N4SSB 단백질의 C-말단기의 7개의 아미노산이 N4SSB 단백질의 in vivo 활성에 미치는 영향

최미영,Choi, Mieyoung 한국미생물학회 1998 미생물학회지 Vol.34 No.4

Bacteriophage N4, a lytic phage specific for Esherichia coli K12 strain encodes single-stranded DNA-binding protein, N4SSB (bacteriophage N4-coded single-stranded DNA-binding protein). N4SSB protein is originally identified as a protein required for N4 DNA replication. N4SSB protein is also required for N4 late transcription, which is catalyzed by E. coli ${\sigma}^{70}$ RNA polymerase. N4 late transcription does not occur until N4SSB protein is synthesized. Recently it is reported that N4SSB protein is essential for N4 DNA recombination. Therefore N 4SSB protein is a multifunctional protein required for N4 DNA replication, late transcription, and N4 DNA recombination. In this study, a variety of mutant N4SSB proteins containing internal deletions or substitutions were constructed to define and characterize domains important for N4 DNA replication, late transcription, and N4 DNA recombination. Test for the ill vivo activity of these mutant N4SSBs for N4 DNA replication, late transcription, and N4 DNA recombination was examined. The results suggest that C-terminal 7 amino acid residues are important for the activity of N4SSB. Three lysine residues, which are contained in this region play important roles on N4SSB activity. Esherichia coli(E. coli) K12 균주를 숙주세포로 삼는 박테리오파아지인 N4는 single-stranded DNA에 결합하는 단백질인 N4SSB(bacteriophage N4-coded single-stranded DNA-binding protein) 단백질을 만든다. N4SSB 단백질은 N4 DNA replication 뿐만 아니라 late transcription과 N4 DNA recombination에도 필요한 여러 가지 기능을 가진 단백질이다. N4 late transcription은 숙주세포인 E. coli의 $E{\sigma}^{70}$ RNA polymerase에 의해서 수행이 되나 N4SSB 단백질을 반드시 필요로 하기 때문에 N4SSB 단백질이 생성될 때까지는 N4 late promoter로부터 RNA 합성이 일어나지 않는다. 본 연구에서는 N4SSB의 N4 DNA replication과 late transcription, 그리고 N4 DNA recombination에 필요한 영역(domain)을 알아내기 위해서 여러 가지 돌연변이형 N4SSB 단백질을 만들어 N4 DNA replication과 late transcription, 그리고 N4 DNA recombination의 3가지 작용에 대한 in vivo 활성을 조사 분석하였다. 그 결과 N4SSB 단백질의 C-말단기에 있는 7개의 아미노산이 N4SSB 단백질의 활성에 중요하다는 것을 알 수 있었다. 특히 C-말단기의 7개의 아미노산에는 세 개의 lysine이 포함되어 있는데 이 lysine이 N4SSB 단백질의 활성에 중요한 역할을 한다는 것이 제시되었다.

대구시 하수처리장 운전실태에 따른 중수도 도입 방안 연구

최미영,나욱호,홍원화,김삼열,Choi Mi-Young,Na Wook-Ho,Hong Won-Hwa,Kim Sam-Yeul 한국주거학회 2005 한국주거학회 논문집 Vol.16 No.1

It has seen the significant increase of water consumption rate due to rapid industrialization, high-densities of city, and increasing the population; thereby leads further water resource required in near future. In order to solve this deficit there are one of solution that gray water method demonstrates advantages regarding the questions of optimal water utility and thereby master plan of water. The method of study is analysis of investigation data through interview and research of sewage treatment plants in Daegu City. Through these analysis, this paper examines the capacity of graywater and optimal graywater facility.

기질약시에 의한 소아 감각사시의 임상 분석

최미영,황정민,Mi-Young Choi,Jeong-Min Hwang 대한안과학회 2005 대한안과학회지 Vol.46 No.8

Purpose: To study the clinical characteristics of pediatric sensory strabismus in Korean children. Methods: A retrospective analysis was performed on 71 patients with the diagnosis of sensory strabismus before the age of 16 years. Patients with strabismic amblyopia or anisometropic amblyopia were excluded from consideration. The age at onset of vision loss, diagnosis, type of strabismus, deviated angle, etiologic factors leading to vision loss, and visual acuity of the deviated eye were recorded. The surgical results were analyzed in the case of strabismus surgery for sensory strabismus. Results: The mean age at the onset of vision loss was 4.6 years and the mean age at diagnosis was 7.5 years. Forty-one patients (58%) had congenital vision loss and the most common cause of vision loss was optic nerve disease in 35 (49%). Exotropia developed in 58 (82%), and patients with severely impaired visual acuity were more likely to develop exotropia (P=0.054). The age at the onset of vision loss and the age at diagnosis in esotropia were younger than in exotropia (P=0.049, P=0.047, respectively). Twenty-five (78%) of 32 patients who had undergonewere strabismus surgery had ocular alignment within 10 prism diopters of orthophoria. The frequency of consecutive exotropia was 40% in surgery for esotropia. Conclusions: Children with organic amblyopia tend to develop sensory exotropia. We considered that the age at initial vision loss and the severity of vision loss could play a role in determining the direction of deviation. The surgical results were favorable, but we should pay attention to the development of consecutive exotropia in surgery for esotropia.

소아 눈모음부족외사시에서 근거리/원거리 사시각에 따른 단안 절제술 및 후전술

최미영,황정민.Mi-Young Choi. M.D.. Jeong-Min Hwang. M.D. 대한안과학회 2005 대한안과학회지 Vol.46 No.10

Purpose: The purpose of this study was to evaluate prospectively the long-term surgical results of unilateral lateral rectus (LR) muscle recession and medial rectus (MR) muscle resection in children with convergence insufficiency exotropia (XT). Methods: This prospective study included 14 children with an angle of XT greater at near than at distance by 10 prism diopters (PD) or more. The amounts of resection and recession were based on near and distance deviation, respectively. Minimum follow-up was one year (mean, 26.6±19.4 months) after surgery. Results: Significant postoperative reduction was achieved in mean distance exodeviation from 22.5 PD to 9.1 PD (p=0.000), and in mean near exodeviation from 33.8 PD to 13.6 PD (p=0.000). The mean near-distance difference decreased from 11.3 PD preoperatively to 4.6 PD postoperatively (p=0.000). A Fresnel prism was used temporarily to treat postoperative esotropia in only one patient for 6 months postoperatively. Conclusions: Unilateral surgery biased more to MR strengthening than to LR weakening in children with convergence insufficiency XT, was found to successfully reduce both distance and near deviation and to collapse near-distance differences with a low risk of long-term postoperative esotropia.

5-11세 소아에서 코로나19 백신의 효능 및 안전성에 대한 체계적 문헌고찰

최미영,유수연,정채림,최영준,최수한,Choi, Miyoung,Yu, Su-Yeon,Cheong, Chelim,Choe, Young June,Choi, Soo-Han 대한소아감염학회 2022 Pediatric Infection and Vaccine Vol.29 No.1

Purpose: To evaluate the efficacy and safety of coronavirus disease 2019 (COVID-19) vaccines in children aged 5-11 years, a rapid systematic review was conducted on published clinical trials of COVID-19 vaccines and studies that analyzed real-world data on adverse events after COVID-19 vaccination. Methods: A systematic search was conducted on medical literature in international (Ovid-MEDLINE) and pre-published literature databases (medRxiv), followed by handsearching up to January 4, 2022. We used terms including COVID-19, severe acute respiratory syndrome coronavirus 2, and vaccines, and the certainty of evidence was graded using the GRADE approach. Results: A total of 1,675 studies were identified, of which five were finally selected. Among the five studies, four consisted of data from clinical trials of each of the four types of COVID-19 vaccines (BNT162b2, mRNA-1273, CoronaVac, and BBIBP-CorV). The remaining study consisted of real-world data on the safety of the BNT162b2 vaccine in children aged 5-11 years. This systematic review identified that COVID-19 vaccines in recipients aged 5-11 years produced a favorable immune response, and were vaccines were effective against COVID-19. The safety findings for the BNT162b2 vaccine in children and early adolescents aged 5-11 years were similar to those data noted in the clinical trial. Conclusions: There is limited data on COVID-19 vaccines in children aged 5-11 years. Consequently continuous and comprehensive monitoring is necessary for the evaluation of the safety and effectiveness of the COVID-19 vaccines.

가미생간탕(加味生肝湯)이 고지방식이로 유발된 지방간에 미치는 영향

최미영,우홍정,김영철,이장훈,Choi, Mi-Young,Woo, Hong-Jung,Kim, Young-Chul,Lee, Jang-Hoon 대한한방내과학회 2009 大韓韓方內科學會誌 Vol.30 No.2

Objectives : The aim of this study was to investigate the effects of Gamisaenggan-tang on high fat diet induced nonalcoholic fatty liver disease. Methods: Rats were randomly divided into four groups. The Normal group was fed a solid diet containing 10% fat. The Gamisaenggan-tang (GS) group was fed a solid diet containing 10% fat and Gamisaenggan-tang (90mg/100g body weight). The Control group was fed a solid diet containing 60% fat. The HFD-Gamisaenggan-tang (HFD-GS) group was fed a solid diet containing 60% fat and Gamisaenggan-tang (90mg/100g body weight). Six weeks later, rats body weight, liver weight, serum ALT, GGT, ALP levels were measured. Histological findings (Oil red O staining), hepatic triglyceride, TNF-${\alpha}$, and TGF-${\beta}$ levels in the liver tissue were studied. Results: Average body weight of the HFD-GS group was significantly less than that of the Control group. There were no significant liver weight differences among each group. The GGT levels of the HFD-GS group were significantly less than those of the Control group. However, there were no significant differences in the ALT or ALP levels among the groups. TNF-${\alpha}$ protein production assessed by western blot analysis was reduced by Gamisaenggan-tang. Greater fat accumulation was observed in the liver tissue of the Control group than in the HFD-GS group, which means the Gamisaenggan-tang has an inhibitory effect on the accumulation of fat in the liver. Conclusion : The results suggest that Gamisaenggan-tang can be potential candidate for the treatment of nonalcoholic fatty liver disease in clinics.

한 눈의 시력이 0.1 이하인 순수 굴절부등약시의 임상 분석

최미영,김연희,Mi Young Choi,Youn Hui Kim 대한안과학회 2008 대한안과학회지 Vol.49 No.6

Purpose: To analyze the results of treatment in pure anisometrpic amblyopia with unilateral visual acuity of 0.1 or worse. Methods: The major eligibility criteria included pure anisometropic amblyopia with age <10 years, best-corrected visual acuity 0.1 or worse in the worse eye and 0.5 or better in the good eye after the wearing of optimal spectacle correction for a minimum of 4 weeks, and the minimum follow-up was 6 months. Twenty-one patients were selected and we analyzed the age at the amblyopia treatment, the type and degree of anisometropia, and the final visual acuity after treatment for visual improvement. Successful treatment was defined as a final visual acuity of 0.3 or more. Results: The mean age at treatment was 5.2 years. The types of anisometropia were hypermetropic in 14 patients, astigmatic in 4, and myopic in 3. The mean duration of treatment of amblyopia was 16 months, and 13 of 21 children with pure anisometropic amblyopia achieved 0.3 or better. The age at treatment was younger and the compliance rate was higher in the success group than in the failure group. Conclusions: The most common type was hyperopic in the pure anisomteropic amblyopia. Compliance and age at treatment were significantly related to best visual acuity attained in pure anisometropic amblyopia with unilateral visual acuity of 0.1 or worse. J Korean Ophthalmol Soc 49(6):973-978, 2008

요가와 필라테스 교양체육수업에 대한 질적 사례 연구

최미영 ( Choi Mi-young ) 한국체육학회 2020 한국체육학회지 Vol.59 No.3

본 연구의 목적은 요가와 필라테스 교양체육수업 참여자의 경험 의미와 그 가치를 이해하고자 한다. 이를 위해 D대학교에서 요가와 필라테스 수업을 수강한 학생 12명을 유목적 표본표출(Purposeful Sampling)에 따라 연구 참여자로 선정하였으며, 심층면담, 보고서, 참여관찰을 통해 자료를 수집하여 Stake(2006)가 제시한 방식에 따라 자료 분석한 결과는 다음과 같다. 첫째, ‘공간의 자유로움’과 ‘조별수행의 명암’을 포함한 ‘놀이와 경쟁의 혼재’가 도출되었으며, 둘째, ‘나도 인싸(Insider)구나’와 ‘일상의 전이(Transfer)’를 포함한 ‘외부와 연결되는 몰입(Flow)’의 양상이 발견되었다. 마지막으로 ‘통제가 안 되는 몸’과 ‘피드백과 직결되는 동기(Motivation)’를 포함한 ‘흔들리는 반응(Reaction)’이 확인되었다. 이러한 연구결과는 요가와 필라테스 교양체육수업 참여자들의 경험적 의미를 통해 교양체육수업의 교육적 함의와 그 의의를 발견하여 향후 교양체육수업의 발전적인 방안에 내실을 다지는 시스템과 프로그램, 교수법을 개발하는데 유용한 자료를 제공하고자 한다. The purpose of this study is to understand into the meaning of experience of participants in yoga and pilates general physical education classes and its value. For this purpose, 12 students who attended yoga and pilates classes at D University were selected as study participants based on Purposeful Sampling. The results of data analysis according to the method suggested by Stake (2006) by collecting data through in-depth interviews, reports, and observations are as follows. Firstly, ‘confusion of play and competition’, including ‘freedom of space’ and ‘light and darkness of group performance’ were derived. Secondly, the aspects of ‘I am an Insider, too’ including ‘daily transfer’ and ‘flow connected to the outside’ were revealed. Lastly, “wobbling reaction” and ‘motivation directly related to feedback’ including ‘uncontrollable body’ were confirmed. These findings are useful for developing systems, programs, and teaching methods to discover the educational implications and significance in the general physical education through the empirical meaning of participants in yoga and pilates general physical education classes.

응급구조학과 학생들의 비판적 사고성향이 임상실습만족도와 추천의도에 미치는 영향

최미영(Choi, Mie-Young),문태영(Moon, Tae-Young),이현지(Lee, Hyun-Ji) 한국산학기술학회 2013 한국산학기술학회논문지 Vol.14 No.3

본 논문은 병원임상실습관련 응급구조학과 학생들의 비판적 사고성향이 임상실습만족도와 추천의도에 나타 나는 관계를 규명하는데 있다. 자료 분석을 위하여 사용된 통계방법은 Windows용 SPSS 20.0과 AMOS 20.0을 사용 하였으며, 빈도분석, 문항내적 일치도를 측정하는 Cronbach's ⍺, 요인분석, 다중회귀분석, 구조방정식 모형 분석 등 을 실시하였다. 모든 통계분석은 유의수준 .05에서 분석하였다. 자료 수집은 2012년 5월 12일부터 8월 25일까지 조 사를 실시하여 305명을 수집된 자료를 활용하였다. 응급구조학과 학생들의 비판적 사고성향은 임상실습 만족도에 유 의한 영향을 주는 것으로 나타나 채택되었고, 비판적 사고성향이 추천의도에 유의한 영향을 주는 것으로 나타나 채 택되었으며, 임상실습 만족도는 추천의도에 유의한 영향을 주는 것으로 나타나 채택되었다. 이러한 연구 결과로 볼 때 질적으로 우수한 응급실 응급구조사의 전문 인력이 되기 위해서는 응급구조학과 학생들의 임상실습을 통해 필요 한 교육 내용과 프로그램 개발의 질적 관리가 체계적이고 지속적으로 이루어져야 할 것으로 사료된다 The purpose of this study was to examine the relationship of the critical thinking disposition of paramedic students to their satisfaction with hospital clinical practice and intention of recommendation. As for data analysis, the statistical packages SPSS WIN 20.0 and AMOS 20.0 were employed, and frequency analysis, factor analysis, multiple regression analysis and structural equation modeling were utilized. Besides, Cronbach alpha coefficients were calculated to check item internal consistency. The level of significance was all set at .05. A survey was conducted to collect data from May 12 to August 25, 2012, and the answer sheets from 305 respondents were gathered and analyzed. The critical thinking disposition of the paramedic students was found to have a significant impact on their satisfaction with clinical practice, and the hypothesis about that was accepted. Their critical thinking disposition was found to exert a significant influence on their intention of recommendation as well, and the hypothesis about that was accepted. Finally, their satisfaction with clinical practice was found to affect their intention of recommendation in a significant way, and the hypothesis about that was accepted. The findings of the study suggested that in order to nurture professional and competent paramedics, the content of clinical practice should systematically be managed on a regular basis, and prolonged research efforts should be directed into program development.

국어 수업을 통한 창조적 미래 인재 육성 방안 연구

최미영 ( Choi Mi-young ) 창조교육학회 2022 창조교육논총 Vol.24 No.-

For our students, middle school is the most important time of their lives. I believe that our students' future will be more meaningful and happy lives depending on what values they establish and continue to practice during this period. While conducting class conversations, emotional expression writing classes, reading discussion classes using textbooks, and convergence project classes, I saw students' lively class expressions, and I thought again that our teachers' mission is to lead and help our students create a happy life. Through the Korean language class conversation process, I wanted to present directions like a systematic and structured way of life to future talented students and guide them to be familiar with their minds and bodies. In other words, I constantly thought about how to foster creative future talent through Korean classes in the hope that it will create its own meaning in classes with students that are repeated every day and help them enter a bigger society and live as confident people in the future. Our students are the ones responsible for our future era. It is an important role of teachers to lead the direction of how to live for our students, who are future talents. There are so many words that define future talent. I think it is also an unreasonable expression to define the future talent in a few words. However, I believe that if children who have not yet established systematic thinking and direction are given specific words, it will help them grow through direction setting and practice. Writing of emotional expression for conversation and aesthetic enhancement in Korean class. In reading discussions using textbook works and curriculum convergence project classes, consistent classes and school life guidance were conducted to set future talents with personality, problem-solving skills, and creativity, and to continuously present their justification and direction. The intention was to create a healthy personality that smells like a person who benefits humans in a society where they live with others, a person who has the ability to solve any problem on his own, and a creative person who can make a life based on fresh and creative ideas about what he or she does in an anxious society. To that end, efforts were also made to connect with students' healthy lives by creating a practical stage that can continuously structure, internalize, and embody class conversations. It is class conversation, structuring, internalization, and physicalization, which are the four stages of class conversation.