외식산업의 판매촉진 전략이 고객만족과 재방문 의도에 미치는 영향

최동국(Choi, Dong-Kug) 한국외식산업경영학회 2019 외식산업경영연구 Vol.15 No.1

The purpose of this study is to analyze the correlation between the sales promotion strategy of the dining industry and customer satisfaction and intention to return to the premise in order to overcome the recession in the industry. It is to provide the industry with useful data for the establishment of effective sales promotion strategy and to contribute to sales of the companies and food service providers in the industry. From the results of the study, the followings were found. First, limited offer, unlimited replenishment, and price event made significant effects on custsomer satisfaction, but 1+1 event did not make significant effects on the customer satisfaction. Second, all sales promotion strategies are found to make significant effects on the intention to visit the premise again. Third, the relationship between customer satisfaction and intention to visit again was the relationship of positive and significant effects. Through the above research results, it is concluded that the sales promotion strategies serving as escapes to survive in the market of severe recession can satisfy various customers’ requirements by season, by time, by price, and by age and can lead them to visit the premises again. It is expected that effectively tailored marketing strategies which suit for the situation will bring bigger effects.

외식업체의 로스리더 마케팅 요인과 브랜드 태도, 재방문 의도 관계

최동국(Dong-Kug Choi) 한국조리학회 2017 한국조리학회지 Vol.23 No.7

대학의 외식창업교육 서비스품질이 학생만족도와 창업의지에 미치는 영향: 학교기업 프로그램 참여자들을 중심으로

최동국(Choi, Dong-Kug) 한국외식산업경영학회 2020 외식산업경영연구 Vol.16 No.1

This study analyzes the correlation between college food service entrepreneurship education, student satisfaction, self-efficacy, and entrepreneurial intention. Major results of the study are as follows. First, Out of five sub-factors of service quality (SERVQUAL) of foodservice start-up education, the factors of materiality, reliability, and empathy were verified to have a significant effect on student satisfaction, while responsiveness and certainty did not make significant effects on student satisfaction. Second, students" satisfaction with foodservice entrepreneurship education did not directly affect the will of entrepreneurial intention of the students. It rather indirectly affects through self-efficacy. Third, student satisfaction was found to have a positive effect on self-efficacy. Fourth, self-efficacy was found to have a positive effect on entrepreneurial intention. Fifth, self-efficacy was found to play a full mediating role in the relationship between student satisfaction and entrepreneurship. This study is meaningful in that it presented basic data for future start-up research as a concrete empirical analysis on the effect of the service quality of college foodservice entrepreneurship education on student satisfaction and entrepreneurial intention. In particular, this study can be differentiated from other existing studies in that it found that the positive effect of self-efficacy is a variable that enhances the entrepreneurial intention through educational satisfaction.

LHCP gene 의 발현을 조절하는 인자의 규명 및 동정

이화형(Wha Hyung Lee),박희진(Hee Jin Park),최동국(Dong Kug Choi),김인수(In Soo Kim),박진우(Jin Woo Park) 경북대학교 생명공학연구소 1991 遺傳工學硏究所報 Vol.6 No.1

N/A

왕대, 조릿대, 오죽의 추출 용매에 따른 항산화, 신경염증제어 활성 및 지표성분 caffeic acid, p-coumaric acid, tricin의 함량 비교

김연숙(Yon-Suk Kim),조덕연(Duk-Yeon Cho),김미경(Mikyung Kim),최동국(Dong-Kug Choi) 한국식품과학회 2021 한국식품과학회지 Vol.53 No.3

본 연구에서는 국내에 자생하는 대나무 중 3종(왕대, 조릿대, 오죽)의 죽엽을 4종의 추출용매(물, 30% 주정, 70% 주정, 100% 주정)를 이용하여 추출 후 추출물별 항산화 활성 탐색, 신경염증 억제 효과 및 HPLC-UV 분석 방법에 의한 3가지 지표성분(caffeic acid, p-coumaric acid 및 Tricin)의 함량을 측정하였다. FRAP 방법과 DPPH 라디칼 및 ABTS 라디칼 소거능 측정방법을 이용하여 항산화 활성을 탐색하였으며, 3종의 대나무 중에서는 오죽 추출물이 총폴리페놀 및 총플라보노이드 함량이 가장 높았다. FRAP 방법, DPPH 라디칼 및 ABTS 라디칼 소거능 방법을 이용하여 항산화 활성을 측정한 결과 또한 오죽 추출물이 가장 우수하였다. Caffeic acid의 함량은 오죽의 물추출물이 6.247±0.049 μg/mg으로 가장 높았으며, p-coumaric acid의 함량은 오죽의 70% 주정추출물이 10.931±1.467 μg/mg으로 가장 높았으며 다음으로는 오죽의 100% 주정추출물이 9.374±1.140 μg/mg으로 높게 나타났으며 3종 죽엽의 물추출물을 제외한 주정추출물은 모두 p-coumaric acid 함량이 높음을 확인하였다. Tricin의 함량을 측정한 결과 또한 물추출물에서는 함량이 낮았으나, 70% 주정추출물이나 100% 주정추출물은 3종의 죽엽추출물 모두에서 3.094±0.875-5.233±2.698 μg/mg로 비슷한 함량을 나타내었다. LPS를 사용하여 미세아교세포의 활성화를 유도하여 신경염증반응의 지표인 NO의 생성량을 측정한 결과 3종의 죽엽추출물(70% 주정추출물) 모두 농도 의존적으로 NO의 생성량을 억제하였다. 따라서 본 연구의 결과는 죽엽추출물이 미세아교세포의 과도한 신경염증 활성화로 인해 발생되는 뇌 신경질환의 치료 소재로서 활용 가능성을 제시하였다. The antioxidant and anti-neuroinflammatory activities of water, 30, 70, and 100% ethanol extracts of leaves of three different species of bamboo (Phyllostachys nigra, P. bambusoides, and Sasa borealis) were investigated. The levels of total polyphenol and flavonoid were measured, and antioxidant activity was evaluated using various antioxidant assays (DPPH, ABTS, and FRAP). Lipopolysaccharide (LPS)-induced BV2 microglial cell activation was used to evaluate the anti-neuroinflammatory properties of the bamboo leaf extracts. Treatment with both aqueous and ethanolic extracts showed no cytotoxicity in BV-2 microglial cells. Pre-treatment of BV-2 cells with bamboo leaf extracts significantly inhibited LPS-induced excessive production of nitric oxide in a dose-dependent manner. Moreover, phytochemical analysis based on the extraction solvent showed that caffeic acid, p-coumaric acid, and tricin are the principal constituents of all three bamboo leaf extracts. Therefore, our findings suggest that bamboo leaf extract contains potent antioxidants and anti-neuroinflammatory compounds that can be used as potential therapeutic agents for the treat neuroinflammatory diseases.

Gastrodia elata Blume extract inhibits the production of nitric oxide and proinflammatory mediators in LPS-stimulated BV-2 microglia cells via JNK mitogen-activated protein kinase and NF-kappa B singnaling pathway

Byung-Wook Kim,Sushruta Koppula,Sun-Min Hong,Hyung-Woo Lim,Dong-Kug Choi(최동국) 한국약용작물학회 2012 한국약용작물학회 학술대회논문집 Vol.2012 No.1

High Resolution Genomic Profile of Neuro2a Murine Neuroblastoma Cell Line by Array-based Comparative Genomic Hybridization

Jin Hwan Do(도진환),In Su Kim(김인수),Hyun Myung Ko(고현명),Dong-Kug Choi(최동국) 한국생명과학회 2009 생명과학회지 Vol.19 No.4

신경아세포종은 미분화된 신경외배엽 세포로부터 유래한 신경능세포에 의해 형성된 소아기에 보는 가장 많이 발생하는 악성 종양 중 하나이다. 신경아세포종인 Neuro-2a 세포는 신경세포의 분화, 세포사 억제 효능, 세포독성 검정 등에 활용되고 있다. Neuro-2a 역시 다른 신경아세종과 같이 염색체 변이를 가지고 있지만, 이에 대해 고밀도의 게놈수준에서 염색체 변이에 대해 보고된 바가 없다. 본 연구에서는 고집적 마이크로어레이(최소 43,000 개의 코딩, non-코딩 유전자 서열이 집적된 마이크로어레이)기반의 비교유전체보합법을 활용하여, 고해상도의 Neuro-2a 유전체 이상을 분석하였다. 마이크로 어레이 데이터는 Hidden Markov Model을 활용하여, 유전체 변이를 double loss, single loss, normal, single gain 그리고 amplification으로 나누어 분석하였다. Neuro2a는 MYCN 유전자의 증폭은 관찰되지 않았고, GDNF, BDNF, NENF등의 neurotrophic factor 가운데 NENF의 gain 현상이 관찰 되었다. 염색체의 이상은 4,8,10,11,15번에서 발견되었으며, 염색체 3,17,18,19에서는 전부 20개 미만의 염색체 이상이 발견되었다. 염색체 이상이 연속적으로 일어난 부위 중 gain으로서 가장 긴 부분은 Chr8:8,427,841-35,162,415의 약 26.7 Mb이며, single loss로서 가장 긴 곳은 Chr4:73,265,785-88,374,165의 약 15.1 Mb였다. 염색체의 위치는 UCSC 데이터베이스(UCSC ㎜8, NCBI Build 36)에 근거하였다. Murine Neuro-2a (N2a) cells have been widely used for the investigation of neuronal differentiation, trophic interaction and neurotoxic effects of various compounds and their associated mechanisms. N2a cells have many genomic variations such as gains or losses in DNA copy number, similar to other neuroblastoma cells, and no systematic or high-resolution studies of their genome-wide chromosomal aberrations have been reported. Presently, we conducted a systematic genome-wide determination of chromosomal aberrations in N2a cells using a high-throughput, oligonucleotide array-based comparative genomic hybridization (oaCGH) technique. A hidden Markov Model was employed to assign each genomic oligonucleotide to a DNA copy number state: double loss, single loss, normal, gain, double gain and amplification. Unlike most neuroblastoma cells, Mycn amplification was not observed in N2a cells. In addition, these cells showed gain only in the neuron-derived neurotrophic factor (NF), while other neurotrophic factors such as glial line-derived NF and brain-derived NF presented normal copy numbers. Chromosomes 4, 8, 10, 11 and 15 displayed more than 1000 aberrational oligonucleotides, while chromosomes 3, 17, 18 and 19 displayed less than 20. The largest region of gain was located on chromosome 8 and its size was no less than 26.7 Mb (Chr8:8427841-35162415), while chromosome 4 had the longest region of single deletion, with a size of 15.1 Mb (Chr4:73265785-88374165).

Arabidopsis 의 cab Promoter 의 광조절부위 및 그에 결합하는 단백질 인자의 동정

박희진 ( Hee Jin Park ),김인수 ( In Soo Kim ),이화형 ( Hwa Hyung Lee ),최동국 ( Dong Kug Choi ) 경북대학교 생명공학연구소 1993 遺傳工學硏究所報 Vol.8 No.1

N/A

위축 위염과 장형화생에서의 마이크로어레이를 이용한 유전자 발현

김경래 ( Kyong Rae Kim ),오수연 ( Soo Youn Oh ),박웅채 ( Ung Chae Park ),왕준호 ( Joon Ho Wang ),이재동 ( Jae Dong Lee ),권혁중 ( Hyuk Jung Kweon ),김상윤 ( Sang Yoon Kim ),박승화 ( Seung Hwa Park ),최동국 ( Dong Kug Choi ),김찬길 대한소화기학회 2007 대한소화기학회지 Vol.49 No.4

목적: 장형화생을 동반한 위축 위염은 최근까지 위암발생의 중요한 요인으로 여겨져 왔다. 그러나 아직 장형화생과 위축 위염에 발현하는 주요 유전자에 관해서 거의 알려진 것이 없다. 이번 연구는 기존의 유전자 탐색에 사용되었던 어떤 방법보다도 많은 장점을 가지고 있는 올리고뉴클레오티드 마이크로어레이를 이용하여 전반적인 유전자 변화와 함께 위암과의 관련성을 알아보고자 하였다. 대상 및 방법: 장형화생을 동반한 위축 위염환자의 위점막과 정상 점막에서 올리고뉴클레오티드 마이크로어레이 기술을 이용하여 대량의 유전자 정보를 얻었으며, 유전자들의 확인을 위해 SAM (Significance Analysis of Microarrays) package 방법을 사용하였다. Global normalization, intensity dependent normalization 그리고 box plot normalization를 사용하여 결과를 분석하였다. 결과: FABP, REG, OR6C1, MEP1, SLC6A1, SI, Mucin 1, RAB23 등 8개 유전자는 정상점막에 비해 10배이상 과발현되었고, LOC44119유전자 하나만이 10배 이상 저발현되었다. 알려진 위암의 발암과정에 관여된 유전자로는 FN1, SRMS, TP53, TP53IMP2, TP53I3, FGFR4, TGFB1, TGFA 등 모두 8개에서 공통적으로 2배 이상의 발현 증감을 보였다. 결론: 저자들은 위암의 전단계병변인 장형화생을 동반한 위축 위염에서 암화와 관련한 유전자를 확인할 수 있었으며, 이번 연구 결과는 위암 진단 및 치료와 같은 임상적용을 위한 분자생물학적인 특성을 이해하는 데에 도움이 될 것이다. Background/Aims: The atrophic gastritis with intestinal metaplasia of gastric mucosa has been considered to be the major factor of carcinogenesis in the stomach. However, the key molecules are still poorly understood. To elucidate the molecular genetic basis, we report the results of our initial microarray data to analyze the genome pattern in patients with atrophic gastritis and intestinal metaplasia of the stomach. Methods: We used oligonucleotide microarray technique to evaluate the gene expression profiles in atrophic gastritis with intestinal metaplasia, in comparison with those of normal mucosa. For the identification of differentially expressed genes, Significance Analysis of Microarrays (SAM) package method was used. The results were analyzed using global normalization, intensity dependent normalization, and box plot normalization. Results: Eight genes including FABP, REG, OR6C1, MEP1, SLC6A1, SI, Mucin 1, and RAB23 in mucosa of atrophic gastritis and intestinal metaplasia were up-regulated by more than 10 times as compared with normal gastric mucosa. Only one gene, LOC44119 was down-regulated by more than 10 times of the expression as compared with normal gastric mucosa. In respect to the expression of known genes related to gastric carcinogenesis, 8 genes including FN1, SRMS, TP53, TP53IMP2, TP53I3, FGFR4, TGFB1, and TGFA showed up- and down-regulations more than 2 folds in expression pattern. Conclusions: We could identify a total genome pattern in patient with atrophic gastritis and intestinal metaplasia using oligonucleotide microarray. We believe that the current results will serve as a fundamental bioinformative basis for clinical applications in diagnosis and treatment of gastric cancer and precancerous lesion in the future. (Korean J Gastroenterol 2007;49:209-224)

Arabidopsis 의 cab Promoter 의 광조절부위 및 그에 결합하는 단백질 인자의 동정

김인수,이화형,최동국 경북대학교 유전공학연구소 1992 遺傳工學硏究所報 Vol.7 No.1

Protein binding factors (light-regulated factor, LRF) and light-regulatory element (LRE) of Arabidopsis thaliana cab promoters have been studied. The mobility shift assay of several fragments of three cab promoters (AB140, AB165 and AB180) with nuclear proteins showed several retardation bands. Specificity of the band was proved by competition assays. To identify LRF band from the retardation bands, coinsidence of transcription activity and retardation band appearance has been studied with light/dark-treated samples. Footprinting analysis has also been tried to identify the sequence of LRE binding to LRF.