http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
조기웅,방지헌,홍혜원,박승일,이윤호 한국미생물학회 2002 미생물학회지 Vol.38 No.4
저온에서 최적 생육을 보이는 저온성 균주를 남극해양에서 분리하여 생화학적 특성 및 165 rRNA 염기서열로부터 Shewanella sp.에 속하는 균주로 동정하고 Shewanella sp. L93으로명명하였다. 본 균주에서 생산되는 저온성 세포외 단백질 분해 효소(extracellular protease)를 ammonium sulfate precipitation, High-Q column chromatography, 일차 gel permeation chromatography, BioScale Q2 ion exchange chromatography 및 gel permeation chromatography를 통하여 purification fold 19.3, yield 0.7 %로 정제하였고 그 특성을 조사하였다. A psychrotrophic bacterium was isolated from Antarctic marine sediment and identified as Shewanella sp. species based on the biochemical properties and 16S rRNA sequence, and designated as Shewanella sp. L93. Extracellular protease produced by this strain was purified through ammonium sulfate precipitation, High-Q column chromatography, first gel permeation chromatography, BioScale Q2 ion exchange chromatography and second gel permeation chromatography, and basic properties of this enzyme were investigated.
조기웅,이현재,심상철,Cho, Ki-Woong,Lee, Hyun-Jae,Shim, Sang-Chul 생화학분자생물학회 1986 한국생화학회지 Vol.19 No.2
박테리아의 생체 발광반응에서 hololuciferase와 분자 산소간의 반응으로 형성되는 monooxygenating중간체 (complex-I)가 여러가지 luciferase : FMN 복합체로부터 외부에서 가해준 $H_2O_2$에 의해 인위적으로 형성됨을 확인하였다. $H_2O_2$에 의한 complex-I의 형성은 luciferase : FMN복합체가 생성된 방식에 따라 그 효율을 달리하고 있으며 발광반응을 거쳐 생성된 복합체에서 가장 높은 효율을 보였고 과량의 긴사슬 알데히드와 $H_2O_2$ 존재하에서 complex-I의 지속적인 재형성으로 turnover의 성격을 보이는 발광현상을 야기시킬 수 있었으며 원래의 발광반응과 동일한 발광 spectrum을 보였다. The monooxygenating intermediate formed from the reaction of hololuciferase and oxygen is regenerated from the luciferase:FMN complex directly by exogeneous $H_2O_2$. This regeneration is much more efficient when the luciferase:FMN complex is generated from the luminescent reaction than when generated from equilibrium binding. The bioluminescence spectra from this regenerated intermediate are well matched with emission spectra of the normal reaction.
해양방선균으로부터 Haloperoxidase의 검색과 특성
조기웅,Cho, Ki-Woong 한국미생물학회 2008 미생물학회지 Vol.44 No.2
Haloperoxidase를 생산하는 미생물을 분리하기 위하여 국내 연근해와 남북극 등의 해양시료에서 분리된 방선균 균주를 대상으로 탐색을 수행하여 남해 백도 해조류 추출물로부터 분리된 한 종류의 방선균(#1460)에서 높은 haloperoxidase 활성이 확인되었다. 본 균주의 생리.생화학적 특성은 Streptomyces 속과 유사하며 생산되는 haloperoxidase는 세포 조 추출물로부터 ammonium sulfate precipitation, High-Q column chromatography, gel permeation chromatography, Hydroxyapetite chromatography 그리고 hydrophobic interaction chromatography를 통하여 42%의 수율과 purification fold 70으로 정제하였다. 본 효소의 최적 반응 pH는 7이고 pH 8에서 더 높은 안정성을 보여 $60^{\circ}C$에서 1시간 반응에 효소활성의 50%가 생존한다. 또 cyanide와 azide 이온에 의해 강한 저해현상을 보인다. In my search of microbial source of novel enzymes, a marine actinomycetes, A1460, producing haloperoxidase was isolated from macroalgae from south sea, Korea and studied for physiological and biochemical properties. The haloperoxidation reaction was followed by the bromination of phenol red in the presence of hydrogen peroxide and potassium bromide. The haloperoxidase was partially purified from the cell extract with $35\sim75%$ ammonium sulfate precipitation, High-Q anion exchange chromatography, gel filtration chromatography, hydroxyapetite chromatography and hydrophobic interaction chromatography to a yield of 42% and purification fold of 70. This enzyme showed relatively high heat stability without losing 50% of activity after 1 hr incubation at $60^{\circ}C$. The highest activity was found at $45^{\circ}C$, and the optimal pH was about pH 7, but higher stability was observed at pH 8. Azide and cyanide ion showed strong inhibition at less than 1 $\mu M$ level suggesting that the enzyme was Fe ion dependent haloperoxidase.
Isocratic HPLC Pump를 이용한 Amino Acid의 분석
조기웅,신종헌,정경화 한국산업미생물학회 2000 한국미생물·생명공학회지 Vol.28 No.3
Instead of using binary HPLC pump which is usually used for the generation of solvent gradient for the analysis of amino acid, an isocratic HPLC pump and conventional gradient maker were demonstrated to perform such analysis with much lower expenses and similar effciency.
Photobacterium fischeri 루시퍼라아제에 의한 아세트알데히드의 생체 발광 반응과 긴 사슬 화합물에 의한 촉진 현상
조기웅,이현재,심상철 ( Ki Woong Cho,Hyun Jae Lee,Sang Chul Shim ) 생화학분자생물학회 1987 BMB Reports Vol.20 No.1
The bioluminescence of acetaldehyde catalyzed by bacterial luciferase is stimulated by long chain alkyl compounds. The degree of stimulation is dependent upon the chain length of alkyl group and the properties of the polar head group, showing maximum at nonyl group and bromide. In the presence of the long chain aliphatic aldehydes, a synergistically stimulated luminescence was resulted. The acetaldehyde bioluminescence stimulated by long chain aliphatic compounds is compared with that of corresponding long chain aldehydes.
How Do Different Types of Parental Involvement Affect Academic Achievement?
조기웅,최예나 동의대학교 지방자치연구소 2022 공공정책연구 Vol.39 No.3
The relationship between parental involvement and students’ academic achievement has long been an issue, but the results of research have been inconsistent. We reinvestigated parental involvement and school performance from the more comprehensive public administration perspective of citizen participation and organization performance. After we categorized parent involvement into school and home factors using the data from the Korea Educational Development Institute (KEDI), we conducted a panel analysis. We found that student academic achievement depends on the type of parental involvement, supporting the current competing point of view on citizen participation. Grade management, private tutoring time, and money spent for tutoring had a positive influence on students’ academic achievement. On the other hand, direct parental involvement and parents’ expectations for education had a negative influence on academic achievement. Whereas prior studies explored only one effect of citizen participation or parental involvement, our study showed conflicting perspectives on the positive and negative effects of citizen participation on performance using the research on parental involvement and academic performance in South Korea.
박테리아 Lucifease 에 의한 생체 발광 반응에서의 고에너지 중간체에 관한 연구
조기웅,이현재 ( Ki Woong Cho,Hyun Jae Lee ) 생화학분자생물학회 1984 BMB Reports Vol.17 No.1
Reaction mode of bioluminescence mediated by bacterial luciferase from P. fischeri was studied by stopped-flow apparatus measuring the light intensity, and from the results, it was suggested that the bacterial bioluminescence reaction occurs by at least two step mechanism, i.e., the first step is the activation of molecular oxygen to form a flavin-hydroperoxy anion or oxiene by the luciferase bound reduced flavin, and the second step is the monooxygenation of aldehyde by the activated oxygen to form a three membered ring structure of dioxirane as the high energy intermediate, which in turn, transfers its energy intermolecularly to the lumazine containing blue fluorescence protein in vivo resulting the elevation of the lumazine to its electronic excited state coupled with the conversion of dioxirane to the corresponding carboxylic acid.