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손경선(Kyung Sun Son),조해정(Hai Jeong Cho),김애경(Ae Kyoung Kim),이종진(Jong Jin Lee),한표성(Pyo Seong Han),김주옥(Ju Ock Kim),김선영(Sun Young Kim) 대한내과학회 1995 대한내과학회지 Vol.48 No.4
N/A Objectives: EGFR is a transmembrane molecule and is characterized by tyrosine protein kinase activity and ligand-stimulated autophosphorylation. It was found in various cell types including fibroblast and keratinocytes. EGFR appears to have an important role in the development and proliferation of certain human malignancies, including those of neuroglia, bladder, and breast. The heterogeneity of EGFR expression is known to be related with proliferating fraction, histological grade, and susceptibility of anti- cancer drugs, etc. We investigated the biologic significance of the expression of EGFR in lung cancer tissues. Methods: The expression of EGFR was assessed immunohistochemically on paraffin imbedded lung cancer tissues including 28 primary sites with 16 lymph nodes using monoclonal antibody and indirect peroxidase technique. Results: 1) EGFR expression of primary tumor in squamous cell carcinoma was higher than in the others. 2) EGFR expression of primary tumor in stage III was higher than in stage I and stage II. 3) EGFR expression rates of primary and metastatic sites were 60.7% and 61.1%, respectively. No significant difference between primary site and metastatic one was noticed. 4) EGFR expression rates in primary tumor with metastasis was 64.7% and without metastasis was 54.5%. 5) EGFR expression rates of lung cancer by nodal stage were 50% in N1 and 60% in N2. But significant difference among nodal stages was not noticed. 6) EGFR expression rates of lymph nodes according to EGFR positivity of primary tumor were 50% in lymph nodes that have negative steins in lungs and 60% in lymph nodes that have positive stains in lungs. 7) No significant difference in 12-month survival between positive group and negative group was noticed. Conclusion: EGFR expression of routinely processed tissue is a simple technique for the assessment of cell growth and terminal differentiation in non small cell lung cancer. Whether EGFR expression has an independent prognostic value and deserves special attention in pathobiological evaluation in lung cancer remains to be investigated from large series with longer follow - up and to be correlated with multiple biological markers.
제한병기 소세포폐암에서 흉부 방사선 치료의 도입 시기에 따른 치료 효과의 비교 분석
박상기 ( Sang Ki Park ),김근화 ( Geun Hwa Kim ),정성수 ( Seong Su Jeong ),신경상 ( Kyoung Sang Shin ),김애경 ( Ae Kyoung Kim ),조해정 ( Hai Jeong Cho ),서지원 ( Jee Won Suhr ),김재성 ( Jae Sung Kim ),조문준 ( Moon June Cho ),김주 대한결핵 및 호흡기학회 1996 Tuberculosis and Respiratory Diseases Vol.43 No.6
중합효소연쇄반응을 이용한 도말양성 폐결핵 환자에서의 결핵균 DNA 검출방법에 관한 연구
이종진,김애경,조해정,한표성,홍석철,김주옥,김선영 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2
Polymerase chain reaction (PCR) is the most sensitive and rapid method for the diagnosis of M.tuberculosis infection. Many reports have shown different methods for mycobacterial DNA extraction, but revealed many differences in simplicity and time-consumption. We studied which method was the best among 7 different ones for DNA extraction from 32 smear-positive sputa, using PCR of targeting 123bp DNA fragment of IS6110. The methods were the following : SDS-Microwave oven method, NaOH Lysis method, Triton X-100, Proteinase K method, Lysis buffer method. SDS-Proteinase K method, Bead Beater method, and Bead Beater/CTAB method. The amplification products were identified by electrophoresis on ethidium bromide-stained agarose gels, and confirmed by restriction enzyme, Southern blot hybridization, and DNA sequencing. All 32 cases were positive (100%) by the 7 different methods. It took 1 hour or more to detect DNA in SDS-Microwave oven method, NaOH Lysis method, and Bead beater/CTAB method. It took 2 hours or less in the others. SDS-Proteinase K method and Bead Beater/CTAB method were simpler and more convinient than the others. These results suggest that SDS-Proteinase K method and Bead Beater/CTAB method might be better than the others for the extraction of mycobacterial DNA in terms of simplicity and time-consumption, although all the methods were sensitive.