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뿌리혹 특이 단백질 Nodule - 24 의 담배에서의 발현
이석찬,피재호,윤은경,구영연,천종윤,천충일 한국유전학회 2000 Genes & Genomics Vol.22 No.3
Nodulin-24 is a soybean nodulin that is expressed specifically in root nodules. It was shown to have a cleavable signal sequence and localized to a symbiosome membrane. For understanding the targeting process of nodulin-24 to the symbiosome, two different sizes of nodulin-24 cDNAs were fused to β-glucuronidase (GUS) gene and the fused constructs were introduced into tobacco plant. The presence of the fusions in transgenic plants was confirmed by polymerase chain reaction (PCR). Histochemical staining of mature tobacco leaves with X-glucuronide did not show the expression of the nodulin-24/GUS fusions. However, their expression was found in apical meristems and roots of the transgenic tobacco plants. Northern analysis of mature leaves showed the presence of transcripts for the fusions. These results indicate that the fusions were expressed, and the products may be targeted to vacuole and degraded in mature leaves, but that they were not degraded in the maturing tissues.
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염희연,박준수,정동준,김창진,김용배,이대훈,김경중,천종윤,강천,정윤석,정향민 대한소아청소년과학회 2006 Clinical and Experimental Pediatrics (CEP) Vol.49 No.4
Purpose : Human metapneumovirus(hMPV) is a respiratory viral pathogen that causes a wide spectrum of illnesses, ranging from asymptomatic infection to severe bronchiolitis. The virus has been identified world widely, but so far it has not been published in Korea. Methods : We obtained clinical samples by nasopharyngeal aspiration from 218 children hospitalized due to acute lower respiratory tract infections at Soonchunhyang University Hospital in Cheonan from October, 2004 to April, 2005. We designed specific primers from conserved region of fusion glycoprotein of hMPV. Total RNA was extracted and RT-PCR was performed, and single specific 423 bp product was obtained. The PCR product was confirmed to be fusion glycoprotein RNA by sequencing. Results : We detected hMPV in 15(6.9 percent) of the 218 hospitalized children. The infected children comprised nine boys and six girls; their mean age was 2.8 years(5 mo-12 yrs) and they were diagnosed with pneumonia(60 percent), bronchiolitis(33.3 percent), croup(6.6 percent). The number of cases of detected hMPV in Korea increased dramatically during the period from March to May 2005. Conclusion : hMPV is circulating in Korean children and is associated with respiratory tract infection. Additional studies are required to define the epidemiology and the extent of diseases in the general population caused by hMPV. 목 적 : 호흡기 감염증의 주된 원인 중 하나인 hMPV가 2001년 van den Hoogen 등에 의해 네덜란드에서 처음으로 발견된 후 여러 나라에서 보고되었지만, 국내에서 hMPV의 검출에 대한 보고는 대단히 드물다. 따라서 저자들은 우리나라에서 hMPV의 역학을 연구하고자 하였다.방 법 : 2004년 10월부터 2005년 5월까지 순천향대학교 천안병원 소아과에 급성 하기도염으로 입원한 218명의 비인두 흡인액을 추출한 뒤 RT-PCR을 시행하고, hMPV가 검출된 환아들의 비인두 흡인물에서 nucleotide의 sequencing을 하여 네덜란드에서 보고된 예와 비교하고, phylogenetic analysis를 하여 검출된 hMPV들간에 유사성을 검토하며, 배양검사로 hMPV의 검출됨을 재확인한 뒤, hPMV가 검출된 15명의 의무기록지를 토대로 이들의 임상 양상을 조사하였다.결 과 : 218명 중 15명의 비인두 흡인물에서 RT-PCR로 hMPV를 검출할 수 있었다(검출률 6.9%). hMPV가 검출된 15명의 비인두 흡인물 중 11명의 것으로 hMPV의 Fushion protein의 F gene에 대한 nucleotide sequencing을 하여 네덜란드의 것과 유사한 sequence임을 확인할 수 있었다. Phylogenetic analysis를 하여 본 연구에서 검출된 11개의 F gene이 두 개의 군으로 나뉘는 것을 알 수 있었다. hMPV 감염 환아의 평균연령은 2세 8개월(5개월-12세 2개월)이었고, 남녀비는 1.5 : 1이었고, 이들의 진단명은 폐렴(60.0%)이었고 모세기관지염(33.3%), 후두염(6.6%) 순으로 나타났다. 3월과 4월에만 집중적으로 검출되었고, 동시감염은 단 한 례에서 있었다. hMPV 감염 환아에서 기침이 100.0%, 콧물이 86.6%, 발열 80.0%, 호흡곤란 26.6%, 구토가 13.3%, 인후통와 설사와 두통이 각각 6.6%에서 나타났고, 발열 지속기간은 평균 4.9일(1-8일)이었으며, 입원기간은 평균 6.2일(3-14일)이었다. 신체 진찰상 나음이 80.0%에서, 호기성 천명음이 33.3%에서 청진되었고, 흉부 방사선 검사에서 33.3%에서 폐침윤이 관찰되었다. 검사실 소견상 말초혈액에서 중성구 감소증과 림프구 감소증이 각각 6.6%, 13.3%에서 나타났고, C 반응단백은 평균 19.3 mg/L(3.1-103)이었다.
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허윤선,신기혜,김성한,남경희,이명석,천종윤,천충일 한국분자세포생물학회 2009 Molecules and cells Vol.27 No.2
Development of symbiotic root nodules in legumes involves the induction and repression of numerous genes in conjunction with changes in the level of phytohormones. We have isolated several genes that exhibit differential expression patterns during the development of soybean nodules. One of such genes, which were repressed in mature nodules, was identified as a putative aldo/keto reductase and thus named Glycine max aldo/keto reductase 1 (GmAKR1). GmAKR1 appears to be a close relative of a yeast aldo/keto reductase YakC whose in vivo substrate has not been identified yet. The expression of GmAKR1 in soybean showed a root-specific expression pattern and inducibility by a synthetic auxin analogue 2,4-D, which appeared to be corroborated by presence of the root-specific element and the stress-response element in the promoter region. In addition, constitutive overexpression of GmAKR1 in transgenic soybean hairy roots inhibited nodule development, which suggests that it plays a negative role in the regulation of nodule development. One of the Arabidopsis orthologues of GmAKR1 is the ARF-GAP domain 2 protein, which is a potential negative regulator of vesicle trafficking; therefore GmAKR1 may have a similar function in the roots and nodules of legume plants.
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