Dietary composition of two coexisting bat species, Myotis ikonnikovi and Plecotus ognevi, in the Mt. Jumbong forests, South Korea

주성배,안인정,김선숙 한국생태학회 2023 Journal of Ecology and Environment Vol.47 No.4

Background: Many insectivorous bats have flexible diets, and the difference in prey item consumption among species is one of the key mechanisms that allows for the avoidance of interspecies competition and promotes coexistence within a microhabitat. In Korea, of the 24 bat species that are known to be distributed, eight insectivorous bats use forest areas as both roosting and foraging sites. Here, we aimed to understand the resource partitioning and coexistence strategies between two bat species, Myotis ikonnikovi and Plecotus ognevi, cohabiting the Mt. Jumbong forests, by comparing the differences in dietary consumption based on habitat utilization. Results: Upon examining their dietary composition using the DNA meta-barcoding approach, we identified 403 prey items (amplicon sequence variants). A greater prey diversity including Lepidoptera, Diptera, Coleoptera, and Ephemeroptera, was detected from M. ikonnikovi, whereas most prey items identified from P. ognevi belonged to Lepidoptera. The diversity index of prey items was higher for M. ikonnikovi (H’: 5.67, D: 0.995) than that for P. ognevi (H’: 4.31, D: 0.985). Pianka’s index value was 0.207, indicating little overlap in the dietary composition of these bat species. Our results suggest that M. ikonnikovi has a wider diet composition than P. ognevi. Conclusions: Based on the dietary analysis results, our results suggests the possibility of differences in foraging site preferences or microhabitat utilization between two bat species cohabiting the Mt. Jumbong. In addition, these differences may represent one of the important mechanism in reducing interspecific competition and enabling coexistence between the two bat species. We expected that our results will be valuable for understanding resource partitioning and the coexistence of bats inhabiting the Korean forests.

Identification of bird species and their prey using DNA barcode on feces from Korean traditional village groves and forests (maeulsoop)

주성배,박상규 한국통합생물학회 2012 Animal cells and systems Vol.16 No.6

A DNA barcode based on 648 bp of cytochrome c oxidase I (COI) gene aims to build species-specific libraries for animal groups. However, it is hard to recover full-length (648 bp) barcode gene from environmental fecal samples due to DNA degradation. In this study, we designed a new primer set (K_Bird), which amplifies a 226 bp fragment targeted an inner position of full-length COI barcode based on 102 species of Korean birds to improve amplification success, and we attempted to identify bird species from 39 avian fecal samples collected during 4 months from Jinan,South Korea. Simultaneously, we conducted a dietary analysis using a universal DNA mini-barcode (Uni_Minibar)from same fecal samples. In silico analysis on newly designed mini-barcode represented that genetic distances were 0.5% in species and 9.1% in genera. Intraspecific variations of 149 species out of 174 species (86%) between Korea and North America were within the threshold (5.3% threshold in this study). From environmental fecal samples collected in Jinan, we identified seven avian species, which have high similarity (99100%) with registered COI sequences in GenBank. Eight kinds of prey species, such as moth, spider, fly, and dragonfly, were identified in dietary analysis. We suppose that our strategy applying mini-barcode for environmental fecal samples, might be a useful and convenient tool for species identification and dietary analysis for birds.

고압분사주입공법(J.S.P.)의 소개

주성배 한국어촌어항협회 1990 어항어장 Vol.11 No.-

후리젯 공법-자유단면형 다중관 고속분사 교반공법-

심재구,주성배 한국지반공학회 1998 지반 : 한국지반공학회지 Vol.14 No.4

멸종위기 개리 (Anser cygnoides)의 월동기 서식지 이용과 먹이원

최유성,주성배,김면식,한동욱,정길상 한국수생태학회(구 한국하천호수학회) 2017 생태와 환경 Vol.50 No.2

We surveyed the habitat use and food materials of the endangered Swan Goose (Anser cygnoides) during the wintering period at the Seocheon tidal flat. The bird wintered about six months from October 2014 to early April 2015 at the flat. They showed a remarkable change in choosing their feeding habitat and prey. They stayed entirely at sedge grass patches on Songrim tidal flat during the early wintering period (October), while after November they gradually moved to Janggu bay and used rice paddy fields as well as sedge patches on the tidal flats as their feeding site. The dietary analysis showed the sedge grass (Bolboschoenus sp., Cyperaceae) was the main dietary source during the entire wintering period. Interestingly, the proportion of Cyperaceae on feces decreased slightly over wintering time, while the proportion of Fabaceae increased relatively since November. These results suggest that the Swan Geese switched their habitat and food sources in response to the change of food availability. The Seocheon tidal flat area is the important wintering site for Swan Geese and sedge grass patches in the area need to be managed as the main feeding area for the wintering Swan Geese.

철원 민간인통제지역 해제 이후 인공시설물 증가에 따른 두루미류 분포변화

유승화,주성배,이기섭,김수호,김동원,김화정,김진한 한국환경영향평가학회 2020 환경영향평가 Vol.29 No.1

This study aims to evaluate the effect of artificial facilities constructed after the release of the civilian controlled zone (CCZ) in Cheorwon on the inhabitation of cranes. The study site was released from the CCZ in 2012, and several barns have been built since 2017. The average number of Red-crowned Cranes was 9.3±4.3 individuals (±Standard Deviation) in the period before the release of the CCZ from 2009 to 2012, and it decreased by ca. one third of Red-crowned Crane’s average individuals were showing 3.5±0.5 individuals after release. The average number of White-naped Cranes also appeared to be decreased by ca. 90% from 63.3±24.6 to 6.0±6.0 individuals. This results suggest that the construction of greenhouses and barns after the releasing of the CCZ affected the decrease of the population of these two crane species. In the case of Red-crowned Crane, the core area tended to move away from the area where barns were built. However, the distribution range of Red-crowned Cranes did not change significantly we expected because the study area was not their favorite place since 2012 when the area was released from the CCZ. The density of the White-naped Crane was relatively high even in the area where greenhouses were built, but after 2017 when the barn was built intensively, core habitat of crane became shrink and fragmented. These results suggest that both Red-crowned Cranes and White-naped Cranes response sensitively against habitat change due to the construction of barns. Since the release of the CCZ, the construction of the greenhouse has been influenced on the distribution of cranes, but subsequently, the construction of the barn seems to have caused a bigger change in their core habitat because the passage of people to barns is more frequent than the greenhouses. 본 연구는 철원에서 민간인통제지역 해제 이후 시공된 인공시설물이 두루미류의 서식에 미치는 영 향을 평가하였다. 연구 장소는 2012년에 민간인통제지역에서 해제되었고 2017년부터는 축사가 만들어진 장소를 대상으로 하였다. 두루미의 평균 서식 개체수는 민간인통제지역 해제 전(2009년-2012년)에는 9.3±4.3 개체 이었으며, 해제 이후 3.5±0.5 개체가 되어 1/3 수준으로 감소하였다. 재두루미는 민간인통 제지역 해제 전과 후에 각각 63.3±24.6 개체, 6.0±6.0 개체로 조사되어 90% 가량이 감소하였다. 이 결과 는 민간인통제지역의 해제 이후 비닐하우스, 축사의 건립이 전반적으로 두루미의 서식개체수 감소에 영향 을 준 것으로 판단된다. 두루미의 경우 축사가 건립된 지역으로부터 핵심지역이 멀어지는 경향이 나타났다. 하지만 연구지역은 2012년 민간인통제지역이 개방된 시기부터 선호되는 지역이 아니었기 때문에 두루미 의 분포범위 변화는 예상보다 크지 않았다. 재두루미의 경우 비닐하우스가 건립된 지역에서도 상대적으로 높은 밀도로 분포하였지만, 축사가 집중적으로 건설된 2017년 이후에는 핵심서식지가 위축되고 파편화되 는 변화가 나타났다. 이러한 결과는 두루미와 재두루미 모두 축사의 신축으로 인한 서식환경 변화에 대해 민감하게 반응한 것으로 보인다. 반면 민간인통제지역 개방 이후 비닐하우스가 건립되어 영향을 주었지만 축사의 건립은 핵심서식지에 대해 더 큰 변화를 유발한 것으로 나타났으며 이는 사람의 통행 등이 비닐하 우스에 비해 축사가 더 빈번하기 때문으로 사료된다.

중국 내몽골 훼손된 건조지 초원의 인공식재에 의한 복원된 식물군락의 천이 및 토양 특성의 변화

이승혁,유영한,주성배,이은주 한국환경생태학회 2018 한국환경생태학회 학술대회지 Vol.2018 No.2

반초원의 식생이 훼손된 중국 내몽골의 간주얼 지역에서 인위적인 식물의 도입에 의하여 서로 다른 연도에 복원된 식물군락의 천이와 토양의 특성 변화를 조사하였다. 간주얼 지역의 식물군집(식피율, 식물 수고와 식물 종다양성(종수)등)의 결과들은 복원지소들이 비복원지보다 높게 나타났지만, 주변의 정상적인 대조지소들보다는 낮은 값을 보였다. 이러한 결과는 이 지역에서 생태복원으로 인하여 생태계의 구성요소가 꾸준하게 발전하고 있음을 뜻한다. 복원이 가장 오래된 2005년 지소의 경우, 식물종구성이 유사한 대조지 사이트인 초지지역의 것들과 식피율이나 식생고가 유사한 결과들을 보여 복원의 효과를 긍정적 볼 수 있으나, 식물다 양성 측면에서는 아직 대조지 초지의 것보다 높지 않았다. 2017년의 경우, 2014년 조사 시작 이래 가장 많은 종들이 고사하여 주요 우점종들의 생육이 불안정한 상태를 가시적으로도 확인할 수 있는 정도로 현지조사의 결과들이 이를 반영하고 있다. 2008년 복원지 장자송의 소지생존 성장률은 47%로 작년(100%)보다 낮아졌으며, 조사대상인 20개체 중 5개체가 고사하였다. 군락 내 식생고(식물체높이)의 결과에서는 복원지들이 모두 낮아진 결과를 보였는데, 이는 장자송, 캐나다포플러 등 주요 우점종들이 고사하여 나타난 결과이다. 이렇게 많은 식물이 고사함에도 불구하고 하층에 살아 남아 생존하는 동일수종 및 다른 식물종들은 경쟁상대가 줄어들면서 오히려 더 활력이 좋아지는 결과를 나타내기도 하였다. 이러한 결과는 복원지에서 정착된 식생의 발달은 진행 중이나 그 항목에 따라서 외부의 물리적인 환경(가뭄 등)과 같은 요인에 의하여 영향을 크게 받고 있음을 의미 한다. 식생군락과 환경요인과의 분석(CCA)결과는 비생육기 온도(11월~2월), 생육기온도(6월~8월), 토양유기물, TN, 유효인산 그리고 산화칼륨이 영향을 주는 외부의 주요 비생물학적 환경인자로 나타났다.

Allelopathic Inhibition Effects of Myriophyllum spicatum on Growths of Bloom-Forming Cyanobacteria and Other Phytoplankton Species in Coexistence Experiments

정선아,양동우,주성배,박상규 한국식물학회 2021 Journal of Plant Biology Vol.64 No.6

Many studies have attempted to find measures for control of cyanobacterial harmful algal blooms (cyanoHABs) caused by Microcystis, Anabaena, and other bloom-forming plankton species. We have investigated allelopathic inhibition of the submerged macrophyte Myriophyllum spicatum on four phytoplankton species of two taxonomic groups: Chlorophyta Selenastrum capricornutum, Scenedesmus obliquus, and cyanobacteria Microcystis aeruginosa (different strains for toxic, non-toxic, the North Han River originated (NHR) and colonies) and Anabaena circinalis. Inhibitions of unicellular cyanobacteria M. aeruginosa were over 50% for three consecutive days from the 3rd to the 5th day of the coexistence. M. spicatum even inhibited M. aeruginosa at a high initial concentration (1.1 mg L−1 Chl-a). Moreover, M. aeruginosa in a mixture of four phytoplankton species (S. capricornutum, S. obliquus, M. aeruginosa and A. circinalis) was selectively inhibited by M. spicatum. The inhibition of toxic, non-toxic, and NHR of Microcystis by M. spicatum were not significantly different. Colonial cyanobacteria strains were mostly not inhibited by M. spicatum.

유부도 일대에 서식하는 검은머리물떼새 (Haematopus ostralegus osculans)의 번식기 섭식지 유형에 따른 섭식행동과 먹이원

이상연,최유성,주성배,정길상,유영한 한국수생태학회(구 한국하천호수학회) 2018 생태와 환경 Vol.51 No.2

번식기 유부도 일대에 서식하는 검은머리물떼새(Haematopus ostralegus osculans) 성체와 미성체의 섭식 능력 비교

이상연,유영한,정길상,최유성,주성배 한국환경생물학회 2019 환경생물 : 환경생물학회지 Vol.37 No.4

During the breeding season, both adults and immature Eurasian Oystercatchers (Haematopus ostralegus osculans) were observed to feed on prey, such as gastropods (Umbonium thomasi) and bivalves, at the tide water line around Yubu island in Korea. To determine differences in the foraging efficiency between the adult and immature birds, we recorded the feeding behavior using digiscoping with a telescope and a smartphone. We compared the pace, the prey search rate, the proportion of prey search techniques, the feeding success rate, the handling time, and the heads-up rate between the adult and immature birds. There were significant differences in the proportion of prey search techniques when gastropods were consumed and in the prey search rate and heads-up rate when bivalves were consumed but there were no significant differences in feeding success rates. Therefore, although the immature birds on Yubu Island were inferior to adults during the breeding season and they lacked the proficiency to locate prey, their overall foraging efficiency was similar to adults. This was considered to be due to the continuous eating of bivalves during the wintering season, which requires a high level of foraging efficiency. 번식기 유부도 일대의 조수 경계부에서는 검은머리물떼새(Haematopus ostralegus osculans) 성체와 미성체가 한데 모여 복족류(서해비단고둥, Umbonium thomasi)와 이매패류를 섭식하는 것이 관찰된다. 본 연구는 성체와 미성체 간의 섭식 능력에 차이가 있는지 알아보고자 해당 먹이원을 섭식하는 개체들의 행동을 영상으로 녹화한 후 성체와 미성체로 구분하여 단위 시간당 걸음수, 먹이원 탐색시도횟수 및 탐색방법 구성비율, 섭식성공횟수, 조작시간, 머리 들어 올리기횟수를 비교하였다. 서해비단고둥을 섭식할 때 먹이원 탐색방법의 구성 비율, 이매패류를 섭식할 때 먹이원 탐색시도횟수와 머리 들어올리기횟수에서 유의한 차이를 나타냈으나, 단위 시간당 걸음수, 탐색시도횟수, 섭식성공횟수는 차이가 없었다. 결과적으로 번식기 유부도 일대에 서식하는 검은머리물떼새 미성체가 성체에 비해 비록 열세이고, 먹이원의 위치를 파악하는 능력이 부족하지만, 전반적인 섭식 능력은 성체와 유사한 수준임을 의미한다. 월동기간 동안 고도의 섭식 능력을 갖추어야 섭식이 가능한 이매패류를 지속적으로 섭식한 것에 기인한 것으로 판단된다.