,Kim, Sanghee 한국산학기술학회 2021 한국산학기술학회논문지 Vol.22 No.6

          입원을 경험하는 임상연구 환자가 증가하고, 이에 따라 이들을 돌보는 간호사들의 업무가 증가하고 있으나, 모호한 역할 경계로 혼란이 발생하고 있다. 본 연구는 임상연구업무 중 병동간호사의 참여가 가능한 직접실무 제공업무, 교육 및 상담업무, 옹호 및 조정업무에 대한 역할기대와 병동에서의 협력인식 및 협력 장애요인을 파악하기 위한 조사연구이다. 대상자는 병동 간호사 66명, 임상연구간호사 65명이었다. 자료수집은 SAS 9.3통계 프로그램을 이용하여 기술 통계, two-sample t-test, 𝑥<sup>2</sup> test 로 분석하였다. 연구결과, 임상연구 간호업무의 직접실무 제공영역, 교육 및 상담영역, 옹호 및 조정영역을 포함한 역할기대 점수는 병동간호사는 4.56±0.41점, 임상연구간호사는 3.83±0.60점으로 서로에 대한 역할기대 점수가 높게 나타났다. 통계적으로 병동간호사의 역할기대가 유의하게 (p<.001) 임상연구간호사보다 더 높게 나타났다. 두 군 간의 협력 인식은 5개 항목에서 유의한 차이가 나타났다. 협력장애요인으로는 업무과중과 임상연구에 대한 인식부재가 높은 순위로 나타났다. 높은 역할기대는 역할 갈등의 요소가 된다. 그러므로, 새로운 임상연구 간호업무에서의 병동간호사와 임상연구간호사의 역할 정립을 위하여 절차 마련과 효과적인 실무 관행의 정착, 공식적 시스템 운영이 필요할 것으로 사료된다. 두 군 간의 협력을 증진하기 위해서는 서로를 동반자로 생각하고 상대에 대한 전문성을 인정할 수 있는 상호 신뢰하고 협력하는 문화가 형성되어야 할 것으로 사료된다. This was an investigative study to identify the role expectations for clinical research related tasks that ward nurses can participate in and the awareness of cooperation between ward nurses and clinical research nurses (CRNs) and the obstacles for the same. The subjects were 66 ward nurses and 65 CRNs. The data were analyzed using descriptive statistics, the two-sample t-test, and the 𝑥<sup>2</sup> test on SAS 9.3. As a result of this study, 4.56±0.41 ward nurses and 3.83±0.60 CRNs were found to have high role expectations of each other and statistically significant role expectations for ward nurses (p<.001). Significant differences were seen in the five categories of cooperation recognition between the two groups. Among the factors for cooperative disabilities, excessive work and lack of awareness of clinical research were ranked high. High role expectation becomes an element of role conflict. To organize the role of ward nurses and clinical research nurses in the new clinical research nursing work, procedures, establishing of effective practices, and the proper operation of an official system are expected to be necessary. To promote cooperation between the two groups, a culture of mutual trust and cooperation should be formed to enable each group to think of the other as partners and to recognize their expertise.

        • KCI등재

          지구 관측자료 공유를 위한 메타데이터 연구

          이혜영,곽승진,Lee, Hye-Young,Kwak, Seung-Jin 한국문헌정보학회 2007 한국문헌정보학회지 Vol.41 No.2

          The purpose of this study is to design the metadata for sharing and efficiently using the informations of Earth Observations in Korea. Recently the GEO(Group on Earth Observations) has been starting to manage all nation's informations in a way that benefits the environment as well as humanity by taking a pulse of the plants. For that reason, we have to construct the infra-system to manage and integrate the 12 social benefit areas in Korea; disaster, health, energy, climate, water, biodiversity, agriculture, forest, ocean, space information and geographical information system(GIS). But these informations have only been managed with varied metadatas in each area of observation, and then it is difficult to integrate varied informations. In this study, we solve the problem with meta-metadata. 본 연구는 지구 관측자료 공유 및 효율적 활용을 위한 통합 메타데이터를 설계하는 것이다. 최근 정부 간 조직으로 구축된 GEO(Group on Earth Observations)는 지구전반에 걸쳐 지속적으로 관측되는 자료를 활용하여 인류의 이익을 최대 창출하기 위해 노력하고 있다. 이러한 세계적 흐름에 따라 국내에서 관측되는 지구 관측자료 즉, 재해, 보건의료, 에너지자원, 기후, 수자원, 기상 생태계/생물다양성, 농업, 산림, 해양, 우주, 국토공간정보 등 12개 분야의 관측자료를 통합 및 활용하기 위한 인프라 구축이 반드시 필요하다. 현재 지구관측자료는 관측분야별로 개별적인 메타데이터를 관리하고 있을 뿐 메타데이터를 통합관리하지 못하고 있다. 국내 지구 관측자료의 관측분야와 관측요소별 다양하고 상이한 메타데이터로 인한 통합메타데이터의 설계 어려움을 메타-메타데이터를 이용하여 개발하였다.

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