흉고직경별 약제 수간주입구멍의 크기에 따른 주입구멍의 치유도 - 포스팜 수간주입구멍에 대한 반응 -

차병진(Byeong Jin Cha),윤정구(Jeong Koo Yun) 한국산림과학회 1995 한국산림과학회지 Vol.84 No.1

Responses of Pospam-injected chestnut and pine trees to drill wound were examined. Pospam was injected into the stem by just filling up the drill wound in September 10, 1993. The diameter of the injection wound were 0.5 and 1㎝. DBH of chestnut trees and pine trees injected were 10, 15, 20 and 10, 20, 30㎝, respectively. The results of tree response were examined in June, 1994. None of them showed any symptom of decay by the time. However, sapwood under the bark was remarkably discolored. The discoloration was more severe in 1㎝-injection wound than in 0.5㎝ one. The severity of discoloration was not differed between species and among DBH applied. The sapwood split was longer in 1㎝-injection wounded trees than in 0.5㎝ trees and longer in chestnut trees than in pine trees. From the split, callus grew out and almost closed the splits. In the trunk injection tested, the damage was mare severe in 1㎝ injection wound of chestnut trees than in any other combination.

Characteristics of Cucumber mosaic virus isolated from Zea mays in Korea

김미경,Byeong-Jin Cha,Hae-Ryun Kwak,Su Heon Lee,김정수,김국형,차병진 한국식물병리학회 2011 Plant Pathology Journal Vol.27 No.4

A virus causing mottle and stunt symptom on Zea mays was observed around Ulleng-do, Korea and identified as Cucumber mosaic virus (CMV-ZM) based upon biological,serological, and molecular characteristics. In host range studies, the CMV-ZM isolate produced local lesions on Datura stramonium, Vigna unguiculata,Cucurbita moschata, Chenopodium amaranticolor, Ch. quinoa, whereas this isolate produced systemic mosaic on Nicotiana tabacum cv. ‘Xanthi-nc’, Capsicum annuum,Solanum lycopersicum, Solanum melongena, Cucurbita pepo, and Z. mays. In addition, chlorotic local rings on inoculated leaves along with severe mosaic, malformation,and fern leaf symptoms on upper systemic leaves were shown in N. glutinosa plants. Complete nucleotide sequences of each genomic RNA segment was determined and compared to those of the other CMV strains. Comparison of the nucleotide sequence of 1a open reading frame (ORF) revealed approximately 89.2–92.4% sequence identity with each CMV subgroup IA and IB strain, while showing only 78% sequence identity with CMV subgroup II. Nucleotide sequence analysis of RNA2 ORFs revealed 85.3–97.6% sequence identity with subgroup I. In ORFs of RNA3, levels of nucleotide sequence identities were higher than 92–99.2% with CMV subgroup I and lower than 82% with CMV isolates of subgroup II. These results suggest that CMV-ZM isolate is more closely related to subgroup I than subgroup II and therefore, CMV-ZM isolate might be classified into as CMV subgroup I based on biological and molecular analysis.

Occurrence of Papaya ringspot virus Infecting Cucurbit Crops in Korea

Tae-Seong Jin(진태성),Sang-Mok Kim(김상목),Sug-Ju Ko(고석주),Su-Heon Lee(이수헌),Hong-Soo Choi(최홍수),Jin-Woo Park(박진우),Byeong-Jin Cha(차병진) 한국농약과학회 2009 농약과학회지 Vol.13 No.4

단문 : Bacillus subtilis에서 분비되는 Colletotrichum gloeosporioides 생장 저해물질 생산에 미치는 배양조건의 영향

조수진 ( Soo Jin Cho ),차병진 ( Byeong Jin Cha ),신광수 ( Kwang Soo Shin ) 한국균학회 2004 韓國菌學會誌 Vol.32 No.2

Antifungal Activity of Streptomyces pad anus isolate TH04 against Monilinia fructicola, Brown rot Fungus on Stone-fruits

임태헌,최용화,이동운,한상섭,차병진,Lim, Tae-Heon,Choi, Yong-Hwa,Lee, Dong-Woon,Han, Sang-Sub,Cha, Byeong-Jin The Korean Society of Pesticide Science 2008 농약과학회지 Vol.12 No.3

복숭아 미이라 과일로부터 복숭아 잿빛무늬병 Monilinia fructicola에 대하여 강한 항균활성을 보이는 방선균 Streptomyces padanus TH04를 분리하였다. TH04 균주의 배양 추출물 1%를 함유한 배지에서의 균사생육 및 포자발아는 시험한 M. fructicola의 strain에 따라 각각 $79.8{\sim}81%$와 $73.9{\sim}75.8%$ 억제되었다. 병원균과 TH04 균주 초기접종 밀도를 0.01%, 0.1% 및 1%로 달리하여 동시배양 한 결과, 항균활성은 선발 방선균의 밀도에 따라 $7.5%{\sim}94%$로 나타났다. 사과(품종; 후지)를 이용한 조추출물의 항균활성은 0.1% 처리구 85.9%, 1%처리구 100%로 나타났다. 항균활성 물질 생산, 안정성 및 제형화에 관한 연구가 이루어질 경우 선발한 Streptomyces padanus TH04는 생물학적 방제제로의 개발 가능성 있을 것으로 생각된다. The Streptomyces padanus isolate TH04, isolated from mummified peaches, showed strong antifungal activity to Monilinia fructicola. The inhibition activity of the isolate TH04 to mycelial growth and spore germination at 1% concentration of sub-antifungal powder made from culture suspension (CS) was ranged from 79.8% to 81.0% and from 73.9% to 75.8% to M. fructicola four strains, respectively. In the test of antifungal activity in mixed culture of the isolate and M. fructicola, inhibition rate was 7.5%, 86.8% and 94.0% in 0.01, 0.1, and 1% concentration of CS containing bacterial cell of the isolate, respectively. On apples (cultivar; Fuji), the control values of the isolate TH04 crude filtrates (0.1 and 1%) were 85.9% and 100%, respectively. The results suggest that the isolate TH04 indicate development possibility as biocontrol agent of brown rot caused by M. fructicola with the study on delivery method and fermentation condition to produce an antifungal compound.

PCR-SSCP 분석법에 의한 뽕나무 오갈병 파이토플라스마의 유전변이 검출기법

한상섭 ( Sang Sub Han ),차병진 ( Byeong Jin Cha ),성규병 ( Gyoo Byung Sung ) 한국산림과학회 2006 한국산림과학회지 Vol.95 No.6

Nested-PCR법과 Competitive PCR법을 이용한 뽕나무 오갈병(MD) Phytoplasma의 검출과 밀도변화

채승민 ( Seung Min Chae ),이솔 ( Sol Lee ),차병진 ( Byeong Jin Cha ),이혁인 ( Hyok In Lee ),한상섭 ( Sang Sub Han ) 한국산림과학회 2011 한국산림과학회지 Vol.100 No.4

The detectable levels and population fluctuations of phytoplasmas infecting dwarf mulberry trees were investigated using nested-PCR and competitive-PCR methods. Samples of five different types were studied : A. petiole of a leaf that displays dwarf symptoms, B. petiole from apparently healthy leaf residing on a branch also supports a leaf with dwarf symptoms, C. the branch portion that supports a leaf with dwarf symptoms, D. the leaf petiole from healthy appearing leaves on branch with no dwarf symptoms, and branch portion of branch with no dwarf symptoms, E. the rootlets of trees with dwarf symptoms. These 5-parts were collected from each tree during June - April, once in every two months. The phytoplasma was detected from all parts of collected mulberry samples during all seasons using nested-PCR with AS-1/AS-2 primer pairs. The phytoplasma was detected until 104 dilution using direct-PCR method, but it was detected until 1013 dilution by the nested-PCR method. The density of pytoplasma was found to be 7.94×1018-1012copies/μL in mulberry trees. The density of phytoplasma was observed throughout the year in all samples of mulberry trees. The highest rates of phytoplasma was found in the samples B and C during the early growing season followed by the sample A and D during the dormant season. Samples C and E displayed the highest phytoplasma density followed sample D. The density of phytoplasma appeared stable during all the seasons for samples C and A. The result of the present study demonstrates the utility of nested-PCR and competitive-PCR for detection and determination of population fluctuations of phytoplasmas in plant tissues.

고추의 발아 및 역병 발생에 미치는 인공산성비의 영향

차병진 한국환경농학회 1996 한국환경농학회지 Vol.15 No.2

인공산성비의 pH가 낮아질수록 고추씨의 발아에 걸리는 시간은 단축되었으며 발아율은 향상되었으나 어린 싹에게는 매우 치명적인 영향을 미쳤다. 어린 고추모는 이미 자란 식물체에 비하여 산성비에 훨씬 더 민감하게 작용하였다. 산성비의 전형적인 가시피해증상은 잎에 나타나는 흰색 반점이었으며, 빗물의 pH가 낮을수록 더 빨리 더 심하게 나타났다. 고추의 역병은 일반적으로 인공산성비 처리구에서 대조구보다 빨리 발병하였으나, 인공산성비의 pH가 아주 낮을 때는 다른 처리구에 비하여 오히려 병의 발생이 줄어들었다. 인공산성비 처리중에 역병을 접종하였을 때는 처리전에 접종하였을 때보다 병이 더 빨리, 더 심하게 나타났다. 역병의 발생이 가장 심했던 것은 역병의 접종시기와는 관계없이 pH 4.0 또는 4.5인 빗물을 처리하였을 때였다. Simulated acid rain(SAR) treatment caused a lethal effect on the newly germinated seedlings of Capsicum annum cv. Komyung even though the germination rate of the seeds was stimulated by the treatment of SAR. Young germinated seedlings were much more sensitive to SAR than the already-grown seedlings. The typical symptom caused by SAR was white spot or lesion on the leaf and appeared readily and severely at the low pH of SAR. Generally, Phytophthora rot was severer in SAR treatments than in control. However, the disease was less severe in lower pH of the SAR treatment than in higher pH. Pathogen inoculation following the SAR treatment increased the disease. The severest Phytophthora rot was observed in the SAR treatment of pH 4.0 or 4.5 regardless of the inoculation time.

여러수준의 산성비가 고추씨의 발아율 및 모 생육에 미치는 영향

차병진,이재춘 충북대학교 농업과학기술연구소 1994 農業科學硏究 Vol.11 No.2

In the experiment to determine the effect of simulated acid rain(SAR) of various pH on the germination rate and the growth of Capsicum annum cv. Komyung(red pepper), the low pH of SAR accerlated the grrmination of the seeds even though the effect of pH was not statistically significant. After 10 days of SAR treatment, soil pH dropped to a great extent. There was, however, little change in tap water treatment(control) . In SAR of low pH(2.5, 3.0), soil pH was lower than that of control after 10 days of treatment, but, it became lower after 20 days of treatment in high pH(3.5, 4.0, 4.5) SAR. Visible symptoms caused by SAR were white spot and marginal necrosis of the leaves. The lower the pH of SAR, the more the number of spots per leaf and the number of spotted leaves. Decrease in pH of SAR increased the severity of damage and reduced the period that the first visible symptom appeared. Effect of SAR on the DW/FW ratio of red pepper seedlings was not significant. Either in dry weight or fresh weight, root/shoot ratio of the seedlings, however, varied significantly by the pH of SAR. That is to say, root growth of SAR-treated seedlings was inhibited compared to that of tap water-treated seedlings.

인공산성비가 고추 생육 및 탄저병 발생에 미치는 영향

박세희,차병진 충북대학교 농업과학기술연구소 2001 農業科學硏究 Vol.18 No.-

Simulated acid rain(SAR) of various pH was made with the mixture of sulfuric acid and nitric acid (2:1, v/v) and treated to pepper (variety: Nokkwang) once a day for 10 successive days to investigate the effect of SAR on the occurrence of pepper anthracnose caused by Colletotrichum gloeosporioides. After finishing treatment of SAR, contacting angle of fruit surface for water drop increased as the pH of SAR increase. It means that the decrease of pH of SAR caused more serious erosion of cuticle wax on the plant surface. Actually, SAR of pH 4.0 caused a visible symptom of white small spots on the leaf and this symptom appeared more seriously and frequently when the pH of SAR was lower than 4.0. SAR also affect the yield of pepper plant and the number of pepper fruits per plant decreased by the decrease of the pH of SAR. However, the growth of the anthracnose fungus Colletotrichum gloeosporioides was not much varied from pH 3.0 to pH 6.0, and it seemed that SAR seldom affect the growth of C. gloeosporioides. The occurrence of anthracnose reached to its lowest at SAR of pH 3.5 and highest at SAR of ph 6.0. The occurrence rate of anthracnose decreased gently by the decrease of the pH of SAR and showed similar pattern with the contacting angle. Therefore, it seemed that there must be a certain relationship between the occurrence rate of anthracnose and the thickness of wax layer of leaf surface.