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건조방법과 저장조건이 새싹보리의 대장균 저감과 품질 특성에 미치는 영향
최송이,채효빈,황인준,현정은,서우덕,김세리 한국식품저장유통학회 2022 한국식품저장유통학회지 Vol.29 No.7
Due to their high nutritional value and functional content, barley sprouts are commonly ground into powder for human use. This study investigated the effect of drying methods, viz., hot-air drying (HAD), freeze-drying (FD), and infrared drying (IRD), and storage conditions for the removal of Escherichia coli quality parameters of barley sprouts. Herein, the barley sprouts were subjected to HAD, FD, and IRD and stored at different temperatures, such as -20, 4, and 25℃, for six months. The changes in the colony count of E. coli and food quality indicators, viz., Hunter color, chlorophyll, and saponarin, were measured once a month. The HAD and FD were found to reduce E. coli from 7 log CFU/g to 4 log CFU/g, whereas IRD eliminated them (limit of detection < 1 log CFU/g). In the case of HAD, the E. coli counts and the quality of dried leaves did not change significantly (p>0.05) for six months under all storage conditions. Freeze-dried leaves stored at 25 and 4℃ showed a decrease in E. coli counts from 4.5 to 1.2-1.5 log CFU/g after six months (p<0.05), and no significant change in quality during their storage was observed (p>0.05). In the case of infrared-dried samples, E. coli was not detected even after six months under all storage conditions, storing at -20℃ was the best condition for the chlorophyll content and Hunter color (p<0.05). On the other hand, the content of saponarin was constant regardless of the drying method and storage.
전라북도 지역 농업용수의 미생물학적 특성 및 온도와 수질에 따른 농업용수의 병원성대장균 O157:H7 밀도 변화
황인준,함현희,박대수,채효빈,김세리,김황용,김현주,김원일 한국환경농학회 2019 한국환경농학회지 Vol.38 No.4
BACKGROUND: Agricultural water is known to be one of the major routes in bacterial contamination of fresh vegetable. However, there is a lack of fundamental data on the microbial safety of agricultural water in Korea. METHODS AND RESULTS: We investigated the density of indicator bacteria in the surface water samples from 31 sites collected in April, July, and October 2018, while the groundwater samples were collected from 20 sites within Jeollabuk-do in April and July 2018. In surface water, the mean density of coliform, fecal coliform, and Escherichia coli was 2.7±0.55, 1.9±0.71, and 1.4±0.58 log CFU/100 mL, respectively, showing the highest bacterial density in July. For groundwater, the mean density of coliform, fecal coliform, and E. coli was 1.9±0.58, 1.4±0.37, and 1.0±0.33 log CFU/ 100mL, respectively, showing no significant difference between sampling time. The survival of E. coli O157:H7 were prolonged in water with higher organic matter contents such as total nitrogen (TN), and nitrate-nitrogen (NO3-N). The reduction rates of E. coli O157:H7 in the water showed greater in order of 25, 35, 5, and 15℃. CONCLUSION: These results can be utilized as fundamental data for prediction the microbiological contamination of agricultural water and the development of microbial prevention technology.
전처리 농산물의 유통 경로에 따른 미생물학적 오염도 조사
현정은,이수빈,정도영,채효빈,최송이,황인준,김세리 한국식품영양과학회 2023 한국식품영양과학회지 Vol.52 No.3
This study investigates microbial contamination in pre-treated agricultural products. Eleven types of pre-treated agricultural product were evaluated for microbial contamination arising from four distribution channels: supermarket, local market, online, and traditional market. Of the 38 samples examined, the average populations of total aerobic bacteria and coliform in sliced garlic were higher by 6.85 and 5.94 log CFU/g, respectively. Similarly, the average populations of total aerobic bacteria and coliform determined in shredded green onion were 6.39 and 5.46 log CFU/g, respectively. In particular, the average populations of total aerobic bacteria were higher in sliced garlic and shredded green onion than in peeled garlic and peeled green onion. Furthermore, Bacillus cereus (13.16%) was detected in peeled chestnut, peeled potato, cut Chinese yam, and cut green onion. In addition, Staphylococcus aureus was isolated from cut radish in 1 out of 38 samples (2.63%). However, no correlation was obtained between microbial population and distribution channel in all samples. These results could provide the basic information to control the microbial safety of pre-treated agricultural products.
경기, 강원 지역 농업용수의 미생물학적 특성 및 농업용수 분리 대장균의 항생제 내성
황인준 ( Injun Hwang ),박대수 ( Daesoo Park ),채효빈 ( Hyobeen Chae ),김은선 ( Eunsun Kim ),윤재현 ( Jae-hyun Yoon ),나겐드란라자린감 ( Nagendran Rajalingam ),최송이 ( Songyi Choi ),김세리 ( Se-ri Kim ) 한국환경농학회 2020 한국환경농학회지 Vol.39 No.4
BACKGROUND: Irrigation water is known to be one of the major sources of bacterial contamination in agricultural products. In addition, anti-microbial resistance (AMR) bacteria in food products possess serious threat to humans. This study was aimed at investigating the prevalence of foodborne bacteria in irrigation water and evaluating their anti-microbial susceptibility. METHODS AND RESULTS: Surface water (n = 66 sites) and groundwater (n = 40 sites) samples were collected from the Gyeongi and Gangwon provinces of South Korea during April, July, and October 2019. To evaluate the safety of water, fecal indicators (Escherichia coli) and foodborne pathogens (E. coli O157:H7, Salmonella spp., and Listeria monocytogenes) were examined. E. coli isolates from water were further tested for antimicrobial susceptibility using VITEK2 system. Overall, detection rate of foodborne pathogens in July was highest among three months. The prevalence of pathogenic E. coli (24%), Salmonella (3%), and L. monocytogenes (3%) was higher in surface water, while only one ground water site was contained with pathogenic E. coli (2.5%). Of the 343 E. coli isolates, 22.7% isolates were resistant to one or more antimicrobials (ampicillin (18.7%), trimethoprim-sulfamethoxazole (7.0%), and ciprofloxacin (6.7%)). CONCLUSION: To enhance the safety of agricultural products, it is necessary to frequently monitor the microbial quality of water.