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      • KCI등재

        Efficacy of Different Nematicidal Compounds on Hatching and Mortality of Heterodera schachtii Infective Juveniles

        Kim, Jeongeun,Mwamula, Abraham Okki,Kabir, Faisal,Shin, Jin Hee,Choi, Young Hwa,Lee, Jae-Kook,Lee, DongWoon The Korean Society of Pesticide Science 2016 농약과학회지 Vol.20 No.4

        Effect of nematicidal compounds on hatchability of sugar beet cyst nematode, Heterodera schachtii and its infective juveniles was investigated. The sugar beet cyst nematode was isolated from Chinese cabbage field in Samcheok in Korea. Acute toxicity of nematicidal compounds against infective juveniles was also tested to find the $LC_{50}$ by exposing juveniles to given dilutions of each compound. Hatchability and mortality of infective juveniles of H. schachtii were influenced by nematicidal compounds (Fluopyram 40% SC, imicyafos 30% SC, fosthiazate 30% SC, abamectine 1.68% SC, terthiophene, and Eclipta prostrata extract). Fluopyram and imicyafos yielded the lowest rates of hatching. Total hatched infective juveniles were significantly different among nematicidal compounds. Positive correlation in percentage reduction of hatching was observed in fluopyram. Furthermore, the highest mortality was also observed in the treatments of fluopyram and imicyafos ($LC_{50}$ of 0.0543 and 0.0178 ppm respectively). The study, therefore, demonstrated available alternative nematicidal compounds which could be used in the control of H. schachtii.

      • KCI등재

        Fenton Oxidation of Bisphenol A using an Fe<sub>3</sub>O<sub>4</sub>-coated Carbon Nanotube: Understanding of Oxidation Products, Toxicity and Estrogenic Activity

        Cho, Il Kyu,Park, Byung-Jun,Chung, Kyu Hyuck,Li, Qing X.,Kan, Eunsung The Korean Society of Pesticide Science 2017 농약과학회지 Vol.21 No.3

        This study focused on Fenton oxidation of bisphenol A using a $Fe_3O_4-coated$ multi-walled carbon nanotube ($Fe_3O_4$/MWCNT), from which oxidation products, acute toxicity and estrogenic activity were assessed. The final effluents showed no biological toxicity to 24 h born Daphnia magna and negligible in-vivo estrogenic activity. The major oxidation productions generated from heterogeneous Fenton oxidation of bisphenol A included 4-hydroxyacetophenone, 4-hydroxybenzoic acid, catechol and hydroquinone. Therefore, Fenton oxidation of bisphenol A using $Fe_3O_4$/MWCNT with a low dose of $H_2O_2$ led to high removal of bisphenol A with negligible aquatic toxicity and estrogenic activity. It would be a cost-effective solution for treatment and reuse of emerging contaminant-containing wastewater and water.

      • KCI등재

        Exported Strawberry Gray Mold Decay Related Spore Density and Disease Incidence in Cultivation Field

        Jeon, Chang-Wook,Kim, Da-ran,Park, Jung Jun,Kang, Nam Jun,Kwak, Youn-Sig The Korean Society of Pesticide Science 2017 농약과학회지 Vol.21 No.2

        Exports of strawberries are currently expanding and have risen by 65% in the last few years. However, the occurrence of gray mold disease in packed strawberries has emerged. Gray mold disease, caused by Botrytis cinerea, leads to the decomposition of strawberries, reducing the total harvesting of the fruit by up to 50%. In this study, to determine the correlation between cultivation fields and a packing center of disease incidence and spore density, investigation were conducted for two consecutive years. The strawberry cultivation fields showed the highest disease incidence from March to May in both years. However, in both fields the pathogen spores in the air showed similar density during cultivation periods as $10^5cfu/L$ of air in the first year and $10^4cfu/L$ of air for the second year. In the packing center, the spore density patterns were similar to those in the fields. Temperature and humidity emerged as having the most correlation with incidence of the disease in the fields. The major finding in this study was that the source inocula of packed strawberry were derived from the cultivation fields. The findings can be utilized to effectively control gray mold decay in export strawberry production.

      • KCI등재

        Determination of Cyhalofop-butyl and its Metabolite in Water and Soil by Liquid Chromatography

        헴리나,최정희,유학,카이사티아,심재한,Hem, Lina,Choi, Jeong-Heui,Liu, Xue,Khay, Sathya,Shim, Jae-Han The Korean Society of Pesticide Science 2008 농약과학회지 Vol.12 No.4

        실험실 내 조건에서, 물과 토양 중 cyhalofop-butyl과 그 대사물질인 cyhalofop acid를 위한 잔류분석법이 고감도에서도 간단하고 매우 효과적으로 개발되었다. 물과 토양 중 cyhalofop-butyl과 cyhalofop acid를 분석하기 위하여 액액분별추출과 silica gel chromatographic 정제를 수행하였으며 HPLC-UV를 이용하여 정성/정량하였다. cyhalofop-butyl의 회수율은 2 가지 농도에서 3 반복 수행하여 각각 82.5-100.0%와 66.7-97.9%이었고, 검출한계와 최소검출량은 두 시료에서 모두 0.02 ppm과 10 ng이었다. Cyhalofop acid의 회수율은 물과 토양에서 각각 80.7-104.8%와 76.9-98.1%이 었으며, 검출한계는 각각 0.005 ppm과 0.01 ppm이었고 최소검출량은 두 시료에서 모두 2 ng이었다. Cyhalofop-butyl의 반감기는 물과 토양에서 각각 4.14와 6.6 day였다. 개발되어진 본 시험법은 cyhalofop-butyl의 30% 유탁제를 처리한 물과 토양에서 그 잔류량을 분석하기 위하여 성공적으로 적용되었다. In this study, a simple, effective, and sensitive method has been developed for the quantitative residue analysis of cyhalofop-butyl and its metabolite cyhalofop acid in water and soil when kept under laboratory conditions. The content of cyholofop-butyl and cyhalofop acid in water and soil was analyzed by first purifying the compounds through liquid-liquid extraction and partitioning followed by Silica gel (adsorption) chromatography. Upon the completion of the purification step the residual levels were monitored through high-performance liquid chromatography (HPLC) using a UV absorbance detector. The recoveries of cyhalofop-butyl from three replicates spiked at two different concentrations ranged from 82.5 to 100.0% and from 66.7 to 97.9% in water and soil, respectively. The limit of detection and minimum detection level of cyhalofop-butyl in water and soil was 0.02 ppm and 10 ng, respectively. The recoveries of cyhalofop acid ranged from 80.7 to 104.8% in water and from 76.9 to 98.1 % in soil. The limit of detection of cyhalofop acid was 0.005 ppm in water and 0.01 ppm in soil, while the minimum detection level was 2 ng both in water and soil. The half-live of cyhalofop-butyl was 4.14 and 6.6 days in water and soil, respectively. The method was successfully applied to evaluate cyhalofop-butyl residues in water and soil applied aj. 30% emulsion, oil in water (EW) product.

      • KCI등재

        Antifungal Activity of Streptomyces pad anus isolate TH04 against Monilinia fructicola, Brown rot Fungus on Stone-fruits

        임태헌,최용화,이동운,한상섭,차병진,Lim, Tae-Heon,Choi, Yong-Hwa,Lee, Dong-Woon,Han, Sang-Sub,Cha, Byeong-Jin The Korean Society of Pesticide Science 2008 농약과학회지 Vol.12 No.3

        복숭아 미이라 과일로부터 복숭아 잿빛무늬병 Monilinia fructicola에 대하여 강한 항균활성을 보이는 방선균 Streptomyces padanus TH04를 분리하였다. TH04 균주의 배양 추출물 1%를 함유한 배지에서의 균사생육 및 포자발아는 시험한 M. fructicola의 strain에 따라 각각 $79.8{\sim}81%$와 $73.9{\sim}75.8%$ 억제되었다. 병원균과 TH04 균주 초기접종 밀도를 0.01%, 0.1% 및 1%로 달리하여 동시배양 한 결과, 항균활성은 선발 방선균의 밀도에 따라 $7.5%{\sim}94%$로 나타났다. 사과(품종; 후지)를 이용한 조추출물의 항균활성은 0.1% 처리구 85.9%, 1%처리구 100%로 나타났다. 항균활성 물질 생산, 안정성 및 제형화에 관한 연구가 이루어질 경우 선발한 Streptomyces padanus TH04는 생물학적 방제제로의 개발 가능성 있을 것으로 생각된다. The Streptomyces padanus isolate TH04, isolated from mummified peaches, showed strong antifungal activity to Monilinia fructicola. The inhibition activity of the isolate TH04 to mycelial growth and spore germination at 1% concentration of sub-antifungal powder made from culture suspension (CS) was ranged from 79.8% to 81.0% and from 73.9% to 75.8% to M. fructicola four strains, respectively. In the test of antifungal activity in mixed culture of the isolate and M. fructicola, inhibition rate was 7.5%, 86.8% and 94.0% in 0.01, 0.1, and 1% concentration of CS containing bacterial cell of the isolate, respectively. On apples (cultivar; Fuji), the control values of the isolate TH04 crude filtrates (0.1 and 1%) were 85.9% and 100%, respectively. The results suggest that the isolate TH04 indicate development possibility as biocontrol agent of brown rot caused by M. fructicola with the study on delivery method and fermentation condition to produce an antifungal compound.

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