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β-Asarone이 Lipopolysaccharide에 의한 생쥐 해마의 염증성 사이토카인 발현과 학습 및 기억 장애에 미치는 영향
최문숙 ( Moon Sook Choi ),곽희준 ( Hee Jun Kwak ),권기중 ( Ki Jung Kweon ),황지모 ( Ji Mo Hwang ),신정원 ( Jung Won Shin ),손낙원 ( Nak Won Sohn ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.6
Objectives : β-Asarone (BAS) is an active ingredient in Acori Rhizoma. This study investigated anti-neuroinflammatory and memory ameliorating effects of BAS in systemic lipopolysaccharide (LPS)-treated C57BL/6 mice. Methods : BAS was administered orally at doses of 7.5, 15, and 30 mg/kg for 3 days prior to LPS (3 mg/kg, intraperitoneal) injection. Pro-inflammatory cytokine mRNA, including tumor necrosis factor-α (TNF-ㅍ), interleukin (IL)-1β and IL-6, was measured in hippocampus tissue using real-time polymerase chain reaction at 4 h after the LPS injection. An ameliorating effect of 30 mg/kg BAS on learning and memory impairment in the LPS-treated mice was verified using the Morris water maze test. Results : BAS significantly attenuated up-regulation of TNF-α, IL-1β, and IL-6 mRNA in hippocampus tissue of the LPS-treated mice. In acquisition training test, BAS improved learning performance of the LPS-treated mice with a significant decrease of escape latency to the platform. In memory retention test, BAS also ameliorated memory impairment of the LPS-treated mice with a significant increase of swimming time in zones neighboring to the platform, number of target heading, and memory score. Conclusion : The results suggest that inhibition of pro-inflammatory cytokines and neuroinflammation in the hippocampus by BAS could be one of the mechanisms for BAS-mediated ameliorating effect on learning and memory impairment in LPS-treated mice.
Generation of donor-derived rainbow trout by transplantation of frozen-thawed ovarian germ cells
이승기,최문숙,배창환,여주홍 한국발생생물학회 2017 한국발생생물학회 학술발표대회 Vol.2017 No.8
Cryopreservation is an effective method for the long-term storage of fish sperm. However, because of a lack of methods for cryopreserving fish eggs and embryos, a technical challenge remains in preserving maternally inherited cytoplasmic compartments. Here, we aimed to generate functional eggs and sperm in sterile rainbow trout hatchlings by transplanting cryopreserved ovarian germ cells into the peritoneal cavity. Immature ovaries were isolated from 9-month-old transgenic rainbow trout, whose ovarian germ cells expressed green fluorescent protein (GFP), and cryopreservation was optimized by testing several different cryoprotective agents. Dominant orange-colored, pvasa-Gfp transgenic rainbow trout females served as donors, and wild-type triploid rainbow trout served as germ cell recipients. Viability and transplantation efficiencies of frozen ovarian germ cells were stable for up to 1,185 days. Ovarian germ cells frozen for 8months efficiently differentiated into eggs and sperm in the ovaries and testes of recipient fish. Inseminating the resultant eggs and sperm generated viable offspring displaying the donor phenotypes (orange body color, green fluorescence, XX ovaries, and matching karyotype). This study represents the first success in generating functional gametes from cryopreserved female germ cells in any fish species, which should facilitate the conservation of protogynous fishes, female heterogametic fish, and currently endangered fish.
김홍근,박관호,이석현,곽규원,최문숙,최지영 한국잠사학회 2015 International Journal of Industrial Entomology Vol.31 No.1
The white-spotted chafer, Protaetia brevitarsis (Coleoptera: Scarabaeidae), has been traditionally used in Korea as a medicine for preventing liver-related diseases and suppressing liver cancer. Therefore, this insect is economically important and is commercially reared and sold in Korea. Recently, P. brevitarsis was listed as a temporal food ingredient by the Korean Ministry of Food and Drug Safety. Given the increasing economic importance of this beetle, we have sought to improve rearing conditions for its commercial production. In this study, we compared the effects of two food supplements, clay and charcoal, on the growth of second instar larvae of P. brevitarsis. Clay and charcoal are generally known as good adsorbent for removal of contaminating substances in insect feed. We fed second instar. brevitarsis larvae a commercial diet consisting of fermented sawdust with seven different combinations of clay and/or activated charcoal, and measured their effects on weight gain for approximately 17 wk until larvae pupated. We found that addition of clay at 2.5% w/w of the fermented sawdust diet had no negative effect on weight gain of second instar P. brevitarsis larvae and thus may improve the quality of P. brevitarsis as a commercial food